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http://dx.doi.org/10.7314/APJCP.2013.14.2.951

Saffron Reduction of 7,12-Dimethylbenz[a]anthracene-induced Hamster Buccal Pouch Carcinogenesis  

Manoharan, Shanmugam (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Wani, Shamsul Afaq (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Vasudevan, Krishnamoorthy (Department of Zoology, Faculty of Science, Annamalai University)
Manimaran, Asokan (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Prabhakar, Murugaraj Manoj (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Karthikeyan, Sekar (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Rajasekaran, Duraisamy (Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.2, 2013 , pp. 951-957 More about this Journal
Abstract
Our aim was to investigate the chemopreventive potential of saffron in DMBA-induced hamster buccal pouch carcinogenesis. Assessment was by monitoring the percentage of tumor bearing hamsters, tumor size as well as the status of detoxification agents, lipid peroxidation and antioxidants. Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting them with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We observed 100% oral tumor formation with severe histopathological abnormalities in all the hamsters treated with DMBA alone, activities of phase I and phase II detoxification enzymes, lipid peroxidation and antioxidants being significantly altered. Though oral administration of saffron completely prevented the formation of tumors, we noticed severe hyperplasia and dysplasia in hamsters treated with DMBA, suggesting that tumors might eventually develop. Oral administration of saffron return detoxification enzymes, lipid peroxidation and antioxidants to normal ranges. The chemopreventive potential of saffron thus is likely due to antioxidant properties and modulating effects on detoxification in favour of the excretion of carcinogenic metabolites during DMBA-induced hamster buccal pouch carcinogenesis.
Keywords
Antioxidants; chemoprevention; detoxification enzymes; oral cancer; saffron;
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