• 제목/요약/키워드: halo test

검색결과 53건 처리시간 0.026초

금속-도재관용 Ni-Cr합금의 조성변화와 소성단계에 따른 전단결합강도 (Observation of Shear Bonding Strength by Compositional Change and Firing Steps of the Ni-Cr Alloy for Porcelain Fused Metal Crown)

  • 조용완;홍민호;김원영;최성민;정인성
    • 대한치과기공학회지
    • /
    • 제35권4호
    • /
    • pp.353-358
    • /
    • 2013
  • Purpose: This study was observation shear bonding strength by compositional change and firing step of a Ni-Cr alloy for porcelain fused metal crown. The aim of study was to suggest the material for firing step of Ni71-Cr14 alloy to development of alloy for porcelain fused to metal crown. Methods: The test was on the two kinds of Ni-Cr alloy specimens. The surfaces of two alloys were analyzed by EDX in order to observe oxide characteristic. And the shear test was performed by MTS. Results: The surface property and oxide characteristic analysis of oxide layer, weight percentage of Element O within $Ni_{71}Cr_{14}$ alloy measured 23.32wt%, and $Ni_{59}Cr_{24}$ alloy was measured 23.03wt%. And the maximum shear bonding strength was measured 58.02MPa between $Ni_{59}Cr_{24}$ alloy and vintage halo(H4 group). Conclusion: The surface property and oxide characteristic three kind of Ni-Cr alloy was similar. and shear bonding strength showed the highest bonding strength in H4 specimens.

Rare Mating에 의한 양초효모에서의 glucoamylase 발현 균주 HCS 선별 및 특성 (Characterization of Brewing Yeast Expressing Glucoamylase Selected by Rare Mating.)

  • 최병주;장금일;김광엽
    • 한국미생물·생명공학회지
    • /
    • 제29권4호
    • /
    • pp.212-220
    • /
    • 2001
  • S. cerevisiae HBC52와 S, diataticus K114 의 rare mating 에 의해 개발된 HCS 균주들은 크기가 약 $13\mu\textrm{m}$ karyotype 분석결과 K114 균주에만 있는 약 1150kb 분자량을 가지는 염색체 band를 유지하였으며 전분을 분해하여 halo 를 형성하였다. Glucoamylase 활성은 약 2.7~3.4 unti/ml 를 가진 균주임이 밝혀졌으며 당 발효실험과 응집성 실험을 수행한 결과 HBC52 균주와 유사한 당 발효특성을 보이고 응집성 특성도 약응집성의 floculation type으로 비슷하였다. 그리고 HCS 균주의 포자형성과 피막형성 유무 실험에서는 양조효모인 HBC52 균주와 같이 포자가 형성되지 않았으며, 피막도 형성되지않았다. 균주들의 최종당도 실험은 HBC52균주가 약 68%의 발효수준을 나타냈고, HCS 균주들은 이 보다 높은 76~78%의 수준을 보였따. 즉 HBC52 균주가 최종당도($ 2.00^{\circ}$P)를 보인 반면 HCS 균주들은 ($0.7~0.93^{\circ}$P)를 보이는 결괄르 나타내어 맥주양조에서 low carbohydrate beer를 생산할 수있음이 확인되었다.

  • PDF

Pseudomonas fluorescens BB2 균주가 생산하는 단백질성 항생물질에 의한 효모 Candida 생육 억제 (Inhibition of yeast Candida growth by protein antibiotic produced from Pseudomonas fluorescens BB2)

  • 안경준
    • 미생물학회지
    • /
    • 제51권4호
    • /
    • pp.448-452
    • /
    • 2015
  • 효모의 생육을 억제하는 세균을 배추의 근권 토양에서 분리하였다. API 20NE test와 16S rRNA 유전자 염기서열 분석 결과 Pseudomonas fluorescens BB2로 동정되었다. P. fluorescens BB2 균주는 3%의 glucose가 포함된 YM 배지에서 $20^{\circ}C$로 배양하였을 때 효모에 대한 항생물질을 2차 대사산물로서 효과적으로 생산하였다. BB2 균주의 단백질성 항생물질은 ammonium sulfate에 의한 침전과 N-butanol 추출에 의해 농축되었으며, 효모의 생육을 억제하는데 Candida albicans KCTC 7965에 대한 minimal inhibitory concentration은 $10{\mu}g/ml$이었고, $80{\mu}g/ml$ 농도에서는 완전히 억제하였다. N-butanol 추출에 의한 친수성 분획은 Bacillus cereus ATCC 21366의 생육을 억제하였으며, chrome azurol S 평판배지에서 주황색 halo를 생성하므로 철과 결합하는 siderophore를 포함한다. 세포막을 통한 crystal violet의 흡수를 조사한 결과 효모 C. albicans에 대한 소수성 항생물질 $60{\mu}g/ml$의 농도에서는 대조군에 비해 막 투과성이 약 9% 증가하였다. P. fluorescens BB2 균주가 생산하는 항생물질은 효모 Candida의 생육을 억제하는 antimicrobial peptide의 일종으로 보이며, 이는 Pseudomonas 속에서는 처음으로 보고되는 것이다.

나트륨-물 반응에 의한 5Cr-1Mo Steel 시편의 부식특성 (Corrosion Characteristics of a 5Cr-1Mo Steel Specimen by Sodium-Water Reaction)

  • 정경채;정지영;박진호;황성태;김의식
    • 공업화학
    • /
    • 제9권7호
    • /
    • pp.1023-1029
    • /
    • 1998
  • 5Cr-1Mo steel을 이용하여 나트륨 분위기에서 미량 물 누출 실험을 수행하였다. 시편에서 미량 물 누출로 인한 누출경로의 완전 re-open time은 129분으로 나타났고, 그 크기는 직경 2mm를 나타냈다. 누출경로는 re-open되기 전에 누출부위를 중심으로 halos현상을 형성하였으며, halos의 크기와 실제 re-open크기와는 다르게 나타났다. 나트륨-물 반응으로 인한 재질의 부식은 나트륨부위로부터 시작되었으며, steam 부위에서는 부식이 발생하지 않았다. 시편 누출부위를 AES로 분석한 결과 Cr의 segregation이 가장 많이 나타났으며, SEM과 EPMA 관찰로부터 나트륨화합물들이 누출부위 주변에 대량 침적되어 있는 것이 관찰되어 나트륨 철 크롬혼합물 형태로 부식생성물들이 혼재되어 있는 것으로 예측되었다.

  • PDF

청소년의 백제문화 유물에 대한 선호도와 패션문화상품 구매행동 연구 (A study on preference of Baekje culture relics and purchasing behaviors of fashion cultural products of adolescents)

  • 이미숙
    • 한국의상디자인학회지
    • /
    • 제20권4호
    • /
    • pp.41-56
    • /
    • 2018
  • The purposes of this study were to investigate preference of Baekje culture relics and to examine purchasing behaviors of fashion cultural products of adolescents. The subjects were 421 adolescents and measuring instruments consisted of Baekje relics preference items, fashion cultural products purchasing behaviors items, and subject' demographic attributions. The data were analyzed by frequency analysis, $x^2$ test, factor analysis, t-test, ANOVA and Duncan's multiple range test using SPSS program. The results were as follows. First, adolescents were more likely to prefer crown ornaments of the King Muryong in Kongju region, and gilt-bronze incense burner and halo in Buyeo region. Second, as fashion cultural products, adolescents were more likely to prefer T-shirts and accessory items, and modern image. As product selection criteria, they considered aesthetics as the most important factor, followed by symbolism and practicality. Adolescents evaluated the resonable purchase price of fashion cultural products as less than 20,000 won for T-shirts, cap & bag, and less than 10,000 won for accessories. The adolescent's satisfaction of fashion cultural products was low, and the main dissatisfaction was high prices, and the lack of practicality and diversity. Third, the preference for Baekje cultural relics and purchasing behaviors of fashion cultural products differed according to age and sex of adolescents. This study showed that fashion culture products for adolescents need to be designed in a modern sense with a unique cultural symbolism, focusing on T-shirts and accessories items. In addition, fashion cultural products companies should established the product development plan considering the characteristics according to the age and sex of adolescents.

안전모의 충격 흡수성 시험 개선에 관한 연구 (A Study on Improving Shock Absorption Test of Safety Helmet)

  • 심상우;심용수;이종빈;장성록
    • 한국안전학회지
    • /
    • 제38권5호
    • /
    • pp.36-42
    • /
    • 2023
  • In this study, 50 ABE-type hard hats were procured from five certified commercial manufacturers, and shock absorption tests were conducted in accordance with Protective Equipment Safety Certification Notice No. 2020-35. The tests were performed under both high- and low-temperature conditions, adhering to safety helmet testing standards. The highest shock transmission ranges were recorded in the tests, with an average energy range of 2,600-4,108 N at high temperatures and 2,316-3,991 N at low temperatures. All five hard hat models demonstrated a maximum transmitted impact force below 4,450 N, without any loss of cap and attachment functionality, confirming their compliance with performance standards. Furthermore, we evaluated the side impact performance of the safety helmets of each company, with an average range of 4,722-5,267 N. Company A exhibited the lowest measurement at 4,722 N. Comparing these results with international safety standards and the national shock absorption test criteria, it was observed that the maximum transmitted shock value using government-specified impact weight falls within the range of 4,450-5,000 N. However, it was noted that developed countries have established specific standards for the side impact forces on safety helmets, which are legally mandated. Consequently, it is imperative for South Korea to enhance its safety helmet side impact performance test methodology to align with domestic standards in the future.

키토산-은나노 녹색합성 복합물질을 적용한 항균 기능성 포장지 연구 (A Study of Antibacterial Paper Packaging Material Coated with Chitosan-Ag Nanocomposite Prepared by Green Synthesis)

  • 경규선;고성혁
    • 펄프종이기술
    • /
    • 제46권2호
    • /
    • pp.8-15
    • /
    • 2014
  • A novel antibacterial paper coated with chitosan-based silver (Ag) nanocomposite prepared by green synthesis has been investigated for a wide range of application in food, agricultural and medical packaging. Green synthesis of Ag nanoparticles (AgNPs) was carried out by a chemical reaction involving a mixture of chitosan-silver nitrate (AgNO3) in an autoclave at 15 psi, $121^{\circ}C$, for 15-120 sec. AgNPs and their formation in chitosan were confirmed by both UV-Vis spectroscopy and transmission electron microscope (TEM). Fourier transform infrared spectroscopy (FTIR) study showed that free amino groups in chitosan act as an effective reductant and AgNPs stabilizer. Antibacterial test of coated paper with as-prepared chitosan-AgNPs was performed qualitatively against E. coli based on the formation of halo zones around coated papers and it was shown to be effective in suppressing the growth of E. coli with increasing Ag contents in coating layer.

First Report of Pyrenophora tritici-repentis in Wheat (Triticum aestivum L.) in Korea and in vitro Selection of an Effective Fungicide

  • Min-Hye Jeong;Eu Ddeum Choi;Seol-Hwa Jang;Sang-Min Kim;Sook-Young Park
    • 한국균학회지
    • /
    • 제51권4호
    • /
    • pp.277-286
    • /
    • 2023
  • Tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease in wheat worldwide. In April 2021, tan spot symptoms were observed in a commercial wheat field in Suncheon, Jeonnam Province, Korea, with over 5% of the wheat leaves exhibiting symptoms. These symptoms included oval-shaped tan necrosis surrounded by a bright halo. The three representative isolates exhibited irregular mycelial growth on V8-potato dextrose agar and produced pseudothecia. Based on the concatenated sequence datasets of four multi-genes, including the internal transcribed spacer, large subunit ribosomal RNA, glyceraldehyde-3-phosphate dehydrogenase, and RNA polymerase II second-largest subunit genes, phylogenetic analysis revealed that these three isolates clustered in the same clade as P. tritici-repentis. Results of pathogenicity test indicated that the initial symptoms appeared 5 days post-inoculation (dpi), with typical tan spot symptoms developing at 7 dpi. The pathogen was successfully re-isolated from the symptomatic tissues, thus fulfilling Koch's postulates. Furthermore, we selected three fungicides that effectively inhibited the mycelial growth of P. tritici-repentis by more than 90% in vitro. To the best of our knowledge, this is the first report of tan spot disease in wheat in Korea.

동부(Vigna sinensis)의 국부병반을 이용한 Cucumber mosaic virus와 Broad bean wilt virus 2의 구별 (Discrimination of Cucumber mosaic virus and Broad bean wilt virus 2 Using Local Lesions on Vigna sinensis)

  • 배선화;김미순;정민영;권순배;류기현;김국형;최장경
    • 식물병연구
    • /
    • 제12권3호
    • /
    • pp.221-225
    • /
    • 2006
  • 용담에서 전형적인 모자이크 증상을 나타낸 바이러스 이병주 및 황화증상을 보이는 식물체를 채집하였다. 이들 중, 모자이크 증상의 잎을 접종하여 전신감염된 Nicotiana benthamiana로부터의 접종원을 동부의 초생엽에 접종하였을 때, 2가지 형태의 괴사 국부병반이 형성되었다. 즉 하나는 작은 크기 pinpoint 형태의 국부 괴사병반(SNS)이며, 다른 하나는 SNS보다 약간 큰 halo 형의 국부 괴사 병반(LNS)으로 뚜렷하게 구분되었다. 한편 황화 병징의 용담으로부터 증식된 접종원은 동부의 초생엽에 LNS만 형성하였다. 동부에 형성된 SNS 및 LNS형 병반을 각각 단일병반 분리하여 N. bentamiana에 접종하였다. 그 결과 SNS를 접종한 N. bentamiana에서는 접종엽에 무병징, 상위엽에는 전형적인 모자이크 증상이 발현되었다. 반면 LNS를 접종한 식물체에서는 접종엽에 퇴록 윤문이 형성되었으며, 상위엽에는 괴저를 동반한 모자이크 증상이 발현되었다. 이들 SNS 및 LNS 병반으로부터 분리한 바이러스를 dsRNA, RT-PCR 및 혈청학적 유연관계 분석을 통하여 SNS 병반은 CMV에 의해서, LNS는 BBWV2에 의해서 유도된다는 것을 확인하였다. 이 연구의 결과로부터 자연계에서 CMV와 BBWV2가 복합감염되어 병징의 구분이 어려운 경우, 동부에 접종하여 유도된 병반을 이용하여 두 바이러스의 감염을 쉽게 구분할 수 있는 방법으로 활용할 수 있을 것으로 생각되었다.

도재용착주조관용 Ni-Cr계 합금의 조성에 따른 표면특성 및 전단결합강도 관찰 (The Surface Property and Shear Bonding Strength according to Composition of Ni-Cr alloy for Porcelain Fused to Metal Crown)

  • 김갑진;정인성;최성민
    • 대한치과기공학회지
    • /
    • 제35권2호
    • /
    • pp.113-120
    • /
    • 2013
  • Purpose: This study was to observe surface property and bonding strength according to composition of Ni-Cr alloy for porcelain fused to metal crown. The two kinds of Ni-Cr alloy with different composition ratio of parent metal were observed general properties and chemical properties of each alloy surface and measured the shear bonding strength between ceramic and each alloys. The aim of study was to suggest the material for design of parent metal's composition ratio to development of alloy for porcelain fused to metal crown. Methods: The two kinds of alloy as test specimen was Ni(71wt.%)-Cr(12wt.%) and Ni(63wt.%)-Cr(23wt.%) alloy. The oxide on surface was observed by SEM and EDX. And the shear test was performed by MTS. Results: The surface property and oxide characteristic analysis of oxide layer, weight percentage of Element O within $Ni_{71}Cr_{12}$ alloy measured 12.74wt.%, but $Ni_{63}Cr_{23}$ alloy was measured 15.91wt.%. And the maximum shear bonding strength was measured 106.14MPa between $Ni_{71}Cr_{12}$ alloy and vintage halo (VV group). Conclusion: The surface property and oxide characteristic of $Ni_{71}Cr_{12}$ alloy was similar to $Ni_{63}Cr_{23}$ alloy. And VV group has the strongest shear bonding strength.