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http://dx.doi.org/10.5423/RPD.2006.12.3.221

Discrimination of Cucumber mosaic virus and Broad bean wilt virus 2 Using Local Lesions on Vigna sinensis  

Bae, Sun-Hwa (Department of Applied Biology, Kangwon National University)
Kim, Mi-Soon (Department of Applied Biology, Kangwon National University)
Jung, Min-Young (Department of Applied Biology, Kangwon National University)
Kwon, Soon-Bae (Gangwondo Agricultural Research and Extension Services)
Ryu, Ki-Hyun (Division of Environment & Life Sciences, Seoul Women's University)
Kim, Kook-Hyung (School of Agricultural Biotechnology, Seoul National University,)
Choi, Jang-Kyung (Department of Applied Biology, Kangwon National University)
Publication Information
Research in Plant Disease / v.12, no.3, 2006 , pp. 221-225 More about this Journal
Abstract
Cucumber mosaic virus(CMV) and Broad bean wilt virus 2(BBWV2) were isolated from Gentiana scabra plants showing typical mosaic and yellowing symptoms. When the inoculum of mosaic symptom propagated in Nicotiana benthamiana was inoculated to primary leaves of Vigna sinensis, the local lesions of different types was developed. Type one produced a small necrotic spot(SNS) of pinpoint type, while the other one showed a large necrotic spot(LNS) of halo type. LNS on primary leaves of V. sinensis was also induced by inoculum from yellowing symptom on G scabra. Single lesion from SNS induced a typical mosaic symptom on N. Benthamiana. On the other hand, LNS produced a chlorotic ring symptom on inoculated leaves and mosaic plus necrotic ringspot on upper leaves of N. benthamiana. An isolate of CMV from SNS and BBWV2 from LNS were detected by using dsRNA analysis, RT-PCR and agar gel double-diffusion test. Thus, our results should provide a tool of a simple method for discrimination from mixed infected plants by CMV and BBWV2.
Keywords
Cucumber mosaic virus; Broad bean wilt virus 2; Local lesions; Vigna sinensis;
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Times Cited By KSCI : 1  (Citation Analysis)
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