• Title/Summary/Keyword: half cell

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High frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures

  • Oh, Myung-Jin;Na, Hye-Ryun;Choi, Hong-Keun;Liu, Jang Ryol;Kim, Suk-Weon
    • Plant Biotechnology Reports
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    • v.4 no.2
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    • pp.125-128
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    • 2010
  • Culture conditions were established for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Nymphoides coreana. Zygotic embryos formed pale-yellow globular structures and calluses at a frequency of 85.6% when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. However, the frequency of pale-yellow globular structures and white callus formation decreased slightly with an increasing concentration of 2,4-D up to 10 $mg\;l^{-1}$ with the frequency rate falling to 16.7%. Cell suspension cultures were established from zygotic embryo-derived calluses using half-strength MS medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. Upon plating onto half-strength MS basal medium, over 92.3% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted into potting soil and achieved full growth to an adult plant in a growth chamber. The high frequency plant regeneration system for Nymphoides coreana established in this study will be useful for genetic manipulation and cryopreservation of this species.

Half and Full-Bridge Cell based Stand-Alone Photovoltaic Multi-Level Inverter (하프ㆍ풀-브리지 셀을 이용한 독립형 태양광 멀티레벨 인버터)

  • Kang Feel-Soon;Oh Seok-Kyu;Park Sung-Jun;Kim Jang-Mok;Kim Cheul-U
    • The Transactions of the Korean Institute of Power Electronics
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    • v.9 no.5
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    • pp.438-447
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    • 2004
  • A new multilevel PWM inverter using a half-bridge and full-bridge cells is proposed for the use of stand-alone photovoltaic inverters. The configuration of the proposed multilevel PWM inverter is based on a prior 11-level shaped PWM inverter. Among three full-bridge cells employed in the prior inverter, one cell is substituted by a half-bridge cell. Owing to this simple alteration, the proposed inverter has three promising merits. First it increases the number of output voltage levels resulted in high quality output voltages. Second, it reduces two power switching devices by means of employing a half-bridge cell. Third, it reduces power imposed on a transformer connected with the half-bridge unit. That is to say, most power is transferred to loads via cascaded transformers connected with low switching inverters, which are used to synthesize the fundamental output voltage levels whereas the output of a transformer linked to a high switching inverter is used to improve the final output voltage waves; thus, it is desirable in the point of the improvement of the system efficiency. By comparing to the prior 11-level PWM inverter, it assesses the performance of the proposed inverter as a stand-alone photovoltaic inverter. The validity of the proposed inverter is verified by computer-aided simulations and experimental results.

Alteration of 4-Aminopyridine-Sensitive, Voltage-Dependent $K^+-Channel$ in Arterial Smooth Muscle Cells of One-Kidney, One-Clip Goldblatt Hypertensive Rats

  • Kim, Hoe-Suk;Kim, Se-Hoon;Jeon, Byeong-Hwa;Chang, Seok-Jong
    • The Korean Journal of Physiology and Pharmacology
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    • v.4 no.5
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    • pp.385-391
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    • 2000
  • Using the patch-clamp technique, we investigated the alteration of 4-aminopyridine(4-AP)-sensitive, voltage-dependent $K^+$ channel (Kv) in the mesenteric arterial smooth muscle cell (MASMC) of renovascular hypertensive model, one-kidney one-clip Goldblatt hypertensive rat (GBH). To isolate $K_V$ current, internal pipette solution contained 5 mM ATP and 10 mM EGTA. Under these condition, MASMC was depolarized by 4-AP, but charybdotoxin did not affect membrane potential. Membrane potential of hypertensive cell $(-40.3{\pm}3.2\;mV)$ was reduced when compared to that of normotensive cell $(-59.5{\pm}2.8\;mV).$ Outward $K^+$ current of hypertensive cell was significantly reduced when compared to normotensive cell. At 60 mV, the outward currents were $19.10{\pm}1.91$ and $14.06{\pm}1.05$ pA/pF in normotensive cell and hypertensive cell respectively. 4-AP-sensitive $K^+$ current was also smaller in hypertensive cell $(4.28{\pm}0.38\;pA/pF)$ than in normotensive cell $(7.65{\pm}0.52\;pA/pF).$ The values of half activation voltage $(V_{1/2})$ and slope factor (k1) as well as the values of half inactivation voltage $(V_{1/2})$ and slope factor (k1) were virtually similar between GBH and NTR. These results suggest that the decrease of 4-AP-sensitive $K^+$ current contributes to a depolarization of membrane potential, which leads to development of vascular tone in GBH.

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A Study on the Development of Anode Material for Molten Carbonate Fuel Cell (용융탄산염 연료전지의 양극 대체재료의 개발에 관한 연구)

  • 황응림;김선지;강성군
    • Journal of Energy Engineering
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    • v.2 no.3
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    • pp.293-299
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    • 1993
  • In order to investigate the effect of Al addition on the electrochemical performance and structural stability of porous Ni anode for molten carbonate fuel cell, porous Ni anodes containing Al up to 10 wt% were fabricated by the tape casting technique. In this study half-cell performance of the anodes was evaluated by anodic polarization in the simulated MCFC anode condition(650$^{\circ}C$ , 80% H$_2$+20% CO$_2$). At the anodic current of 150 ㎃/$\textrm{cm}^2$, the polarizations for H$_2$oxidation of the anode was about 100 ㎷. The sintering and creep resistance of Ni-Al anodes was higher than those of the pure Ni anode. It was considered that the increase of sintering and creep resistance was due to the formation of Al$_2$O$_3$ on the surface of Ni particles.

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구강암의 조직비반세포에 관한 연구

  • Chun, Dong-Jin
    • The Journal of the Korean dental association
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    • v.12 no.1
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    • pp.21-28
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    • 1974
  • The author has observed the distribution of the tissue mast cells in 67 various tumors and precancerous lesions which occurred in the oral cavity. The specimcns ware obtained from the department of oral pathology, college of dentistry, Seoul National University, from Jan. 1970 to June, 1973. The results are as follows: 1) The number of the tissue mast cell was decrease predominantly in malignant tumors, especially in squamous cell carcinomas and in sarcomas. 2) The number of the tissue mast cell distirbution in adenocarcinomas one of malignant group was sligtly increased in with healthy oral mucosa. 3) The number of tissue mast cells in ameloblastomas one of benign group of the tumor of epithelial originwas more decreased than that in healthy oral mucosa. 4) The number of tissue mast cells in fibromas was more than that in healthy oral mucosa. 5) The number of the tissue mast cells in mixed tumors was increased one and a half times as many as that in healthy oral mucosa. 6) The number of the tissue mast cells in mixed tumors was increased one and a half times as many as that in healthy oral mucosa. 7) The tissue mast cell distribution can be observed more densly in the stroma of tumors than in the parenchyme of tumors.

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Evaluation of Durability of Slag Concrete by Marine Environment Exposure (해양환경 폭로에 의한 슬래그 콘크리트의 내구성 평가)

  • Kim, Hyun-Jin;Kim, Gyu-Yong;Choe, Gyeong-Cheol;Kim, Hong-Seop;Lee, Bo-Kyeong;Kim, Rae-Hwan
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2015.11a
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    • pp.111-112
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    • 2015
  • There is high possibility of steel corrosion on the reinforced concrete exposed to marine environment by chloride ion penetration. And it show a big difference of concrete durability under conditions of splash zone, tidal zone, and immersion zone. Therefore, in this paper, half-cell potential and chloride ion penetration depth was measured to evaluate the durability of slag concrete by marine exposure experiment. As a result, SC70 specimen showed no steel corrosion, regardless of the marine exposed conditions. Also, a deterrent effect on chloride ion penetration by replacement of slag in tidal zone and immersion zone could be confirmed.

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An Experimental Study on the Corrosion Monitoring of Reinforcing Steel in Concrete by the Accelerated Corrosion Test (부식촉진시험에 의한 콘크리트 내의 철근의 부식 모니터링에 관한 실험적 연구)

  • 배수호;정영수;김진영
    • Proceedings of the Korea Concrete Institute Conference
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    • 2001.11a
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    • pp.543-548
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    • 2001
  • The corrosion monitoring methods of reinforcing steel in concrete are the various methods such as half cell potential method, galvanic current method, resistivity method, polarization resistance method, AC impedance method and etc. In this study, the corrosion monitoring methods of reinforcing steel in concrete were investigated for the test specimens using corrosion inhibitors, zinc-mortar, zinc-plate, respectively. For this purpose, the accelerated corrosion tests for reinforcing steel were conducted according to the periodic cycles(140 days) of wetting($65^{\circ}C$, 90% R.H.) and drying period(15$^{\circ}C$ , 65% R.H.) for the test specimens. As a result, it can be concluded from the test that half cell potential and galvanic current method as monitoring techniques for corrosion were found to be relatively reliable and easily usable method in the field.

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An Experimental Study on the Reinforcement Corrosion in Concrete Using Granulated Blast Furnace Slag (고로슬래그 미분말을 사용한 콘크리트 내의 철근부식에 관한 실험적 연구)

  • 이동혁;김은겸;김영웅;김영철
    • Proceedings of the Korea Concrete Institute Conference
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    • 2002.05a
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    • pp.761-766
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    • 2002
  • This paper was peformed to verify the effect that granulated blast furnace slag gets in reinforcement corrosion resistance about chloride ion that invade from outside. An experiment accelerated for the reinforcement corrosion through repeat of brine digestion and dry. Reinforcement corrosion investigated half cell potential method of measurement by ASTM C876 and corrosion area ratio. If granulated blast furnace slag metathesis ratio is high generally that looked the corrosion of reinforcement decreasing as a result that evaluate reinforcement corrosion by ASTM C876 canon in this research. It showed high resistance about reinforcement corrosion that use normal portland cement and increase the metathesis rate of granulated blast furnace slag, as a result that evaluate metathesis rate effect of granulated blast furnace slag according to cement kind. when the test piece split destroying, area rate of reinforcement corrosion showed about result of half cell potential measurement.

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A New Single-Stage PFC AC/DC Converter with Low Link-Capacitor Voltage

  • Lee, Byoung-Hee;Kim, Chong-Eun;Park, Ki-Bum;Moon, Gun-Woo
    • Journal of Power Electronics
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    • v.7 no.4
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    • pp.328-335
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    • 2007
  • A conventional Single-Stage Power-Factor-Correction (PFC) AC/DC converter has a link capacitor voltage problem under high line input and low load conditions. In this paper, this problem is analyzed by using the voltage conversion ratio of the DC/DC conversion cell. By applying this analysis, a new Single-Stage PFC AC/DC converter with a boost PFC cell integrated with a Voltage-Doubler Rectified Asymmetrical Half-Bridge (VDRAHB) is proposed. The proposed converter features good power factor correction, low current harmonic distortions, tight output regulations and low voltage of the link capacitor. An 85W prototype was implemented to show that it meets harmonic requirements and standards satisfactorily with near unity power factor and high efficiency over universal input.