• 제목/요약/키워드: half cell

검색결과 824건 처리시간 0.029초

High frequency plant regeneration system for Nymphoides coreana via somatic embryogenesis from zygotic embryo-derived embryogenic cell suspension cultures

  • Oh, Myung-Jin;Na, Hye-Ryun;Choi, Hong-Keun;Liu, Jang Ryol;Kim, Suk-Weon
    • Plant Biotechnology Reports
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    • 제4권2호
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    • pp.125-128
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    • 2010
  • Culture conditions were established for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Nymphoides coreana. Zygotic embryos formed pale-yellow globular structures and calluses at a frequency of 85.6% when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. However, the frequency of pale-yellow globular structures and white callus formation decreased slightly with an increasing concentration of 2,4-D up to 10 $mg\;l^{-1}$ with the frequency rate falling to 16.7%. Cell suspension cultures were established from zygotic embryo-derived calluses using half-strength MS medium supplemented with 0.3 $mg\;l^{-1}$ of 2,4-D. Upon plating onto half-strength MS basal medium, over 92.3% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted into potting soil and achieved full growth to an adult plant in a growth chamber. The high frequency plant regeneration system for Nymphoides coreana established in this study will be useful for genetic manipulation and cryopreservation of this species.

하프ㆍ풀-브리지 셀을 이용한 독립형 태양광 멀티레벨 인버터 (Half and Full-Bridge Cell based Stand-Alone Photovoltaic Multi-Level Inverter)

  • 강필순;오석규;박성준;김장목;김철우
    • 전력전자학회논문지
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    • 제9권5호
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    • pp.438-447
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    • 2004
  • 본 논문에서는 하프ㆍ풀-브리지 셀을 이용하는 새로운 멀티레벨 인버터를 제안하고 독립형 태양광 인버터로서의 가능성을 평가한다. 기본 회로 구조는 기존의 11-레벨형 PWM 인버터에 기초하며 세 개의 풀-브리지 모듈 중 하나의 모듈을 하프-브리지 구조로 대체한다. 이 간단한 회로 구조의 변경으로 제안된 멀티레벨 인버터는 세 가지의 장점을 가지게 된다. 첫째, 출력 전압 레벨 수의 증가를 통해 보다 정현적인 출력 전압 파형을 형성 시킬 수 있다. 둘째, 풀-브리지 모듈 대신 하프-브리지 셀을 이용하므로 두 개의 전력 스위치를 줄일 수 있다. 셋째, 쵸핑용으로 이용되는 인버터와 결합된 변압기에 의해 부하로 전달되는 전력량을 저감 시킬 수 있어 전체 효율 개선에 유리하다. 즉, 대부분의 전력은 저주파 스위칭 동작으로 기본 출력전압을 형성하는 인버터와 이와 결합된 변압기에 의해 부하단으로 전달되고, 고주파 스위칭을 하는 쵸핑용 인버터와 이와 결합된 변압기를 통한 출력은 최종 출력전압을 보다 정현화 하기 위한 목적으로 사용되도록 한다. 기존의 11-레벨형 PWM 인버터와 비교를 통해 제안하는 인버터를 평가하고, 컴퓨터 시뮬레이션 및 실험 결과를 통해 독립형 태양광 인버터로서의 가능성을 입증한다.

Alteration of 4-Aminopyridine-Sensitive, Voltage-Dependent $K^+-Channel$ in Arterial Smooth Muscle Cells of One-Kidney, One-Clip Goldblatt Hypertensive Rats

  • Kim, Hoe-Suk;Kim, Se-Hoon;Jeon, Byeong-Hwa;Chang, Seok-Jong
    • The Korean Journal of Physiology and Pharmacology
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    • 제4권5호
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    • pp.385-391
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    • 2000
  • Using the patch-clamp technique, we investigated the alteration of 4-aminopyridine(4-AP)-sensitive, voltage-dependent $K^+$ channel (Kv) in the mesenteric arterial smooth muscle cell (MASMC) of renovascular hypertensive model, one-kidney one-clip Goldblatt hypertensive rat (GBH). To isolate $K_V$ current, internal pipette solution contained 5 mM ATP and 10 mM EGTA. Under these condition, MASMC was depolarized by 4-AP, but charybdotoxin did not affect membrane potential. Membrane potential of hypertensive cell $(-40.3{\pm}3.2\;mV)$ was reduced when compared to that of normotensive cell $(-59.5{\pm}2.8\;mV).$ Outward $K^+$ current of hypertensive cell was significantly reduced when compared to normotensive cell. At 60 mV, the outward currents were $19.10{\pm}1.91$ and $14.06{\pm}1.05$ pA/pF in normotensive cell and hypertensive cell respectively. 4-AP-sensitive $K^+$ current was also smaller in hypertensive cell $(4.28{\pm}0.38\;pA/pF)$ than in normotensive cell $(7.65{\pm}0.52\;pA/pF).$ The values of half activation voltage $(V_{1/2})$ and slope factor (k1) as well as the values of half inactivation voltage $(V_{1/2})$ and slope factor (k1) were virtually similar between GBH and NTR. These results suggest that the decrease of 4-AP-sensitive $K^+$ current contributes to a depolarization of membrane potential, which leads to development of vascular tone in GBH.

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용융탄산염 연료전지의 양극 대체재료의 개발에 관한 연구 (A Study on the Development of Anode Material for Molten Carbonate Fuel Cell)

  • 황응림;김선지;강성군
    • 에너지공학
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    • 제2권3호
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    • pp.293-299
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    • 1993
  • 용융탄산염연료전지(MCFC)용 다공성 Ni 양극에 3~10 wt% Al를 첨가하여 tape casting 법으로 제조된 Ni-Al 양극의 전기화학적성능 및 구조적안정성이 조사되었다. 본 연구에서 제조된 양극의 전기화학적 성능이 $650^{\circ}C$, MCFC 양극분위기(80% H$_2$+20% $CO_2$)를 모사한 half-cell 에서 양분극 특성으로 평가되었는데, 전류밀도 150 ㎃/$\textrm{cm}^2$ 에서의 분극전압은 약 100 ㎷로 실용전지의 양극으로서 가능성을 보였다. Ni-Al 양극의 소결과 creep에 대한 저항성은 Ni 양극에 비해 증가되었는데, 이는 Ni 입자 표면에 형성된 $Al_2$O$_3$의 영향으로 판단되었다.

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구강암의 조직비반세포에 관한 연구

  • 전동진
    • 대한치과의사협회지
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    • 제12권1호
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    • pp.21-28
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    • 1974
  • The author has observed the distribution of the tissue mast cells in 67 various tumors and precancerous lesions which occurred in the oral cavity. The specimcns ware obtained from the department of oral pathology, college of dentistry, Seoul National University, from Jan. 1970 to June, 1973. The results are as follows: 1) The number of the tissue mast cell was decrease predominantly in malignant tumors, especially in squamous cell carcinomas and in sarcomas. 2) The number of the tissue mast cell distirbution in adenocarcinomas one of malignant group was sligtly increased in with healthy oral mucosa. 3) The number of tissue mast cells in ameloblastomas one of benign group of the tumor of epithelial originwas more decreased than that in healthy oral mucosa. 4) The number of tissue mast cells in fibromas was more than that in healthy oral mucosa. 5) The number of the tissue mast cells in mixed tumors was increased one and a half times as many as that in healthy oral mucosa. 6) The number of the tissue mast cells in mixed tumors was increased one and a half times as many as that in healthy oral mucosa. 7) The tissue mast cell distribution can be observed more densly in the stroma of tumors than in the parenchyme of tumors.

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해양환경 폭로에 의한 슬래그 콘크리트의 내구성 평가 (Evaluation of Durability of Slag Concrete by Marine Environment Exposure)

  • 김현진;김규용;최경철;김홍섭;이보경;김래환
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2015년도 추계 학술논문 발표대회
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    • pp.111-112
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    • 2015
  • There is high possibility of steel corrosion on the reinforced concrete exposed to marine environment by chloride ion penetration. And it show a big difference of concrete durability under conditions of splash zone, tidal zone, and immersion zone. Therefore, in this paper, half-cell potential and chloride ion penetration depth was measured to evaluate the durability of slag concrete by marine exposure experiment. As a result, SC70 specimen showed no steel corrosion, regardless of the marine exposed conditions. Also, a deterrent effect on chloride ion penetration by replacement of slag in tidal zone and immersion zone could be confirmed.

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부식촉진시험에 의한 콘크리트 내의 철근의 부식 모니터링에 관한 실험적 연구 (An Experimental Study on the Corrosion Monitoring of Reinforcing Steel in Concrete by the Accelerated Corrosion Test)

  • 배수호;정영수;김진영
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2001년도 가을 학술발표회 논문집
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    • pp.543-548
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    • 2001
  • The corrosion monitoring methods of reinforcing steel in concrete are the various methods such as half cell potential method, galvanic current method, resistivity method, polarization resistance method, AC impedance method and etc. In this study, the corrosion monitoring methods of reinforcing steel in concrete were investigated for the test specimens using corrosion inhibitors, zinc-mortar, zinc-plate, respectively. For this purpose, the accelerated corrosion tests for reinforcing steel were conducted according to the periodic cycles(140 days) of wetting($65^{\circ}C$, 90% R.H.) and drying period(15$^{\circ}C$ , 65% R.H.) for the test specimens. As a result, it can be concluded from the test that half cell potential and galvanic current method as monitoring techniques for corrosion were found to be relatively reliable and easily usable method in the field.

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고로슬래그 미분말을 사용한 콘크리트 내의 철근부식에 관한 실험적 연구 (An Experimental Study on the Reinforcement Corrosion in Concrete Using Granulated Blast Furnace Slag)

  • 이동혁;김은겸;김영웅;김영철
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2002년도 봄 학술발표회 논문집
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    • pp.761-766
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    • 2002
  • This paper was peformed to verify the effect that granulated blast furnace slag gets in reinforcement corrosion resistance about chloride ion that invade from outside. An experiment accelerated for the reinforcement corrosion through repeat of brine digestion and dry. Reinforcement corrosion investigated half cell potential method of measurement by ASTM C876 and corrosion area ratio. If granulated blast furnace slag metathesis ratio is high generally that looked the corrosion of reinforcement decreasing as a result that evaluate reinforcement corrosion by ASTM C876 canon in this research. It showed high resistance about reinforcement corrosion that use normal portland cement and increase the metathesis rate of granulated blast furnace slag, as a result that evaluate metathesis rate effect of granulated blast furnace slag according to cement kind. when the test piece split destroying, area rate of reinforcement corrosion showed about result of half cell potential measurement.

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A New Single-Stage PFC AC/DC Converter with Low Link-Capacitor Voltage

  • Lee, Byoung-Hee;Kim, Chong-Eun;Park, Ki-Bum;Moon, Gun-Woo
    • Journal of Power Electronics
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    • 제7권4호
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    • pp.328-335
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    • 2007
  • A conventional Single-Stage Power-Factor-Correction (PFC) AC/DC converter has a link capacitor voltage problem under high line input and low load conditions. In this paper, this problem is analyzed by using the voltage conversion ratio of the DC/DC conversion cell. By applying this analysis, a new Single-Stage PFC AC/DC converter with a boost PFC cell integrated with a Voltage-Doubler Rectified Asymmetrical Half-Bridge (VDRAHB) is proposed. The proposed converter features good power factor correction, low current harmonic distortions, tight output regulations and low voltage of the link capacitor. An 85W prototype was implemented to show that it meets harmonic requirements and standards satisfactorily with near unity power factor and high efficiency over universal input.