• Journal of Microbiology and Biotechnology
• /
• 제7권5호
• /
• pp.293-298
• /
• 1997

A 4.6-kb HindIII fragment encompassing the complete 140-kDa ${\alpha}$-amylase gene of Lactobacillus amylovorus B 4540 was cloned into pBR322 by the shot gun method. Southern blotting and restriction mapping for the insert were performed. The recombinant 9.0-kb plasmid, pFML1, conferred ${\alpha}$-amylase activity to E. coli and Lactococcus lactis hosts when introduced by electroporation. SDS-PAGE and zymography confirmed the production of 140-kDa ${\alpha}$-amylase and its proteolytic degradation products with enzyme activity in transformants. Total ${\alpha}$-amylase activity of E. coli $DH5{\alpha}$ cells harboring pFML1 was 1.8 units and most activity was detected from cell pellets. Total enzyme activity of L. lactis subsp. lactis MG1363 transformant was five to ten-fold lower than that of E. coli cell but more than half of the activity was detected in the culture supernatant.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권4호
• /
• pp.2201-2205
• /
• 2013

Colorectal cancer (CRC) is one of the most common cancers in many parts of the world. Its development is a multi-step process involving three distinct stages, initiation that alters the molecular message of a normal cell, followed by promotion and progression that ultimately generates a phenotypically altered transformed malignant cell. Reports have suggested an association of the phosphoinositide-3-kinase (PI3K)/Akt pathway with colon tumorigenesis. Activation of Akt signaling and impaired expression of phosphatase and tensin homolog (PTEN) (a negative regulator of Akt) has been reported in 60-70% of human colon cancers and inhibitors of PI3K/Akt signaling have been suggested as potential therapeutic agents. Around 80% of human colon tumors possess mutations in the APC gene and half of the remainder feature ${\beta}$-catenin gene mutations which affect downstream signaling of the PI3K/Akt pathway. In recent years, there has been a great focus in targeting these signaling pathways, with natural and synthetic drugs reducing the tumor burden in different experiment models. In this review we survey the role of PI3K/Akt/mTOR and Wnt signaling in CRC.

• 혜화의학회지
• /
• 제28권2호
• /
• pp.1-11
• /
• 2019

Objective: The aim of this study is to analyze the previously published research articles related to puffer fish toxin focusing on tumor. Method: Literatures were searched in PubMed database, published since 2000, using the keyword; Puffer fish, Fugu and tetrodotoxin (TTX) with cancer or tumor. Research papers were classified by year, country, study model, used material, kind of tumor and study subject. Finally, a total of 41 studies were analyzed in this study. Results: From 2000 to 2018, the most abundant papers were published in 2009 (6 studies) and almost half of the papers were studied in United Kingdom (20 studies). The 39 studies used TTX purified from puffer fish while 2 studies used crude extract of skin and gonad of puffer fish. The most used target cell line was prostate cancer (15 studies), and the next was breast cancer (14 studies). The study methods were classified into 4 clinical studies, 2 animal studies and 35 cell-based studies. Conclusions: Our results show that the overview of cancer-related studies using puffer fish poison. This information would be helpful for the puffer fish-derived drug researches in the future.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제10권4호
• /
• pp.316-324
• /
• 2010

In this paper, we design a 256 kbits EEPROM for a MCU (Microcontroller unit) with the wide voltage range of 1.8 V to 5.5 V. The memory space of the EEPROM is separated into a program and data region. An option memory region is added for storing user IDs, serial numbers and so forth. By making HPWs (High-voltage P-wells) of EEPROM cell arrays with the same bias voltages in accordance with the operation modes shared in a double word unit, we can reduce the HPW-to-HPW space by a half and hence the area of the EEPROM cell arrays by 9.1 percent. Also, we propose a page buffer circuit reducing a test time, and a write-verify-read mode securing a reliability of the EEPROM. Furthermore, we propose a DC-DC converter that can be applied to a MCU with the wide voltage range. Finally, we come up with a method of obtaining the oscillation period of a charge pump. The layout size of the designed 256 kbits EEPROM IP with MagnaChip's 0.18 ${\mu}m$ EEPROM process is $1581.55{\mu}m{\times}792.00{\mu}m$.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2008년도 춘계학술대회 및 기술 세미나 논문집 디스플레이 광소자
• /
• pp.51-52
• /
• 2008

A single gap transflective liquid crystal display(LCD) driven by fringe electric field in the transmissive(T) region and vertical electric field in the reflective(R) region was designed. Because the pretilt angle is formed in $53^{\circ}$ through polymerization of an UV curable reactive mesogen(RM) monomer at the surface, the effective cell retardation $(d{\Delta}n_{eff})$ value of the R region becomes half of that in the T region where the LCs are homogenously aligned. Consequently, a transflective display driven by a vertical and fringe electric field with a single cell gap and single gamma curves is realized.

• 한국태양광발전학회지
• /
• 제6권2호
• /
• pp.15-24
• /
• 2020

영농형 태양광 발전은 농업과 태양광 발전 시스템의 동시 상생이 가능한 기술로 현재와 같은 농업 환경 및 생태계 개선 그리고 에너지 전환의 시대에 대처해야 하는 훌륭한 대안이다. 한국형 영농형 태양광 발전 시스템 개발 및 보급을 위한 국내 첫 연구개발 사업인 농림축산식품부(농림식품기술기획평가원 첨단생산기술개발사업)의 "한국형 태양광 이모작(농업&태양광 발전 병행) 스마트 영농 시스템 개발(과제번호: 1545015977)"과제를 통해 총 60kW급 규모의 5개소 실증단지(10kW급 3개소 신규 개발 및 구축)를 활용한 최적 시스템 개발, 6품종(벼 2품종, 감자, 배추, 마늘, 양파 및 배) 표준재배기술 도출 등을 수행하였다. 기존 태양광 발전용 모듈(72cell) 대비 half-size(36cell) 모듈 개발, 작물 별 최적 구조물(차광률 30% 미만 적용) 도출, 대상 작물 감수율 20% 미만 달성 및 표준재배기술개발을 수행하였다. 추후 장기간 동안 영농형 태양광 구조물의 경제성 확보, 시공성 개선 그리고 다양한 작물 대상 표준재배기술 최적화 등의 실증연구가 필요하다. 마지막으로 영농형 태양광 발전 시스템의 확산 및 보급을 위해 국가적 지원과 농민들의 적극적인 참여 없이는 불가능 하다. 그리고 본 개발 기술은 농업인을 위한 기술로 범국민적인 수용성 개선 또한 수반되어야 한다. 특히, 정부는 영농형 태양광 발전 시스템이 대한민국의 식량안보와 에너지안보를 확보를 위해 농업진흥지역에 대한 최대 20년까지 타용도 일시전용이 가능하도록 농지법 개정으로 화답 할 때이다.

• 한국미생물·생명공학회지
• /
• 제25권4호
• /
• pp.427-433
• /
• 1997

Sludge Returned CMAS process was applied to treat the wastewater from electric accessory manufacturing company while this type of wastewater was usually treated by chemical process. This result show that the removal rate of TCOD was about 70-80% regardless of hydraulic retention time, On the contrary, the removal rate of BOD was abtained in a range of 77-92% depending on hydraulic retention time. In order to remove more than 80% of organic materials with the proposed process, the F/M ratio should be maintained below 0.17. In this case, the calculated value of organic removal rate, Km, was calculated to be 1.26 hr$^{-1}$, and the ratio of cell synthesis/total energy was 0.32 and 0.26 for COD and BOD base, respectively. The yield coefficient was calculated to be 0.242 and the half velocity coefficient was 0.3 hr$^{-1}$. The value of endogenous respiration coefficient was 0.02 hr$^{-1}$. The measured effluent BOD concentration, MLSS concentration in aeration tank, oxygen uptake rate, and sludge production were matched relatively well with the calculated values using above coefficients, In order to optimize the dewatering of sludge, the hydraulic retention time was recommended to be 15. 6 hrs. These results indicate that the wastewater from an eletric accessory manufacturing company can be treated safely with a biological process.

• Journal of Microbiology and Biotechnology
• /
• 제5권5호
• /
• pp.285-291
• /
• 1995

Saccharide utilizations for the growth by Bifidobacterium longum and Bifidobacterium breve were compared. B. longum fermented glucose more rapidly than lactose as a carbon source whereas B. breve fermented lactose at a rate higher than that of glucose. The highest cell concentration, in the case of B. longum, was obtained when cultivated in a jar fermentor that contained modified MRS medium that half the beef extract was replaced by the same amount of tuna extract, and that pH was controlled at 6.0. B. breve showed the best growth when grown in a jar fermentor containing the MRS medium with lactose instead of glucose, controlled at pH 6.0. The optimal concentration of peptone in MRS medium for the growth of B. breve was 5 g/l.

• Plant Resources
• /
• 제5권2호
• /
• pp.141-154
• /
• 2002

In this experiment, three kinds of mutations(ge, re, and eml )relating to the size of embryos were used to study their generation, genetic mechanism and developmental characteristics, and the interactions between embryo and endosperm were also examined. Giant embryo mutation comprises 7 kinds including the already isolated ge, and ge-2, which share an identical gene site. The SAM and the size of radicule for the ge showed little difference compared to a normal type. The number of embryo cells did not increased as much as it would affect the size of embryo. Therefore, the enlargement of embryo was due to the enlargement of scutellum that originated from the corpulence of each cell. Both F$_1$' s of re ]and odm 49 formed reduce embryos, and other combinations of hybridization showed all wild type of embryo sizes. Accordingly, the odm 49 must have an identical gene site of re 1, while odm 48 and odm 62 have different gene sites. Their shoots and radicules also shrank by the same ratio, however no sign of physical change was noticed. The size of embryo cell showed no change, while the number of cells was the half of that of wild types. The three gene sites of re represent all of them control the size of the entire embryo forming organs. The eml 1 was defined to have temperature sensibilities that the generation of endosperms was active at a high temperature while that was hampered at a low temperature.

• 식물조직배양학회지
• /
• 제21권3호
• /
• pp.183-186
• /
• 1994

태백벼의 현탁배양세포주로부터 유도된 체세포배를 낱개로 알진산 캡슐화하여 인공종자화하였다. 인공종자는 1/2 MS 고체배지에서 73%의 발아율을 나타내었으며 알진산 캡슐은 체세포배의 발아율에 영향을 주지 않았다. 그러나 멸균되지 않은 여과지에서는 발아율이 60%로 낮아졌다. 캡슐화된 진정종자는 무균상태여부에 관계없이 높은 발아율을 나타내었다. 이상의 결과로 무균상태가 유지되지 않을 때 인공종자 발아율이 낮아지는 것은 체세포배가 진정종자의 접합자배보다 오염을 견디기 어렵기 때문인 것으로 사료된다.