• 제목/요약/키워드: growth-line

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세포증식과 증식속도의 On-line Monitoring을 위한 Computer- coupled Mass Spectrometer의 응용 (Application of Computer-coupled Mass Spectrometer for Continuous On-line Monitoring of Cell Growth and Growth Rate)

  • 남수완;최춘순;김정회
    • 한국미생물·생명공학회지
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    • 제17권3호
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    • pp.241-246
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    • 1989
  • Quadrupole mass spectrometer를 이용한 발효 배기가스의 분석을 통해 세포의 증식을 on-line monitoring하고자 model 균주로 Candida utilis에 대해 연구하였다. Quadrupole mass spectrometer와 interface된 16-bit 개인용 컴퓨터 (IBM PC-AT)에서 산소 소비속도(OUR)와 이산화탄소 발생속도(CER)를 on-line 계산할 수 있었고 계속해서 이들 계산치로부터 세포농도와 증식속도 및 비증식속도를 계산하였다. 계산된 값들은 실험적으로 측정한 세포농도 및 비증식속도와 잘 일치함을 알 수 있었다.

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Diphasic Analysis of Growth in Japanese Quail

  • Ozkan, Muhip
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권9호
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    • pp.1281-1285
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    • 2004
  • A line of Japanese quail selected for increased body weight for 15 generations (C) and an unselected control line (K) were used to examine the impact of selection for body weight on the growth curve of Japanese quail. In addition, the effect of sex on the growth curve in each line was also studied, namely females of C (CF), males of C (CM), females of K (KF) and males of K (KM). The monophasic and diphasic growth models were studied for adequacy in describing growth curves of quail in both sexes of the C and K lines. The monophasic function provided almost the same growth rate for both sexes in both lines. However, the growth rates calculated by means of the diphasic function differed between sexes for both lines, except for those calculated for C during the second growth phase. While there were 2-3 days difference between sexes in age at maximum gain in both lines with a monophasic model, the difference between sexes in the age at maximum gain in both lines became greater according to the diphasic model. There were 5 and 7 days difference between sexes in the age at maximum gain in line C for the first and second growth phases, respectively. A difference between sexes of 18 and 11 days in the age at maximum gain for the first and second phases, respectively, was estimated for line K when the diphasic function was fitted. The use of diphasic functions provides more detailed information on growth patterns. The results showed that the use of the diphasic function was better because it provided greater insights into understanding the biology of growth.

식물의 경계분포 이론과 백두산 삼림한계량의 파동성 (The Theory of Boundary Distribution of the Plant and Wave Character of the Timber Line on Mt. Paektu)

  • 장남기;심규철;이현욱;강경미;소금현
    • The Korean Journal of Ecology
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    • 제21권5_2호
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    • pp.491-499
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    • 1998
  • An investigation was performed to establish the mathematical theories of a vibration for the plant growth and a wave distribution of a plant population on the boundary condition of a limiting factor in the environment. The mathematical theories of the plant growth vibration and wave distribution had been elucidated by the plant growth and the timber line on the middle slope of the west side of Mt. Paektu. The Betula ermaruii composes the timber line on about 2,060 m elevation of sea label, has a growth vibration on the ground surface and takes a wave distribution due to a boundary condition of alpine temperature gradient.

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Growth Performance of Offspring from Selected and Non-Selected Brood Line of Red Sea Bream, Pagrus major

  • Noh, Choong-Hwan;Hong, Kyung-Pyo;Myoung, Jung-Goo;Kim, Jong-Man
    • 한국양식학회:학술대회논문집
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    • 한국양식학회 2003년도 추계학술발표대회 논문요약집
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    • pp.36-36
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    • 2003
  • In the present study, growth performances of the offspring from selected brood line were compared to those of the offspring from non-selected brood line of red sea bream. Offspring groups were mass produced separately from two brood lines, selected and non-selected Korean strain. Selected brood line have been selected by fish size for four generations (upper 5∼30% per generation) and non-selected brood line is the second generation of wild population at south sea in Korea. There's no significant difference in body length between offspring from selected and non-selected brood line during early growing stage (until 96-days old). However, offspring from selected brood line had superior body weight growth than offspring from non-selected brood line. At sea cages rearing trials with communal stocking, Offspring from selected brood line showed significantly better performance in body weight, body length, weight gain, specific growth rate and feed consumption (but not in feed conversion ratio) than offspring from non-selected brood line. At 24 months old, offspring from selected brood line grew faster 1.10 times in body length and 1.41 times in body weight than offspring from non selected brood line. The response to selection when compared to a non-selected line is on average of 10% in weight per generation at 24 months old.

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Hormonal Regulation of Insulin-Like Growth Factor Binding Protein Secretion by a Bovine Mammary Epithelial Cell Line

  • Kim, W.Y.;Chow, J.C.;Hanigan, M.D.;Calvert, C.C.;Ha, J.K.;Baldwin, R.L.
    • Asian-Australasian Journal of Animal Sciences
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    • 제10권2호
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    • pp.233-239
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    • 1997
  • A mammary epithelial cell line (MAC-T) established as a model for lactation was utilized to identify and characterize effects of various hormones upon insulin-like growth factor binding protein secretion. Ligand and immunoblot analyses of conditioned media indicated that insulin-like growth factor binding protein-2 was secreted by MAC-T cells. Insulin-like growth factor-I stimulated insulin-like growth factor binding protein-2 secretion in a dose-dependent manner, but prolactin and bovine somatotropin did not alter insulin-like growth factor binding protein-2 secretion. Insulin increased and cortisol decreased insulin-like growth factor binding protein-2 secretion. Effects of insulin-like growth factor-I on insulin-like growth factor binding protein-2 secretion support previous studies using primary cultures of bovine mammary cells and bovine fibroblasts. Effects of cortisol and insulin on insulin-like growth factor binding protein-2 secretion may be explained by changes in protein synthesis. In addition, supraphysiological doses of insulin can cross-react with the insulin-like growth factor-I receptor and stimulate insulin-like growth factor binding protein-2 secretion. MAC-T cells provide a model system to study mechanisms that regulate local insulin-like growth factor-I bioactivity.

선발과 비선발 참돔 (Pagrus maior) 계통의 성장 양상 비교 (Comparative Growth Performance of the Selected and the Non-selected Red Sea Bream (Pagrus major) Lines)

  • 노충환;홍경표;오승용;최희정;박용주;명정구;김종만
    • 한국수산과학회지
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    • 제37권5호
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    • pp.400-404
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    • 2004
  • Growth performances of the selected and the non-selected red sea bream (Pagrus major) lines were evaluated up to marketable size. The offspring were mass-produced either from the selected line (the fourth generation of broods from mass selection) or the non-selected line (the second generation of wild broods). Early (59 to 103 days old, trial I) and late growth performances (4 to 24 months old, trial II) were evaluated based on communal rearing in land-based tanks and sea cages, respectively. In trial I, the selected line exhibited significantly higher values in both weight gain (WG) and specific growth rate (SGR) when compared to those observed in the non-selected line (P<0.05). In trial II comprising of three growth phases (4-9, 9-16 and 16-24 months old), selected line showed only slightly improved in WG and SGR at the beginning two growth phases (4-9 and 9-16 months old) (P>0.05), however, displayed significantly higher values at the last growth phase (16-24 months old) (P<0.05). Overall WG and SGR throughout the experiment (4-24 months) were also significantly higher in the selected line than the non-selected line (P<0.05). Consequently, mean body weight of the selected line at 24 months old was 1.4-fold (40.8{\%}\;increased) as compared to that of the non-selected line. This present result indicates that mass selection of this species against the growth traits might be quite effective, and the present advance has important Implication in the productivity enhancement of red sea bream aquaculture.

Cytodex-3를 이용한 부착성 동물세포의 미립담체 배양 (Microcarrier Culture of an Anchorage-dependent Cell Using Cytodex-3)

  • 김정회;최준호;웨이슈후
    • 한국미생물·생명공학회지
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    • 제17권3호
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    • pp.231-235
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    • 1989
  • 부착성 동물세포인 recombinant human embryonic kidney cell line 293을 5% FBS가 함유된 DME/F12(3:1) mixture 배지에서 배양할 경우 static culture에서와는 달리 미립담체 배양에서는 낮은 부착효율과 spreading의 저해현상이 관찰되었다. Cell line 293의 미립담체 배양에 있어서 배지내의 serum은 세포의 미립담체에 대한 부착속도(attach-ment rate)를 감소시킬 분만 아니라 세포의 증식을 저해한다는 사실을 확인하였다. 이러한 경우, conditioned microcarrier를 이용함으로써 미립담체로부터 세포의 탈착을 어느 정도 막을 수 있었다. 특히 autocrine growth factor를 생성하는 hepatoma cell line인 HpG 2 cell을 배양하여 얻은 conditioned medium을 serum-free medium에 첨가하여 사용함으로써 세포의 정상적인 성장 분만 아니라 growth-stimulation을 유도할 수 있었다.

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조간대 저토 환경과 갈대의 생장 특성 (Sediment Properties and Growth of Phragmites australis in Mud Tidal Flat)

  • 민병미
    • 한국환경복원기술학회지
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    • 제14권3호
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    • pp.57-69
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    • 2011
  • This study examined the relationship between Phragmites australis' growth and sediment properties at mud tidal flat of Donggum-ri, Gilsang-myeon, Gangwha-gun, Incheon city. Field survey was carried out from May, 2010 to October, 2010. Water content, soil texture, electric conductivity and water table depth for sediment, density, height, dry weight and flowering for P. australis were examined at several plots from the starting point (the coastal embankment) to the end point of the two populations. The result was as follows. Firstly, the water table increased along distance from the embankment at one line (N-line) but was similar at the other line (S-line) in a P. asustralis population. Water tables were higher out of than within a P. australis population at two populations. Secondary, in N-line, the height and dry weight of P. australis decreased along the distance from embankment but, in S-line, those were similar in its population. P. australis' growth was dependent on electric conductivity at lower layer (water table level) rather than upper one (the surface). Thirdly, density of P. australis changed during growing season and was similar in a population, except for the end point of patch. In summary, the growth and distribution of P. australis were dependent on salt content of tidal flat's sediment (water table level) and this was affected by fresh water of the inland.

Development of a Live-line Insulator Inspection Tool System for 154 kV Power Transmission Lines

  • Lee, Jae-Kyung;Park, Joon-Young;Cho, Byung-Hak;Oh, Ki-Yong
    • Journal of Electrical Engineering and Technology
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    • 제7권1호
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    • pp.23-33
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    • 2012
  • The current paper deals with a new live-line insulator inspection tool system that will be used by the Korea Electric Power Corporation for its 154 kV power transmission lines in 2011. Unlike the existing contact type tools, the developed inspection tool automates parts of the insulator inspection process using a sensor and an actuator. The tool sustains its weight independently, not by a lineman. In addition, the inspection tool measures the insulation resistance of an insulator together with its distribution voltage, thus providing more information for analysis and diagnosis. Such characteristics improve the system's operation efficiency, measurement reliability, and usability. We confirmed its effectiveness through live-line field tests with actual power transmission lines.