• 미생물학회지
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• 제50권1호
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• pp.84-86
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• 2014

길항균을 생물 농약으로 개발하기 위해서는 저렴한 산업용 배지를 이용한 대량 생산 체계를 확립하는 것이 중요하다. 본 연구에서는 풋마름병 방제 효과가 뛰어난 Bacillus amyloliquefaciens SKU-78 균주의 배양조건을 확립하였다. 저가의 산업용 기질로는 콩가루와 옥수수 전분 배지가 균 생장에 가장 효과적이었고, 최초 pH 5.5, 배양 온도 $30^{\circ}C$, 교반속도 150-250 rpm의 조건으로 30 L fermenter를 이용한 배양에서 20 시간째에 최대 생균수($1.2{\times}10^{11}$ CFU/ml)에 도달하였다. 저가의 산업용 배지로 배양한 배양액을 관주 처리하였을 때 65%의 발병 억제 효과를 나타냄으로써 SKU-78 균주의 산업용 배지를 이용한 대량배양의 기초가 마련되었다.

• Journal of Ginseng Research
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• 제24권2호
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• pp.58-63
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• 2000

식물조직배양기술을 이용하여 인삼 root를 생산하고자, 식물생장조절물질로 유도된 인삼 root를 사용하여 최적액체배양조건을 RSM을 이용하여 조사하였다. 최적액체배양조건을 배지의 pH, sucrose 농도, nitrogen 농도, phosphate 농도의 3 level-4factor의 fractional factorial design에 의하여 조사한 결과, 인삼 root의 생장율은 최저 1.00g에서 최고 2.33g까지 나타났다. 다중회기분석으로 구한 model식을 가지고 등고분석과 3차원분석을 수행한 후 독립변수의 최저 또는 최고수준에서 조속변수가 최대치를 나타내지 않는 배지의 pH와 sucrose농도의 변수에 대하여 model식을 편미분한 결과 인삼 root의 최적액체배양조건은 pH5.6, sucrose 3.8%, nitrogen 50mg/L, phosphate 80.7mg/L로 예측되었다. 이렇게 결정된최적조건값들을 model식에 대입하여 얻은 예상치는 2.36g 이었다.

• KSBB Journal
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• 제11권5호
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• pp.565-571
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• 1996

Aspergillus oryzae A Tee 2114를 사용하여 $\beta$-mannanase의 생산에 영향을 주는 배지성분의 최적 화를 수행하였다. 탄소원인 locust bean gum의 농 도를 달리하여 발효를 수행하였다. Locust bean gum의 농도가 20 g/L 이상일 때는 초기의 점도가 높아 혼합이 거의 되지 않아 초기에 $\beta$-mannanase 의 생산이 지 연되는 현상이 나타났고. Locust bean g gum의 농도가 증가할수록 $\beta$-mannanase의 역가와 균체농도가 비례척으로 증가하였다. Locust bean gum 10 g/L 배지에서 여러 가지 질소원이 $\beta$-mann anase의 생산에 미치는 영향을 살펴 본 결과,B-man n nanase의 역가는 무기질소원 중에서는 $(NH-4)_2SO_4$가 좋았으며 유기질소원 중에서는 malt extract가 가장 좋았다. 여러 가지 무기엽의 최적화를 수행한 결과 KH,PO.가 $\beta$-mannanase의 생산에 중요한 인자임을 알 수 있었다. 배지최적화 결과 최적배지 로 locust bean gum 10 giLt malt extract 3 g/L, $(NH-4)_2SO_4 2 g/L, KH_2PO_4$ 10 g/L이 결정되었으며 이때 $\beta$mannanase의 역가는 거의 6 unit/mL에 접 근하였다. 최적배지를 사용하여 발효조에서 배양을 수행하였다. 발효조의 흔합효과로 배양초기에 나타 나는 $\beta$-mannanase 생산 지 연현상을 감소시 킬 수 있었고 배양시간도 단축할 수 있었다. 27시간 배양 한 후 $\beta$mannanase의 역가 9.7 unit/mL와 비 $\beta$-mannanase의 역가 1.9 unit/mg-cell-을- 얻었다. 이 때, 생산성은 0.36 unit/mL. h이었다.

• 한국버섯학회지
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• 제6권1호
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• pp.20-26
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• 2008

흰목이버섯 균주와 공생균을 수집하고 ITS 5.8S rDNA sequencing을 하여 유전자 서열을 분석하였다. Gene Bank Data homology search 결과 분리된 균의 rDNA 서열이 이 Tremella fuciformis AF042409의 rDNA 서열과 99% 일치하는 것으로 확인되었다. 그리고 함께 분리된 공생균은 같은 방법으로 Annulohhypoxylon stygium 으로 확인하였다. 분리된 T. fuciformis KG 103과 A. stygium KG 201 균주는 PD배지에서 각각 14 mm/14 days과 85 mm/14 days의 균사생육을 나타내었다. T. fuciformis KG 103 균주의 생육최적온도는 $25^{\circ}C$ (14mm/14days) 이었으며, $35^{\circ}C$ 고온과 $15^{\circ}C$이하 저온에서 균사 생장이 억제되었다. A. stygium KG 201은 흰목이버섯균과 유사한 최적온도를 나타내었다. T. fuciformis KG 103 균의 생육 최적 pH는 5.0이었으며, A. stygium KG 201도 pH 5.0에서 생육이 가장 왕성하였다. 흰목이버섯 종균용 최적 배지로 참나무톱밥 77.5%, 미강 20%, 석고 1.5%, 황백당 1%가 선정되었다. T. fuciformis KG 103과 A. stygium KG 201혼합 종균을 제조하고 흰목이버섯 자실체생산을 위한 병속재배 방법을 확립하였다. 콘코브(Corn cob) (77%와 52%)가 사용한 재료 중 최적의 자실체 성장률을 나타냈으며, 콘코브 함량을 줄일수록 생육이 저조하였다. 면실박과 참나무톱밥은 단독 사용시 생육이 저조하였고, 콘코브를 첨가시 수율이 증대되었다. 최적수분농도는 55%로 결정되었다.

• Journal of Microbiology and Biotechnology
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• 제22권10호
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• pp.1412-1422
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• 2012

The aim of this work was to establish the optimal conditions for production of carboxymethylcellulase (CMCase) by a newly isolated marine bacterium using response surface methodology (RSM). A microorganism producing CMCase, isolated from seawater, was identified as Cellulophaga lytica based 16S rDNA sequencing and the neighborjoining method. The optimal conditions of rice bran, ammonium chloride, and initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively, whereas those for production of CMCase were 79.9 g/l, 8.52 g/l, and 6.1. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for production of CMCase were 3.72, 0.54, 0.70, and 0.34 g/l. The optimal temperature for cell growth and the CMCase production by C. lytica LBH-14 were $35^{\circ}C$ and $25^{\circ}C$, respectively. The maximal production of CMCase under optimized condition for 3 days was 110.8 U/ml, which was 5.3 times higher than that before optimization. In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of CMCase by C. lytica LBH-14. The time for production of CMCase by a newly isolated marine bacterium with submerged fermentations reduced to 3 days, which resulted in enhanced productivity of CMCase and a decrease in its production cost.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.823-834
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• 2014

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of $50{\mu}M$ methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas $50{\mu}M$ salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of $500{\mu}M$ had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.

• KSBB Journal
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• 제25권1호
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• pp.79-84
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• 2010

In the present study, we isolated a new bacterial strain producing invertase (EC 3.2.1.26) and determined optimized culture condition in flask culture. The strain was identified as Bacilus flexus determined by the 16S rDNA sequencing method. The invertase was produced only in the sucrose medium as the sole carbon source. Potassium nitrate was an adequate nitrogen source for enzyme production, whereas meat peptone showed the highest bacterial growth. Enzyme production was increased about 2-fold when $MgSO_4\cdot7H_2O$ was supplemented to the growth media. The optimum temperature was found to be $30^{\circ}C$ for both enzyme production and bacterial growth. Invertase exhibited pH optima in the range 5.0-6.0 and have a temperature optimum at $40^{\circ}C$, similarly to other invertases found from different microbial sources. Several mineral ions (K and Fe) stimulated the invertase activity, whereas some bioelements (Ag, Mg, and Mn) inhibited enzyme activity. Under the optimized culture condition, the maximum enzyme production (over 250 units/mL) was achieved at 20 h. To the best of our knowledge, this is the first time to report on invertase production by Bacilus flexus.

• 미생물학회지
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• 제36권3호
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• pp.236-241
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• 2000

효모 Saccharomyces cerevisiae를 숙주 세포로 이용하여 활성형의 항균펩티드를 생산하기 위한 model system으로서 쉬파리 유래 defensin의 합성 유전자를 GAP promoter, mating factor $\alpha$1 (MF$\alpha$1)의 preprosequence 및 GAL7 transcription terminator의 조절하에 있는 재조합 plasmid pGMD18을 제작한 후 Saccharomyces cerevisiae에 형질전환하여 growth inhibtion zone assay를 통해 항균활성을 보유한 재조합 효모를 선별하였다. 재조합 효모의 성장 속도와 defensin의 분비능을 증가시키기 위하여 최적의 배지 조성과 배양조건을 결정하였다. 재조합 효모의 defensin 생산을 위한 최적 배양조건을 조사한 결과 0.4% yeast extract, 유기질소원 2% corn steep liquor, 탄소원 2.5% glucose, 0.05% $C_2CO_3$를 포함한 배지에서 초기 pH3 그리고 배양온도 $28^{\circ}C$의 경우가 세포성장과 defensin 의 항균활성이 가장 우수하였다. 이 조건으로 배양을 수행한 결과 YPD 배지에서 배양한 조건보다 약 2배의 세포 성장과 약 4배의 defenzin 생산을 확인하였다.

• 센서학회지
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• 제33권4호
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• pp.230-236
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• 2024

This study aimed to analyze the environmental factors affecting tomato growth by examining the correlation between weather and growth environment sensor data from P Smart Farm located in Gwangseok-myeon, Nonsan-si, Chungcheongnam-do. Key environmental variables such as the temperature, humidity, sunlight hours, solar radiation, and daily light integral (DLI) significantly affect tomato growth. The optimal temperature and DLI conditions play crucial roles in enhancing tomato growth and the photosynthetic efficiency. In this study, we developed a model to correct and predict the time-series variations in internal environmental sensor data using external weather sensor data. A linear regression analysis model was employed to estimate the external temperature variations and internal DLI values of P Smart Farm. Then, regression equations were derived based on these data. The analysis verified that the estimated variations in external temperature and internal DLI are explained effectively by the regression models. In this research, we analyzed and monitored smart-farm growth environment data based on weather sensor data. Thereby, we obtained an optimized model for the temperature and light conditions crucial for tomato growth. Additionally, the study emphasizes the importance of sensor-based data analysis in dynamically adjusting the tomato growth environment according to the variations in weather and growth conditions. The observations of this study indicate that analytical solutions using public weather data can provide data-driven operational experiences and productivity improvements for small- and medium-sized facility farms that cannot afford expensive sensors.

• 한국미생물·생명공학회지
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• 제23권1호
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• pp.6-11
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• 1995

In order to screen microorganism producing lactic acid with high productivity from nature, we used a medium containing 100 g/l glucose and selected several microorganisms producing more than 80 g/l L-lactic acid. We investigated their physiological characteristics and compared them. The best microorganism was identified as Lactobacillus casei subsp. rhamnosus. The optimum pH for growth and production of lactic acid was 6.0 and this strain showed the highest growth rate at around 30$\circ$C , but the optimum temperature for lactic acid production was 45$\circ$C . The growth was inhibited proportionally from 50 g/l to 300 g/l of glucose and the maximal cell mass increased according to increasing the concentration of corn steep liquor (CSL) protein up to 30 g/l. In batch fermentation for lactic acid production, we produced 128 g/l L-lactic acid with 20 g/l CSL protein and 150 g/l glucose in 35 hours. In pH-stat fed-batch fermentation, we were able to produce 183 g/l L-lactic acid.