• 생약학회지
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• 제18권1호
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• pp.34-40
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• 1987

Onion extracts were tested for effects on the growth and lactic acid production of Lactobacillus bulgaricus in the medium containing mercury. The media containing mercury were added with onion extracts and inoculated with the bacterium and then incubated at $39^{\circ}$ for four days. All of the onion extracts examined increased the growth and lactic acid production of the bacterium in the medium containing mercury. At the addition of the edible portion extract of onion to the medium containing 5 ppm, 10 ppm and 20 ppm mercury, the higher the concentration of the onion extract was added, the greater the increasing effect on the growth and lactic acid production. The brown peel extract of onion increased the growth and lactic acid production of the bacterium in the medium containing mercury at all concentrations. The higher the concentration of the extract added, the more effective the increasing effect. The onion solutions of edible portion and brown peel extracted at $90^{\circ}\;to\;100^{\circ}$ showed more desirable effects than those extracted at the room temperature. Among four kinds of the onion extracts, the brown peel solution extracted at $90^{\circ}\;to\;100^{\circ}$ was the most effective in increase of the growth and lactic acid production in the medium containing mercury.

• 대한약리학회지
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• 제31권1호
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• pp.85-93
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• 1995

Testosterone이 serum-free medium에서 배양한 토끼의 신장 근위세뇨관 상피세포의 세포성장과 기능에 미치는 영향을 관찰한 바 다음과 같은 결과를 얻었다. 1. 토끼의 신장 근위세뇨관 상피세포는 testosterone 1 nM의 농도에서 유의한 세포 성장 촉진 효과를 나타내었고, testosterone 10 nM이상의 농도에서는 세포성장이 억제되었다. 2. Testosterone은 serum-free medium에서 성장촉진인자의 하나인 hydrocortisone을 growth supplement로 넣어준 serum-free medium에서 토끼 신장의 근위세뇨관 상피세포의 성장을 촉진시키었다. 3. Testosterone은 hydrocortisone을 growth supplement로 넣어준 serum-free medium에서 토끼 신장의 근위세뇨관 상피세포의 성장을 촉진시키었다. 4. Testosterone은 Northern blot analysis에 의하여 확인한 토끼 신장의 근위 세뇨관 상피세포의 ${\beta}-actin$ mRNA level은 증가되었다. 이상의 결과로 미루어 보아, serum-free 그리고 hormonally defined media에서 testosterone이 토끼의 신장 근위세뇨관 상피세포의 성장 및 기능에 대하여 촉진적으로 작용하는 것은 cellular mecrofilament의 중요한 구성단백의 하나로 밝혀진 ${\beta}-actin$의 합성 증가에 기인하는 것으로 생각된다.

• 국토계획
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• 제54권3호
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• pp.63-74
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• 2019

Korean government has implemented policies to strengthen the competitiveness of small and medium sized cities. However, since it is often difficult to enhance the competitiveness through individual projects, many local governments in metropolitan areas are working together to pursue local growth. On the other hand, small and medium sized cities that are not included in metropolitan areas due to their spatial limitations have difficulties in implementing effective growth policies. Given this background, the purpose of this study is to identify the functional correlation based on urban interactions and develop functional econometric model for the economic growth of small and medium sized cities. This study uses spatial econometrics models and functional weight matrix to identify the effects of functional networks on small and medium sized cities. The results show the effect of functional networks on the growth of small and medium sized cities and provide policy implications for regional spatial planning that addresses effective management of small and medium sized cities.

• Journal of Plant Biotechnology
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• 제6권4호
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• pp.241-246
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• 2004

The bulb scales and shoot sections ($7\;\cal{mm}\;\times\;15\;\cal{mm}$) of Lilium oriental hybrid 'Casablanca' were cultured to compare bulblet growth in vitro. Shoots were induced from in vitro grown bulbscales on MS medium with $1.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA, and 30 g/L sucrose. The regenerated shoots were cut into shoot sections, and cultured on MS medium with $2.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA and 30 g/L sucrose for shoot proliferation. Culture of shoot sections stimulated bulblet growth significantly than the bulb scales on MS medium with 60 g/L sucrose. However, the bulblets from shoot sections did not reach ideal size to produce stems with several leaves. Therefore, liquid medium was added into the same vessels to stimulate bulblet growth further. After shoot sections were cultured on MS medium with 60 g/L sucrose and 2 g/L activated charcoal for two months in dark, $20\;\cal{ml}$ liquid media containing various concentrations of sucrose and MS salts were added. Two months later, the added liquid medium stimulated bulblet growth remarkably as compared to bulblets grown without added liquid medium. The added $25\;\cal{ml}$ liquid medium containing 120 g/L sucrose and double strength of MS salts were the most effective for growth of in vitro bulblets. More than $94\%$ bulblets produced by this method sprouted stems with several leaves after cold treatment at $5^{\circ}C$ for three months.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.132-137
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• 2016

The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing $10g{\cdot}L^{-1}$ sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with $10g{\cdot}L^{-1}$ sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with $10g{\cdot}L^{-1}$ sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

• 대한약리학회지
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• 제29권1호
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• pp.73-83
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• 1993

Steroid hormone의 하나인 ${\beta}-estradiol$이 serum-free medium에서 배양한 토끼의 신장 근위세뇨관 상피세포의 세포성장과 기능에 미치는 영향을 관찰한 바 다음과 같은 결과를 얻었다 1. Serum-free medium에서 토끼의 신장 근위세뇨관 상피세포는 ${\beta}-estradiol$ 1 nM의 농도에서 유의한 세포 성장 촉진 효과를 나타내었고, ${\beta}-estradiol$ 10 nM이상의 농도에서는 세포성장이 억제되었다. 2. ${\beta}-Estradiol$은 serum-free medium에서 성장촉진인자의 하나인 hydrocortisone을 뺀 조건하에서 세포 성장을 증가시키었다. 3. ${\beta}-Estradiol$은 hydrocortisone을 growth supplement로 넣어준 serum-free medium에서 토끼 신장의 근위세뇨관 상피세포의 성장을 촉진시키었다. 4. ${\beta}-Estradiol$은 Northern blot analysis에 의하여 확인한 alpha I (IV) collagen mRNA level에는 별다른 변화를 보이지 않으나, ${\beta}-actin$mRNA level은 증가되었다. 이상의 결과로 미루어 보아, serum-free 그리고 hormonally defined media에서 ${\beta}-estradiol이$ 토끼의 신장 근위세뇨관 상피세포의 성장 및 기능에 대하여 촉진적으로 작용하는 것은 cellular microfilament의 중요한 구성단백의 하나로 밝혀진 ${\beta}-actin$의 합성 증가에 기인하는 것으로 생각된다.

• 미생물과산업
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• 제19권3호
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• pp.41-45
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• 1993

The cultural conditions of the osmophilic red color yeasts (Strain L$_{1}$, L$_{2}$, L$_{3}$ and L$_{4}$) isolated and identified in the previous report were examined and the results obtained were as follows ; 1. The optimum medium for growth of these osmophilic red color yeasts was soy sauce medium. 2. These strains were grown exceedingly well on the medium containing 3 percent of NaCl but somewhat restrained on the medium containing 6% or more. 3. The optimum temperautre for growth of these strains was 25.deg.C and their lethal temperature was 68.deg.C(treatment for 5 minutes). 4. The optimum pH for growth of these strains was 6.0.

• 농업과학연구
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• 제47권4호
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• pp.1039-1048
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• 2020

Food waste causes various economic losses and environmental pollution problems such as soil pollution and groundwater pollution. Food waste has been used as a resource in various forms and has been used mostly for feed and composting. This study compared microalgal nutrient medium (BG-11) with food waste compost to determine the possibility of using it as a culture medium. Scenedesmus quadricauda was isolated and cultured in an eutrophic reservoir and incubated for 3 days in distilled water before laboratory use. Food waste compost was produced in two food waste processing facilities, and hot water was extracted in the laboratory to be used for microalgae cultivation. The growth curve of the microalgae was analyzed based on the Chl-a concentration measured during the experiment, and the growth rate of the microalgae grown in the food waste compost was compared with the growth rate of those grown in the nutrient medium. Food waste compost showed a similar growth rate to that of the nutrient medium, and there was a difference depending on the manufacturing facility. The growth of microalgae in such food waste was further amplified when trace elements were added and showed better growth than that of the nutrient media. Particularly, when trace elements were added, the growth rate increased, and the growth period was further extended. Therefore, food waste compost can be sufficiently utilized as a microalgal culture medium, and if trace elements are added, it is considered that microalgae can be more effectively cultured compared to the existing nutrient medium.

• Korean Chemical Engineering Research
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• 제54권6호
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• pp.812-816
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• 2016

We isolated a novel methylotrophic bacterium from soil collected in Dongducheon Environment Affairs Agency. The isolate was identified as Methylobacterium sp. WJ4 based on phylogenetic analysis. Plackett-Burman design was employed for screening eight parameters of nitrate mineral salts (NMS) medium for cell growth of a newly isolated Methylobacterium sp. WJ4 with experimental validation. Trace element solution and vitamin stock were found to affect cell growth, which can be further optimized for increased cell growth. This is the first report of screening parameters of NMS medium which affect cell growth of strain belonging to the genus Methylobacterium using Plackett-Burman design.

• 한국산학기술학회논문지
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• 제21권10호
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• pp.64-71
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• 2020

본 연구에서는 미세조류 Haematococcus pluvialis의 성장에 미치는 배양 배지와 광원의 영향을 평가하기 위해 제한 성분 배지인 Modified Bold's Basal Medium (MBBM) 와 상업용 액체 비료 배지인 Neo, 그리고 파장이 서로 다른 7개의 광원을 사용하여 H. pluvialis를 39일 동안 배양하고 성장률을 비교하였다. 그 결과 제한 성분 배지인 MBBM에서 H. pluvialis의 성장은 fluorescent light 광원에서 세포 성장이 가장 높게 나타난 반면, blue+red LED에서 세포 성장이 가장 낮게 나타났다. 상업용 배지인 Neo에서 H. pluvialis의 성장은 fluorescent light 광원에서 세포성장이 가장 높게 나타났으며, blue LED에서는 세포 성장이 가장 낮음이 확인되었다. 본 연구를 통해 MBBM 배지가 Neo 배지보다 우수한 결과를 나타내었으며 또한, MBBM 배지를 이용하여 fluorescent light에서 성장한 미세조류는 본 연구에서 가장 우수한 세포 성장 결과를 보였다. 이러한 결과는 이차대사산물 생산을 위한 H. pluvialis 배양에서 배지, 광원과 광량을 최적화한 것으로 미세조류 대량 배양에 기초 자료로써 유용하게 활용될 것이다.