• Food Science and Preservation
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• v.6 no.4
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• pp.506-513
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• 1999

The growth of lactic acid bacteria was investigated in liquid broth medium containing organic germanium compound(Ge-132, carboxyethylgermanium sesquioxide) in the range of 0.01 to 10mg/ml. Most of all lactic acid bacteria tested were tolerant and could grow better to the high Ge-132 concentration. However, the growth of Leuconostoc mesenteroides and Pediococcus pentosaceus were inhibited in the presence of 10mg/m1 Ge-132. Among 22 strains tested, lactic acid bacteria that were grown to a high degree(about 2 times) by addition of Ge-132 (10mg/ml)were Lactococcus lactis, Lc. cremoris, Lc. diacetilactis, Enterococcus faecium and Streptococcus faecalis. The growth of these strains were markedly accelerated in the culture medium supplemented with 1.omg/ml Ge-132 The optimal concentration of glucose for growth of Lc. lactic was found to be high in medium containing Ge-132 as compared with the case of control. During cultivation viscosity in culture broths of Lc. lactis and Lc. cremoris was rapidly elevated by adding Ge-132 to medium containing high concentration of glucose, and then decreased after incubation of long time. However, in the cultivation of Lc. diacetilactis, E, faecium and S. faecalis, viscosity of culture broths was not increased, even though Ge-132 was shown to be an effective stimulant of growth.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.49-54
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• 2018

To select the superior strain of Haesongi, Hypsizigus marmoreus, strains were isolated by Di-Mono mating with isolates from different fruit bodies. Three strains were selected to superior isolates that are good mycelial growth on PDA medium for 10days. When cultured on PDA medium for 10 days, strain No. 3 and strain No. 7 showed mycelial growth of 62mm and 58mm, respectively. Mycelial growth was good in the order of strain No. 2, 10, and 9. The three selected strains, KNCAF-H-3, KNCAF-H-7 and KNCAF-H-2, were cultured in sawdust medium for 10 days and showed mycelial growth of 79mm, 76mm and 73mm respectively. The mycelial growth of the selected three cultivars was better than that of Greenpeace No 5, a control cultivar grown at 55mm.

• Resources Recycling
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• v.23 no.1
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• pp.64-69
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• 2014

For the recovery of Co and Cu, bioleaching behavior of Co, Cu, Fe, Mg, Al by Aspergillus niger culture from the molasses growth medium was investigated. Series of leaching tests have been conducted by varying Aspergillus niger's type, molasses concentration in the growth medium, pulp density and reaction time. The results showed that increase of the molasses concentration in the growth medium from 1% to 4% increased the leaching percentage of Co and Cu and the optimal molesses concentration was found to be 4% in the growth medium. Maxinum 90% of Co and 70% of Cu were dissolved from the leaching test at the 10 g/L pulp density, 4 % of molasses concentration in the growth medium after 21 days by Aspergillus niger KCTC 6985. But in case of using Aspergillus niger KCTC 6144, the maxium leaching percentage of Co and Cu was reached 90% respectively at a pulp density 5 g/L and 4% of molasses concentration.

• KSBB Journal
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• v.7 no.2
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• pp.92-95
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• 1992

A model for the role of serum was proposed to develop a low serum medium for the large scale cu1ture of mammalian cell. The strategy of medium development adopted in this study facilitated the understanding of the role being carried out by the serum in the culture of hybridoma KAl12 cell line. In this model, the serum components were divided into two main groups : the first group encompasses the nutrient factors that determine the maximum cell density and the second group includes the growth factors that regulate the cell growth rate, The model prediction was compared with the experimental results. The model enabled us to find out several useful aspects of medium composition for cell growth. 1) One particular component in the basal medium became limiting factor when serum concentration level was more than 7%. 2) The growth regulating factors and nutrient factors limited the cell growth at 3% and 5% serum concentration levels respectively.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.207-211
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• 2010

Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.

• Journal of Korean society of Dental Hygiene
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• v.8 no.3
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• pp.139-148
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• 2008

A fluorine solution was used to measure the growth of S.mutans and the pH changes were also measured in order to find an effective and preventative dentistry lab within a limited time for the dental hygiene department curriculum and the following results were obtained. 1. In the growth comparison of S.mutans, the culture medium of the experiment group with fluorine weighed 0.3g and the culture medium with no fluorine weighed 0.5g, which shows that the growth rate of S.mutans is significantly decreased in the culture medium with the fluorine. 2. The pH7.8 of the culture medium was not nearly changed; it became 7.0 after culturing with fluoride, however the pH was significantly decreased to 3 in the culture medium that had no fluorine. 3. Since it has been proven that the fluorine can control the growth of germs, it is believed that the effect of fluorine as a cavity preventative should be emphasized to students and in addition, it will help students transmit the effect of fluoride to their patients since this knowledge has been acquired through practice. 4. It is considered that this is a simple test protocol providing effective results in the microorganism and preventive dentistry lab within a limited time and furthermore, it will furnish the students of dental hygiene with comprehensive accessibility to dental hygiene curriculum.

• Journal of Life Science
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• v.14 no.4
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• pp.560-566
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• 2004

Agrocybe aegerita is Hymenomycetes fungus belonging to the order Agaricales and family of Bolbitiaceae. Much less is known about liquid culture of Agrocybe aegerita. Thus, the present study was to investigate the liquid cultural characteristics of Agrocybe aegerita my-celium. The optimal medium for the mycelial growth and density was ME medium, optimal tem-perature and initial pH were 25$\pm$1$^{\circ}C$ and 5.5, respectively. And optimal culture time for mycelial growth was 12 days. The modified optimal medium compositions were dextrin 3% (w/v), yeast extract 2% (w/w), MgSO$_4$0.05% (w/v), and KH$_2$PO$_4$0.15% (w/v). Under optimal culture conditions, the mycelial growth of modified optimal medium was higher than that of ME medium.

• Applied Biological Chemistry
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• v.39 no.5
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• pp.343-348
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• 1996

Experiments were carried out to find possibility of economic production of SCP in mixed culture by Cellulomonas sp. KL-6 and E. coli LI-10. The best cell growth was obtained at the ratio of 1 : 1(v/v) in mixed culture. When these strains were mixed culture, cell growth was increased to about 63%, compared with those of single culture of strain KL-6. It was found that the majority of the population during growth in mixed culture consisted of strain KL-6. $CaCO_3$ added to the medium as the ratio of 0.1% was enhanced medium pH. Cell growth increased in that circumstances. These strains produced much amounts of cellobiose, but glucose was not detected in filter paper medium. When these organisms were cultured under the optimal medium for 4 days, cell mass was produced $1.0\;g/{\ell}$. The results showed the increase of cell mass up to 53% than those produced in CMC medium.

• Journal of People, Plants, and Environment
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• v.23 no.5
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• pp.545-554
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• 2020

Background and objective: Moth orchids in the vegetative stage are suitable for a multi-layer growing environment in a closed-type plant factory which can be a good alternative that can reduce production costs by reducing cultivation time and energy cost per plant. This study was conducted to find out the optimal rhizospheric environment for different irrigation methods without a potting medium and rhizospheric ventilation for the vegetative growth of young Phalaenopsis hybrid 'Blanc Rouge' (P. KV600 × P. Kang 1) and Phalaenopsis Queen Beer 'Mantefon' in a closed-type plant factory system. Methods: The one-month-old clonal micropropagules with bare roots rapped with a sponges were fixed on the holes of styrofoam plates above growth beds, and were watered using the ebb-and-flow (EBB) and aeroponic (AER) methods with Ichihashi solution (0.5 strength) once a day at 06:00 (P) or 18:00 (S), and both (PS). Rhizospheric ventilation (V) was also applied to change the temperature, relative humidity, and CO₂ concentration of the beds. Plants potted into sphagnum moss and watered once a week were used as the control group. Results: After 12 months of treatment, the growth characteristics of the EBB groups were the best among the treatment groups without a medium, but no effect of irrigation timing was observed. V reduced the temperature, relative humidity and CO₂ concentration of the beds. Whereas, EBB+V (ebb-and-flow with ventilation) improved plant growth and reduced the occurrence of disorders and withering. Especially, EBB+V showed a similar performance to the control group. Conclusion: The results indicated that the optimal irrigation method without a potting medium for producing middle-aged potted moth orchids was the EBB system with forced rhizospheric ventilation. Therefore, further studies on the optimal ventilation method and moisture control of the crown need to be carried out to develop the irrigation system without a potting medium for vertical farming in closed-type plant factories.

• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.141-144
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• 2001

To enhance the shoot induction efficiency from nodal stem of yooza, the culture conditions such as basal medium, carbohydrate source, solidifying agent and the optimum concentration of plant growth regulators for shoot induction were investigated. The nodal explants were cultured better on MS medium than on MT, SH, B5 or W media in considering of shoot induction rate and mean shoot length. Solidifying agent in medium was better with 0.8% agar than with 0.3% agar, 1.2% agarose or 0.2% gelrite. Carbohydrate source in shoot induction medium was efficient with 30 g/L sucrose. The optimum concentrations of plant growth regulators were determined that 0.1 mg/L NAA as auxin was effective on the shoot induction, and 1.0 mg/L BAP as cytokinin induced multiple shoots efficiently. Shoot induction was the most effective on MS medium supplemented with 4 mg/L $GA_3$ in yooza.