• Title/Summary/Keyword: growth medium

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Optimal Condition of Mycelial Growth of Grifola frondosa (잎새버섯 균주의 균사체 생육 최적조건)

  • Chi, Jeong-Hyun;Kim, Jeong-Han;Won, Sun-Yee;Seo, Geon-Sik;Ju, Young-Cheoul
    • The Korean Journal of Mycology
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    • v.35 no.2
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    • pp.76-80
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    • 2007
  • This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and investigated its optimal culture condition. Among four kinds of mushroom culture media, PDA medium was selected as the suitable culture medium. The optimal conditions for the mycelial growth of G. frondosa in PDA medium were $25^{\circ}C$ and $4{\sim}5$ of pH, respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively, and the highest mycelial growth was observed when the C/N ratio was $10{\sim}20$.

Change in Growth of alcohol Fermentation Yeast with Addition of Deep Seawater (해양 심층수 첨가에 따른 알콜발효 효모의 증식 변화)

  • 김미림;정지숙;이기동
    • Food Science and Preservation
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    • v.10 no.3
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    • pp.417-420
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    • 2003
  • In order to study optimum culture condition of yeast medium added deep seawater, we examed samples with 9 yeast strains. The growth rate were measured for Saccharomyces cerevisiae 10, 11, 12, 901 and RCY and Saccharomyces kluyvery DJ97, Saccharomyces cerevisiae YJK, JK99, CMY-28 etc.. The growth of S. cerevisiae 12 was found most active in the deep seawater(hardness 500). The growth rate of S. cerevisiae 901 on medium containing deep seawater(hardness 1000) was faster than that of the yeast on medium without deep seawater. The use of deep seawater on the growth of Sacch.cerevisiae kluyvery DJ97 revealed maximum growth under the condition of hardness 200 of deep seawater and 10% of sugar concentration.

The Contribution of Small and Medium-Sized Enterprises in the Economic Growth of the Southeast Region of Vietnam

  • PHAM, Thanh Van;NGUYEN, Van Luan;NGUYEN, Thi Lai;PHAN, Thi Thu
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.9
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    • pp.155-163
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    • 2021
  • This research was conducted to check the impact of factors related to the small and medium-sized enterprises (SME) on the economic growth in the Southeast region of Vietnam, over the years from 1996-2019. This paper applies a combination of FEM, DKSE, GMM, and RIDGE-FEM regression methods to estimate the influence of independent variables on the economic growth of the whole Southeast region with the panel data collected from GSO; and applying the OLS regression model for each province. The study finds that all variables have a statistically significant positive impact on the economic growth of the study area. Accordingly, the importance of the variables is in the following order: (1) the proportion of workers by professional and technical qualification (SMEH), (2) the number of vocational training schools (LnTSCH), and educational level of workers (LnSchool), (3) the number of SME enterprises (LnSME); (4) The average number of years in the schooling of employees in the enterprise (LnSchool); (5) Enterprise capital (LnCAP); and (6) the average number of employees of SME (LnSMER). The research results also show that factors related to the quality of labor resources have a more positive influence on growth than both the labor size and financial capital of SMEs.

The Impact of Financial Management Practices on Growth of Small and Medium Enterprise in MOGADISHU

  • Mohamud, Isse Abdikadir;Mohamed, Abdisalan
    • East Asian Journal of Business Economics (EAJBE)
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    • v.4 no.3
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    • pp.25-30
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    • 2016
  • Small and Medium enterprises (SMEs) make important contributions to economic and social development of the country. The aim of this study was to determine the extent of financial management practices employed by the SME's and their effect on growth. This study was conducted quantitatively through descriptive research design; the data was collected from sample size of 120 of some SMEs operate in Mogadishu. Used questionnaire as data collection tool, the data analysis was done by using the statistical package SPSS version16, the data analyzed and presented by calculation, determining frequency, percentages, mean and standard deviation. Therefore the researchers found result revealed that extent of financial management is low among small and medium enterprises and also, that the financial management is significantly influence on the on growth of small and medium enterprises in Mogadishu Somalia. Furthermore the findings revealed that the cash management, accounts receivable and inventory management are low among SME. Also that most of the selected SME don not make financial plan to their business, at the end that most of the selected SME don not have formal accounting information system to their business.

Develpment of Low-serum Medium(LSM) for Mouse-mouse Hybridoma Part II. Development of Low Serum Medium by Screening for Serum Replacement (Hybridoma 배양을 위한 저혈청 배지의 개발 제2부: 혈청 대체 물질 선정을 통한 저혈청 배지 제조)

  • 제훈성;최차용
    • KSBB Journal
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    • v.7 no.2
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    • pp.96-101
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    • 1992
  • A low serum medium(LSM) suitable for the growth of a self-constructed hybridoma cell line, KA112, was established by selecting ingredients to replace serum. Insulin and sodium pyruvate was important for the growth of cell line KA112. Various basal media were tested and DMEM gave the most favorable result. Low serum medium (LSM) developed in this work showed cell line stability in the culture for more than 6 months and exhibited cell growth equivalent to that carried out in medium supplemented with 7% FBS. LSM was found to be applicable to the suspension culture of KA112. The reduction of serum level down to 1%(V/V) FBS in LSM resulted in a substantial saving in the cost of media preparation for large scale culture.

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Stimulating effects of Chlorella and Scenedesmus cell upon the growth and fermentation of L. delbrukii and B. subtilis (Chlorella와 Scenedesmus 세포 함유물질이 Lactobacillus delbrukii 와 Bacillus subtilis 에 미치는 성장 촉진효과)

  • 정지원;이태우;이주식
    • Korean Journal of Microbiology
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    • v.6 no.1
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    • pp.12-21
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    • 1968
  • The accelerate effects on the growth rate and the capacity of fermentation of L. delbrukii and B. subtilis was investigated in the Henneberg's medium added by various amounts of cellular components of Chlorella and Scenedesmus and also was investigated in the media added micronutritional elements such as Mn, Fe and Mo, etc. The results in the comparative experiments are as follow; 1. Various amounts of Chlorella cell components in the media accelerated remarkably the lactic acid formation and growth of L. delbrukii. For example, lactic acid formation in the medium of contained 1 percent Chlorella cell components was promoted more than twice effects compare with control. 2. The formation of .alpha.-amylase by B. subtilis in the medium of 2 percent Chlorella cell contents was also promoted more than nine twice effects compare with control. 3. The formation of lactic acid of L. delbruckii in the medium of Scenedesmus cell contents was a little more than in the medium of Chlorella cell contents. 4. The lactic acid fermented level attained with the addition of 0.2-0.25 percent Chlorella cells was the effect of promoting fermentation attained of saturating level at 100$\mu$g. /ml. of Mn and 0. 1 $\mu$g./ml. of Fe.

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A METHOD OF MUCOSA CULTURE (구강점막의 배양에 관한 연구)

  • Choi, Byung-Ho;Yoo, Jae-Ha
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.17 no.4
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    • pp.331-336
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    • 1995
  • To use cultured mucosa as a graft of full thickness, our laboratory has been involved in the development of techniques to grow epidermis together with connective tissue. Human oral mucosa was obtained at dental surgery. Under sterile conditions the tissues were cut into explants of 0.1 $cm^2$ which were placed in the center of 24 well tissue culture dishes and incubated in a growth medium. The growth medium used for epithelial was MEM(Minimum Essential Medium) supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide, glutamine (0.292 g/l), epidermal growth factor (40 ug/ml), cholera toxin (30 ng/ml), hydrocortisone (2 ug/ml), insulin (40 ug/ml) and transferin (5 ug/ml). The medium for stratification of epithelial cells was MEM supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide and glutamine (0.292 g/l). The medium used for fibroblasts was MEM supplemented with 10% fetal calf serum. With the three types of media used alternatively, a mucosa composed of epidermis and connective tissue was obtained after 3 weeks of culture.

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Effect of Inorganic sale, Growth Regulators, and Thiamine on the Callus Growth and Plant Regeneration from long-term Cultured Solanum and Lycopenicon Genotypes (무기염류, 생장조절물질 및 타이아민이 장기간 계대배양한 Solanum과 Lycopnicon종의 캘러스 생장과 식물체분화에 미치는 영향)

  • Chang Yeon YU;Byong Ho CHANG;Dong Ha CHO
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.2
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    • pp.69-76
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    • 1994
  • Callus growth and shoot regeneration of Solanum and Lycopersicon depended on genotype, growth regulators, and thiamine concentration. L. peruvianum LA 1277 and L. peruvianum LA 1373 and PI 251301 had the greatest callus growth while L hirsutum LA 1777, L.esculentum 'Diego'and 'Red Plum' had the least callus growth. Lycopersicon penvianum genotypes were superior to L. esculentur genotypes in regenerating plants. MG medium was more effective in regenerating shoots than MS medium. A low level of IAA (0.2mg/L) and high level of BA (2 mg/L) resulted in the greatest shoot regeneration. Shoot growth varied depending on thiamine concentration and genotype. Shoot proliferation of Solanum ptycathum, Solanum nigrum, and L. peruvianum PI 199380 was best on medium with 20 mg/L thiamine. Regeneration of L. peruvianum PI 251301 and PI 128652 was better on medium with 30 and 10mg/L thiamine, respectively.

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Effects of pH, Sucrose and Vitamins on the Growth and Tropane Alkaloid Production of Hairy Roots of Datura stramonium var. tatula Torr. (독말풀(Datura stramonium var. tatula Torr.) 모상근의 성장과 tropane alkaloid 생성에 미치는 pH, 서당 및 비타민의 영향)

  • 양덕조;강현미;이강섭;김용해;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.143-148
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    • 1997
  • The optimum concentrations of pH, sucrose and vitamins for the growth and tropane alkaloid production of hairy root clone DTLA9 (best growth line) were investigated. The optimum pH in growth and tropane alkaliod production of DTLA9 clone in SH (Schenk and Hildebrandt, 1972) basal medium without growth regulator were pH 6.3 and 6.5, respectively. Also, the optimum sucrose concentration in growth and tropane alkaliod production in the same medium were 3.0 and 2.8%, respectively. The optimum concentrations of ascorbic acid, D-pantothenate, nicotinic acid, pyridoxine, riboflavin, and thiamine on the growth of DTLA9 clone in SH basal medium without vitamins were 0.1 mM, 0.003 mM, 0.07 mM, 0.002 mM, 0.025 mM, and 0.01 mM, respectively. In particular, supplement of 0.1 mM ascorbic acid to SH basal medium without vitamins stimulated the tropane alkaloid production.

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Effect of Germanium-132 on the Growth of Lactic Acid Bacteria (젖산균의 성장에 미치는 Ge-132의 영향)

  • Park, Seok-Kyu;Lee, Sang-Won;Takafumi Kasumi
    • Food Science and Preservation
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    • v.6 no.4
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    • pp.506-513
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    • 1999
  • The growth of lactic acid bacteria was investigated in liquid broth medium containing organic germanium compound(Ge-132, carboxyethylgermanium sesquioxide) in the range of 0.01 to 10mg/ml. Most of all lactic acid bacteria tested were tolerant and could grow better to the high Ge-132 concentration. However, the growth of Leuconostoc mesenteroides and Pediococcus pentosaceus were inhibited in the presence of 10mg/m1 Ge-132. Among 22 strains tested, lactic acid bacteria that were grown to a high degree(about 2 times) by addition of Ge-132 (10mg/ml)were Lactococcus lactis, Lc. cremoris, Lc. diacetilactis, Enterococcus faecium and Streptococcus faecalis. The growth of these strains were markedly accelerated in the culture medium supplemented with 1.omg/ml Ge-132 The optimal concentration of glucose for growth of Lc. lactic was found to be high in medium containing Ge-132 as compared with the case of control. During cultivation viscosity in culture broths of Lc. lactis and Lc. cremoris was rapidly elevated by adding Ge-132 to medium containing high concentration of glucose, and then decreased after incubation of long time. However, in the cultivation of Lc. diacetilactis, E, faecium and S. faecalis, viscosity of culture broths was not increased, even though Ge-132 was shown to be an effective stimulant of growth.

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