• 원예과학기술지
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• 제16권2호
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• pp.233-235
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• 1998

Energized 기능수는 미약 에너지 발생 신소재로서 다음과 같은 공정을 거쳐서 경원생명과학연구소에서 제조되었다; 지하수 순수화 처리촉매제 첨가 에너지 imprinting in platinum column 재료혼합 energized water + 특수처리된 zeolite + 광합성 세균균주 발효 (25C) 여과 최종산물 energized 기능수 (여과액) + energized powder (잔유물), 후지사과 나무가 발아하기 전에 주당 3kg의 energized power를 수관하부에 살포하여 토양과 혼합하고 energized 기능수를 생육기 동안 3회에 걸쳐 토양관주한 결과 수확된 과실의 과피와 과육에 Ca 함량이 증가된 반면 잎과 신초 수피조직중의 Ca 및 N함량은 감소되었다. Energized 기능수 처리는 사과나무 잎의 순광합성율과 뿌리활성을 촉진시켰다. 또한 과실특성에 있어서 가용성 고형물과 안토시아닌 함량이 현저히 증가되었고, 과실의 저온저장중 과실경도가 높게 유지되었으며 과심부 갈변현상이 감소되었다. 과실의 유기산 함량은 처리간에 차이가 없었다.

• 한국컴퓨터정보학회논문지
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• 제6권4호
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• pp.94-99
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• 2001

패킷 전송망에서의 멀티미디어 데이터 전송은 송수신 단말간에 다양하게 나타날 수 있는 가변적 지연으로 인해 수신지에 도착하는 데이터 패킷간 지연 시간의 차이인 지터와 손실이 발생하므로 수신지 데이터들의 동기화를 이루지 못하게 된다. 특히, 근래의 인터넷과 같은 망의 사용이 급격히 증가함에 따라 나타나는 스파이크(spike)성 지연〔8〕에 대해서는 적절한 동기화 조치가 취해지지 못하고 있다. 본 논문에서는 스파이크성 지연으로 인한 손실을 줄이고 수신지 데이터들의 동기화를 위해 동기 구간을 조정하여 통신망의 급격한 변화에 잘 적응할 수 있도록 하는 적응형 동기화 알고리즘을 제안한다. 또한 임의의 패킷망에서 전형적으로 나타나는 지연 분포 특성을 이용하여 재생 시간을 결정하는 방법을 제안하고, 패킷 손실률과 지연 시간에 대해 성능을 평가한다.

• The Korean Journal of Physiology and Pharmacology
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• 제25권6호
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• pp.497-506
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• 2021

Besides using for hair removal, depilatory agents have been considered to be used as a penetration enhancer for transepidermal drug delivery. To examine the effect in hair follicles (HFs), two commercially available depilatory creams were tested on the dorsal skin of mice to monitor the effect deep into the skin structure. Fifteen male BALB/c mice were used in this study. Depilatory creams were applied to the dorsal skin of the same animal using shaved and untouched treatments as controls to minimize individual differences. Skin samples were collected at three days, one week and two weeks (n = 5 for each) after the treatment, and subjected for hematoxylin-eosin staining, and immunohistochemical analysis for proinflammatory cytokines. The morphological examination showed an increase in the thickness of epidermal layer of the depilatory cream-treated skin at early time points and in the subcutis at two weeks. Depilatory cream promoted entry of anagen phase and increased the number of hair follicles in the subcutis at one and two weeks. Immunohistochemistry showed elevated percentages of dermal fibroblasts expressing interleukin-6, tumor necrosis factor-α, and tumor necrosis factor-β. Shaving process increased the thickness of epidermis and dermis as depilatory creams did, but did neither induce the expression of proinflammatory cytokines in the dermal fibroblasts nor the number of HFs. The results suggested that the commercially available depilatory creams caused a transient minor inflammatory response of the skin and increased the levels of cytokines that might subsequently affect hair growth.

• Journal of Korean Academy of Oral Health
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• 제42권4호
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• pp.224-228
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• 2018

Objectives: As a first step to study the anticaries effect of ethanol alone, we investigated the effects of ethanol on the expression levels of the atpB gene and proton permeability of Streptococcus mutans in suspension cultures. Methods: S. mutans UA159 was grown in brain heart infusion medium at either pH 4.8 or 6.8. The total extracted RNA was reverse-transcribed into cDNA using a $Superscript^{TM}$ First-Strand Synthesis System. The resulting cDNA and negative controls were amplified by ABI PRISM 7700 real-time PCR system with SYBR Green PCR Master Mix. For proton flux assay, bacterial suspensions were titrated to pH 4.6 with 0.5 M HCl, and then additional 0.5 M HCl was added to decrease the pH values by approximately 0.4 units. The subsequent increase in pH was monitored using a glass electrode. Ten percent (v/v) butanol was added to the suspensions at 80 min to disrupt the cell membrane. Results: In a concentration-dependent manner, ethanol alone not only decreased the growth rate of S. mutans and the expression of the atpB gene but also increased the proton permeability at both pH 4.8 and 6.8. Conclusions: These findings suggest that ethanol has the potential for an anticaries ingredient. We believe that ethanol may be used together with fluoride and/or other cariostatic agents in order to develop better anticaries toothpastes and/or mouthrinses.

• 동의생리병리학회지
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• 제33권4호
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• pp.191-197
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• 2019

This study aims to evaluate the effects of the root bark of Morus alba (RMA) on the regulation of the Hippo-YAP pathway. Hippo-YAP signaling is a critical regulator in controlling organ size and tissue homeostasis. Hippo, the serine/threonine kinase phosphorylate the LATS. Phosphorylated LATS then phosphorylates and inactivates the YAP and TAZ, which are two closely related transcriptional co-activator. Here we report RMA activates the Hippo signaling, thereby inhibits the YAP/TAZ activity. First, we examine the cytotoxic effects of RMA by MTT assay. RMA was cytotoxic at concentrations higher than $50{\mu}g/ml$ in HEK293A cells. The reporter gene assay was performed to measure the activity of TEAD, a key transcription factor that controls cell growth and proliferation. RMA significantly suppressed the luciferase activity. By phos-taq gel shift assay, and western blotting, we showed that RMA enhanced the phosphorylation of YAP in wild type cells, but not in LATS1/2 knock out cells, which means RMA activates classical Hippo pathway. RMA induced the cytoplasmic sequestration of YAP. RMA also suppressed the mRNA expression of CTGF and CYR61; the two major YAP dependent genes. Taken together, RMA is considered to be a good candidate for proliferative disease such as cancer, by facilitating cell death through activating the Hippo signaling pathway.

• Clinical and Experimental Pediatrics
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• 제64권7호
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• pp.355-363
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• 2021

Background: Nephrotic syndrome (NS) is a common renal disorder in children attributed to podocyte injury. However, children with the same diagnosis have markedly variable treatment responses, clinical courses, and outcomes, suggesting molecular heterogeneity. Purpose: This study aimed to explore the molecular responses of podocytes to nephrotic plasma to identify specific genes and signaling pathways differentiating various clinical NS groups as well as biological processes that drive injury in normal podocytes. Methods: Transcriptome profiles from immortalized human podocyte cell line exposed to the plasma of 8 subjects (steroid-sensitive nephrotic syndrome [SSNS], n=4; steroid-resistant nephrotic syndrome [SRNS], n=2; and healthy adult individuals [control], n=2) were generated using microarray analysis. Results: Unsupervised hierarchical clustering of global gene expression data was broadly correlated with the clinical classification of NS. Differential gene expression (DGE) analysis of diseased groups (SSNS or SRNS) versus healthy controls identified 105 genes (58 up-regulated, 47 down-regulated) in SSNS and 139 genes (78 up-regulated, 61 down-regulated) in SRNS with 55 common to SSNS and SRNS, while the rest were unique (50 in SSNS, 84 genes in SRNS). Pathway analysis of the significant (P≤0.05, -1≤ log2 FC ≥1) differentially expressed genes identified the transforming growth factor-β and Janus kinase-signal transducer and activator of transcription pathways to be involved in both SSNS and SRNS. DGE analysis of SSNS versus SRNS identified 2,350 genes with values of P≤0.05, and a heatmap of corresponding expression values of these genes in each subject showed clear differences in SSNS and SRNS. Conclusion: Our study observations indicate that, although podocyte injury follows similar pathways in different clinical subgroups, the pathways are modulated differently as evidenced by the heatmap. Such transcriptome profiling with a larger cohort can stratify patients into intrinsic subtypes and provide insight into the molecular mechanisms of podocyte injury.

• 한국인터넷방송통신학회논문지
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• 제20권6호
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• pp.109-113
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• 2020

전 세계적으로 저탄소 녹색성장을 배경으로 인간과 자연이 공존하는 지속가능한 사회에 많은 이목이 쏠리고 있다. 탄소 배출은 에너지소비량에 따라 증가하게 되는데 전 세계적으로 건축물에 의한 에너지소비량이 무려 40%에 달한다. 우리나라에서는 2025년 신규 건축물에 한하여 제로 에너지 하우스를 의무화하는 계획을 하고 있으나 제로 에너지 하우스에 관한 기술을 민간 기술 투자에만 의지하고 있다. 정확한 로드맵이 확정된 것이 없어 현재 기술로는 일반건축물 보다 1.5배 이상의 비용이 들고, 민간 기업들은 실험용 주택 말고는 실제 거주하는 제로 에너지 하우스단지가 많이 없다는 것이 현실이다. 본 연구는 제로 에너지 하우스에 에너지를 효율적으로 관찰 및 제어하는 IOT 시스템을 도입하여 쾌적한 환경에 대한 모니터링이 웹에서 확인 및 거주자의 적정온도 유지시스템을 도입하여 제로 에너지 하우스의 새로운 모델을 연구하고자 한다.

• Korean Journal of Acupuncture
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• 제38권2호
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• pp.62-74
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• 2021

Objectives : The aim of this study is to analyze the effect of acupuncture and the details of acupuncture treatment methods on skin wrinkles. Methods : Search was conducted in Pubmed, KISS, and NDSL databases for acupuncture studies on skin wrinkles. The detailed therapeutic techniques of acupuncture used for skin wrinkles and the effect of acupuncture on skin wrinkle improvement were analyzed. Then, the study results using the same indicator were compared through meta-analysis in order to compare the effects of acupuncture with the control group. The quality of randomized controlled trials (RCTs) was assessed using the risk of bias (ROB) assessment tool (Ver. 1.0, Cochrane Collaboration). Results : A total of 10 RCTs and 19 case series were included in this study. The most frequently used therapeutic technique for skin wrinkle improvement was Microneedle therapy system (MTS, n=19), and they were mostly used with aesthetic solutions (13 out of 19 studies). Skin wrinkle related indexes were most commonly used to evaluate skin wrinkle improvement (n=14). Panax Ginseng pharmacopuncture showed the most remarkable effect in improving the range of skin wrinkles and the depth of skin wrinkles. MTS+epidermal growth factor improved the levels of skin hydration, skin elasticity, and variation of skin pigment the most. MTS+Endothelial precursor cell improved the number of skin melanin the most. Three RCTs included in the meta-analysis showed moderate ROB on average. Conclusions : Acupuncture treatment was effective in improving skin wrinkles. However, a precise study design using unified indicators and appropriate controls needs to be performed in further studies in order to establish more convincing evidence of the effectiveness of acupuncture for improving skin wrinkles.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권5호
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• pp.1909-1928
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• 2021

At present, the Colombian government shares information on threats or vulnerabilities in the area of cybersecurity and cyberdefense, from other government agencies or departments, on an ad-hoc basis but not in real time, with the surveillance entities of the Government of the Republic of Colombia such as the Joint Command of Cybernetic Operations (CCOCI) and the Cybernetic Emergencies Response Team of Colombia (ColCERT). This research presents the MS-CSIRT (Management System Computer Security Incident Response Teams) methodology, that is used to unify the guidelines of a CSIRT towards a joint communication command in cybersecurity for the surveillance of Information Technology (IT), Technological Operations (TO), Internet Connection Sharing (ICS) or Internet of Things (IoT) infrastructures. This methodology evaluates the level of maturity, by means of a roadmap, to establish a CSIRT as a reference framework for government entities and as a guide for the areas of information security, IT and TO to strengthen the growth of the industry 4.0. This allows the organizations to draw a line of cybersecurity policy with scope, objectives, controls, metrics, procedures and use cases for the correct coordination between ColCERT and CCOCI, as support entities in cybersecurity, and the different companies (ICS, IoT, gas and energy, mining, maritime, agro-industrial, among others) or government agencies that use this methodology.

• 한국의류학회지
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• 제45권1호
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• pp.28-45
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• 2021

The importance of smart clothing as a product is increasingly emphasized as further growth in the potential of the smart market is expected. There is a high understanding and sympathy for the potential of smart clothing in the mass consumer market; therefore, commercialization is not actively carried out. This study enhances the understanding of the development direction of products with a focus on technical benefits, in order for smart clothing to gain access to customers as wearable devices. This study identifies major technologies used in smart clothing through an analysis of the patent technology status of smart clothing in Korea. Smart clothing is divided into three types: passive smart, active smart and advanced smart clothing based on a reaction mechanism and functional scope. We present the smart clothing and discuss the product features for three types. According to research, smart clothing products were equipped with passive, active, and advanced smart systems as well as provided new services by converging big data and AI technologies, rather than only using technologies such as sensors, controls, and actuators. Future directions for new smart clothing product development is also discussed in the conclusion.