• Title/Summary/Keyword: growth and activities

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Determination of Types and Element on Parking Ramp (주차장 램프 형식 결정 및 제원 산정에 관한 연구)

  • Kwon, Sung-Dae;Kim, Yoon-Mi;Nam, Chang-Kyu;Ha, Tae-Jun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.5
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    • pp.2021-2031
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    • 2013
  • Due to the rapid economic growth within the nation, the quality of life of individuals have improved dramatically. The scope of living activities of individuals have also extended, resulting in a rapidly increasing demand for automobiles. The number of vehicles registered in Korea is rapidly increasing and will reach 188.71 million as of December 2012. Compared to the registered residents of 50.94 million provided by the Ministry of Public Administration and Security, the registered population reflects about 4 people per every automobile. Due to the high demands for vehicles, the demands for parking lots in collective housing and businesses are also increasing. In reality, the current state of expansion of parking lots are underground, due to the limited available space on ground level. Specifically, the slope of a parking lot cannot exceed 17% linear slope and 14% curved slope according to the 'parking lot laws', however studies show that the driver feels at risk for safety when stopped on the parking ramp while driving in the parking lot. This study seeks to examine the suitability of parking lot ramps, concerning the safety aspects of the driver. First, the ramp type was categorized as linear or curved, then test drives were performed based on variations of slopes, slant distances, directions and points. A survey was administered to the driver after the completion of the test drive, in order to element design for an ideal ramp. In the case of curved ramp, the results of the estimate suggests a counterclockwise, slope at a maximum of 12% incline. The maximum slope for a linear ramp was analyzed to be between 13~14%, suggesting that slope greater than 15% need to be eliminated. In conclusion, it is anticipated that the element design parking ramp reported in this study will help to serve as a reference for future parking lot related guidelines, and provide cost effective traffic safety mechanisms in future parking lot businesses to follow.

Anti-inflammation Activities of Cultured Products from Suspension Culture of Aloe vera Callus (Aloe vera Callus 현탁배양 생성물의 항염증 활성)

  • Kim, Myung Uk;Cho, Young Je;Lee, Shin Young
    • Journal of Applied Biological Chemistry
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    • v.56 no.3
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    • pp.157-163
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    • 2013
  • Cultured products (callus and exopolysaccharide) were obtained from suspension culture of Aloe vera callus, and the extracts of callus were further prepared with cold water or 60% ethanol solution. The ethanol extract of callus (AC) and exopolysaccharide (ACP) of 10 mg/mL exhibited the relatively higher suppression activity of 43.2-52.1% against hyaluronidase activity. Thus, their anti-inflammatory effects were further investigated using animal cell (Raw 264.7) in vitro. Though AC shows a slight suppression effect of cell survival rate (97%) using MTT assay in the presence of $400{\mu}g/mL$ AC- dimethyl sulfoxide (DMSO), cell growth promotion was observed in the other samples of lower levels. It indicates that the ethanol extract of Aloe callus rarely affect cell survival rate in the ranges ($200-400{\mu}g/mL$) used in the study. Using Griess reagent, the suppression of NO production by the aloe callus extract was analyzed by measuring the amount of the nitrite produced in Raw 264.7 culture activated by lipopolysaccharide (LPS). As a result, supplementation of AC-distilled water (DW) and AC-DMSO produced higher levels of NO than the positive control LPS. However, the NO suppression effect by ACP-DW was so intense that lower amount ($80-100{\mu}g/mL$) suppressed NO production to the level of the control. The effect was attributed to the expression of the iNOS. Then, Raw 264.7 cells were stimulated with the LPS and expression of COX-2 protein level was analyzed depending on the Aloe suspension culture product treatment. The results showed that the ACP-DW supplemented medium did not express COX-2 by itself, and LPS stimulated COX-2 expression was slightly decreased. On the other hand, realtime-PCR analysis of the expression of inflammatory cytokine showed that IL-$1{\beta}$ and TNF-${\alpha}$ expression was highly suppressed in the ACP- distilled water supplemented medium.

Amylase Activity and Characterization of Microorganism Isolated from in Aquacultural Effluents Sediment Layer (양식장 배출수 퇴적층에서 분리된 미생물의 다당분해효소 활성 및 특성)

  • Kim, Man-Chul;Jang, Tae-Won;Harikrishnan, Ramasamy;Moon, Young-Gun;Song, Chang-Young;Kim, Gi-Young;Heo, Moon-Soo
    • Journal of Life Science
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    • v.19 no.3
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    • pp.366-372
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    • 2009
  • In the course of screening of useful enzyme-producing microorganisms from marine sedimentary layers, we isolated 2 amylase producing strains and tested their amylase producing activities. Analyses of 16S rDNA sequences and biochemical methods (BIOLOG) of two isolates showed that they were confirmed to be a gram positive Bacillus sp. and gram negative Pseudoalteromonas sp., respectively. Excellent amylase producing strains were termed Bacillus sp. ST-63 and Pseudoalteromonas sp. ST-140, and further studies were conducted on their amylase producing characteristics. Optimum conditions for cell growth in amylase activity were obtained when the isolate (Bacillus sp. ST-63 and Pseudoalteromonas sp. ST-140) was cultured at $30^{\circ}C$ and pH $7{\sim}8$.

Legal Review on the Regulatory Measures of the European Union on Aircraft Emission (구주연합의 항공기 배출 규제 조치의 국제법적 고찰)

  • Park, Won-Hwa
    • The Korean Journal of Air & Space Law and Policy
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    • v.25 no.1
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    • pp.3-26
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    • 2010
  • The European Union(EU) has recently introduced its Directive 2008/101/EC to include aviation in the EU ETS(emissions trading system). As an amendment to Directive 2003/87/EC that regulates reduction of the green house gas(GHG) emissions in Europe in preparation for the Kyoto Protocol, 1997, it obliges both EU and non-EU airline operators to reduce the emission of the carbon dioxide(CO2) significantly in the year 2012 and thereafter from the level they made in 2004 to 2006. Emission allowances allowed free of charge for each airline operator is 97% in the first year 2012 and 95% from 2013 and thereafter from the average annual emissions during historical years 2004 to 2006. Taking into account the rapid growth of air traffic, i.e. 5% in recent years, airlines operating to EU have to reduce their emissions by about 30% in order to meet the requirements of the EU Directive, if not buy the emissions right in the emissions trading market. However, buying quantity is limited to 15% in the year 2012 subject to possible increase from the year 2013. Apart from the hard burden of the airline operators, in particular of those from non-European countries, which is not concern of this paper, the EU Directive has certain legal problems. First, while the Kyoto Protocol of universal application is binding on the Annex I countries of the Climate Change Convention, i.e. developed countries including all Member States of the European Union to reduce GHG at least by 5% in the implementation period from 2008 to 2012 over the 1990 level, non-Annex I countries which are not bound by the Kyoto Protocol see their airlines subjected to aircraft emissions reductions scheme of EU when operating to EU. This is against the provisions of the Kyoto Protocol dealing with the emissions of GHG including CO2, target of the EU Directive. While the Kyoto Protocol mandates ICAO to set up a worldwide scheme for aircraft emissions to contribute to stabilizing GHG concentrations in the atmosphere at a level that would prevent dangerous anthropogenic interference with the climate system, the EU ETS was drawn up outside the framework of the international Civil Aviation Organization(ICAO). Second, EU Directive 2008/101 defines 'aviation activities' as covering 'flights which depart from or arrive in the territory of a Member State to which the [EU] Treaty applies'. While the EU airlines are certainly subject to the EU regulations, obliging non-EU airlines to reduce their emissions even if the emissions are produced during the flight over the high seas and the airspace of the third countries is problematic. The point is whether the EU Directive can be legally applied to extra-territorial behavior of non-EU entities. Third, the EU Directive prescribes 2012 as the first year for implementation. However, the year 2012 is the last year of implementation of the Kyoto Protocol for Annex I countries including members of EU to reduce GHG including the emissions of CO2 coming out from domestic airlines operation. Consequently, EU airlines were already on the reduction scheme of CO2 emissions as long as their domestic operations are concerned from 2008 until the year 2012. But with the implementation of Directive 2008/101 from 2012 for all the airlines, regardless of the status of the country Annex I or not where they are registered, the EU airlines are no longer at the disadvantage compared with the airlines of non-Annex I countries. This unexpected premium for the EU airlines may result in a derogation of the Kyoto Protocol at least for the year 2012. Lastly, as a conclusion, the author shed light briefly on how the Korean aviation authorities are dealing with the EU restrictive measures.

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Mineralogical Analysis of Calcium Silicate Cement according to the Mixing Rate of Waste Concrete Powder (폐콘크리트 미분말 치환율에 따른 이산화탄소 반응경화 시멘트의 광물상 분석)

  • Lee, Hyang-Sun;Song, Hun
    • Journal of the Korea Institute of Building Construction
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    • v.24 no.2
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    • pp.181-191
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    • 2024
  • In the realm of cement manufacturing, concerted efforts are underway to mitigate the emission of greenhouse gases. A significant portion, approximately 60%, of these emissions during the cement clinker sintering process is attributed to the decarbonation of limestone, which serves as a fundamental ingredient in cement production. Prompted by these environmental concerns, there is an active pursuit of alternative technologies and admixtures for cement that can substitute for limestone. Concurrently, initiatives are being explored to harness technology within the cement industry for the capture of carbon dioxide from industrial emissions, facilitating its conversion into carbonate minerals via chemical processes. Parallel to these technological advances, economic growth has precipitated a surge in construction activities, culminating in a steady escalation of construction waste, notably waste concrete. This study is anchored in the innovative production of calcium silicate cement clinkers, utilizing finely powdered waste concrete, followed by a thorough analysis of their mineral phases. Through X-ray diffraction(XRD) analysis, it was observed that increasing the substitution level of waste concrete powder and the molar ratio of SiO2 to (CaO+SiO2) leads to a decrease in Belite and γ-Belite, whereas minerals associated with carbonation, such as wollastonite and rankinite, exhibited an upsurge. Furthermore, the formation of gehlenite in cement clinkers, especially at higher substitution levels of waste concrete powder and the aforementioned molar ratio, is attributed to a synthetic reaction with Al2O3 present in the waste concrete powder. Analysis of free-CaO content revealed a decrement with increasing substitution rate of waste concrete powder and the molar ratio of SiO2/(CaO+SiO2). The outcomes of this study substantiate the viability of fabricating calcium silicate cement clinkers employing waste concrete powder.

The Effects of Indole Acetic Acid (IAA) on Cd$^{2+}$_induced Physiological Toxicities in Commelina communis L. (Cd$^{2+}$ 에 의한 닭의장풀의 식물 독성에 Indole acetic acid가 미치는 영향)

  • 이준상
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.234-239
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    • 2003
  • 3-weeks old Commelina was transferred to and grown in Hoagland solution (($\pm 100 \mu M \;Cd^{2+}, 100 \mu M \;Cd^{2+}+10 \mu M\;IAA,100 \mu M\; Cd^{2+}+100 \mu M \; IAA,100 \mu M \; Cd^{2+} +1 \mu M \; IAA)$) for three weeks and then a number of physiological activities was investigated. In the control the length of stem was increased to 7 cm after 3 weeks, but in the treatment of $Cd^{2+}$, 2.0 cm was grown. In cases of IAA with $Cd^{2+}$, the growth of the plants was increased to 3.7 cm, 5.0 cm and 3.3 cm in 100 $\mu$M, 10 $\mu$M and 1 mM IAA respectively, Cadmium stimulated stomatal opening. The stomata, treated with $Cd^{2+}$ opened to n degree of about 6.1 $\mu\textrm{m}$, but the stomata, treated with no cadmium opened to 4.5 $\mu\textrm{m}$. In the treatment of various concentation of IAA with cadmium the stomata opend to about 1 $\mu\textrm{m}$ more than that of cadmium alone. Cadmium reduced total chlorophyll content up to 13% for 3 weeks. In the treatment of $Cd^{2+}$ + IAA (10 $\mu$M and 100 $\mu$M) the clear change of total chlorophyll content was not observed, but in the addition of 1 mM IAA to $Cd^{2+}$ reduced the total chlorophyll content to about 9%. $Cd^{2+}$ reduced to 9% and 11% of Fv/Fm after two and three weeks respectively. In cases of IAA with $Cd^{2+}$, there were no clear changes of Fv/Fm. Cadmium reduced water potential to 67% after 3 weeks incubation, but in cases of IAA with $Cd^{2+}$, water potential was not clearly changed. Therefore, it could be concluded that the treatment of IAA showed clear alleviation of $Cd^{2+}$-induced several physiological toxicities.

Simultaneous Application of Chemicals and Temperature for the Effective Control of Trouble Seaweed Ulva australis (구멍갈파래(Ulva australis) 생육제어 효과 증진을 위한 화합물 및 고온의 복합처리)

  • Kim, Jin-Seog;Kim, Bo Gwan;Kwak, Hwa Sook
    • Weed & Turfgrass Science
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    • v.7 no.1
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    • pp.35-45
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    • 2018
  • The seaweed Ulva spp., which is frequently bloomed in coastal areas, have negatively affected on marine ecosystem and industrial activities. Therefore, many researches have been conducted to solve this problem in the worldwide. In this study, we carried out several experiments to develop the methods for effectively controlling Ulva growth through an alone or mixture application of chemical and temperature. Three chemical mixtures ($H_2O_2$+N-vanillylnonanamide; $H_2O_2$+nonanoic acid; $H_2O_2$+sodium citrate), those had a synergistic effect to the death of Ulva australis (ULAUS), were found out. On the other hand, the death of ULAUS was significantly enhanced and accelerated as some chemicals were briefly treated with warm water of $40^{\circ}C$ rather than $25^{\circ}C$, showing that peracetic acid 100 ppm, sodium percarbonate 100 ppm, and hydrogen peroxide 30 ppm has a better activity than that of sodium chlorite 200 ppm and menadione sodium bisulfite 4 ppm. In addition, a strong synergistic effect to the death of ULAUS thallus was also observed when the sodium citrate 1,000 ppm (pH 3.0) or acetic acid 200 ppm (pH 3.5) solution prepared in f/2 medium were treated in a short time at $40^{\circ}C$. However, an additive effect was only appeared as pH values of their solutions were increased to 8.0. Taken together, It seemed that our results could be developed as one of an eco-friendly practical measures useful for alleviating Ulva bloom in the future.

Study on the Effects of R&D Activities on the Exports of Korean Economy (R&D투자가 한국경제 수출에 미치는 영향 분석)

  • Kim Byung-Woo
    • Journal of Technology Innovation
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    • v.14 no.1
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    • pp.31-66
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    • 2006
  • The country with a relative abundance of human capital conducts relatively more R&D in the steady state than its partner. This country acquires the know-how to produce a relatively wider range of innovative goods. High technology comprises a large share of the national economy in the human-capital rich country and real output growth is faster. This prediction would seem to accord weakly with empirical observation of Korean economy.

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Immunological Activity of Bovine Colostral Whey Protein Containing TGF-β from Imsil Province (임실지역 젖소 초유로부터 분리한 TGF-β 함유 유청 단백질의 면역활성)

  • Yang, Hee-Sun;Oh, Hyun-Hee;Choi, Hee-Young;Park, Jong-Hyuk;Kim, Kyoung-Hee;Oh, Jeon-Hui;Jung, Hoo-Kil
    • Food Science of Animal Resources
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    • v.32 no.3
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    • pp.339-345
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    • 2012
  • This experiment was carried out in order to separate bovine colostral whey protein from Imsil province and to test the effect of immunological activity on RAW 264.7 cells. The colostral whey protein contained TGF-${\beta}$ 7, 475 pg/g in total. We first tested the effect of the colostral whey protein on the proliferation of RAW 264.7 cells and it demonstrated cytotoxicity at concentrations greater than 20 mg/mL. Therefore, the immunological activities of colostral whey protein were investigated in maximum concentration of 10 mg/mL on LPS-induced RAW 264.7 cells. Results indicated that colostral whey protein inhibited the LPS-induced nitric oxide (NO) production in a dose-dependent manner. The colostral whey protein also suppressed the productions of proinflammatory cytokines (TNF-${\alpha}$, IL-$1{\beta}$, IL-6) in a dose-dependent manner. In addition to the immunological activity, colostral whey protein led to the expression of heme oxygenase-1 (HO-1) in RAW 264.7 cells. In conclusion, colostral whey protein containing TGF-${\beta}$ inhibited the production of NO, TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 via expression of HO-1.

The Standing Crops and Soil-borne Microfungal Flora of Phyllostachys reticulata in Korea (한국산(韓國産) 왕대나무의 현존량(現存量)과 토양(土壤) 미세균류상(微細菌類相))

  • Kim, Kwan-Soo
    • The Korean Journal of Mycology
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    • v.7 no.2
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    • pp.91-116
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    • 1979
  • This paper is to investigate the standing crops and microfungal flora in soil in Phyllostachys reticulata forests in both the Yesan area (A) and the Kwangsan area (B). The stand density of the bamboo revealed 17,250 shoots per ha in area A, and in area B 14,780 shoots which were 16.1% less in number than area A. In respect to the environmental factors between the two areas, the mean temperature during the growth period was $1.5{\sim}2^{\circ}C$ higher in area B than in area A, soil tempeature also was $1{\sim}2^{\circ}C$ higher in area B, and the total quantities of nitrogen, phosphoric acid and organic compounds contained in the soil of area B were also slightly higher than those of area A. In area B the quantities of dried leaf matter, humus, and vegetation in the bamboo forest were also larger than in area A. In addition, five more species of microfungi which playa role in the decomposition of the various organic materials in the bamboo forests were identified in area B: Mortierella elongata, Mucor circinelloides, Aspergillus japonicus, Penicillium waksmani and Trichoderma lignorum. The atmospheric temperature in the inner portions of the bamboo forests was lower than the outside temperature, but the humidity was higher. The rates of relative illuminance were measured in area A at 4.19%, and in area B at 2.7%. These values revealed that the photosynthetic acitivity in the lower part of the bamboo was lost but it was considered that lower illuminance increased the microfungal activities in the vicinity of the surface soil. Since the productive structure of the bamboo showed that the maximum amount of photosynthesis was located in the upper portion of the bamboo in area B, it was considered to be an effective structure in maintaining the high productivity of the bamboo. The allometric relation between $D^2H$ and dry weight of stems(Ws), branches(Wb) and leaves(Wl) of the bamboo in area A were appoximated by log Ws=0.5262 log $D^2H$+1.9546; log Wb=0.6288 log $D^2H$+1.5723; log Wl=0.5181 log $D^2H$+1.8732, and those of the bamboo in area B were approximated by log Ws=0.5433 log $D^2H$+1.8610; log Wb=0.1630 log $D^2H$+2.3475; log Wl=0.4509 log $D^2H$+2.0041. From the above, the standing crops in area A were measured thus: Ws was 1,128. 83kg; Wb, 689.05kg; Wl, 926.69kg and Wl, 2,744.57kg per 10a. In area B, Ws was 1,206. 66kg; Wb, 679.92kg; Wl, 1,112.51kg and Wt, 2.999kg per l0a. Significant differences from the result of t-test were for $D^2H$ Ws, Wl and Wt between areas A and B. But no significant difference was found for Wb. In order to record as completely as possible the microfungal flora of the areas, every possible means was tried, and 158 strains of fungi were isolated, and of these, the microfungi of 55 species were identified. The dominant species were Trichoderma viride, Penicillium janthinellum, P. commune, Aspergillus oryzae, A. niger, A. gigantus, A. fumigatus, Mortierella ramaniana, var. anguliFPora, Mucor hiemalis and Zygorhynchus moelleri. According to the above results, it was revealed that optimum soil, the increases of soil materials, more species of soil microfungi, and the atmospheric temperature during the growth period have made the bamboo flourish and bring more species and larger quantities of vegetation in the bamboo forests. The correlation between the standing crops and environmental factors in the bamboo forest is considered to be a complicated relationship of all the factors, but the stand density is thought to be the most important factor involved.

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