• Title/Summary/Keyword: growing temperature

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Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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Species Composition of Fish Assemblage in Eelgrass Bed of Bongam on Hansando Island, Korea (경남 한산도 봉암 잘피밭 어류의 월별 종조성 변화)

  • Han, Dong-Hun;Lee, Dae-Hee;Park, Jun-Su;Kim, Jun-Sop;Lee, Yong-Deuk;Park, Jong-Yul;Gwak, Woo-Seok
    • Korean Journal of Ichthyology
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    • v.29 no.2
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    • pp.130-138
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    • 2017
  • Fish species composition in eelgrass bed of Bongam on Hansando island, Korea was determined using monthly samples collected by a surf net from August 2009 to July 2010. A total of 30 species, 3,237 individuals and 6,850.1g of fish were collected. The dominant species were Gymnogobius heptacanthus, Rudarius ercodes, Takifugu niphobles, Chaenogobius annularis, Pterogobius elapoides and Syngnathus schlegeli which accounted for 86.7% of total number of individuals of fish collected. Eelgrass started growing from May ($DW61.9g/m^2$) showing a peak biomass in August ($DW81.6g/m^2$), and low values in November ($DW57.23g/m^2$) to February ($DW15.41g/m^2$). The fish assemblages in the eelgrass beds showed clear seasonal changes; the number of fish species and individuals were low from September to December 2009, and high from December 2009 to July 2010. Seasonal variation in species composition and abundance was related to the water temperature and standing crops of eelgrass.

Studies on the Effects of Radiation from Radioisotopes incorporated into Plants(Ⅵ) Effects of P-32 incorporated into Seeds on the Growth of Flax (작물에 흡수된 방사성 동위원소의 내부 조사효과에 관한 연구(Ⅵ) 아마의 생장에 미치는 P-32의 영향)

  • 손은용
    • Journal of Plant Biology
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    • v.12 no.1
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    • pp.22-30
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    • 1969
  • To investigate the effects of internal radiation of various intensityon the test crop(flax), seeds of four levels of activity($15.5{\times}10-4 $\mu$c/grain, 9.0{\times}10-2$\mu$c/grain, 2.2{\times}100$\mu$c/grain and 3.8{\times}10-1$\mu$c/grain)$ which had been obtained by immersing them into various concentrations of P-32 original solution (total activity: 90 mc, To: 3/21) for 24 hours at room temperature, were germinated, transplanted later into pots, and the rate of germination and the successive growth were observed, and the inorganic conents of the plant top were analyzed. The results are as follows: 1. At the early stage of germination, the plants manifested themselves both inhibitory and promotive effects at higher and lower activity levels respectively, compared those of the control. These difference of growth on account of different levels of activity appeared, however, to be gradually narrowed in the course of time after germination, except at the highest activity. 2. Two weeks after transplanting, the plants of the lowest activity showed more vigorous growth than those of control. The plants belonging to the other activity levels on the other hand, tended to be less growing, the higher the activity. However, this growth gap between treatments seemed to be progressively closed one month after transplanting. 3. Most of the leaves and stems of the plants belonging to the highest activity level ($3.8{\times}101$\mu$c/grain$) were withered during the early stage of growth, and this damage did not recover. 4. Practically no difference of growth was observed among treatments(excluding that of highest activity) one and half months after transplanting. 5. The fluorescence tended to be mroe delayed than the control, as the activity decreased. 6. There was a tendency that the number of pods harvested were larger in the plants treated with P-32 than that of the control. 7. The proportion of fiber in the plants at harvest appeared to be larger at lower activity and smaller at higher P-32 concentration than that of control. 8. As for the inorganic contents of the plant top harvested the floowing tendency was observed: (1) Nitrogen content was highest at the highest activity level at which the poorest growth ensued. (2) There was no clear difference of phosphorus content among treatments. (3) The contents of potassium and magnesium were higher than control at the medium levels of activity. (4) Calcium content of all treated blocks was found to be more than that of control.

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A Study on Creep Effect of Synthetic Fiber Rope Mooring System on Motion Response of Vessel and Tension of Mooring Line (섬유로프 계류시스템의 크리프 효과가 부유체의 운동응답 및 계류선의 장력 변화에 미치는 영향에 관한 연구)

  • Park, Sung Min;Lee, Seung Jae;Kang, Soo Won
    • Journal of the Society of Naval Architects of Korea
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    • v.54 no.2
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    • pp.151-160
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    • 2017
  • Growing demand and rapid development of the synthetic fiber rope in mooring system have taken place since it has been used in deep water platform lately. Unlike a chain mooring, synthetic fiber rope composed of lightweight materials such as Polyester(polyethylene terephthalate), HMPE(high modulus polyethylene) and Aramid(aromatic polyamide). Non-linear stiffness and another failure mode are distinct characteristics of synthetic fiber rope when compared to mooring chain. When these ropes are exposed to environmental load for a long time, the length of rope will be increased permanently. This is called 'the creep phenomenon'. Due to the phenomenon, The initial characteristics of mooring systems would be changed because the length and stiffness of the rope have been changed as time goes on. The changed characteristics of fiber rope cause different mooring tension and vessel offset compared to the initial design condition. Commercial mooring analysis software that widely used in industries is unable to take into account this phenomenon automatically. Even though the American Petroleum Institute (API) or other classification rules present some standard or criteria with respect to length and stiffness of a mooring line, simulation guide considers the mechanical properties that is not mentioned in such rules. In this paper, the effect of creep phenomenon in the fiber rope mooring system under specific environment condition is investigated. Desiged mooring system for a Mobile Offshore Drilling Unit(MODU) with HMPE rope which has the highest creep is analyzed in a time domain in order to investigate the effects creep phenomenon to vessel offset and mooring tension. We have developed a new procedure to an analysis of mooring system reflecting the creep phenomenon and it is validated through a time domain simulation using non-linear mooring analysis software, OrcaFlex. The result shows that the creep phenomenon should be considered in analysis procedure because it affects the length and stiffness of synthetic fiber rope in case of high water temperature and permanent mooring system.

Induction of Autolysis and Autoplast Formation of Anaerobic Clostridium thermohydrosulfuricum (혐기성 Clostridium thermohydrosulfuricum의 Autolysis 및 Autoplast 형성유도)

  • 김욱한;박동찬;정기택;이용현
    • Korean Journal of Microbiology
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    • v.27 no.4
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    • pp.357-365
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    • 1989
  • Induction conditions for autolysis and autoplast formation of thermophilic Clostridium thermohydrosulfuricum were studied. The cells in the initial exponential growth phase were well autolysed in Tris-HCl buffer or inorganic buffers containing univalents, such as $K^{+}$ and $Na^{+}$ , and chemicals such as cysteine-HCl, sorbitol and glycerol. Meanwhile, autolysis induction was slightly inhibited by divalents, such as $Mg^{2+}, Mn^{2+}, Ca^{2+}, Ni^{2+}$, and strongly by divalents, such as $Fe^{2+}, Cu^{2+}$ and citric acid. The autolysis was stimulated when the cells were grown in the medium containing ampicillin that inhibites cell wall synthesis, meanwhile, it was slightly inhibited by nucleic acids and protein synthesis inhibitors. The optimal pH and temperature for the induction of autolysis were 7.5 and $60^{\circ}C$, respectively. On the other hand, the cells were autoplasted without lysozyme treatment during autolysis due to the stabilization of protoplasmic membrane in the presence of divalents such as $Mg^{2+}, Mn^{2+}, Ca^{2+}, Ni^{2+}$. Autoplast formation was mostly induced at $37^{\circ}C$ in 50mM Tris-HCl buffer (pH 7.5) containing 20 mM $MgCl^{2}$ and 0.3 M glycerol, and in the late exponential growth phase growing cell.

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Observation, Experiment, and Analysis of the Ice Spikes Formation (솟는 고드름의 형성과정에 관한 관찰, 실험 및 분석)

  • Yoon, Ma-Byong;Kim, Hee-Soo;Son, Jeong-Ho;Yang, Jeong-Woo
    • Journal of the Korean earth science society
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    • v.30 no.4
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    • pp.454-463
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    • 2009
  • In this study, from January 2006 to February 2009, we observed 107 ice spikes formed in a natural state, and analyzed their environment. We developed an experimental device to reproduce ice spikes in laboratory and successfully made 531 ice spikes. We analyzed the process of the formation and the principle of how those ice spikes grow through videotaped data of the formation in the experiment. In the natural world, when the surface of water and the lower part of a vessel begin to freeze, a vent (breathing hole) develops at the surface where an ice is not frozen; this vent serves as the seed of an ice spike. It is assumed that the volume expansion of ice in the vessel which occurs when water freezes makes the supercooled water go upward through the vent and becomes an ice bar called an ice spike. In the laboratory, however, when distilled water is poured into an ice tray cube and kept in the experimental device for about one and a half hours at a temperature of -12- $-13^{\circ}C$, a thin layer of ice then begins to develop on the surface of the water, the vent is formed, and ice spikes form for about 10-30 minutes. These spikes stop growing when the end becomes clogged. Ice spikes can be described as falling into seven categories of shape, with the apex type topping the list followed by the slant type in the natural state and the vertical type predominating in the laboratory.

A Case Study on the Creating Artificial Planting Ground on the Waste Landfill Sites -In Case of the Bank Isolated Section Planting Layer at the Landfills of Satellite Cities of Seoul- (폐기물매립지 인공식재지반 조성 사례연구 -수도권매립지 제방이격구간 식재층을 대상으로-)

  • 조주형;이재근
    • Journal of the Korean Institute of Landscape Architecture
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    • v.29 no.1
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    • pp.131-139
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    • 2001
  • This paper aims at surveying through case studies the planting possibility on the interval artificial ground between the bank and the core landfill of the first section of works in the SUDOKWON Landfill area landfill area which was completed, followed by the layer-on-layer landfill process involving the latch or sealing layer against emitting landfill gas from the reclaimed waste. The survey results are as follows; 1. The layers of the artificial planting ground on the landfill were established on the basis of top-on-top procedure for a waste layer, a topping soil layer (T=50cm), a gas blocking layer (broken stones T=30cm), a filter layer (non-woven fabric 700g), a sheet protecting soil layer (T=20cm), and a blocking layer (HDPE SHEET 2.0mm), an irrigation layer (SAND T=30cm), a filter layer (non-woven fabric 700g), a sheet protecting soil layer (T=20cm), and a blocking layer (HDPE SHEET 2.0mm), an irrigation layer (SAND T=30cm), a filter layer (non-woven fabric 700g), a planting layer (T=90cm+), a top mound (T=2m). 2. Since no direct damage on the planting layer affected by the landfill gas was detected, planting is found to be still possible and successful except the severely unequal subsidence portion. 3. The mortality rate is discovered different on different trees: Pinus thunbergii (H3.0$\times$W1.0m) 11.25%, Pinus thunbergii (H2.5$\times$W0.8m) 4.73%, Koelreuteira paniculata 8.67%, Hibiscus syriacus 5.68%, Deutzia parviflora 6.50%, Forsythia koreana 8.17%, Rho. yedoense v. poukhanese 32.22%, and Spiraea pru v. symplicifolia 18.89%; although the last two of which are generally considered to have a strong generic growing character, they are subject to be weakened when exposed to the contaminated microclimate of the site like landfill gas. 4. The damage rates, on Pinus thunbergii, Koelreuteria paniculata, Hibiscus syracus, Forsythia koreana, Deutzia parviflora, Rho. yedoense v. poukhanense were shown to decrease to 7.31-17.69% in the second check (June 2000) lower than 5.77-46.92% in the first examination (June 1999), whereas the damage on Spiraea pru v. symplicifolia relatively increased. It is believed that preparatory method of the air pollution, change of temperature, odor by emitting landfill gas, and minute dust from vehicles should be made, and a research on this matter will be conducted in the near future.

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Varietal Variation of Grain-Filling Period under the Different Environmental Conditions in Barley (대맥의 재배환경에 따른 등숙일수의 품종간 변이)

  • Hong-Suk Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.202-210
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    • 1983
  • These experiments were conducted to study the relationship among agronomic traits, varietal variation of grain-filling periods and the effect of environmental conditions on the grain-filling in the ten selected barley cultivars. The varietal variation of grain-filling periods was of significance: Tokak had the similar increment in both greenhouse and field plot, Suweon two row was believed to be an cultivar with short grainfilling period. Cultivars with higher grain-filling rates tended to. have significantly lower ash content. Ash content of a cultivar tended to be lower when grown under favorable condition. At maturity, the time of development of yellow or dark pigment at the crease base could be a possible criterion for physiological maturity determination. However, this requires further study. Average grain-filling period in the different conditions was constant, but varietal differences were 9 days in the greenhouse and 5 days in the field. The grain moisture content was not constant in accordance to both cultivars and growing conditions. Average accelerated days for heading and ripening periods were very constant in comparision with field and vinyl mulching suggests that the selected materials will be useful for genetic study on grain-filling periods. The cultivars and grown conditions with higher accumulated temperature per $100^{\circ}C$ from early grain-filling had larger grain-filling rates and heavier 1000 grain weight. This study showed that if the variation among and within cultivars is to be minimized, the greenhouse and vinyl mulching plots could be useful for genetic study on grain-filling periods in $BC_1$ and $F_2$ segregation.

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Characteristics of Reflective Light over Red and Black Plastic Mulch, and Effect on the Quality and Yield of the Oriental Melon and Tomato (적색 비닐멀칭과 흑색 비닐멀칭의 반사광 특성과 참외와 토마토의 품질과 수량에 미치는 영향)

  • Hong, Seung-Chang;Heo, Jeong-Wook;Lee, Jeong-Taek;Kang, Kee-Kyung
    • Korean Journal of Environmental Agriculture
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    • v.30 no.4
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    • pp.414-418
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    • 2011
  • BACKGROUND: Plastic mulches widely used in raised-bed culture mainly to conserve water, control weeds and raise soil temperature. The most widely used plastic mulch colour is black. Reflective red and far-red light can affect on growth and yield of various vegetable crops. Objectives of this study were to investigate the characteristics of reflective light of black and red plastic mulches, and to evaluate the reflective red and far-red light on the quality characteristics and yield of the Oriental melon (Cucumis. Melo L.) and tomato (Lycopersicon esculentum L.). METHODS AND RESULTS: Oriental melon and tomato were cultivated over the reflective red and black plastic mulches in plastic house. Reflected red and far-red light over the red plastic mulch were 2.6 times higher than those of black plastic mulch. Red to F-Red ratio of black plastic mulch, red plastic mulch and sunlight were 1.14, 0.93 and 1.16 respectively. Intensity of reflected red and far-red light over red plastic mulch were highest at surface height of 30 cm. The higher the height of the surface decrease the intensity of far-red light. Accordingly, Red to F-Red ratio were increased. Reflective red plastic mulch increased the weight of fruit and content of sugar in Oriental melon and tomato. CONCLUSION(s): Yield of Oriental melon over reflective red plastic mulch was higher than that of black plastic mulch. These results suggested that reflected red and far-red light over the red plastic mulch affected allocation of photosynthate in growing Oriental melon.

Oxidative Stress and Antioxidant Responses in Poplar Clones Irrigated with Livestock Waste Leachate (양돈폐수 처리에 의한 포플러클론의 산화스트레스와 항산화반응)

  • Je, Sun-Mi;Yeo, Jin-Gi;Woo, Su-Young
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.13 no.3
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    • pp.140-147
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    • 2011
  • We studied the influence of livestock waste leachate on oxidative damage and antioxidative responses in poplar clones in August which increase the demand of antioxidants because of high temperature and high light during this period. We measured ion leakage, antioxidant enzyme activities (APX, GR), and carotenoid contents. Oxidative damage and antioxidative responses by treated livestock waste leachate in poplar clones showed various results. We divided poplar clones into three groups using the criteria based on ion leakage which represent cell damage induced oxidative stress. Eco 28, 62-10, Bonghwa1 and Dorskamp belonged to the first group in which the cell damaged level was lower than that of the control. The results suggest that this group augmented for demand of antioxidative in summer because high concentration of nitrogen induced by treatment of live stock wastes acted as environmental stress. Consequently, they failed to keep up the homeostasis of reactive oxygen species. The second group in which the cell damaged level was similar to that of the control was Suwon, 72-30 and 72-31 clones. Finally, 97-18 clone belonged to the third group in which the cell damaged level was lower than that of the control group. In this case, nitrogen treated by livestock waste leakage decreased oxidative stress. 97-18 clone was the clones with the least damage in summer oxidative stresses treated by livestock waste leakage. These results suggest that the high concentration nitrogen due to the livestock waste leakage can act differently upon the clones. We speculate that the added oxidation damage in the summer (growing season) may have an effect on the total fresh weight and also influence the purification ability for livestock waste leakage. However, further studies are needed for the confirmation.