• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.217-223
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• 1993

To investigate the nature and location of the nisin-resistance determinant of Lactococcus lactis subsp. lactis 7962 (L. lactis 7962), a total plasmid DNA prepared from L. lactis 7962, a nisin producer, was used to transform L. lactis subsp. lactis LM0230, a plasmid-free and nisin-sensitive strain, by protoplast mediated transformation procedures. All of the nisin-resistant transformants acquired the ability to utilize sucrose at the same time, confirming the close linkage between these two determinants in L. lactis 7962. The plasmid DNA profiles of a few selected nisin-resistant transformants were examined by agarose gel electrophoresis. No common plasmid was found among the transformants and some small plasmids previously not present in L. lactis 7962 were detected. These transformants were named as L. lactis KL1, KL2, KL3, KL4, or KL5, respectively based on their plasmid profiles. Growth curves of all transformants were similar to that of L. lactis LM0230, but different from that of L. lactis 7962. L. lactis KL5 showed the highest level of resistance to nisin, growing up to 1, 200 IU nisin/ml after 40 hr incubation. Some nisin-sensitive derivatives of KL1 or KL2 were obtained by plasmid curing experiments. The plasmid DNA profiles of the nisin-sensitive KL1 derivatives were apparently the same as that of the KL1. All of the nisin-sensitive KL2 derivatives were plasmid-free, but a nisin-resistant strain with no apparent plasmid was also obtained. These results indicate that the nisin-resistance of the $Nis^r$ transformants is presumably mediated by the chromosomally located gene(s) rather than plasmid-encoded gene(s).

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.5
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• pp.31-40
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• 2017

The aim of this study is to estimate net irrigation requirements for red pepper during growing period using soil moisture model. The soil moisture model based on water balance approach simulates soil moisture contents of 4 soil layers in crop root zone considering soil moisture extraction pattern. The LAMP (Land-Atmosphere Modeling Package) high resolution meteorological data provided from National Center for AgroMeteorology (NCAM) was used to simulate soil moisture as the input weather data. Study area for the LAMP data and soil moisture simulation covers $36.92^{\circ}{\sim}37.40^{\circ}$ in latitude and $127.36^{\circ}{\sim}127.94^{\circ}$ in longitude. Soil moisture was monitored using FDR (Frequency Domain Reflectometry) sensors and the data were used to validate the simulation model from May 24 to October 20 in 2016. The results showed spatially detailed soil moisture pattern under different weather conditions and soil texture. Net irrigation requirements were also different by location reflecting the spatially distributed weather condition. The average of the requirements was 470.7 mm and averages about soil texture were 466.8 mm, 482.4 mm, 456.0 mm, 481.7 mm, and 465.6 mm for clay loam, sandy loam, silty clay loam, clay, and sand respectively. This study showed spatial differences of soil moisture and the irrigation requirements of red pepper about spatially uneven weather condition and soil texture. From the results, it was demonstrated that high resolution meteorological data could provide an opportunity of spatially different crop water requirement estimation during the irrigation management.

• Korean Journal of Pharmacognosy
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• v.26 no.2
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• pp.116-121
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• 1995

To seek for a stable supply source of taxol as an anticancer agent urgently needed for the treatment of ovarian cancer, the concentrations of taxol and cephalomannine in bark and needles of 37 yew (Taxus cuspidata S. et Z.) trees grown in 6 locations of Cheju Island were analyzed by using HPLC. A considerable variation in the content was observed in plant part and the location. The average taxol content in the needles ($220.3{\;}{\pm}{\;}56{\;}{\mu}g$ per g dry wt) was ca. 4 times hgher than that in the bark, whereas the content of cephalomannine in the needles $(353{\;}{\pm}{\;}141{\;}{\mu}g)$ was ca. 5 times higher than that in the bark. Particularly, even within Cheju Island, needles from trees grown near the coast including Sogwipo area contained much higher taxol contents than needles from trees grown at mountain locations, indicating that taxane level is affected by environmental effects. Taxol in the needles was confirmed by electrospray mass spectrometry. These results suggest that the needles of yew trees growing in Cheju Island may provide a renewable source for taxol.

• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.397-408
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• 2004

Due to the growing impact of non-point source pollution and limitation of water treatment technology, a new policy of water quality management, called a source protection, is now becoming more important in drinking water supply. The source protection means that the public agency purchases the pollution sensitive area, such as riparian zone, and prohibit locations of point and non-point sources. Many studies have reported that this new policy is more economical in drinking water supply than the conventional one. However, it is very difficult to determine location and size of the pollution sensitive zone in the watershed. In this paper, we presented the scientific criteria for the priority of the pollution sensitive zone, along with a case study of the upstream watershed of the Paldang Reservoir, Han River. This study includes applications of the analytical hierarchy process(AHP) and a watershed-based land prioritization(WLP) model. After major criteria affecting water quality were selected, the AHP and geographic analysis were performed. The WLP model allowed us to include both quantity and quality criteria, using AHP as the multi-criteria method in making decision and reflecting local characteristics and various needs. By adding a travel-time function, which represents the prototype effectively, the results secured adaptability and scientific objectivity as well. As such, the WLP model appeared to provide reasonable criteria in determining the prioritization of land acquisition. If the tested data are used with a validated travel-time and AHP method is applied after further discussion among experts in such field, highly reliable results can be obtained.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.30 no.6_1
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• pp.549-557
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• 2012

Spatial phenomena such as environment pollution, disease and the risk of spreading information need a rapid initial response to perceive spread event. Moving data perceive spread event through real-time processing and analysis. To process and analysis the event, spatial-temporal complex event processing is used. Previous spatialtemporal complex event processing is possible basis spatial operator but insufficient apply to design spatialtemporal complex event processing to perceive spatial phenomena of high complexity. This study proposed hierarchical spatio-temporal CEP design which will efficiently manage the fast growing incoming sensor data. The implementation of the proposed design is evaluated with GPS location data of moving vehicles which are used as the incoming data stream for identifying spatial events. The spatial component of existing CEP software engine has been extended during the implementation phase to broaden the capabilities of processing spatio-temporal events.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.2
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• pp.227-234
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• 2015

To obtain geographical range and growth-kinetics parameters of mottled sake (Beringraja pulchra) populations in the Yellow Sea, three mark-recapture experiments were carried out. Overall, 991 tagged individuals were released, and 4.1% of them were recaptured with the mean release period of 339 d (range, 8-1,420 d) and the mean growth rate of $1.4cm\;mon^{-1}$ (female, $1.5cm\;mon^{-1}$; male $1.3cm\;mon^{-1}$). In the first experiment, 667 individuals were released at Heuksan Island from April to June, 2007-2009, and 30 individuals were recaptured mainly at the north and the north-east coasts of the island, indicating absence of migration to the south of the island. In the second experiment, 323 individuals were released at several fishing grounds scattered in the Yellow Sea in 2010-2013, and 11 individuals were recaptured at points deviated to all directions from the releasing points. As the last, one individual was released with pop-up satellite archival tag at a costal point ($34^{\circ}37.2$'N, $124^{\circ}59.3$'E) off Hong Island on May 21, 2010. The tagged individual migrated to a north-east location ($35^{\circ}50.4$'N, $126^{\circ}03.6$'E) of Eocheong Island by Aug. 25, 2010. The data archived for the three months in the tag indicated that the migration path had depths of 48-80 m and temperature of $12.6-14.4^{\circ}C$. The results indicated that mottled sake populations had a localized habitat ranges at the north of Heuksan Island and the west of Hong Island while growing at the rate of $1.4cm\;mon^{-1}$.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.9
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• pp.2118-2135
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• 2012

With the rapid development of heterogeneous emerging wireless technologies and numerous types of mobile devices, the need to support robust mobile video streaming based on the seamless handover in Future Internet is growing. To support the seamless handover, several IP-based mobility management protocols such as Mobile IPv6 (MIPv6), fast handover for the MIPv6 (FMIPv6), Hierarchical MIPv6 (HMIPv6) and Proxy Mobile IPv6 (PMIPv6) were developed. However, MIPv6 depreciates the Quality-of-Service (QoS) and FMIPv6 is not robust for the video services in heterogeneous emerging wireless networks when the Mobile Node (MN) may move to another visited network in contrast with its anticipation. In Future Internet, the possibility of mobile video service failure is more increased because mobile users consisting of multiple wireless network interfaces (WNICs) can frequently change the access networks according to their mobility in heterogeneous wireless access networks such as 3Generation (3G), Wireless Fidelity (Wi-Fi), Worldwide Interoperability for Microwave Access (WiMax) and Bluetooth co-existed. And in this environment, seamless mobility is coupled according to user preferences, enabling mobile users to be "Always Best Connected" (ABC) so that Quality of Experience is optimised and maintained. Even though HMIPv6 and PMIPv6 are proposed for the location management, handover latency enhancement, they still have limit of local mobility region. In this paper, we propose a robust mobile video streaming in Heterogeneous Emerging Wireless Systems. In the proposed scheme, the MN selects the best-according to an appropriate metric-wireless technology for a robust video streaming service among all wireless technologies by reducing the handover latency and initiation time when handover may fail. Through performance evaluation, we show that our scheme provides more robust mechanism than other schemes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.335-339
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• 2006

The level of ${\beta}-glucan$ which is a major soluble dietary fiber found in the grain endosperm cell wall was highly variable among 25 barley genotypes grown at four locations including Suwon, Naju, Jinju, and Jeju. Statistically significant genotypic effects were observed for ${\beta}-glucan$ content at each or across growing sites (P<0.001). On average, 'Chalssalbori' showed the lowest percentage ${\beta}-glucan$ (4.04%) among genotypes in the grain, whereas 'Yonezawa Mochi' was highest in percentage ${\beta}-glucan$ (6.46%) compared to other genotypes. The significant difference between genotypes was approximately 1-2% across environments. The effects of location or interaction between locations and genotypes were not significant on the variation of ${\beta}-glucan$ contents. High ${\beta}-glucan$ content seemed to be greatly associated with such grain traits as waxiness and presence of husk except for 'Chalssalbori'. The waxy genotypes had a mean of 5.37% and values ranging from 5.28 to 5.47%, but normal genotypes had a mean of 4.78% and values ranging from 4.69 to 4.88% over environments. Hulless barley genotypes were also higher than hulled barley genotypes for the average ${\beta}-glucan$ content in both individual and over all environments. The difference between the hulled and hulless gene pools was on average of 0.37% with ranges from 0.19% to 0.56% at four environments. ${\beta}-glucan$ content measured from a mapping population of $F_5$-derived 107 lines derived from the cross between 'Yonezawa Mochi' and 'Neulssalbori' was not significantly associated with other agronomic traits except for 1,000-kernel weight at the '01 Suwon environment. Not too much information on the relationship of ${\beta}-glucan$ content to agronomic traits was available.

• Journal of Korea Technology Innovation Society
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• v.14 no.3
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• pp.647-663
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• 2011

Company's interest in the work place training and investment has been growing continuously because the talent of human resource is the competitiveness itself in the knowledge based society. However, corporate training programs mainly have focused on large companies and SMEs despite the economic business volume have been treated too lightly so far. This paper regards corporate training programs with one of the methods for SMEs innovation and proposes the smart learning in the smart device diffusion. Concretely, this paper analyzes the utilization intention, each attribute and level in smart learning characteristics using conjoint analysis. The result shows that SMEs have positive response for smart learning acceptance and SMEs consider significantly the usage fee type and location with the difference between regular employee and administrator. Specially, interactive communication and customized contents are preferred in the training type. Smart learning can be used as strategic means in supporting the value innovation and enhancing the absorptive capacity in SMEs innovation process.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1614-1623
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• 2010

Bacteria were isolated from roots of sugarcane varieties grown in the fields of Punjab. They were identified by using API20E/NE bacterial identification kits and from sequences of 16S rRNA and amplicons of the cpn60 gene. The majority of bacteria were found to belong to the genera of Enterobacter, Pseudomonas, and Klebsiella, but members of genera Azospirillum, Rhizobium, Rahnella, Delftia, Caulobacter, Pannonibacter, Xanthomonas, and Stenotrophomonas were also found. The community, however, was dominated by members of the Pseudomonadaceae and Enterobacteriaceae, as representatives of these genera were found in samples from every variety and location examined. All isolates were tested for the presence of five enzymes and seven factors known to be associated with plant growth promotion. Ten isolates showed lipase activity and eight were positive for protease activity. Cellulase, chitinase, and pectinase were not detected in any strain. Nine strains showed nitrogen fixing ability (acetylene reduction assay) and 26 were capable of solubilizing phosphate. In the presence of 100 mg/l tryptophan, all strains except one produced indole acetic acid in the growth medium. All isolates were positive for ACC deaminase activity. Six strains produced homoserine lactones and three produced HCN and hexamate type siderophores. One isolate was capable of inhibiting the growth of 24 pathogenic fungal strains of Colletotrichum, Fusarium, Pythium, and Rhizoctonia spp. In tests of their abilities to grow under a range of temperature, pH, and NaCl concentrations, all isolates grew well on plates with 3% NaCl and most of them grew well at 4 to $41^{\circ}C$ and at pH 11.