• Journal of Microbiology and Biotechnology
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• v.21 no.4
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• pp.374-378
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• 2011

To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using double-crossover, for use as a screening host. The W3110 dsdA::tet strain was not able to grow in a medium containing D-serine as a sole carbon source, whereas wild-type W3110 and the complement W3110 dsdA::tet strain containing a dsdA-expression plasmid were able to grow. After introducing metagenome libraries into the screening host, a strain containing a 40-kb DNA fragment obtained from the metagenomic souce derived from a compost was selected based on its capability to grow on the agar plate containing D-serine as a sole carbon source. For identification of the genetic resource responsible for the D-serine degrading capability, transposon-${\mu}$ was randomly inserted into the 40-kb metagenome. Two strains that had lost their D-serine degrading ability were negatively selected, and the two 6-kb contigs responsible for the D-serine degrading capability were sequenced and deposited (GenBank code: HQ829474.1 and HQ829475.1). Therefore, new alternative genetic resources for D-serine dehydratase was found from the metagenomic resource, and the corresponding ORFs are discussed.

• The Journal of the Korea Contents Association
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• v.15 no.9
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• pp.639-651
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• 2015

The purpose of this study is to examine the effects of knowledge base and metamemory in children's conceptual implicit memory with category-exemplar-generation task. Subjects were total 180 children of each 60 from Grade2, Grade6 and High school students. They were examined implicit memory with category-exemplar-generation task, knowledge base test, and metamemory test. The data were analyzed using ANCOVA, and Scheffe post hoc test. The result was following: First, as the child grow old, implicit memory primed increased. Implicit memory amount was significantly different between Grade2 and High school students, Grade6 and High school students. Second, as the child grow high knowledge base, implicit memory primed increased. There was a significantly different found between age and knowledge base. Third, as the child grow high metamemory, implicit memory primed increased. These results were interpreted as that the state of the age, knowledge base and metamemory should be an important factorin implicit memory. And current findings suggest that implicit memory can show development if a children's knowledge base and metamemory in developing with age.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.489-492
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• 2000

The eventual goal of this study is to elucidate roles of plant terpenoids (e.g., cymene, limonene and others) as natural substrates in the cometabolic biodegradation of PCBs and to develop an effective PCB bioremediation technology. The aim of this study was to examine how plant terpenoids, as natural substrates or inducers would affect the biodegradation of PCB congeners. Various PCB degraders that could grow on biphenyl and several terpenoids were tested for their PCB degradation capabilities. The PCB congener degradation activities were first monitored through resting cell assay technique that could detect degradation products of the substrate. The congener removal was also confirmed by concommitant GC analysis. The PCB degraders, Pseudononas sp. P166 and Caynebacterium sp. T104 were found to grow on both biphenyl and terpenoids ((S)-(-) limonene, p-cymene and ${\alpha}-terpinene$) whereas Arthrobacter B1B could not grow on the terpenoids as a sole carbon source. The strain B1B grown on biphenyl showed a good degradation activity for 4,4'-dichlorobiphenyl (DCBp) while strains P166 and T104 gave about 25% of B1B activity. Induction of degradation by cymene, limonene and terpine was hardly detected by the resting cell assay technique. This appeared to be due to relatively lower induction effect of these terpenoids compared with biphenyl. However, a subsequent GC analysis showed that the congener could be removed up to 30% by the resting cells of T104 grown on the terpenoids. This indicates that terpenoids, widely distributed in nature, could be utilized as both growth and/or inducer substrate for PCB biodegradation.

• Journal of Life Science
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• v.13 no.6
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• pp.933-942
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• 2003

CoenzymeQ is a quinone derivative with a long isoprenoid side chain. It transports electrons in the respiratory chain located in the inner mitochondrial membrane of eukaryotes and the plasma membrane of prokaryotes. It also functions as an antioxidant. Saccharomyces cerevisine coq mutants, that are deficient coenzyme Q biosynthesis fail to aerobically grow. They are not able to grow on non-fermentable carbon sources, such as glycerol, either The putative $coq^{-7}$ gene involved in coenzyme Q biosynthesis of Neurospora crassa was cloned and used for complementation of S. cerevisiae coq7 mutant. The predicted amino acid sequence of N. crassa COQ7 showed about 58% homology with Coq7p of S. cerevisiae. The growth rate of S. cerevisiae $coq^7$ mutant transformed with the N. crassa $coq^{-7}$ gene was restored to the wild-type level. The complemented 5. cerevisiae strain was able to grow with glycerol as a sole carbon source and showed less sensitivities to linolenic acid, a polyunsaturated fatty acid.

• Journal of Microbiology
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• v.43 no.4
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• pp.325-330
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• 2005

Rhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes 0- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage path-way. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a $98\%$ identity to the gene, encoding meth-ylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJY J1

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.5
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• pp.2678-2688
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• 2014

The purpose of this study is to examine the explanatory power of factors similar change to see what the value of the child's father give faith, grow, grow what kind of participation in the developmental characteristics of infants affected. 1,802 people infants born in 2010, the study is his father, I used the data for the next fiscal year 3 Panel Korea Policy Institute childcare children that target it. Analysis of the data, we have carried out frequency analysis using SPSS18.0, The value of the infant's father a child problem-solving skills, fine motor activity been found to affect the activity. Muscle activity for the father of the infant did not influence variables are. And the infant's communication, personal-social variables that affect the independence turned out to be a father involved in parenting.

• Journal of Korean Society of Forest Science
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• v.7 no.1
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• pp.40-44
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• 1968

1. The larvae of the pine gall-midge begin to grow slowly from early July but grow rapidly from September. 2. The number of larvae in the galls does not decrease during the feeding period. 3. Pine needles infested by pine gall-midge cease to grow as soon as the gall starts to form. 4. In Seoul district the effective felling season of the pine trees for the Control of the pine gall-midge seems to be around 20th September when the size of larvae reaches 1.4-1.5mm in length. 5. The stratification and direction of the Crown, and the length of new shoot had no effect on the degree of infestation by pine gall-midge. However the degree of infestation differed among the trees of different ages as well as at the different levels of the mountain.

• Animal Bioscience
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• v.34 no.1
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• pp.36-47
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• 2021

Objective: This study determined the optimal ratio of whole plant corn silage (WPCS) to corn stover (stems+leaves) silage (CSS) (WPCS:CSS) to reach the greatest profit of dairy farmers and evaluated its consequences with corn available for other purposes, enteric methane production and milk nitrogen efficiency (MNE) at varying milk production levels. Methods: An optimization model was developed. Chemical composition, rumen undegradable protein and metabolizable energy (ME) of WPCS and CSS from 4 cultivars were determined to provide data for the model. Results: At production levels of 0, 10, 20, and 30 kg milk/cow/d, the WPCS:CSS to maximize the profit of dairy farmers was 16:84, 22:78, 44:56, and 88:12, respectively, and the land area needed to grow corn plants was 4.5, 31.4, 33.4, and 30.3 ha, respectively. The amount of corn available (ton DM/ha/yr) for other purposes saved from this land area decreased with higher producing cows. However, compared with high producing cows (30 kg/d milk), more low producing cows (10 kg/d milk) and more land area to grow corn and soybeans was needed to produce the same total amount of milk. Extra land is available to grow corn for a higher milk production, leading to more corn available for other purposes. Increasing ME content of CSS decreased the land area needed, increased the profit of dairy farms and provided more corn available for other purposes. At the optimal WPCS:CSS, MNE and enteric methane production was greater, but methane production per kg milk was lower, for high producing cows. Conclusion: The WPCS:CSS to maximize the profit for dairy farms increases with decreased milk production levels. At a fixed total amount of milk being produced, high producing cows increase corn available for other purposes. At the optimal WPCS:CSS, methane emission intensity is smaller and MNE is greater for high producing cows.

• Journal of People, Plants, and Environment
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• v.24 no.5
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• pp.451-460
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• 2021

Background and objective: The prolonged COVID-19 pandemic has had significant impacts on mental health, which has emerged as a major public health issue around the world. This study aimed to analyze trends and network structure of 'grow-your-own (GYO)' through Instagram, one of the most influential social media platforms, to encourage and sustain home gardening activities for promotion of emotional support and physical health. Methods: A total of 6,388 posts including keyword hashtags '#gyo' and '#growyourown' on Instagram from June 13, 2020 to April 13, 2021 were collected. Word embedding was performed using Word2Vec library, and 7 clusters were identified with K-means clustering: GYO, garden and gardening, allotment, kitchen garden, sustainability, urban gardening, etc. Moreover, we conducted social network analysis to determine the centrality of related words and visualized the results using Gephi 0.9.2. Results: The analysis showed that various combinations of words, such as #growourrownfood, #growourrownveggies, and #growwhatyoueat revealed preference and interest of users in GYO, and appeared to encourage their activities on Instagram. In particular, #gardeningtips, #greenfingers, #goodlife, #gardeninglife, #gardensofinstagram were found to express positive emotions and pride as a gardener by sharing their daily gardening lives. Users were participating in urban gardening through #allotment, #raisedbeds, #kitchengarden and we could identify trends toward self-sufficiency and sustainable living. Conclusion: Based on these findings, it is expected that the trend data of GYO, which is a form of urban gardening, can be used as the basic data to establish urban gardening plans considering each characteristic, such as the emotions and identity of participants as well as their dispositions.

• ALGAE
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• v.37 no.2
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.