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Characterization of Yellow Mutants Isolated from the Red Yeast Phaffia rhodozyma (Xanthophyllomyces dendrorhous)

  • An, Gil-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.6 no.2
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    • pp.110-115
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    • 1996
  • Yellow mutants of the astaxanthin producing red yeast Phaffia rhodozyma were obtained by nitrosoguanidine mutagenesis. The carotenoid composition of the yelow mutants, Yan-1 and Ny-1, was mainly $\beta$ -carotene (> 95$%$) and torulene (< 5$$). Therefore, the yellow mutants are carotene oxygenation deficient mutants (CODMs). CODMs produced decreased quantities of carotenoids compared to their red parents and this indicated that carotene may regulate its synthesis. CODMs, Yan-1 and Ny-4, on plates containing 50 $\mu$ M antimycin, showed highly pigmented vertical papillae. Antimycin-induced mutants purified from the papillae showed increases in carotenoid content (up to 1 mg $\beta$-carotene/g yeast). CODMs, Yan-1 and Ay-1, were more sensitive to antimycin than red strains, Ant-1 and 67-385. This was probably due to lower antioxidant activity of $\beta$-carotene than that of astaxanthin. Light increased torulene and light+antimycin further increased the torulene. Yan-1 and Ny-4 could grow with succinate, though their red parents, Ant-1 and Anf-1p, could not. However, antimycin induced mutation of Yan-1 or Ny-4 destroyed the ability to grow with succinate.

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A Study on Proposal and Necessity to Adopt the LVDC Standardization Policy (저압직류전기설비 표준화 정책 도입의 필요성 및 제안에 관한 연구)

  • Oh, Du-Seok;Kang, Seung-Jin
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.29 no.1
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    • pp.121-127
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    • 2015
  • Recently, LVDC(Low Voltage Direct Current) has been came into the newly spotlight with HVDC(High Voltage Direct Current) and as increasing digital load, DC power supply and high quality of demand is also getting extended. In accordance with the report of EPRI(Electric Power Research Institute), they expect digital load will grow to 50% of whole load in 2020. Subsequently, the use of DC equipment will grow dramatically. Therefore, the careful adoption of LVDC standardization policy is urgently required for the safety of people, prevention of confusion and occupancy of the market.

Microstructural improvement in polycrystalline Si films by crystallizing with vapor transport of Al/Ni chlorides

  • Eom, Ji-Hye;Lee, Kye-Ung;Jun, Young-Kwon;Ahn, Byung-Tae
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.315-318
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    • 2004
  • We developed a vapor induced crystallization (VIC) process for the first time to obtain high quality polycrystalline Si films by sublimating the mixture of $AlCl_3$ and $NiCl_2$. The VIC process enhanced the crystallization of amorphous silicon thin films. The LPCVD amorphous silicon thin films were completely crystallized after 5 hours at 480 $^{\circ}C$. It is known that needle-like grains with very small width grow in the Ni-metal induced lateral crystallization. In our new method, the width of grains is larger because the grain can also grow perpendicular to the needle growth direction. Also the interface between the merging grain boundaries was coherent. As the results, a polycrystalline film with superior microstructure has been obtained.

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Physiological Studies on the Function of Biological Membrane: Structural Changes of the Vitelline Envelopes during Oogenesis of a Polychaete, Nectoneanthes oxypoda (생체막의 기능에 대한 생리학적 연구: 갯지렁이 Nectoneanthes oxypoda의 난자형성단계에 따른 난황막의 구조적 변화)

  • Lee, Yang-Rim
    • Applied Microscopy
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    • v.20 no.2
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    • pp.117-126
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    • 1990
  • Structural changes of the vitelline envelopes during oogenesis of a polychaete, Nectoneanthes oxypoda, were examined with a scanning electron micrscope. Oocytes grow in the same coelomic fluid to the final stage, but the surface appears to change in the structure during oogenesis. Projections, which were identified to be microvilli, change in shape, number and size. Short microvilli, which cover the surface of oocyte of $33{\mu}m$ diameter densely, grow in length, reaching a maximum at the stage of $73{\mu}m$. The number of microvilli increases with the stages of oogenesis, reaching a plateau at the stage of $82{\mu}m$. The observations suggest that control of material transport including yolk precursor proteins may be correlated with the structural changes in the microvilli.

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A Study on the Change of the dwelling Space according to the Growth of Children -Focused on the Correspondence of the Family Life Cycle to dwelling Space- (자녀의 성장과정에 따른 주공간 사용 변화에 관한 연구-가족형태 변화와 주공간의 대응관계를 중심으로-)

  • 박현아;박용환
    • Journal of the Korean housing association
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    • v.12 no.4
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    • pp.43-52
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    • 2001
  • The purpose of this study is to fined out the characteristics of using & change of the residential space according to the children age and family life cycle. The results of this study are as follows: The first, as children grow up, children's activity space changed from living room to the private room, and housing condition was related to the frequency of each activity. The second, as children grow up, family's sleeping pattern changed, and this change effected the number of private rooms for sleeping and the use of the extra room. The third, changes of the using of dwelling space come out several pattern, increasing bedroom number through reconstruction, exchange of An-band and children room, parents' sleeping space move to living room, and change the function of rooms. The change pattern of the correspondence to the housing needs differed from each family life cycle stage.

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Cloning and Nucleotide Sequence of the recA Gene from Shigella sonnei KNIH104S Isolated in Korea

  • Park, Yong-Chjun;Shin, Hee-Jung;Kim, Young-Chang
    • BMB Reports
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    • v.32 no.5
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    • pp.436-439
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    • 1999
  • Shigella sonnei is an important cause of human enteric infections. S. sonnei KNIH104S was previously reported to be isolated from Korean shigellosis patients. We cloned a 2.8-kb KpnI fragment containing the recA gene encoding a recombinase from the chromosomal DNA of S. sonnei KNIH104S. This recombinant plasmid was named pRAK28. E. coli HB101, a recA mutant, cannot grow on Luria-Bertani medium in the presence of the alkylating agent methylmethane sulfonate, however, E. coli HB101 harboring pRAK28 was found to grow on this medium. As far as we know, we are the first to sequence the recA gene from S. sonnei. This gene is composed of 1062 base pairs with an ATG initiation codon and a TAA termination codon. Nucleotide sequence comparison of the S. sonnei recA gene exhibited 99.7% and 99.5% identity with those of S. flexneri and E. coli, respectively.

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Bottle Cultivation of Pleurotus ostreatus, Agrocybe aegerita and Ganoderma lucidum using Rice hull media

  • Lee, he-duck;Kim, hong-kyu;Kim, yong-gyun;Lee, ga-soon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2001.04a
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    • pp.44-46
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    • 2001
  • Rice hull was used as a additive in order to find the effect for incresing of mushroom growth and yield in Chungnam Provincial techinical institution. 1 Treatment of 80% rice hull in small Neutaribeosut mycelial grow duration is shorter about 11 days and yield increased about 7% than conventional culture. 2. In case of Chongpung Neutaribeosut bottle culture, mycelial growth duration is shorter about two to three days in additive of 30 to 80% rice hull compared to conventional but yield similar to conventional. 3. Treatment of 30% rice hull in Agrocybe aegerita bottle culture, mycelial growth and yield increased 6days and 6% than convrntional, respectively 4 Treatment additived of 30% to 40% rice hull in Ganoderma lucidum bottle culture, similar to 454ays demand in mycelial grow duration and 38g yield/bottle in conventioal culture methods.

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Studies on the Preparation of Phosphorescent Materials (당광체에 관한 연구)

  • 한관섭
    • YAKHAK HOEJI
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    • v.7 no.1
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    • pp.26-29
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    • 1963
  • Three systems such as CaS, BaS and ZnS are used as ground materials and transition elements such as Bi, Cu, Ag and Ni are used as activators to preared phosphorescent materials of strong after-grow intensity. These three systems in which we have 31 kinds of different composition samples are heated at given temperatures in the electrical furnace and are cooled in the air in order to crystallize them. In the case of BaS system, the mixture of CaO and purified S is better than the other kinds of calcium compound as the ground materials to crystallize phosphors. In BaS system, the mixture of BaCO$_{3}$ and purified S, in ZnS system ZnS respectively is the most proper ground material to prepare such a strong after-grow intensified phosphors. In a given range, the higher in temperature and the longer time of heating, the better phosphors we obtain.

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The Growth of LiNbO3 Crystals by Czochralski Technique (Czochralski법에 의한 $LiNbO_3$ 단결정 성장)

  • 이상학;윤의박
    • Journal of the Korean Ceramic Society
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    • v.29 no.3
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    • pp.189-194
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    • 1992
  • In order to grow a 127.86$^{\circ}$rotated LiNbO3 single crystal with good characteristics of surface acoustic wave (SAW) up to 80 mm in diameter, the temperature gradient of furnace, the growth rate and the rotation rate of crystal were changed. We could grow a crystal which had few macro defects at the conditions of temperature gradient as 30~6$0^{\circ}C$/cm, growth rate as 5 mm/hr and rotation rate as 8 rpm. The experimental ranges of the growth conditions are as follows. Temperature gradient was varied from 20 to 20$0^{\circ}C$/cm, growth rate as 5~7 mm/hr and crystal rotation rate as 6~12rpm.

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Stress Intensity Factors for Elliptical Arc Through Cracks in Mechanical Joints by Virtual Crack Closure Technique

  • Heo, Sung-Pil;Yang, Won-Ho;Kim, Cheol
    • Journal of Mechanical Science and Technology
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    • v.16 no.2
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    • pp.182-191
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    • 2002
  • The reliable stress intensity factor analysis is required for fracture mechanics design or safety evaluation of mechanical joints at which cracks often initiate and grow. It has been reported that cracks in mechanical joints usually nucleate as corner cracks at the faying surface of joints and grow as elliptical arc through cracks. In this paper, three dimensional finite element analyses are performed for elliptical arc through cracks in mechanical joints. Thereafter stress intensity factors along elliptical crack front including two surface points are determined by the virtual crack closure technique. Virtual crack closure technique is a method to calculate stress intensity factor using the finite element analysis and can be applied to non-orthogonal mesh. As a result, the effects of clearance on the stress intensity factor are investigated and crack shape are then predicted.