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해양생태계 모니터링을 위한 식물플랑크톤 자료의 정도 관리 동향 (Trends in QA/QC of Phytoplankton Data for Marine Ecosystem Monitoring)

  • 이원호;박종우;성경아;박종규;유영두;김형섭
    • 한국해양학회지:바다
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    • 제26권3호
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    • pp.220-237
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    • 2021
  • 1880년대에 들어서서 해양 식물플랑크톤의 기능적 중요성이 처음 주창된 이래, 전통적인 형태 관찰법 및 진보된 형태 자동분석 기술을 기반으로 하여 다량의 식물플랑크톤 종별 정량자료가 생산되었다. 최근에는 해수시료 중의 색소를 직접 분석하거나 원격탐사 자료를 해석하여 색소특성에 따른 분류군별 정량자료를 생산함으로써, 자료생산 방법이 점점 다양해지고 자료 확보 대상 정점에 대한 시공간적 접근성도 크게 개선되고 있다. 장기적인 해양생태계 모니터링에서 식물플랑크톤의 종별 정량자료가 생산된 경우도 적지 않아, 각각의 해역에서 중장기적인 해양 식물플랑크톤의 구조와 기능의 변동에 대한 중요한 증거로 활용될 수 있다. 그러나 모니터링 기간 전체에 걸친 연대별 자료 생산자 간의 차이로 인해 이러한 자료의 활용성이 제한될 수 있는데, 시료 처리 및 분석법, 종의 확인 및 분류, 분석이 완료된 시료의 관리 등의 다양한 측면에서 연대별 생산자 간의 편차가 적지 않다. 해양 식물플랑크톤의 종별 정량자료 값을 정확하게 구하기 위한 심도있는 연구는 1880년대 후반 Victor Hensen이 시작한 것으로 평가된다. 정확도를 포함한 해양 식물플랑크톤 자료의 정도 관리에 관한 국제적인 논의는 ICSU의 SCOR Working Group 33을 중심으로 1969년에 시작되었다. 첫 결실로 UNESCO 해양과학기술보고서 제18편이 1974년 출판되었는데, 이는 UNESCO의 해양학 방법론의 전문연구서적 제6편인 Phytoplankton Manual 출판의 실마리였다. 1990년대 말에는 ISO 기준에 따른 해양 식물플랑크톤 종별 정량자료의 정도관리를 달성하여, 국제적인 자료의 상호비교 및 교정을 가능하게 하려는 수행기구인 IPI (International Phytoplankton Intercomparison)의 전신인 BEQUALM 사업이 유럽에서 본격 출범하였다. IPI는 지난 20여 년간의 경험과 실적을 바탕으로 정도관리 기준을 모든 나라에서 적용할 수 있도록 국제협력을 강화해 나가고 있다. 우리나라의 해양화학 분야 측정자료의 정도관리 체계와 내용이 잘 정립된 데 비하여, 해양생물의 종별 정량자료에 대한 본격적인 정도관리 체계는 아직 법제화 단계에 이르지 못하고 있다. 우선, 해양생태계의 기초 생산자인 식물플랑크톤의 종별 정량자료에 대한 정도관리 체계를 확립하고, 다양한 기능생물군으로 이를 확장해 나갈 필요가 있다.

Effect of Phase Feeding on the Growth Performance, Nutrient Utilization and Carcass Characteristics in Finishing Pigs

  • Lee, J.H.;Kim, J.D.;Kim, J.H.;Jin, J.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권8호
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    • pp.1137-1146
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    • 2000
  • This study was carried out to establish an optimum number of phase feeding regimen which enable to reduce nutrients excretion without affecting growth performance and to investigate the effects of different feeding regimens on growth performance, nutrients excretion and carcass characteristics in finishing pigs. A total of 120 finishing pigs (an average initial body weight of 54.3 kg) were assigned to the feeding trial and 12 pigs were assigned to the metabolic trial. Treatments included one phase (54 to 104 kg), two phase (54 to 80 and 80 to 104 kg), three phase (54 to 70, 70 to 90 and 90 to 104 kg) and four phase (54 to 65, 65 to 80, 80 to 95, 95 to 104 kg) feeding regimens. Experimental diets were formulated to contain 16% crude protein for one phase feeding regimen, 16% and 12% crude protein for two phase feeding regimen, 16%, 14% and 12% crude protein for three phase feeding regimen, and 16%, 14.7%, 13.4% and 12% crude protein for four phase feeding regimen, respectively. Although there were no significant differences in any criteria measured during the entire experimental period, pigs reared in three phase feeding regimen grew slightly faster than those reared in other feeding regimens and showed a tendency to increase ADFI during the whole experimental period. The metabolic trial indicated that there were no significant differences in DM (dry matter), CP (crude protein) and P (phosphorus) digestibilities. However, fecal nutrient excretion except P was significantly influenced by feeding regimens. DM excretion of one phase feeding group was significantly higher than that of three phase feeding group and daily fecal N (nitrogen) excretion of one phase feeding group was higher than that of other phase feeding groups (p<0.05). Three and four phase feeding regimens resulted in 12% lower fecal N and DM excretion than one phase feeding regimen. Blood urea concentrations were lower for pigs reared in two, three and four phase feeding regimens than for those reared in one phase feeding regimen (p<0.05). Three phase feeding regimen for the finishing period showed better carcass grade than one phase feeding regimen, though the difference was not significant. The tenth rib fat thickness of pigs fed on four phase feeding regimen was reduced most and there was a trend that backfat thickness decreased as the number of phases increased. Feed cost per kg weight gain was significantly low in four phase feeding group than one phase feeding group (p<0.05). In summary, it seemed that producers generally oversupply the expensive nutrients for the finishing pigs. High nutrient diets do not always guarantee high growth rate of pigs and cause more unwanted nutrient excretion. It rather seems that meeting nutrient requirements for the each growth phase is more important for the reduction of pollutants and economical pork production.

The Causes of Guilt in Ready-meal Users: A Focus on Cooking Instructions and Consumers' Health Locus of Control

  • Shin, Hyunsook;Lee, Dongmin;Lim, Jeeyoung;Moon, Junghoon
    • Asia Marketing Journal
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    • 제21권4호
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    • pp.25-43
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    • 2020
  • Although ready meals have recently increased their market share in the Korean food industry, a literature review found that the use of ready meals triggers feelings of guilt in homemakers. Such guilt arises as a result of several factors apparently related to consumers' health. Consequently, levels of guilt might be expected to vary depending on consumers' perceived health locus. The present study aims to examine (a) how health locus affects guilty feelings about ready-meal consumption, (b) how the effect varies in relation to the consumption of different types of ready meal, and (c) the relationship between consumers' guilty feelings and willingness to buy ready meals. Three dimensions of health locus of control (HLC) -internal HLC (IHLC), powerful-others HLC (PHLC), and chance HLC (CHLC)- were presumed to influence consumers' feelings of guilt in association with ready meals. Data were collected via an online survey, and participants were randomly assigned to either of two groups: one group was instructed to heat meals in a microwave (ready-to-heat [RTH] group, n=104) and the other cooked using a pan with additional ingredients (ready-to-cook [RTC] group, n=101). The study found that guilty feelings about consuming RTH meals increased in line with increased external HLCs, namely, PHLC and CHLC. For the RTC group, guilt increased in line with increased PHLC. IHLC had no significant effect on guilty feelings in either group. Willingness to buy ready meals decreased for both groups as consumers' feelings of guilt increased. Even RTC meals, which require more time and energy in food preparation, did not reduce guilty feelings among consumers with higher PHLC. RTC meals are preferable for consumers with higher CHLC, since their sense of greater involvement in the cooking process alleviates their feelings of guilt. Cooking with already prepared and uncooked ingredients brought fun and joy, both for the participants and their significant others. This interpretation may be developed into a strategic plan by ready-meal producers to strengthen their marketing strategy.

과학자의 탐구 과정을 재구성한 광합성 실험 모듈의 개발과 적용 (Development and Application of the Photosynthesis Experimental Module Based on Scientist's Inquiry Processes)

  • 김호기;김연주;김성하
    • 과학교육연구지
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    • 제35권2호
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    • pp.204-220
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    • 2011
  • 이 연구는 초기 광합성 연구자의 탐구 과정을 재구성하여 중학생들의 과학탐구능력과 광합성 관련 개념의 향상을 위한 실험 모듈을 개발한 후, 이의 적용 효과를 알아보고 광합성과 관련된 학생들의 선행 개념 유형과 개념 변화의 패턴을 분석하고자 하였다. 실험재료로는 Rapid-cycling Brassica rapa 라는 속성식물을 사용하였다. 개발된 모듈을 적용한 실험반의 수업은 교과서에 제시된 실험을 적용한 통제반의 수업에 비해 학생들의 과학탐구능력 향상에 효과적이었으며, 과학탐구능력의 하위 요소 중에서 측정, 예상, 가설 설정 능력의 향상에 효과적이었다. 실험반의 사전-사후 검사 결과를 비교해 본 결과, 관찰, 예상, 일반화 능력의 향상에 더욱 효과가 있는 것으로 나타났다. 개발된 모듈은 광합성 관련 개념 성취 및 생태계내에서의 식물의 역할, 식물의 먹이, 식물체 내에서 물과 양분의 이동과 관련된 개념 발달에 효과가 있었다. 학생들은 선행 개념으로 이산화탄소나 산소와 같이 눈에 보이지 않는 물질의 존재나 역할 또는 에너지의 축적과 같은 추상적 개념에 대한 이해가 부족한 것으로 나타났으나, 이 연구에서 개발된 모듈을 적용한 결과 유기물과 산소 생산자인 식물의 역할에 대한 포괄적인 개념 발달에 효과가 있었으며, 과학탐구능력과 광합성 관련 개념의 향상에 효과가 있었다.

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Estimation of Protein Deposition Rate of Growing-Finishing Pigs Reared in Commercial Conditions in Korea

  • Kim, J.H.;Sohn, K.S.;Hynn, Y.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권8호
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    • pp.1147-1153
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    • 2000
  • A total of 9,540 pigs were evaluated for their growth performance to provide information on the development of different feeding strategies to support maximum rate of protein deposition (PD). Large variations in growth performance and protein deposition rate were found in the population used in this study (ADG from 701 to 974 g/day; ADFI from 1,726 to 2,498 g/day; Feed/gain from 2.10 to 2.90; Backfat thickness from 12.4 to 20.5 mm and PD rate from 103 to 153 g/day). It was found that ADG was positively correlated to PD ($R^2=0.9362$, p<0.0001) while FCR was negatively correlated to PD ($R^2=0.4031$, p<0.0001). Backfat thickness was negatively correlated to PD ($R^2=0.7024$, p<0.0001) and to ADG ($R^2=0.5096$, p<0.0001). The estimated lysine requirement based on PD rate also showed large variation (12.37 to 18.38 g/day true ileal digestible lysine on average between 25 and 100 kg), thus strongly indicated the need of separate feeding strategies for each group of pigs. When pigs were divided into three categories according to estimated whole body PD rate, the group of pigs with the highest PD rate grew faster by 6.3 and 13.9% than pigs with intermediate and low PD rate, respectively. Feed utilization was also more efficient in pigs with a high PD rate. It appeared that pigs with high PD rate maintained higher PD rate especially in the later stage of their life. Pigs with high PD rate require an extra amount of 1.2 and 2.4 g/true digestible lysine per day and 0.4 and 0.8% more lysine in the diet than pigs with intermediate and low PD rate during the growing-finishing period respectively. Results of this study suggest that there is a need for separate feeding strategies for individual group of pigs with different PD rate. It should be noted that average value for each group presented in this report is not the adequate amount for an animals potential for maximum PD rate. With recent development in growth modeling and access to computer technologies to facilitate computation, pork producers can easily estimate pigs protein deposition rate and thus can make their own feeding strategies.

The effect of nanoemulsified methionine and cysteine on the in vitro expression of casein in bovine mammary epithelial cells

  • Kim, Tae-Il;Kim, Tae-Gyun;Lim, Dong-Hyun;Kim, Sang-Bum;Park, Seong-Min;Lim, Hyun-Joo;Kim, Hyun-Jong;Ki, Kwang-Seok;Kwon, Eung-Gi;Kim, Young-Jun;Mayakrishnan, Vijayakumar
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권2호
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    • pp.257-264
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    • 2019
  • Objective: Dairy cattle nutrient requirement systems acknowledge amino acid (AAs) requirements in aggregate as metabolizable protein (MP) and assume fixed efficiencies of MP used for milk protein. Regulation of mammary protein synthesis may be associated with AA input and milk protein output. The aim of this study was to evaluate the effect of nanoemulsified methionine and cysteine on the in-vitro expression of milk protein (casein) in bovine mammary epithelial cells (MAC-T cells). Methods: Methionine and cysteine were nonionized using Lipoid S 75 by high-speed homogenizer. The nanoemulsified AA particle size and polydispersity index were determined by dynamic light scattering correlation spectroscopy using a high-performance particle sizer instrument. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to determine the cytotoxicity effect of AAs with and without nanoionization at various concentrations (100 to $500{\mu}g/mL$) in mammary epithelial cells. MAC-T cells were subjected to 100% of free AA and nanoemulsified AA concentration in Dulbecco's modified Eagle medium/nutrient mixture F-12 (DMEM/F12) for the analysis of milk protein (casein) expression by the quantitative reverse transcription polymerase chain reaction method. Results: The AA-treated cells showed that cell viability tended to decrease (80%) in proportion to the concentration before nanogenesis, but cell viability increased as much as 90% after nanogenesis. The analysis of the expression of genetic markers related to milk protein indicated that; ${\alpha}_{s2}$-casein increased 2-fold, ${\kappa}$-casein increased 5-fold, and the amount of unchanged ${\beta}$-casein expression was nearly doubled in the nanoemulsified methionine-treated group when compared with the free-nanoemulsified methionine-supplemented group. On the contrary, the non-emulsified cysteine-administered group showed higher expression of genetic markers related to milk protein ${\alpha}_{s2}$-casein, ${\kappa}$-casein, and ${\beta}$-casein, but all the genetic markers related to milk protein decreased significantly after nanoemulsification. Conclusion: Detailed knowledge of factors, such nanogenesis of methionine, associated with increasing cysteine and decreasing production of genetic markers related to milk protein (casein) will help guide future recommendations to producers for maximizing milk yield with a high level of milk protein casein.

Carcass characteristics and meat quality of purebred Pakchong 5 and crossbred pigs sired by Pakchong 5 or Duroc boar

  • Lertpatarakomol, Rachakris;Chaosap, Chanporn;Chaweewan, Kamon;Sitthigripong, Ronachai;Limsupavanich, Rutcharin
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권4호
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    • pp.585-591
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    • 2019
  • Objective: This study investigated carcass characteristics and meat quality of purebred Pakchong 5, crossbred pigs sired by Pakchong 5, and crossbred pigs sired by Duroc. Methods: Forty-eight pigs (average body weight of 22.25 kg) were composed of three groups as purebred Pakchong 5 (PP), Large $White{\times}Landrace$ pigs sired by Pakchong 5 (LWLRP), and Large $White{\times}Landrace$ pigs sired by Duroc (LWLRD). Each group consisted of eight gilts and eight barrows. At 109-day-raising period, pigs were slaughtered, and carcass characteristics were evaluated. Longissimus thoracis (LT) muscles from left side of carcasses were evaluated for meat quality and chemical composition. Data were analyzed using general linear model procedure, where group, sex, and their interaction were included in the model. Results: The PP had greater carcass, total lean, and ham percentages than crossbred pigs (p<0.05). LWLRP had thicker backfat and more carcass fat percentage than LWLRD (p<0.05). There were no differences (p>0.05) on cutting percentages from tender loin, loin, boston butt, and picnic shoulder among groups. The PP and LWLRP had larger loin eye area (LEA) than LWLRD (p<0.05). Gilts had more loin percentage and lower $L^*$ value than barrows (p<0.05). No meat color parameters ($L^*$, $a^*$, and $b^*$) were affected by groups (p>0.05). PP and LWLRP had larger muscle fiber diameters than LWLRD (p<0.05). However, water holding capacity, Warner-Bratzler shear force values, and chemical composition of LT were not affected by group or sex (p>0.05). Conclusion: Pakchong 5 purebred has good carcass and lean percentages. Compared to Duroc crossbred pigs, Pakchong 5 crossbreds have similar carcass and lean percentages, larger LEA, and slightly more carcass fat, with comparable meat quality and chemical composition. Pakchong 5 boars are more affordable for very small- to medium-scale pig producers.

Problems of Using Cyberdramaturgy in Modern Foreign Cinematography

  • Portnova, Tatiana V.
    • International Journal of Computer Science & Network Security
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    • 제22권10호
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    • pp.25-30
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    • 2022
  • The article is devoted to the actual problem of the use of digital technologies in modern cinema in developed countries. The purpose of the study is to identify the essence of the term "cyberdramaturgy" and the problems of its use in modern film production. The research methodology is based on a systematic approach and includes the methods of the general scientific group (analysis, synthesis, deduction, induction), as well as a number of special methods: the method of content analysis of scientific literature on the research topic; sociological survey method; as well as the method of statistical analysis. The results of the survey were analyzed using the Neural Designer program (a tool for advanced statistical analytics) and translated into a graphical diagram format for clarity of perception. Answers in 75 questionnaires were evaluated by the average score for six analysis criteria, which made it possible to bring all the calculations to a 10-point scale. As a result of the study, the author of the article concluded the following: directors believe that the use of cyber analogues of actors and backgrounds leads to the blurring of genres, the hybridization of cinema and animation; directors are also concerned about the problem of replacing the director himself with a special program. The writers are completely concerned with the problem of machine scripting with almost infinite variability beyond the human imagination. Directors-producers believe that the cyberdramaturgy development will lead to completely new standards of cinematic quality, sharply different from the traditional assessment of acting and scene setting, to the appreciation of 3D animation as the highest category in the art. Such innovations actually devalue all international cinematography awards, as cyberdrama reduces the value of cyberactors to zero. It is impossible to bail out an "Oscar" or a "Golden Globe" award for a digital double or a separate cyber model that is used in the film instead of the actors.

Mannose-resistant Hemagglutination(MRHA) 및 Colonization Factor Antigen I(CFA I)을 표현하는 대장균의 장관내 존재와 설사증 발현과의 관계 (Gastrointestinal Carriage of Escherichia coli with Hemagglutination Activity and Colonization Factor Antigen I and its Relation to Diarrhea)

  • 노승현;김경희;조양자;서인수
    • 대한미생물학회지
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    • 제22권2호
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    • pp.147-153
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    • 1987
  • Colonization factor antigen I(CFA I) has been shown to be one of several virulence factors that promote attachment of enterotoxigenic E. coli(ETEC) to small intestinal epithelial cells of humans. The ability of ETEC to produce mannose-resistant hemagglutination(MRHA) of human blood group A has been used to detect CFA I. To determine gastrointestinal carriage in Korean children of E. coli with MRHA and CFA I, 116 strains of E. coli from diarrheal children admitted to Hanyang University Hospital were examined for MRHA of human erythrocytes and the presence of CFA I. Of 45 ETEC strains, 18(40%) gave a positive MRHA($MRHA^+$) and eight(18%) were positive for CFA I(CFA $I^+$). ETEC with CFA I were all heat-stable enterotoxin(ST) producers and two of these strains were of serogroups $O_{25}$. Of 17 classic enteropathogenic E. coli(EPEC), 7(41%) were $MRHA^+$ but all were negative for CFA I(CFA $I^-$). Of 30 enteroadherent E. coli(EAEC) strains, 11(37%) were $MRHA^+$ and one was CFA $I^+$. Of 24 nonpathogenic E. coli, 4(17%) were $MRHA^+$ but all were CFA $I^-$. It was shown that MRHA was common in all strains of E. coli, CFA I was limited only to ST producing ETEC and EAEC; although MRHA is a useful screening procedure, serologic tests seem to be necessary to comfirm CFA I production. CFA I was associated with a lower proportion of ETEC isolates in Korea than has been reported for other locations.

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한국육계산업의 수직통합(계열화) 전개상황 (Evolution of Vertical Integration in the Korea Broiler Industry)

  • 박영인
    • 한국가금학회지
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    • 제18권1호
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    • pp.57-66
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    • 1991
  • The broiler industry in Korea has been gradually changing its structure of independent operation into integrated and coordinated production and marketing system. It was in 1960s when the broiler industry first tried the integrated effort, but never succeeded as it was expected since due mainly to growers' attitudes favorable for traditional operation and agribusiness' passive response on structure change. Until mid 1980s, however, a bit of partially integrated system had been evolved by several factor suppliers. Recent years have furthered its progress toward completely integrated pattern, the ultimate goal of broiler integration. It is true that the external factors including the GATT Negotiations through the Uruguay Round stimulated the people involved in the industry to find the way of making it competitive to survive in the open, liberalized world economic system rapidly emerging through the process of globalization. Once the industry realized the necessity of reducing the cost of production and improving the quality of it, the conclusion tended to come to the point of an entire structural alteration by means of integration rather than an improvement of a certain segment such as marketing. It is very obvious. therefore. that the movement toward the complete integration will speed up in a coming few years under the leadership of not only entrepreneurs but also producers group. According to a report. there were three almost complete integrators and thirteen partial integrators as of December, 1990, all of which eagerly directed toward the completely integrated system in due course . Among others as an advantage of integrated structure. the cost saving must be pointed out as the greatest one In a survey, it was estimated that the cost of dressed and ready-to-eat chicken could be lowered by 31.8 percent and 41.5 percent, respectively, over that to be under the independent, non-integrated system. As long as everybody concerned about the future of the industry in the open market mechanism realizes the importance of the structure change through an integration, the broiler sector of Korean agriculture shall be moving toward the direction of vertically and completely integrated structure in order to make it competitive, even to export, hopefully before 1995 or by the end of this century in any case.

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