• Title/Summary/Keyword: group detection

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Simultaneous Detection and Identification of Bacillus cereus Group Bacteria Using Multiplex PCR

  • Park, Si-Hong;Kim, Hyun-Joong;Kim, Jae-Hwan;Kim, Tae-Woon;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.17 no.7
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    • pp.1177-1182
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    • 2007
  • Bacillus cereus group bacteria share a significant degree of genetic similarity. Thus, to differentiate and identify the Bacillus cereus group efficiently, a multiplex PCR method using the gyrB and groEL genes as diagnostic markers is suggested for simultaneous detection. The assay yielded a 400 bp amplicon for the groEL gene from all the B. cereus group bacteria, and a 253 bp amplicon from B. anthracis, 475 bp amplicon from B. cereus, 299 bp amplicon from B. thuringiensis, and 604 bp amplicon from B. mycoides for the gyrB gene. No nonspecific amplicons were observed with the DNA from 29 other pathogenic bacteria. The specificity and sensitivity of the B. cereus group identification using this multiplex PCR assay were evaluated with different kinds of food samples. In conclusion, the proposed multiplex PCR is a reliable, simple, rapid, and efficient method for the simultaneous identification of B. cereus group bacteria from food samples in a single tube.

A Study on an Integer Frequency Offset Estimation and Compensation for DOCSIS 3.1 Downstream

  • Bae, JaeHwui;Song, JinHyuk;Ra, Sang-Jung;Choi, Dong-Joon;Jung, Joon-Young;Hur, Namho
    • IEIE Transactions on Smart Processing and Computing
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    • v.6 no.1
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    • pp.39-46
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    • 2017
  • In this paper, we propose an integer frequency offset estimation and compensation method based on PLC preamble correlation in DOCSIS 3.1 Downstream system. The proposed method determines the PLC preamble subcarrier location recovered from PLC data and the one obtained from PLC preamble correlation. We showed the performance of PLC preamble detection in the received signal through the maximum value detection of PLC preamble correlation. Thus we can estimate and compensate for the integer frequency offset by computing the difference of PLC subcarrier locations.

Exfoliation of Endometrial Cells on Cervicovaginal Smears (자궁경부 질 세포검사에서 관찰되는 자궁내막세포의 의의)

  • Kang, Mi-Seon;Yoon, Hye-Kyoung
    • The Korean Journal of Cytopathology
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    • v.13 no.1
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    • pp.1-7
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    • 2002
  • The significance of endometrial cells on cervicovaginal smears is underestimated. The aim of this study is to evaluate the detection rate of endometrial cells on cervicovaginal smears. The materials consisted of two groups. Group I was 701 cervicovaginal smears from patients with no gynecological problems. Group II was 208 cervicovaginal smears from patients with abnormal uterine bleeding followed by endometrial curettage; 31 cases of endometrial adenocarclnoma(CA), 19 cases of endometrial hyperplasia(HP), 83 cases of dysfunctional uterine bleeding(DUB), and 75 cases of normal endometrium. Cervicovaginal smears were reviewed according to the criteria of The Bethesda System. Endometrial cells were identified in 15 of 701 cases(2.1%) in group I and 64 of 208 cases(30.8%) in group II. Among group II, detection rate of endometrial cells was the highest in CA (51.6%) compared to HP(26.3%), DUB(41.0%), and normal endometrium(12.0%) (p<0.05). Cytologic atypia of endometrial cells was not found In group I, but was more frequently identified in CA(87.5%) than in HP(10.5%) or DUB(14.7%) (p<0.05). Exfollatlon of endometrial cells might be related to abnormal endometrial lesion, and reporting of endometrial cells in the cervicovaginal smear may increase a chance to detect endometrial lesions especially in patients with abnormal uterine bleeding.

A Statistic Correlation Analysis Algorithm Between Land Surface Temperature and Vegetation Index

  • Kim, Hyung-Moo;Kim, Beob-Kyun;You, Kang-Soo
    • Journal of Information Processing Systems
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    • v.1 no.1 s.1
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    • pp.102-106
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    • 2005
  • As long as the effective contributions of satellite images in the continuous monitoring of the wide area and long range of time period, Landsat TM and Landsat ETM+ satellite images are surveyed. After quantization and classification of the deviations between TM and ETM+ images based on approved thresholds such as gains and biases or offsets, a correlation analysis method for the compared calibration is suggested in this paper. Four time points of raster data for 15 years of the highest group of land surface temperature and the lowest group of vegetation of the Kunsan city Chollabuk_do Korea located beneath the Yellow sea coast, are observed and analyzed their correlations for the change detection of urban land cover. This experiment based on proposed algorithm detected strong and proportional correlation relationship between the highest group of land surface temperature and the lowest group of vegetation index which exceeded R=(+)0.9478, so the proposed Correlation Analysis Model between the highest group of land surface temperature and the lowest group of vegetation index will be able to give proof an effective suitability to the land cover change detection and monitoring.

Holder exponent analysis for discontinuity detection

  • Sohn, Hoon;Robertson, Amy N.;Farrar, Charles R.
    • Structural Engineering and Mechanics
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    • v.17 no.3_4
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    • pp.409-428
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    • 2004
  • In this paper, a Holder exponent, a measure of the degree to which a signal is differentiable, is presented to detect the presence of a discontinuity and when the discontinuity occurs in a dynamic signal. This discontinuity detection has potential applications to structural health monitoring because discontinuities are often introduced into dynamic response data as a result of certain types of damage. Wavelet transforms are incorporated with the Holder exponent to capture the time varying nature of discontinuities, and a classification procedure is developed to quantify when changes in the Holder exponent are significant. The proposed Holder exponent analysis is applied to various experimental signals to reveal underlying damage causing events from the signals. Signals being analyzed include acceleration response of a mechanical system with a rattling internal part, acceleration signals of a three-story building model with a loosing bolt, and strain records of an in-situ bridge during construction. The experimental results presented in this paper demonstrate that the Holder exponent can be an effective tool for identifying certain types of events that introduce discontinuities into the measured dynamic response data.

Evidence-based Screening, Early Diagnosis and Treatment Strategy of Cervical Cancer for National Policy in Low-resource countries: Example of India

  • Saxena, Upma;Sauvaget, Catherine;Sankaranarayanan, Rengaswamy
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1699-1703
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    • 2012
  • Cervical cancer remains the most frequent cancer in women from the developing world. More than 88% of deaths occur in low-income countries, and it is predicted to climb to 91.5% by 2030. Although Pap-based screening programmes have shown to be effective in reducing the disease burden in high-resource countries, implementation and sustention of cytology-based programmes is quite challenging in low-resource settings. The present paper reviews evidence-based alternatives of screening methods, triaging algorithm, treatment of cervical precancerous lesions, and age-group at screening appropriate for low-income countries. Evidence shows that visual inspection methods using diluted acid acetic or Lugol's iodine, and HPV-DNA testing are more sensitive tests than the Pap-smear screening test. Visual inspection allows an immediate result and, when appropriate, may be immediately followed by cryotherapy, the so called "screen-and-treat" approach, achieved in a single visit, by trained nurses and midwives. Examples of cervical cancer prevention programmes in India and selected low-income countries are given.

Digit Segmentation in Digit String Image Using CPgraph (CPgraph를 이용한 숫자열 영상에서 숫자 분할)

  • Oh, Jeong-su
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.23 no.9
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    • pp.1070-1075
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    • 2019
  • In this paper, I propose an algorithm to generate an input digit image for a digit recognition system by detecting a digit string in an image and segmenting the digits constituting the digit string. The proposed algorithm detects blobbed digit string through blob detection, designates a digit string area and corrects digit string skew using the detected blob information. And the proposed algorithm corrects the digit skew and determines the boundary points for the digit segmentation in the corrected digit sequence using three CPgraphs newly defined in this paper. In digit segmentation experiments using the image group including digit strings printed with a range of the font sizes and the image group including handwritten digit strings, the proposed algorithm successfully segments 100% and 90% of the digits in each image group.

Real-Time Tomato Instance Tracking Algorithm by using Deep Learning and Probability Model (딥러닝과 확률모델을 이용한 실시간 토마토 개체 추적 알고리즘)

  • Ko, KwangEun;Park, Hyun Ji;Jang, In Hoon
    • The Journal of Korea Robotics Society
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    • v.16 no.1
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    • pp.49-55
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    • 2021
  • Recently, a smart farm technology is drawing attention as an alternative to the decline of farm labor population problems due to the aging society. Especially, there is an increasing demand for automatic harvesting system that can be commercialized in the market. Pre-harvest crop detection is the most important issue for the harvesting robot system in a real-world environment. In this paper, we proposed a real-time tomato instance tracking algorithm by using deep learning and probability models. In general, It is hard to keep track of the same tomato instance between successive frames, because the tomato growing environment is disturbed by the change of lighting condition and a background clutter without a stochastic approach. Therefore, this work suggests that individual tomato object detection for each frame is conducted by YOLOv3 model, and the continuous instance tracking between frames is performed by Kalman filter and probability model. We have verified the performance of the proposed method, an experiment was shown a good result in real-world test data.

Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification

  • Yu, Shao-shuai;Che, Hai-yan;Wang, Sheng-jie;Lin, Cai-li;Lin, Ming-xing;Song, Wei-wei;Tang, Qing-hua;Yan, Wei;Qin, Wei-quan
    • The Plant Pathology Journal
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    • v.36 no.5
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    • pp.459-467
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    • 2020
  • Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loop-mediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrI-B subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64℃ for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of disease-resistant Areca palm varieties.