• Title/Summary/Keyword: group detection

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Isolation of Aeromonas sobria Containing Hemolysin Gene from Arowana (Scleropages formosus) (Arowana(Scleropages formosus)에서 Hemolysin Gene을 지닌 Aeromonas sobria 분리 및 특성)

  • Jun, Jin-Woo;Kim, Ji-Hyung;Casiano, Choresca Jr.;Dennis, K. Gomez;Shin, Sang-Phil;Han, Jee-Eun;Park, Se-Chang
    • Journal of Veterinary Clinics
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    • v.27 no.1
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    • pp.62-65
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    • 2010
  • Arowana (Scleropages formosus) is the most valuable group of ornamental fishes and very much in demand in the ornamental fish trade and commands high price ranging from hundreds to thousands of dollars per fish. In this paper, we described a case of mortality of arowana from a private aquarium in Korea. A bacterial pathogen from fish organs (brain, kidney, liver) was cultured, identified and confirmed using Vitek System 2, API 20E test, multiplex PCR and 16S rRNA gene sequencing. The morphological and biochemical properties of the bacterium isolated from the brain, kidney and liver of the fish were similar to Aeromonas sobria. Positive amplification products using the multiplex PCR assay for detection of A. sobria were obtained from these organs. The 16S rRNA gene of the isolates from fish was identical and exhibited 100% sequence similarity with A. sobria (AY987762.1) strain available from GenBank. This bacterium contained hemolysin gene, a virulence factor that plays an important role in outbreaks of disease and is pathogenic to humans as well as in fish. Although this opportunistic bacterium was isolated from a fish without any external symptoms, this pathogen may act as a reservoir and enhance chances of zoonosis to human such as during handling.

Assessment of Allograft Function in Dog Single Lung Transplantation on CT (한국산 잡견에서 컴퓨터단층촬영을 이용한 이식폐의 기능평가)

  • 박기성;박창권
    • Journal of Chest Surgery
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    • v.30 no.11
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    • pp.1055-1061
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    • 1997
  • In the field of the experimental lung transplantation, we analyzed the CT findings of acute rejection, infection in the left single allotransplanted lung of adult mongrel dogs, and the CT findings were compared with the histological findings obtained by the lung biopsy Twenty two adult mongrel dogs were divided into two groups(Donor and recipient group). Donor lungs were flushed with LPDG(low pota,ilium dextral glucose) solution(n=4) or modified Euro-collins solution(n=7) and preserved over 20 hours with $10^{\circ}C(1$ case preservation for 4hours). After left single lung transplantation, the chest X-ray and sequential computed tomogram were performed with concomitant hemodynamic study and arterial blood gas analysis on immediate postoperative period, postoperative 3rd day and postoperative 7th day. Two of eleven transplanted lungs had acute rejection which was represented as moderate infiltration at immediate or 1st postoperative d y but became extensive infiltration at postoperative 3rd day on CT. There were showed one case of bronchopleural fistula, six cases of pneumonia and two cases of pulmonary infarction. In one rejection cases, the opacity of transplanted lung was improved by injection of methylprednisolone 500mg daily during 3 days. We concluded that CT was a useful noninvasive evaluation parameter after lung transplantation and the serial CT scan enabled early detection of acute rejection.

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NERVE DISTRIBUTION OF DENTAL PULP IN HUMAN PRIMARY AND YOUNG PERMANENT TEETH (유치 및 초기 영구치의 치수 신경분포)

  • Lee, In-Jeong;Lee, Jae-Mun;Kim, Hyun-Jung;Nam, Soon-Hyun;Kim, Young-Jin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.23 no.2
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    • pp.291-305
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    • 1996
  • The purpose of this study was to investigate the distribution of nerves in the dental pulp of early extracted primary teeth, normal exfoliated primary teeth, partially-erupted, nonfunctional, premolars, and erupted, functional, premolars. Numbers of sample were 10 teeth in each group. The distribution of nerves in the dental pulp were investigated by means of immunohisto chemistry for detection of neurofilament protein(NFP). The results were as follows: The early extracted primary teeth exhibited patterns of innervation similar to those observed for young permanent teeth. The plexiform arrangement of fibers was not evident in the primary teeth. Most nerves appear to terminate about the odontoblasts. As primary teeth began to undergo root resorption, degenerative changes such as vesicles and fragmentation appear in the nerves. The quantity of neural tissue also decreased. In teeth in which the roots were almost completely resorbed only a small number of nerves remain. There was a decrease in the number of terminal branches in the pulp of the partially erupted, nonfunctional, premolars and those present reached the pulpo-odontoblastic border. The nerve terminals in the pulp of the erupted, functional, premolars were traced to the dentinal tubule and a few nerve fibers formed loops in the predentin.

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Distribution of Abused Drugs in 275 Alcohol-positive Blood Samples of Korean Driver (음주운전자 275명 혈액 중 마약류 및 남용약물의 분석)

  • Choi, Hye-Young;Lee, Ju-Seon;Choi, Sang-Kil;Kim, Eun-Mi;Kim, Jae-Kyun;Kim, Young-Woon;Lim, Mi-Ae;Chung, Hee-Sun
    • YAKHAK HOEJI
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    • v.52 no.2
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    • pp.137-146
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    • 2008
  • Even though driving under the influence of drug (DUID) is a worldwide problem, we, Korea has no regulation system yet except for alcohol, and there are little cases reported related to DUID. In order to investigate the type of abused drugs for drivers in Korea, we tried to analyze controlled and non-controlled drugs in alcohol-positive blood samples. 275 whole bloods, which were positive for alcohol on the roadside test, were collected from the police for two months ($Nov.{\sim}Dec.$ 2006). The analytical strategy was constituted of three steps: First, alcohol in blood samples were confirmed and quantified by gas chromatography. Second, controlled drugs were screened by $Evidence_{investigator}\;^{TM}$ (Randox, U.K.) as preliminary test. It was based on immunoassay by biochip array analyzer. Nine groups of drug abuse were screened: amphetamines, methamphetamines, cannabis, cocaine, opiates, barbiturates, methadone, benzodiazepines I (oxazepam) & II (lorazepam). Finally, confirmation of these drugs was performed by GC-MS. Blood samples were extracted by solid-phase extraction by $RapidTrace^{TM}$ (Zymark, U.S.A.). After trimethylsilyl (TMS) derivatization, eluates were analyzed to GC-MS. Total 49 drugs were investigated in this study including controlled drugs, antidepressants, 1st generation antihistamines, dextromethorphan, nalbuphine, ketamine, etc. For rapid detection, we developed the automated identification system. It was made up a new software, "DrugMan", modified Chemstation data analysis menu and newly developed macro modules. A series of peak selection, identification and reporting of the results were performed automatically by this system. Concentrations of alcohol in 275 blood samples were ranged from 0.011 to 0.249% (average, 0.119%). Among 149 blood samples, just six samples (4.0%) were showed positive results to the immunoassay: one methamphetamine and five benzodiazepines group I. By GC-MS confirmation, only benzodiazepines were detected and methamphetamine was not detected from immunoassay positive blood sample. Besides these drugs, 5 chlorpheniramines, dextromethorphan, diazepam, doxylamine, ibuprofen, lidocaine and topiramate were also detected in whole bloods by GC-MS. Conclusively, the frequency of drug abuse for Korean drivers was relatively low. There was none case which illegal drug was detected. However these results were limited to alcohol positive blood samples, so it is necessary to analyze more samples including alcohol negative blood.

An Adaptive Authentication Protocol for Ambient Assisted Living Systems (전천 후 생활보조 시스템을 위한 적응형 인증 프로토콜)

  • Yi, Myung-Kyu;Choi, Hyunchul;Whangbo, Taeg-Keun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.4
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    • pp.19-26
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    • 2018
  • In recent years, the substantial increase in the population's average age leads to an exceeded number of older persons comparing with the number of any other age group. As a result, both industry and academia are focused on the development of several solutions aimed to guarantee a healthy and safe lifestyle to the elderly. Ambient Assisted Living (AAL) approach is the way to guarantee better life conditions for the aged and for monitoring their health conditions by the development of innovative technologies and services. AAL technologies can also provide more safety for the elderly, offering emergency response mechanisms, fall detection solutions, and video surveillance systems. Unfortunately, due to the sensitive nature of AAL data, AAL systems should satisfy security requirements such as integrity, confidentiality, availability, anonymity, and others. In this paper, we propose an adaptive authentication protocol for the AAL systems. The proposed authentication protocol not only supports several important security requirements needed by the AAL systems, but can also withstand various types of attacks. In addition, the security analysis results show that the proposed authentication protocol is more efficient and secure than the existing authentication protocols.

Benzothiazole Substituted Benzocrown Ether-Based Potassium Ion-Selective Membrane Electrodes (벤조티아졸기를 갖는 벤조크라운에테르를 전극물질로 사용한 $K^+$ 이온선택성 막전극)

  • Hong, Uk Sun;Kwon, Hye Kyong;Cha, Geun Sig;Nam, Hakhyun;Chang, Seung Hyun;Chung, Kwang Bo
    • Journal of the Korean Chemical Society
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    • v.39 no.9
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    • pp.698-704
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    • 1995
  • The ion-selective membrane fabricated with 33 wt% PVC, 66 wt% o-NPOE and 1 wt% 4'-benzothiazolylbenzo-15-crown-5 (TB15C5) as a neutral carrier exhibited an enhanced selectivity to potassium ion over sodium ion compared to those prepared with other 15-crown-5 or 18-crown-6 derivatives. The potentiometric properties (response slope, selectivity, detection limits and lifetime) of TB15C5-based ISE membranes along with those based on valinomycin, benzo-15-crown-5 (B15C5), 4'-aminobenzo-15-crouwn-5 (AB15C5), benzo-18-crown-6 (B18C6), dibenzo-18-crown-6 (DB18C6) and bis[(benzo-15-crown-5)-4'-ylmethyl]pimelate (PI-Ⅱ) ionophores were carefully examined under the same experimental conditions. The enhanced selectivity of TB15C5-based membrane to potassium is explained in terms of the effect of the benzothiazol functional group and the distribution coefficients of metal ions.

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Retrospective Study of the Characteristics and Treatment of Odontomas (치아종의 임상적 특징과 치료에 대한 후향적 연구)

  • Moon, Yujin;Lee, Daewoo;Kim, Jaegon;Baik, Byeongju;Yang, Yeonmi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.2
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    • pp.164-171
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    • 2015
  • This study was aimed to retrospectively evaluate the clinical findings and treatments of odontomas in the Korean demographic group. The subject of the present study included 75 odontomas in 74 patients who received treatment from Chonbuk National University Dental Hospital between April 2005 and March 2014. The average age of the subjects were 10.3 years old (range 3 - 23 years old). 42 (56.8%) males and 32 (43.2%) females were in the present study. It was found that compound odontomas were about 4 times more common than complex odontomas. Odontomas equally occurred in both maxillary and mandible. The impaction of permanent teeth (73.3%) was the most common complication of odontomas on adjacent teeth. Most odontomas (96.0%) were surgically removed either to induce spontaneous eruption or to guide orthodontically to occlusion. When the impacted tooth could not be naturally or forcefully erupted to occlusion, it was extracted. Early detection and treatment of odontomas would increase the possible preservation of the impacted tooth by eliminating the source of disturbance. Therefore, this study recommends that periodic radiographic examination from birth until the eruption of the permanent third molar to prevent possible hindrance to the teeth eruption.

Prediction Model for Toothache Occurrence in College Students by using Oral Hygiene Habits and the CART Model (대학생의 구강건강관리실태와 CART모델을 이용한 치통발생예측)

  • Kim, Nam-Song;Lim, Kun-Ok
    • Journal of dental hygiene science
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    • v.9 no.4
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    • pp.419-426
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    • 2009
  • The occurrence of toothache signals the malfunction in oral health, which allows the detection of any abnormal condition in the oral cavity at an early stage to prevent the condition from worsening, and thus can act as a preventive measure. This study has looked into the status of oral health management in relation to toothache through the structured survey administered to 235 college students. Based on the survey results, this study aimed at comparing the toothache occurrence prediction between regression analysis and CART model in order to clarify the relationship between the factors of oral health management habits that contribute to toothache occurrence. According to the result, there was a difference between the present health status and the health status of the past year depending on the presence or non-presence of toothache occurrence (p<0.05). There was a difference in the regularity of meal time depending on the presence non-presence of toothache occurrence from the dietary habits of the research subjects (p<0.05). As for the presence or non-presence of toothache occurrence from the oral hygiene habits of the research subject, there was a difference between the occurrence and nonoccurrence of bleeding during brushing or flossing (p<0.05). According to the results of regression analysis, no factors were signifiant in the relationship with the presence or non-presence of toothache occurrence from the status of life habits and oral hygiene habits. 70% of the researched group was randomly selected as the sample for generating an analytical model and the remaining 30% was used as the sample for generating an evaluation model. According to the results of CART model, the occurrence of toothache was higher in the case of irregular meal time and poor current health condition than the case of average or satisfactory health condition. The above results imply that CART model is very useful technique in predicting toothache occurrence compared to regression analysis, and suggests that CART model could be very useful in predicting other oral diseases including toothache.

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Monitoring on Benzo(a)pyrene Content in Oriental medicine (유통 한약재 중 벤조피렌 함유량에 관한 모니터링)

  • Lee, Mi-Yeong;Jung, Sang-Mi;Lee, Gye-Won
    • Journal of Digital Convergence
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    • v.10 no.7
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    • pp.201-206
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    • 2012
  • Benzo(a)pyrene is a polycyclic aromatic hydrocarbons (PAHs) whose metabolites are mutagenic and highly carcinogenic and is listed as a Group 1 carcinogen by the IARC. It has been found at variable concentrations in several foods and is associated with several factors during the process including contaminated raw materials, exposure of environment, and procedure of process or cooking. In this study, benzo(a)pyrene in 45 oriental medicines were determined by HPLC/FLD. The calibration curves of benzo(a)pyrene was linear over the concentration range of 0.5~40 ng/mL with correlation coefficient of above 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) of benzo(a)pyrene were 0.04 and 0.10 ${\mu}g/kg$. Benzo(a)pyrene in 3 samples out of 45 samples was not detected. The level of benzo(a)pyrene in 26 (57.7%), 8 (17.8%) and 7 (15.6%) samples was 0.1~0.5, 0.5~1.0 and 1.0~5.0 ${\mu}g/kg$, respectively. Especially, content of benzo(a)pyrene in Coptis Rhizome is the highest (5.97 ${\mu}g/kg$). In conclusion, these results suggest that could be applied to fundamental study and guideline on drying condition to decrease content of benzo(a)pyrene in oriental medicine.

The Development and Effect of Navigator Education Program for Cancer Screening on Women in the Community (지역사회 여성암 검진 네비게이터 교육 프로그램 개발 및 효과 분석)

  • Lee, Bo-Young;Jo, Heui-Sug;Lee, Hey-Jean
    • Journal of agricultural medicine and community health
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    • v.34 no.2
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    • pp.214-222
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    • 2009
  • Objectives: This study was performed to evaluate the effect of navigator education program for cancer screening, which is designed for improvement in knowledge of cancer, perceived self efficacy and communication skill of the breast and cervical cancer screening for middle-aged and aged women in urban areas. Cancer screening navigator is lay health advisor who are educated for providing information, emotional support about cancer screening at the community. Methods: The subjects were 33 women at the age of 40-69 and educated for 12 hours through the education program. The control group subjects were 30 women. For statistical analysis, descriptive statistics and paired t-test were used with SPSS WIN 14.0. Results: Contents of education program were case of cancer early detection, benefit of breast cancer screening, benefit of cervical cancer screening, health care system for cancer screening, role of cancer screening navigator, communication skill, transtheoretical model and role play. Knowledge of cancer(t=4.267, p=0.000) and communication skill(t=4.947, p=0.000) of the women increased significantly after implementing the 12 hours education program. Conclusion: The results suggest that navigator education for cancer screening has an effect in increasing knowledge of cancer, and communication skill scores.