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Xylan으로부터 단리한 Glucuronic Acid가 유산소 운동 후 흰쥐 백근의 항산화계에 미치는 영향 (Effect of Glucuronic Acid Derivertives Isolated from Xylan on Antioxidative Defense System in Rat White Gastrocnemius after Aerobic Exercise)

  • 김관유;이순재
    • Journal of Nutrition and Health
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    • 제35권7호
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    • pp.729-736
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    • 2002
  • The purpose of this study was to investigate the effects of glucuronic acid (isolated from xylan) on antioxidative defense system in rat after aerobic exercise. The glucuronic acid was isolated from xylan. Sprague-Dawley male rats weighing 150$\pm$10 g were randomly assigned to one normal group and three exercise training groups. Exercise training groups were classified to T (glucuronic acid free diet), TU (250 mg glucuronic acid/kg bw) and 2TU (500 mg glucuronic acid/kg bw) according to the level of glucuronic acid supplementation before exercise training. The experimental rats in exercise training groups (T, TU and 2TU) were exercised on glucuronic acid supplementation or rats in normal group (N) were confined in cage for 4 weeks. And rats were sacrificed with an overdose of pentobarbital injection just after running. Body weight, food intakes and food efficiency ratio (FER) were lower in the exercise training group than in the normal group. White gastrocnemius xanthine oxidase (XOD) activity in the T group was 85% greater than that of the normal group, whereas in the TU and 2TU groups it did not differ from the normal group. White gastrocnemius superoxide dismutase (SOD) activity in T group, that was decreased by 22% compared with that of N group, but those of TU and 2TU groups were increased by 38% and 42%, respectively, compared with that of T group. White gastrocnemius glutathione peroxidase (GSHpx) activity in T group, that was decreased by 42% compared with that of N group, but those of TU and 2TU groups were increased by 67% and 68%, respectively, compared with that of T group. Glutathione S-transferase (GST) activity of white gastrocnemius in N group was not significantly different from that in the T and TU groups, but 2TU group were increased by 12%. Contents of thiobarbituric acid reactive substance (TBARS) in T group was increased by 54%, compared with that of normal group but those of TU group and 2TU group were lower 44% and 36% than that of T group. In conclusion, the effects of glucuronic acids in exercise training rats would appear to reduce peroxidation of tissue as an antioxidative defense mechanism.

종양세포(腫瘍細胞)의 염색체(染色體)에 대한 오크라톡신 A의 독성(毒性)에 관한 연구(硏究) (Studies on toxicity of ochratoxin A to chromosomes of turmor cell-line)

  • 윤화중;노민희;김강련
    • 대한수의학회지
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    • 제29권2호
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    • pp.51-57
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    • 1989
  • This study was performed to investigate the toxicity of ochratoxin A (OA) to the chromosomes of $K_{562}$ tumor cell-line in vitro. The results of this experiment were as follows: 1) Chromosomes of $K_{562}$tumor cell-line resulted in pseudotriploidy on the control group. Chromosomes of $K_{562}$ tumor cell-line treated with OA resulted in heteroploidy compared with the control group. The mean number of chromosomes in the karyotype of the control group (60) were 7 in the A group, 5 in the B group, 20 in the C+X group, 7 in the D group, 9 in the E group, 6 in the F group, and 6 in the G+Y group respectively. The number of chromosomes were increased as follows: Treating with $0.7{\mu}M$ OA, the number of chromosomes were increased one in E and F group, two in G+Y group compared with control group. In treated with $1.5{\mu}M$ OA, the increasing number of chromosome was one in E and F group. In treated with $3{\mu}M$ OA, E and F group was increased one and G+Y group were increased two chromosomes compared with control group. But in treated with $6{\mu}M$ OA, the number of chromosome in G+Y group was decreased one. 2) $K_{562}$ tumor cell line treated with OA showed Philadelphia-Chromosome in the long arm of the G group karyotype chromosome. The rate of chromosome aberration in $K_{562}$ tumor cell-line treated with OA was 77% in $0.7{\mu}M$ OA group, 71% in $1.5{\mu}M$ OA group, 82% in $3{\mu}M$ OA group and 94% in $6{\mu}M$ OA group respectively. The rate of chromosome aberration of $K_{562}$ tumor cell-line treated with OA was high in the high dose level of OA, and chromosome aberration of $K_{562}$ tumor cell-line treated with OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype. As a result of this study, the toxicity of OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype, and then, the toxicity of OA resulted in the damage to RNA and protein synthesis in $K_{562}$ tumor cell-line, and the C-group karyotype of $K_{562}$ tumor cell-line was target of the toxicity of OA.

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해결중심 집단상담이 비행청소년의 스트레스 반응과 대처방식에 미치는 효과 (The Effects of Solution-Focused Group Counseling on the Stress Response and Coping Strategies in the Delinquent Juveniles)

  • 고미자;유숙자;김양곤
    • 대한간호학회지
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    • 제33권3호
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    • pp.440-450
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    • 2003
  • Purpose: This study was conducted to examine the effects of solution-focused group counseling on the general stress, stress response and coping in the delinquent juveniles. Method: The subjects consisted of a convenience samlple of 60 delinquent juveniles who had been placed under probation in Kwang-ju, and recruited from Oct., 2000 to Dec., 2000. Solution-focused group counseling was conducted for the experimental group once a week for 120 to 180 minutes for 6 week. The control group received no group session. For both groups the level of general stress, stress response and coping were measured before and after the experiment. Result: General stress significantly decreased in the experimental group and increased in the control group, but showed no significant difference between the two groups. Stress response decreased significantly in the experimental group and increased in the control group, but showed no significant difference between the two groups. The problem-focused coping significantly increased in the experimental group and decreased in the control group, and showed a significant difference between the two groups. The emotion-focused coping significantly decreased in the experimental group and increased in the control group, and showed no significant difference between the two groups. Conclusion: This study showed that solution-focused group-counselling has a positive effect on variables like the general stress, stress response, emotion-focused coping and problem-focused coping. Further study needs to be conducted to verify results.

The Effects of Whole Body Vibration Exercise on Jump Performance and Quadriceps Muscle Activation in Soccer Player

  • Kim, Je-Ho
    • The Journal of Korean Physical Therapy
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    • 제27권3호
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    • pp.129-134
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    • 2015
  • Purpose: The purpose of this study was to determine the influence of WBV exercise on CMJ and quadriceps muscle activation according to different frequency of vibration in soccer player and also to find effective frequency for leading appropriate treatment reaction. Methods: Thirty three subjects were randomly divided into three groups: the three groups are WBV group using 20 Hz frequency, WBV group using 30 Hz frequency and squat exercise group according to training method. The exercise program was conducted for six weeks. Subjects were measured on CMJ and quadriceps muscle activation. Results: Significant difference in CMJ was observed in the group I, II compared with the group III (p<0.05). Results of post-hoc, showed a significant difference in CMJ in on group I, II compared with the group III, but no a statistically significant difference between group I and II. Significant difference in quadriceps muscle activation was observed in the group I, II compared with the group III (p<0.05, p<0.01). Results of post-hoc, significant difference in quadriceps muscle activation in on group I, II compared with the group III and significant difference between group I and group II. Conclusion: This research intervened WBV for soccer players and compared the differences of CMJ and quadriceps muscle activation; as a result of the effective frequency for improving performance, there is a significant difference in CMJ and quadriceps muscle activation of WBV group with comparison of control group; and it was proved that WBV is effective using 30 Hz frequency for improving quadriceps muscle activation.

곡류중(穀類中)의 미량원소(微量元素)에 관(關)한 연구(硏究) (Studies on Trace Elements in Cereals)

  • 홍영숙;신정래
    • Journal of Nutrition and Health
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    • 제8권1호
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    • pp.39-46
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    • 1975
  • This studies were designed to know the contents of trace element and magnesium in cereals. Twenty two kinds of cereal, 104'samples, were analyzed by atomic absorption spectrophotometer for the determination of Manganese, Iron, Magnesium, Chrome, Cupper, Nickel, Zinc, Stannous, Lead and Cadmium. The results are as follows: 1) The contents of Mn and Fe in the sesame group show highest value and next is bean group. In the rice group its content shows lowest value than other cereals. 2) In the Cr content its value is lower than other elements in cereals respectively. Cr content of sesame and bean group show higher than rice and barley group. 3) The contents of Cu and Ni in the sesame and bean group show higher value than the value of rice and barley group. Its contents of rice and barley group is almost equal in Cu and Ni. 4) For the Zn content the value of sesame group is higher than other cereals and next is bean group. In the Sn content the value of bean group shows higher one than other cereals. The contents of Zn and Sn in rice and barley group are lower than other cereals. 5) The value of Pb is almost equal to each cereal. The content of Cd in bear group and barley group show higher than other cereals and its value of rice group is equal to the value of barley group.

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복합(複合) resin의 변록누출(邊綠漏出)에 관(關)한 실험적(實驗的) 연구(硏究) (A STUDY ON THE MARGINAL LEAKAGE OF COMPOSITE RESIN)

  • 조진호
    • Restorative Dentistry and Endodontics
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    • 제7권1호
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    • pp.131-138
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    • 1981
  • The purpose of this study was to evaluate the marginal leakage of composite resin. Preparing 144 class V cavities on freshly extracted noncarious teeth, composite resins were prepared and inserted by one dentist according to the manufacturer's instructions. The experiments were performed in two different groups; In group I; Class V cavities with $90^{\circ}$ cavosurface angle, In group II; Class V cavities with $135^{\circ}$ cavosurface angle. And each group was divided 2 subgroups; In control group; composite resin restoraions without acid etch technique. In experimental group; composite resin restorations with acid etch technique. All specimens were immersed in 0.05% crystal violet solution. Before examination, the restored teeth were subjected to thermal stress. The specimens were sectioned occlusogingivally through the center of the restorations with a diamond disk. The sections were examined under a reflected light microscope at 1 day, 7 days and 30 days after immersing the specimens in dye solution. The results were as follows; 1. Control group of group I and group II showed marginal leakage. 2. The degree of marginal leakage in experimental group was greater reduced than control group. 3. In control group, the degree of marginal leakage in group I was greater than group II. 4. In experimental group, there is not statistical differences of the degree of marginal leakage between group I & group II.

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구강내 연조직 질환과 구강점막 각화도와의 관계 (A Study of the Relationship between Keratinization of Oral Mucosa and Intraoral Soft Tissue Disease)

  • 송주종;김병국;최홍란
    • Journal of Oral Medicine and Pain
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    • 제26권1호
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    • pp.1-10
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    • 2001
  • To investigate the relationship between several intraoral soft tissue lesions(hairy tongue, lichen planus, recurrent aphthous stomatitis, oral candidiasis, glossitis and oral herpetic lesion) and oral mucosal keratinization, exfoliative cytological smear on intraoral mucosal surfaces were performed on each number of patients and 25 controls keratinization cell (yellow-stained cell) ratio was then measured. In hairy tongue, there was no significant difference between patient group and control group in all kind of cells. Only blue cell ratio of women was more than of men in patient group. In lichen planus, there was no difference between patient and control group in yellow cell ratio. Red cell ratio in the control group was more than in the patient group. Blue cell ratio in the patient group was more than that in control group. But there was no sex predilection between both groups in the ratio of all kind of cells. In recurrent aphthous stomatitis, Yellow cell ratio in the control group was more than that in the patient group. Red cell ratio in the control group was more than that in control group. Blue cell ratio in the patient group was more than that in control group. But there was no sex predilection between both groups in the ratio of all kind of cells. In oral candidiasis, Yellow cell ratio in the control group was more than that in the patient group. Red cell ratio in the control group was more than that in control group. Blue cell ratio in the patient group was more than that in control group. There was no sex predilection between both groups in yellow cell ratio. Red cell ratio of women was more than of men in patient group. Blue cell ratio of men was more than of women in patient group. In herpetic lesions, there was no difference between patient and control group in yellow cell ratio. Red cell ratio in the control group was more than in the patient group. Blue cell ratio in the patient group was more than that in control group. Yellow cell ratio of women was more than of men in control group. Red cell ratio of men was more than of women in control group. Blue cell ratio of men was more than of women in patient group. In glossitis, Yellow cell ratio in the control group was more than in the patient group. There was no difference between patient and control group in red cell ratio. Blue cell ratio in the patient group was more than that in control group. Yellow cell ratio of women was more than of men in control group. Red cell ratio and blue cell ratio of men were more than of women in control group. According to above results, the ratio of keratinized cell in atrophic, ulcerated, or pseudomembranous lesions was lowered than in control, but the ratio of keratinized cell in keratotic, vesicular or lesions on keratinized surface lesions had no difference to control group. Thus, keratotic, vesicular or lesions on keratinized surface lesions have not closely relation to mucosal keratinization. And, there was a little sex predilection between men and wemen in mucosal keratinization.

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익위탕(益胃湯)의 Indomethacin유발 위점막 손상에 대한 효과 (The Effects of Ikwitang Extract on Indomethacin-induced Gastric Mucosal Lesions)

  • 백태현;신혜승
    • 대한한방내과학회지
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    • 제25권3호
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    • pp.497-507
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    • 2004
  • This study was carried out to investigate the effects of Ikwitang extract on indomethacin-induced gastric mucosal lesions in mice. Experimental groups were classified into a non-treated group(NM group), a non-administered group(CON group), the Misoprostol-administered group(MI group) and the Ikwitang-extract-administered group(IW group). This study examined the morphological change, distribution of mast cells, mucous surface cells, acid mucose secreted cells, and apoptic cells, BrdU, COX-1, Hsp70, $NF-{\kappa}B\;p50$, PKC, COX-2 and IL-12B of gastric mucosa. Results : The results of this study were as follows: 1. In the Misoprostol-administered group and the Ikwitang-extract-administered group, the hemorrhagic erosion of gastric mucous and infiltrated mast cells decreased. 2. Mucous surface cells and acid mucose secreted cells abserved in the Misoprostol-administered group and the Ikwitang-extract administered group. 3. The distribution of apoptic cells, Hsp70, $NF-{\kappa}B\;p50$, PKC, COX-2, and IL-12B increased in the Control group, but decreased in the Misoprostol-administered group and Ikwitang-extract-administered group. 4. Cells proliferation of gastric mucosa and the COX-l positive cells decreased in the Control group, but increased in the Misoprostol-administered group and the Ikwitang-extract -administered group. The above results suggest the lkwitang extract had beneficial effects on indomethacin induced gastric mucosal lesions.

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컴퓨터 보조 수업 (CAI)의 학습효과에 관한 연구 -중학교 가정과 디자인의 선택 단원을 중심으로 (A Study on the Learning Effect of Computer Assisted Instruction (CAI) in the Home Economics Class of Middle School)

  • 윤지현;신상옥
    • 한국가정과교육학회지
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    • 제5권1호
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    • pp.143-152
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    • 1993
  • The purposes of this study were to compare the learning effects of CAI and traditional instruction method in the Home Economics class of middle school and to examine the difference of learning effect between CAI group and traditional instruction group according to ability level. The research findings are as follows: 1.$\circled1$As a result of the post test, CAI group showed significantly higher scores on academic achievement than those of traditional instruction group(p<.05). $\circled2$As a result of the retention test, CAI group showed higher scores than those of traditional instruction group, but statistically the difference is not so significant. 2. $\circled1$Higher group in pre-test score of CAI group showed higher scores in post-test and retention test than those of traditional instruction group, but statistically the difference is not so significant. $\circled2$Lower group in pretest score of CAI group showed significantly higher academic achievements than those of traditional instruction group(p<.05). $\circled3$Higher group in IQ test score of CAI group did not show significantly higher academic achievements than those of traditional instruction group. $\circled4$Lower group in IQ test score of CAI group showed significantly higher academic achievements than those of traditional instruction group(p<.05)

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맥문동 물 추출물의 식이가 Streptozotocin으로 유도한 당뇨 흰쥐의 혈당과 혈청 콜레스테롤 함량에 미치는 영향 (Dietary Effect of Liriopis Tuber Water Extracts on the Level of Blood Glucose and Serum Cholesterol in Streptozotocin-Induced Diabetic Rat)

  • 임정교;강명수;박인경;김순동
    • 동아시아식생활학회지
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    • 제15권1호
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    • pp.20-28
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    • 2005
  • The dietary effect of water extracts of Liriopis tuber(WELT) in the diabetic SD-rats on the level of blood sugar and serum cholesterol was investigated. The experimental plots divided into normal group(N), diabetic control(DC), 5% WELT-group(WELT-I) and 10% WELT-group (WELT-II). Each group was fed for 6 weeks, then continuously fed for 1 more week after streptozotocin injection. The loss of the body weight fed for one week after induction of the diabetes was 2.2~6.3% in the WELT-I and -II groups, but it was 18.6% in the DC-group. There was no significant difference in the feed intakes after diabetes induction between N-group and WELT-group, while it was significantly increased in DC-group. The feed efficiency ratios before diabetes induction were 1.70 in WELT-I group, 1.53~1.59 in the N, DC and WELT-II group, while the ratios after diabetes induction were 0.92 in DC-group, 1.51~1.83 in the N, WELT-I and -II group. While the amounts of water intakes for one week after diabetes induction was 625.4 mL in the DC-group, and it were 364.3~371.1 mL in the WELT-groups showing no significant difference with N-group. The excretion amounts of urine were 431.96 mL in DC-group for one week after diabetes induction, and it was 182~192.84 mL in WELT-groups. The ratios of liver weight against body weight were 2.74% in N-group, 2.93~2.96% in WELT-groups, but it was 4.01% in DC-group. The level of blood glucose in WELT-groups fed for one week after diabetes induction were 136.8~138.6 mg/dL showing no significant difference with N-group, but it was 357.8 mg/dL in DC-group. The level of serum triacylglycerol and serum total cholesterol were 93.8 and 68.7 mg/dL in N-group, 120.1 and 101.6 mg/dL in DC-group, 97.4~100.6 and 60.8~67.7 mg/dL in WELT-groups, respectively, showing no significant difference between N-group and WELT-groups. HDL-cholesterol/total cholesterol ratio were 0.63 in N-group, 0.57~0.67 in WELT -groups, which was significantly higher than that of DC-group(p<0.05). Atherogenic index were 0.58 in N-group, 0.49~0.74 in WELT-groups, but it was 1.32 in DC-group. The above results suggest that the WELT diets may have both preventive and curing effects against the diabetes.

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