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Effects of Ethanol Extracts from Commonly Consumed Vegetables by Koreans on NO, TNF-${\alpha}$ and MCP-1 Production in LPS-stimulated RAW 264.7 Macrophages (한국인 다소비 채소의 에탄올 추출물이 LPS 처리된 대식세포에서 NO, TNF-${\alpha}$와 MCP-1 분비에 미치는 영향)

  • Ahn, Eun Mi;Kang, Hyun Ju;Park, Young-Hee;Kim, Young;Kang, Min-Sook
    • Journal of the East Asian Society of Dietary Life
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    • v.24 no.6
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    • pp.776-784
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    • 2014
  • Korean dishes, Hansik are characterized by healthful vegetable intake. The purpose of this study was to evaluate the inhibitory effect of commonly consumed vegetables by Koreans on obesity/metabolic disease-related inflammation. Through statistical analysis of the KNHANES database ($1^{st}$ 1998, $5^{th}$ 2010, 2011) and a literature review, we selected vegetables for study. Among the vegetables, main or sub ingredients of Kimchi were excluded. Samples were prepared using only edible portions and freeze-dried. After grinding, samples were extracted with ethanol, evaporated and finally lyophilized. The cytotoxicity of samples was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, at various concentrations that do not affect cell viability. Raw 264.7 macrophages were treated with lipopolysaccharide (LPS) and 11 kinds of samples or positive control (troglitazone) dissolved in dimethyl sulfoxide (DMSO). After 24 hours, nitric oxide (NO), tumor necrosis factor ${\alpha}$ (TNF-${\alpha}$) and monocyte chemoattractant protein-1 (MCP-1) production were determined. Excepts for young pumpkin and bracken, nine samples effectively reduced NO production compared with control treated with LPS and DMSO. NO levels of five samples (bean sprouts, leeks, eggplant, mugwort, and pumpkin) were similar to that of the positive control. These five samples showed significantly decreased TNF-${\alpha}$ or MCP-1 compared to the control group. Our results suggest that consumption of commonly consumed vegetables contributes to partial prevention of obesity and related metabolic syndrome through reduction of NO, TNF-${\alpha}$, and MCP-1 production.

Hyaluronidase inhibitory activity of extracted phenolic compounds from ultrafine grind Saururus chinensis (초미세 분쇄한 삼백초로부터 추출된 phenolic 화합물의 hyaluronidase 억제 효과)

  • Park, Ki-Tae;Oh, Sang-Lyong;Cho, Young-Je
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.20-26
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    • 2016
  • In this study, the anti-inflammatory effect of hyaluronidase (HAase) inhibition was determined from 92 species of oriental herbal medicine extracted with water and ethanol solvents because of their non-toxicity in the human body. The water extracts of Evodia officinalis (86.8%), Thuja orientalis (80.8%), Carthami semen (66.5%), Melia azedarach (74.7%), Siegesbeckia pubescens (61.3%), Saururus chinensis (49.15%) showed a relatively greater anti-inflammatory activity. The ethanol extracts of Ailanthus altissima and Saururus chinensis demonstrated the highest anti-inflammatory effect at above 90%. Saururus chinensis was selected for its high anti-inflammatory effect in both water and ethanol extract. Ethanol was more effective than water and optimal extraction conditions for phenolic compounds was determined to be extraction with 50% ethanol for 12 hours. The extracts from Saururus chinensis in optimal condition showed 70~80% anti-inflammatory effect when $100{\sim}250{\mu}g/mL$ phenolic concentration was treated. Concentration of above $500{\mu}g/mL$ decreased the inhibitory effect. The anti-inflammatory effect and extraction yield were increased by ultra-fine grind technology, indicating that this method can be used to increase the extraction yield of phenolic compounds from medicinal plants.

New Magnetic Phases of Fe-N and Mn-Al Alloys Produced by Mechanochemical Milling (기계적 밀링 및 화학적 추출법에 의해 제조한 Fe-N 및 Mn-Al계의 새로운 자성재료)

  • Kyu-Jin Kim;Tae-Hwan Noh;Kenji Suzuki
    • Journal of the Korean Magnetics Society
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    • v.4 no.4
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    • pp.347-354
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    • 1994
  • The structural change and magnetic properties of mechanically milled Fe-N and Mn-Al alloy powders have been investigated by XRD, TEM, VSM, $M\"{o}ssbauer$ spectroscopy and inelastic neutron scattering measurements. During milling of ${\gamma}'-Fe_{4}N$ powders, and fcc ${\gamma}'-Fe_{4}N$ phase is transformed to a bct ${\alpha}'-Fe(N)$ phase by stress-induced martensitic transformation, being accompanied by an initial increase in saturation magnetization. During annealing the bct ${\alpha}'-Fe(N)$ nanocrystalline phase which is obtained by mechanical grinding for a long time, an ${\alpha}'-Fe_{16}N_{2}$ phase partially appears as an intermediate phase at 673~773 K, causing an increase in saturation magnetization. During milling of Mn-45, 70 and 85 at.% Al mixed powders, Al atoms are partially solubilized into an ${\alpha}-Mn$ phase. The Al supersaturated ${\alpha}-Mn-type$ phases change from paramagnetic to ferromagnetic : the saturation magnetization is 11 emu/g for the as-milled Mn-70 at.% Al powders. Moreover, by removing almost all Al atoms from the as-milled Mn-85 at.% Al powders using chemical leaching, the saturation magnetization increases up to 36 emu/g. The above bct ${\alpha}'-Fe(N)$ and ferromagnetic ${\alpha}-Mn$ type alloys are the magnetic materials found for the first time, by using the present mechanochemical process.

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Exchange Decoupling Of $Fe_3$Fe_3B+Nd_2Fe_{14}B Spring Magnet Powder Compact (Fe_3B+Nd_2Fe_{14}B Spring magnet분말 압분체의 Exchange Decoupling)

  • 한종수;양충진;박언병
    • Journal of the Korean Magnetics Society
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    • v.11 no.5
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    • pp.232-238
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    • 2001
  • Experimentally it is well known that the magnetic properties significantly deteriorate when nanocomposite bonded magnet are made from nanocomposite ribbon. A decrease in maximum energy product of F $e_3$B+N $d_2$F $e_{14}$B nanocomposite from 14 MGOe in nanocomposite ribbon to 6.5 MGOe in powder compact was fecund to be general. Thus, the present study is focused on finding out the root of exchange decoupling of N $d_4$F $e_{73.5}$ $Co_3$H $f_{0.5}$G $a_{0.5}$ $B_{18.5}$ nanocomposite powder compacts. The exchange decoupling behavior of the powder compact of F $e_3$B+N $d_2$F $e_{14}$B composition was studied by measuring DC demagnetization and isothermal remanent demagnetization curves, which are essential for plotting produced $\delta$M curve. From the $\delta$M plot the deterioration in the magnetic properties resulted from the fact that the magnetostatic interaction became dominant rather thanthe exchange interaction in powder compact. It is concluded that the demagnetization behavior governed by the dominant magnetostatic interaction reduced the remanence magnetization, which caused the reduction of maximum energy Product of the powder compact. We also found that the elimination of residual stress which is unavoidably accumulated during grinding process enhanced the magnetic properties considerably.bly.bly.

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Influence of the homogenizing grade and meathematical treatment on the determination of ground beef components with near infrared reflectance spectroscopy (식품의 근적외선 반사분광분석법에서 균질의 정도가 흡광도에 미치는 영향 및 수학적 처리방법에 관한 연구)

  • Oh, Eun-Kyong;Grossklaus, Dieter
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.408-413
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    • 1992
  • This study was conducted to determine the effect of the homogenizing grade of sample on absorbance of near infrared reflectance spectrophotometer with which chemical compositions of food were rapidly and effectively analyzed. By the mathematical treatment of absorbance values standard error of prediction was reduced as follows. 1. The absorbance values of various samples ground for the same periods of time were calibrated before or after treatment with first or second derivative in an attempt to accurately predict the components of samples ground for the different periods of time. The standard error of prediction for moisture content were 1.478%, 0.658% and 0.580%, respectively, those for fat content 0.949%, 0.637% and 0.527%, respectively, and those for protein content 0.514%, 0.493% and 0.394%, respectively. Calibration of absorbance values after second derivative treatment showed the highest accuracy in predicting sample components. 2. The absorbance values of various samples ground for the different periods of time were calibrated before or after treatment with first or second derivative in order to accurately predict the components of samples ground for the different periods of time. The standard error of prediction for moisture content were 1.026%, 0.589% and 0.568%, respectively, and those for protein content 0.860%, 0.557% and 0.399%, respectively. The standard error of prediction were lower in the order of calibrations before and after first and second derivative treatments. As a result, calibration of absorbance values after second derivative treatment showed higher accuracy regardless of grinding time of samples.

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Gravity Separation Characteristic for the Gold.Silver Ores on the Philippine Mankayan District (필리핀 만카얀 지역 금.은 광석의 비중선별 특성)

  • Kim, Hyung-Seok;Chae, Soo-Chun;Kim, Jeong-Yun;Sohn, Jeong-Soo;Kim, Sang-Bae
    • Journal of the Mineralogical Society of Korea
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    • v.21 no.4
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    • pp.383-395
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    • 2008
  • To enhance the grade and recovery rate of the gold/silver ores which yield at Philippine Mankayan mine, we studied the characteristics which are the geologic and mineralogical features of gold and silver ore, the liberation by crushing and grinding, the separation by sieving and shaking table. Gold/silver ore is composed of the sulfide minerals like pyrite, sphalerite, galena; and the gangue minerals which is quartz, clay. Gold/silver element are mainly contained in a sulfide minerals like pyrite, sphalerite and galena. To increase the liberation rate of sulfide minerals containing gold/silver element, the gold/silver ore has to be grounded under $100{\mu}m$ very finely because the crystal size of sulfide minerals is distributed from $1{\mu}m$ to $100{\mu}m$. The liberation rate of gold/silver ore increases to 92% when the particle size ($d_{90}$) of ore is grounded below $100{\mu}m$ by jaw crusher $\to$ cone crusher $\to$ rod mill by steps. The grade and recovery of sulfide minerals could not be enhanced by sieving separation because those crystal size is distributed homogeneously below $100{\mu}m$. But, when we separated the sieved ore using shaking table, the gold and silver grade increased to 40 ppm and 140 ppm, respectively. Then the recovery rate of gold reach almost 100% but that of silver is no more that 50%.

Flotation for Recycling of a Waste Water Filtered from Molybdenite Tailings (몰리브덴 선광광미 응집여과액 재활용을 위한 부유선별 특성)

  • Park, Chul-Hyun;Jeon, Ho-Seok;Han, Oh-Hyung;Kim, Byoung-Gon;Baek, Sang-Ho;Kim, Hak-Sun
    • Journal of the Mineralogical Society of Korea
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    • v.23 no.3
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    • pp.235-242
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    • 2010
  • Froth flotation using the residual water in the end of flotation process has been performed through controlling of pH. IEP (isoelectric point) of molybdenite and quartz in distilled water was below pH 3 and pH 2.7, respectively and the stabilized range was pH 5~10. In case of a suspension in reusing water, zeta potential of molybdenite decreased to below -10 mV or less at over pH 4 due to residual flocculants. As result of pH control, flotation efficiency in the alkaline conditions was deteriorated by flocculation, resulting from expanded polymer chain, ion bridge of the divalent metal cations ($Ca^{2+}$), and hydrophobic interactions between the nonpolar site of polymer/the hydrophobic areas of the particle surfaces. However, the weak acid conditions (pH 5.5~6) improved the efficiency of flotation as hydrogen ions neutralize polymer chains and then weakened its function. In cleans after rougher flotation, the Mo grade of 52.7% and recovery of 90.1% could be successfully obtained under the conditions of 20 g/t kerosene, 50 g/t AF65, 300 g/t $Na_2SiO_3$, pH 5.5 and 2 cleaning times. Hence, we developed a technique which can continuously supply waste water filtered from tailings into the grinding-rougher-cleaning processes.

MORPHOLOGICAL CHANGES OF DENTIN SURFACE TREATED WITH VARIOUS DENTIN SURFACE CONDITIONERS (수종(數種) 표면처리제(表面處理劑)에 의(依)한 상아질(象牙質) 표면(表面)의 형태(形態) 변화(變化)에 관(關)한 연구(硏究))

  • Cho, Jin-Ho;Choi, Ho-Young;Min, Byung-Soon;Park, Sang-Jin
    • Restorative Dentistry and Endodontics
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    • v.13 no.2
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    • pp.323-334
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    • 1988
  • The purpose of this study was to observe the effect of dentin surface conditioners on the dentin surfaces. Freshly extracted human molars were used in this study. They were stored at $4^{\circ}C$ saline solution before experiment. The crown portions of the teeth were cut in various directions by means of wet diamond point to expose dentin which include transverse, vertical oblique, horizontal and oblique cut to the long axis (Fig. 1). Each tooth was then mounted with self curing acrylic resin in brass ring to expose the flattened dentin surfaces. Final finish was accomplished by grinding the dentin specimens with wet No. 180 and No. 600 grit silicon carbide abrasive paper until a 6.0mm in diameter on a dentin surface was exposed without pulp exposure. The specimens were divided into 9 groups according to the modes of dentin treatment procedure. The following surface treatments were applied on these preparation surfaces; Group 1: unetched (control group) after finish with No. 600 silicon carbide abrasive paper. Group 2: etched with 30% phosphoric acid for 60s Group 3: etched with 10-3 solution for 60s Group 4: Cleaned with 5% NaOCl for 30s Group 5: applied Dentin Adhesit Group 6: cleaned with 5% NaOCl followed by applying the Dentin Adhesit$^{(R)}$ Group 7: applied Photo Bond on the unetched dentin followed by applying the Photo Clearfil Bright Group 8: Etched with 30% phosphoric acid followed by applying Photo Bond and Photo Clearfil Bright Group 9: etched with 10-3 solution followed by applying Photo Bond and Photo Clearfil Bright All the specimens were stored in $37^{\circ}C$ under 50% relative humidity for 24 hours before observations. The specimens in 7, 8, and 9 group, omitting the group 1 to 6, were demineralized in 10% HCl for 10s in order to observe the resin tags. All the specimens in each group were then dried at room temperature. The dried specimens were ion coated with Eiko ion coater (Eiko-engineering Co.), and observed in Hitachi S-430 Scanning electron microscope (Hitachi, Co. Tokyo) at 15KV. The following results were obtained as follows; 1. The smear layers were still remained in group 1,2,4,5, and 6. 2. There is no effect of 5% NaOCl and 30% phosphoric acid on the changes of dentin morphology 3. The dentin treated with 10-3 solution, indicating the tubules opened when the smear layer and the dental plug dissolved. 4. In case of applying the bonding agents the resin tag was not formed at the deep area of dentinal tubules, but in case of applying the Dentin Adhesit$^{(R)}$ that was not.

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Thermoelectric Properties of the n-type Bi2(Te0.9Se0.1)3 Processed by Hot Pressing with Dispersion of 0.5 vol% TiO2 Nanopowders (0.5 vol% TiO2 나노분말을 분산시킨 n형 Bi2(Te0.9Se0.1)3 가압소결체의 열전특성)

  • Park, D.H.;Oh, T.S.
    • Journal of the Microelectronics and Packaging Society
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    • v.20 no.1
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    • pp.15-19
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    • 2013
  • The n-type $Bi_2(Te_{0.9}Se_{0.1})_3$ powders, which were fabricated by melting/grinding method and dispersed with 0.5 vol% $TiO_2$ nanopowders, were hot-pressed in order to investigate the effects of $TiO_2$ dispersion on the thermoelectric properties of the hot-pressed $Bi_2(Te_{0.9}Se_{0.1})_3$. Excellent thermoelectric properties such as a maximum figure-of-merit of $2.93{\times}10^{-3}/K$ and a maximum dimensionless figure-of-merit of 1.02 were obtained for the hot-pressed $Bi_2(Te_{0.9}Se_{0.1})_3$. With dispersion of 0.5 vol% $TiO_2$ nanopowders, the maximum figure-of-merit and the maximum dimensionless figure-of-merit decreased to $2.09{\times}10^{-3}/K$ and 0.68, respectively.

Fabrication and Characterization of the Carbon Fiber Composite Sheets (탄소섬유를 이용한 열가소성 복합재료 시트 제조 및 특성)

  • Lee, Yun-Seon;Song, Seung-A;Kim, Wan Jin;Kim, Seong-Su;Jung, Yong-Sik
    • Composites Research
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    • v.28 no.4
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    • pp.168-175
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    • 2015
  • Recently, the applications of carbon fiber reinforced plastics (CFRPs) have become broader than ever when it comes to such industries as automotive, ships, aerospace and military because of their lightweight-ness and high mechanical properties. Thermosetting plastics like epoxy are frequently used as the binding matrix in CFRPs due to their high hardness, wetting characteristics and low viscosity. However, they cannot melted and remolded. For this reason, thermosetting plastic wastes have caused serious environmental problems with the production of fiber reinforced plastics. Thus, many studies have focused on the carbon fiber reinforced thermoplastics (CFRTPs) and recycling carbon fiber. In this study, recycled carbon fiber (RCF) was prepared from CFRPs using a pyrolysis method, which was employed to separate resin and carbon fiber. The degree of decomposition for epoxy resin was confirmed from thermal gravimetric analysis (TGA) and scanning electron microscope (SEM). The RCF was cut and ground to prepare a carbon fiber composite sheet (CFCS). CFCS was manufactured by applying recycled carbon fibers and various thermoplastic fibers. Various characterizations were performed, including morphological analyses of surface and cross-section, mechanical properties, and crystallization enthalpy of CFCS at different cooling conditions.