• 동아시아식생활학회지
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• 제20권1호
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• pp.46-53
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• 2010

The study examined the effects of dietary green tea powder supplementation on blood glucose, and plasma and liver lipid concentrations in diabetic rats. Twenty-five male Sprague-Dawley rats (body weight $200{\pm}5\;g$) were divided into two groups (diabetic and non-diabetic), which were each randomly divided into two subgroups that were fed a control and 1% green tea powder-supplemented diet. Serum and liver lipid concentrations were measured by established techniques. Low density lipoprotein-cholesterol (LDL-C) was calculated from an established equation. Body weight gain and feed efficiency ratio were lower in diabetic rats than in non-diabetic rats regardless of diet. There were no differences in weight gain in diabetic and non-diabetic rats consuming the control and green tea powder-supplemented diets. The levels of fasting plasma glucose, serum total cholesterol, triglyceride, LDL-C and atherogenic index of diabetic rats were significantly higher than that of non-diabetic rats. Conversely, the levels of high density lipoprotein-cholesterol (HDL-C) of diabetic rats was significantly lower than that of non-diabetic rats. Fasting plasma glucose, serum total cholesterol, triglyceride, LDL-C and atherogenic index were significantly lower in diabetic rats fed the green tea powder diet than in rats fed the control diet, and HDL-C was significantly higher in rats fed the green tea powder diet than in rats fed the control diet. The content of liver total cholesterol and triglyceride of diabetic rats were significantly higher than that of non-diabetic rats. Liver total cholesterol and triglyceride were significantly lower in diabetic rats fed green tea powder-supplemented diet than in rats fed the control diet. It is concluded that green tea powder supplementation positively influences blood glucose and lipid metabolism in diabetic rats. The present study, although not directly applicable to humans, may have some implications for individuals who habitually consume green tea powder.

• 한국식품영양학회지
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• 제13권1호
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• pp.36-44
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• 2000

Survival of pathogenic bacteris(S. aureus, L. monocytogenes, E. coli and S. typhimurium) in tryptic soy broth containing green tea water extract(GTW), green tea ethanol extract(GTE), potassium sorbate (PS) and sodium benzoate(SB) stored at various pH was evaluated. Tryptic soy broth(TSB) containing 0∼2%(w/v) of green tea extracts and preservatives adjusted to pH 5.5, 6.0, 6.5 and 7.0 was inoculated approximately 105 CFU/ml of pathogenic bacteria and incubated at 35$^{\circ}C$ for 24∼48 hours. Survival of bacteria was determined by viable cell counts of bacterial culture at each pH. Minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of green tea extracts and preservatives against pathogenic bacteria were derived from survival curves of each bacteria. Antibacterial activities of green tea extracts increased with increasing pH but those of preservatives decreased with increrasing pH. S. aureus was the most sensitive strain to GTW and GTE but the most resistant to PS and SB. The MICs of green tea extracts to S. aureus were 0.52∼0.98% at pH 5.5∼6.0 and non inhibitory at pH 7.0. S. typhimurium was the most resistant to green tea extracts while the most sensitive to SB. The MICs of green tea extracts to S. typhimurium were 0.46∼1.62% at pH 5.5∼6.0 and 2% of PS was bactericidal at pH 5.5. 1.0∼2.0% of GTE were bactericidal to all strains tested except L. m9oncytogenes at pH 7.0. GTE was most efficient at inactivating pathogenic bacteria, generally followed by GTW, PS and SB.

• 한국식품조리과학회지
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• 제15권3호
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• pp.224-230
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• 1999

The purpose of this study was to find out the optimal mixing ratio of green tea powder, sugar, and water for the preparation of Seolgiddeok through Response Surface Methodology based on the color, sensory, and texture test. The oganoleptic and textural properties of Seolgiddeok prepared with various concentrations of green tea powder (0% (control group), 0.5% (GT-0.5 group), 1% (GT-1.0 group), 1.5% (GT-1.5 group), 2% (GT-2.0 group)), and their quality changes during storage were also investigated. The optimal mixing ratio of green tea powder, sugar, and water for preparing the best quality Seolgiddeok was 1.0%, 12%, and 22%, respectively. The proximate composition of green tea powder was 21.70% of crude protein, 8.49% of crude lipid, 2.95% of reducing sugar, and 6.40% of ash. The contents of crude lipid, reducing sugar, and catechins in Seolgiddeok added with a green tea powder were increased with increasing the content of green tea powder. The hardness and gumminess of GT-1.0 group were the lowest among four groups, whereas GT-1.0 and GT-2.0 groups had the lowest cohesiveness. While the control group was the lowest in adhesiveness, the springness was not significantly different among all groups. GT-0.5 and GT-1.5 groups were the highest in sweet taste and colorfulness, respectively. However, GT-1.0 group had the best overall quality. Total microbial numbers, the acidity and pH in Seolgiddeok during storage were decreased with increasing green tea powder content, and especially those of GT-1.0 and GT-1.5 groups were relatively the lower than others. The “L” value (lightness) of the control group (no additives) was the highest among five groups, and the value was decreased with storage period, and especially GT-0.5 groups had the lowest brightness. The“a”value (reddness) of the control group was the highest, followed by GT-0.5, GT-1.0, GT-1.5, and GT- 2.0 group in order. The“b”value (yellowness) was increased with the increase of green tea powder content. Above results indicated that GT-1.0 group showed the best quality of Seolgiddeok through organoleptic and rheology tests.

• 한국식품조리과학회지
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• 제15권4호
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• pp.395-400
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• 1999

가루녹차의 식빵 적성을 검토하기 위하여 가루녹차를 0, 2.5, 5.0, 그리고 10%를 첨가한 식빵을 제조하여 품질의 특성을 조사하였다. 아밀로그램의 특성을 보면 가루녹차의 첨가량이 증가할수록 최고점도와 최종점도는 감소하였으나 호화개시온도는 증가하는 경향이었다. 식빵 외부의 L값, a값 및 b값은 가루녹차 함량이 증가할수록 감소하였고, 내부의 색도도 L값과 a값은 가루녹차 첨가량이 많을수록 감소되었고 b값 증가하였다. 식빵의 부피는 가루녹차 5.0%이상 첨가시에 감소하였다. 식빵의 물리적 특성을 보면 견고성 (hardnesss)은 대조군과 2.5% 첨가군에서는 차이가 없었으나 10% 첨가군에서는 유의적으로 높았고, 탄력성(springiness)과 응집성(cohesiveness)은 가루녹차 첨가에 의한 영향이 거의 없었고 전체적으로는 가루녹차 10% 첨가 군에서 높은 값을 나타내었다. 관능검사 결과, 외관, 색, 풍미, 맛, 조직감, 그리고 전반적인 기호도 모두 가루녹차 2.5% 첨가한 식빵이 좋다고 평가되었다. 이상의 결과를 종합해 보면 가루녹차 식빵은 기호도와 전반적인 특성을 볼 때 가루녹차 2.5% 첨가가 가장 우수하였으며 부피 등의 감소에 대하여는 앞으로 더 연구해야 할 과제라고 생각한다.

• 한국임상약학회지
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• 제28권4호
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• pp.342-346
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• 2018

Background: Green tea extracts are approved as nonprescription drug and available as health functional foods, health foods, and beverages. Clinical information on the products is lacking. Methods: Information about the products on green tea nonprescription drugs was obtained from the website of the Korea Pharmaceutical Information Center. The Naver, i.e., a top ranking online search portal, was used for compiling the list of the health functional food products using key words of 'green tea catechin' on August 23, 2018. The recommended daily dosages of catechins were calculated as 30% of the total dried mass of green tea and about 50% of the catechins were considered as epigallocatechin gallate (EGCG). Results: A total of two types of nonprescription drugs containing green tea powder or extracts, nine health functional food products, and three types of health foods were found. The regulatory requirements of the EGCG exceeding 800 mg were reported to be associated with adverse effects of elevated liver enzyme. If consumers take several green tea products concurrently, such as nonprescription drugs with health functional foods or health foods, it could exceed the recommended amount of EGCG. Conclusion: The concurrent use of green tea products as nonprescription drugs, health functional foods, and healthy foods may lead to an increased exposure to EGCG. Pharmacists should be aware the availability of various types of green tea products and the potential risk of liver toxicity due to excessive consumption of EGCG.

• 한국식품위생안전성학회지
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• 제13권4호
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• pp.377-382
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• 1998

항산화효과, 항돌연변이효과, 콜레스테롤농도 상승억제작용이 우수한 것으로 알려진 차추출물의 주요성분을 분리 정량하기 위하여 각 발효정도에 따른 분류 및 녹차의 채엽시기별에 따라 녹차 1번차 녹차2번차 녹차 3번차, 우롱차 및 홍차 등에 대한 일반성분, 아미노산, Vit.C, Vit. E, ${\beta}-carotene$, 카테킨류, 카페인, 데오브로마인 및 데오필린 등을 분석하고 특히 항산화성분인 카테킨류의 항산화효과 및 항돌연변이 효과의 연구에 기초자료로 이용하고자 하였다. 녹차 I, II, III, 우롱차 및 홍차의 일반 성분중 조지방함량은 각각 1.1, 2.5, 4.9, 0.8 및 1.2% 이었으며, 총 아미노산함량은 녹차I>녹차II>녹차III의 순이었고 대부분의 아미노산은 차 성분의 유일한 아미노산인 theanine이 차지하였다. 녹차 I, II, III, 우롱차 및 홍차에 함유된 Vit. C함량은 각각 101.6, 87.5, 95.9, 99.1 및 108.0 mg%이었으며, ${\beta}-carotene$ 함량은 각각 270, 268, 481, 80 및 181 ppm이었고, Vit. E 함량은 ${\alpha}-tocopherol$이 대부분을 차지하였으며 ${\beta}-$ 및 ${\delta}-tocopherol$은 모두 검출되지 않았다. 한편, 총카테킨류의 함량은 각각 10.5, 10.4, 7.2, 8.4 및 1.8%로 총카테킨류중 EGCG의 함량이 가장 높았다. 카테킨류 중 EGC의 함량은 녹차I>녹차III>녹차II>우롱차>홍차의 순으로 녹차I이 가장 높았으며, EGCG와 ECG의 함량은 우롱차>녹차I>녹차II>녹차III>홍차의 순으로 우롱차가 가장 높았다. 또한 GA함량은 0.01, 0.02, 0.05, 0.13 및 0.31%로 홍차에 가장 많은 것으로 나타났으며, 카페인 및 데오브로마인 함량도 홍차의 경우가 가장 높았으나 데오필린 함량은 녹차I이 가장 높게 나타났다.

• 생명과학회지
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• 제20권9호
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• pp.1415-1419
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• 2010

최근 웰빙 열풍으로 나날이 녹차에 대한 관심과 소비가 증가하고, 생산 재배되고 있는 산지에 대한 브랜드화가 진행되고 있다. 하지만 지역에서 재배되고 있는 녹차품종의 구별 및 차이에 대해서는 아직 많은 연구가 되어 있지 않고 있다. 이 연구에서 국내 대표 녹차산지인 하동지역과 보성지역에서 채집한 녹차와 중국과 일본의 대표적 녹차품종을 가지고 STS-RFLP분석을 수행하였다. 페닐프로파노이드 생합성 경로에 관여하는 페닐알라닌 암모니아 리아제와 찰콘 합성효소 그리고 디하이드로플라보놀 4-리덕타아제 유전자의 암호영역과 비암호영역을 사용하여 이들 품종들을 구별할 수 있는 연구에 성공하였다. 이 논문에서는 녹차품종 구별에 사용 가능한 STS-RFLP법과 프라이머를 나타내었고, 하동지역과 보성지역의 녹차품종을 CHS 유전자의 CAPS 마커만으로 구별할 수 있는 방법을 찾아내어, 국내 지역간 품종의 구분 및 검증에 사용할 수 있다는 사실을 제시하였다.

• 한국식품영양과학회지
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• 제23권2호
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• pp.287-292
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• 1994

This paper was investigated to elucidate the nitrite-scavenging action as one of biological actions of green tea extracts. Green tea extracts exhibited remarkable nitrite-scavenging action. Among the green tea extracts, steamed green tea showed higher nitrite-scavenging action than roasted green tea. Nitrite-savenging actions of gren tea extracts were hardly influenced until three times extraction with hot water. Furthermore , the nitirte-scavenging action of green tea extracts showed ph dependent , highest at pH 1.2 and lowest at pH 6.0. The nitrite -scavenging rate in sample extracts /amine /nitrite systems exhibited faster than that in amine/nitrite systems. In order to screen the nitirte-scavenging factors, samples extracts were fractionated with methanol. in both samples, methanol-soluble fractions possessed greater nitrite-scavenging action than methanol-insoluble fractions .The nitrite-scavenging action of methanol-soluble fractions increased with proportion to absorbance at 420nm and at 280nm, while that of green tea extracts increased with the contents of total phenols and absorbance at 280nm.

• 한국유기농업학회지
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• 제19권spc호
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• pp.17-20
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• 2011

Plant biomass is a huge carbon-complex that has potential as a nutrient. Therefore we extracted and separated useful materials for plant growth from tea leaf and stem. The pre-treatment process including high temperature ($200^{\circ}C$) and pressure (20-40 kgf/$cm^2$) was treated for several minutes and extracted at $120^{\circ}C$ for 30-60 minutes. After that the chemical compositions and ingredients were analyzed from that plant-nutrient. As a result of mineral contents, calcium and magnesium concentrations are higher than other minerals. Also the result of carbohydrates analyses has shown that the sugar oligomer consists of xylose(95.3%) and glucose(4.7%), and the sugar monomer consists in the order of xylose (52.7%) > manose (22.8%) > arabinose (10.8%) > galactose (10.2%) > glucose (3.5%). Before applied to field, in vitro plant growth system and formulation were examined. To evaluate the effect of the nutrients, both strawberry green-house and persimmon fields were used in this test. The treated persimmons were heavier than controls scored at 13-22%. In addition, the storage-period was extended in the treated strawberries. Interestingly in the treated strawberry, the contents of polyphenols were increased (38-57%). These results suggest that the plant-nutrient can afford to help for plant growth and storage, and it can be substituted for other commercial nutrients. In conclusion, this plant-nutrient may help to extend eco-friendly or organic farming in Hadong-gun area.

• 한국식품영양과학회지
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• 제31권5호
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• pp.856-861
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• 2002

본 연구는 녹차의 섭취기간에 따른 질병 발현 정도를 조사하기 위하여 경상남도 하동군에 거주하는 주민 92명을 대상으로 설문조사를 통하여 이루어졌다. 조사대상자들의 녹차 섭취기간에 따라 녹차를 마시지 않는 대조군과 10년 미만 녹차 섭취군 및 10년 이상 녹차 섭취군의 3분위로 나누어 녹차의 장기간 섭취가 건강관련 변인과 식습관에 미치는 영향을 분석하였다. 그 결과는 다음과 같다. 조사대상자의 일반적 특성은 전체 대상자 중 남자가 41명, 여자가 51명이었으며, 평균연령은 대조군이 62.0세, 2분위 60.1세, 3분위 57.2세로 나타났다. 건강관련 생활습관으로 음주여부는 1분위에서 45.5%, 2분위 36.1%, 3분위 37.5%가 음주를 하는 것으로 나타나, 녹차를 마시는 집단에서의 음주비율이 낮게 나타났다. 마시는 술의 종류로는 소주가 가장 많은 비중을 차지하였으며, 음주의 빈도는 1, 3분위에서 주 5～6회 마신다가 50%이상, 2분위는 주 1～2회가 53.8%로 가장 많이 차지하였다. 흡연여부는 80%정도가 거의 담배를 피우지 않는 것으로 조사되어 바람직한 흡연행동을 나타내었다. 신체활동정도는 모든 집단이 농사일에 직접 참여함으로써 왕성한 활동정도를 보였으며, 건강보조식품은 잘 섭취하지 않는 것으로 조사되었다. 질병유병률을 조사한 결과, 충치는 1분위 9%, 2분위 27%, 3분위 36%가 충치가 없다고하여 녹차의 장기간 섭취가 충치 예방에 다소간의 영향을 미쳤음을 알 수 있었다. 감기는 각 분위에서 77%, 87%, 76%로 감기를 잘 앓지 않는 것으로 조사되었으나 유의성은 없었다. 골다공증에 관한 문진 결과 29%, 64%, 100%순으로 정상이라고 응답하였다. 그러나 골다공증 검사율이 저조하여 녹차의 효과를 비교하기는 힘들었다. 식습관 점수는 녹차를 장기간 섭취할수록 유의적으로 좋은 점수를 나타내었다. 본 연구의 대상자들은 녹차를 접할 기회가 많고 주로 질병의 예방 및 개선을 목적으로 마시고 있었기에 녹차의 기능성에 대해서 많은 관심과 기대를 보여주고 있었다. 게다가 본 조사의 결과, 녹차의 장기간 음용이 충치, 골다공증과 같은 질병의 예방 및 식습관 향상에 영향을 미침을 알 수 있었고 나아가 녹차의 섭취를 증가시키기 위해서는 차 이외의 다양한 음식과 생활용품으로의 개발이 필요함을 알 수 있었다.