• Ecology and Resilient Infrastructure
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• 제7권3호
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• pp.199-207
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• 2020

전 세계적으로 홍수, 가뭄, 열파 등 기상재해가 빈발하는 환경에서 도시공원은 휴식, 문화, 생태적 기능뿐만 아니라 도시민의 안전을 위한 기능도 제공해야 한다. 본 연구는 도시 내 기후변화에 대응하는 녹색 공간인 생활권 공원에 복합기능을 확보하는 목적으로 도시인의 안전을 위한 도시방재공원의 분류체계를 제안하였다. 문헌 조사를 통해 분석지표를 추출하고, 대상지 현장조사 및 관련자 인터뷰를 통해 분류체계를 검증하였다. 평가를 위한 대분류는 도시공원의 입지, 공간구성, 방재복합시설 3가지로 구분하고, 실증 분석을 통하여 도출된 문제점에 대한 개선방향을 제안하였다.

• 한국안전학회지
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• 제39권1호
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• pp.27-32
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• 2024

This study analyzed cases of hydrogen (H₂) and natural gas (CH₄) leakage from a hydrogen-blended natural gas pipeline to determine a range of leakage characteristics, including leakage type, pipe material, pipe diameter, pressure, and damage size. Based on the results of this analysis, five hydrogen-blended natural gas leakage scenarios were selected. The national vision for a carbon-neutral society by 2050 is a very important strategic objective and promotes environmentally sustainable economic development in the age of the climate crisis. Accordingly, zero-carbon and low-carbon policies are being promoted in various fields, including energy production, consumption, and industrial processes. Hydrogen-blended natural gas is eco-friendly and is considered an important step towards carbon neutrality, with various countries including the United States and several European countries conducting empirical research to further investigate its potential. In Korea, a national research project commenced in April 2023 to verify and demonstrate the life cycle safety of blending hydrogen into the natural gas network. The results of this study will provide important data for the analysis of the damage impacts caused by the leakage of hydrogen-blended natural gas, such as the diffusion of gas clouds, fires, and gas explosions.

• Korean Chemical Engineering Research
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• 제53권3호
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• pp.327-332
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• 2015

A large amount of dye wastewater poses a threat to environmental safety. Disperse blue, an anthraquinone dye that is widely used in textile dyes, is difficult to degrade in wastewater. In this work, one fungus was screened according to the decolorization rate of disperse blue. The fungus was identified and named Aspergillus XJ-2 on the basis of its morphological characteristics and 18s rDNA. Response surface method was used to optimize culture conditions for A. XJ-2. The optimum values of obtained responses were as follows: temperature, $35^{\circ}C$; pH, 5.2; carbon-to-nitrogen ratio, 30:5.5; and rotation ratio, $175r{\cdot}min^{-1}$. Under optimized conditions, the decolorization rate of A. XJ-2 was up to 94.8% in 48 h.

• 한국지역사회생활과학회지
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• 제22권3호
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• pp.327-339
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• 2011

This study aims to investigate relationships between benefit sought and selection attributes of green tea consumers. For data collection, a total of 595 copies of questionnaires were collected by convenience sampling in the Seoul and Gyeonggi-do area. The data were analyzed by using SPSS 15.0. The factor analysis identified four dimensions of the benefit sought : health benefit, sensory, sociality, and self-esteem. Six dimensions of selection attributes were identified as manufacturing, design, sensory appeal, recommendation, utility and brand. The results of the canonical correlation analysis indicated that health benefit, sensory, sociality of benefit sought and manufacturing, design, sensory appeal, recommendation, utility, brand of selection attributes were highly correlated, and the self-esteem of benefit sought and recommendation of selection attribute were highly correlated. This means it is important to place an emphasis on safety production, package design, sensory characteristics, product description, utility and brand for consumers who seek health benefit, flavor and sociality. It is also important to place an emphasis on product description for consumers who pursue self-esteem benefits. Green tea marketers should consider benefit sought aspects as the most important factors affecting selection attributes on green tea purchasing.

• 한국환경보건학회지
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• 제41권5호
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• pp.358-367
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• 2015

Objectives: This study was performed to evaluate the analytical method for PAH metabolites in human urine using enzyme hydrolysis and solid-phase extraction coupled with LC-(ESI)-MS/MS technique. Methods: We employed HPLC tandem mass spectrometry techniques with appropriate pre-treatment for analysis of 16 OH-PAHs in human urine. Samples were hydrolysis by ${\beta}$-flucuronidase/Aryl sulfatase, and target compounds were extracted by solid-phase extraction with a strata-x cartridge. Cross-validation was performed between Eulji University and Green Cross laboratories with 200 human urine samples. Results: The accuracies were between 90.3% and 118.8%, and precisions (relative standard deviations) were lower than 10%. The linearity obtained was satisfying for the 16 OH-PAH compounds, with a coefficient of determination ($r^2$) higher than 0.99. The results of cross-validation at the two organizations were compared by ICC (interclass correlation coefficient) values. The cross-validation results were excellent or good for all compounds. Conclusion: An analytical method was validated for low nanogram levels of 16 OH-PAHs in human urine. Also, satisfying results were obtained for method validation such as accuracy, precision and ICC of cross-validation.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.19-25
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• 2006

With particular regards to the hepatitis A virus (HAV), a terminal dry-heat treatment ($100^{\circ}C$ for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor IX concentrate. The loss of factor IX activity during dry-heat treatment was of about 3%, as estimated by a clotting assay. No substantial changes were observed in the physical and biochemical characteristics of the dry-heat-treated factor IX compared with those of the factor IX before dry-heat treatment. The dry-heat-treated factor IX was stable for up to 24 months at $4^{\circ}C$, The dry-heat treatment after lyophilization was an effective process for inactivating viruses. The HAV and murine encephalomyocarditis virus (EMCV) were completely inactivated to below detectable levels within 10 min of the dry-heat treatment. Porcine parvovirus (PPV) and bovine herpes virus (BHV) were potentially sensitive to the treatment. The log reduction factors achieved during lyophilization and dry-heat treatment were ${\ge}5.60$ for HAV, ${\ge}6.08$ for EMCV, 2.64 for PPV, and 3.59 for BHV. These results indicate that dry-heat treatment improves the virus safety of factor IX concentrates, without destroying the activity. Moreover, the treatment represents an effective measure for the inactivation of non-lipid enveloped viruses, in particular HAV, which is resistant to solvent/detergent treatment.

• 한국식품위생안전성학회지
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• 제13권4호
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• pp.377-382
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• 1998

항산화효과, 항돌연변이효과, 콜레스테롤농도 상승억제작용이 우수한 것으로 알려진 차추출물의 주요성분을 분리 정량하기 위하여 각 발효정도에 따른 분류 및 녹차의 채엽시기별에 따라 녹차 1번차 녹차2번차 녹차 3번차, 우롱차 및 홍차 등에 대한 일반성분, 아미노산, Vit.C, Vit. E, ${\beta}-carotene$, 카테킨류, 카페인, 데오브로마인 및 데오필린 등을 분석하고 특히 항산화성분인 카테킨류의 항산화효과 및 항돌연변이 효과의 연구에 기초자료로 이용하고자 하였다. 녹차 I, II, III, 우롱차 및 홍차의 일반 성분중 조지방함량은 각각 1.1, 2.5, 4.9, 0.8 및 1.2% 이었으며, 총 아미노산함량은 녹차I>녹차II>녹차III의 순이었고 대부분의 아미노산은 차 성분의 유일한 아미노산인 theanine이 차지하였다. 녹차 I, II, III, 우롱차 및 홍차에 함유된 Vit. C함량은 각각 101.6, 87.5, 95.9, 99.1 및 108.0 mg%이었으며, ${\beta}-carotene$ 함량은 각각 270, 268, 481, 80 및 181 ppm이었고, Vit. E 함량은 ${\alpha}-tocopherol$이 대부분을 차지하였으며 ${\beta}-$ 및 ${\delta}-tocopherol$은 모두 검출되지 않았다. 한편, 총카테킨류의 함량은 각각 10.5, 10.4, 7.2, 8.4 및 1.8%로 총카테킨류중 EGCG의 함량이 가장 높았다. 카테킨류 중 EGC의 함량은 녹차I>녹차III>녹차II>우롱차>홍차의 순으로 녹차I이 가장 높았으며, EGCG와 ECG의 함량은 우롱차>녹차I>녹차II>녹차III>홍차의 순으로 우롱차가 가장 높았다. 또한 GA함량은 0.01, 0.02, 0.05, 0.13 및 0.31%로 홍차에 가장 많은 것으로 나타났으며, 카페인 및 데오브로마인 함량도 홍차의 경우가 가장 높았으나 데오필린 함량은 녹차I이 가장 높게 나타났다.

• 한국작물학회지
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• 제60권1호
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• pp.91-96
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• 2015

본 연구에서는 최근 12년간의 1월 평균최저기온과 배수 등급을 기준으로 남한지역 논토양에서 녹비작물 재배적지를 재설정한 결과는 다음과 같다. 1. 헤어리베치와 트리티케일의 재배가능지대 온도는 $-10^{\circ}C$ 이상으로 논면적은 935,993 ha이었으며, 재배안전지대는 $-8^{\circ}C$ 이상으로 논면적은 836,214 ha였으며, 이는 전체 논 면적 966,076 ha(통계면적)의 각각 96.9 및 86.6%에 해당되었다. 2. 녹비보리의 재배가능지대 온도는 $-8^{\circ}C$이상이며, 재배 안전지대 온도는 $-6^{\circ}C$이상이며, 해당 재배면적은 각각 836,421 ha (전체 논 면적의 86.58%) 및 513,592 ha(전체 논 면적의 53.1%)였다. 3. 호밀의 재배가능 및 재배안전지대 온도는 $-20^{\circ}C$ 이상으로 거의 대부분의 지역이 해당되었으며, 그 면적은 963,253 ha로 전체 논 면적의 99.7%에 달했다 4. 클로버의 재배가능지대 온도는 $-6^{\circ}C$이상이었으며, 재배 안전지대 온도는 $-4^{\circ}C$이상으로 해당 재배면적은 512,592 ha와 152,299 ha로 전체 논 면적의 37.3%와 15.8%로 가장 적었다.

• 한국지반신소재학회논문집
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• 제5권3호
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• pp.9-15
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• 2006

그린월 공법은 독립식 콘크리트 보형식의 옹벽으로, 전, 후면 가로보와 세로 방향으로 버팀보를 연속적으로 쌓아 올려 내부에 다짐성토를 시행함으로써 구조체가 일체 거동하도록 강성벽체(rigid body)를 형성시키는 옹벽 구조물이다. 최근의 그린월공사는 원지반 절취량을 최소화 할 수 있다는 장점으로 절토 공사현장에 다양하게 적용되고 있으며, 주로 앵커 및 쏘일네일 등과 함께 시공하는 추세이다. 이처럼 그린월과 함께 쏘일네일이 시공되는 경우에는 전면벽체의 강성이 크기 때문에 연성전면벽체의 경우 보다 전체 시스템의 안정성 증대가 예상되나 실제 설계에서는 이를 반영하지 않고 쏘일네일만 설계하므로 비경제적인 요소가 많은 실정이다. 따라서 본 연구에서는 그린월과 같은 강성전면벽체와 쏘일네일 공법이 함께 시공되는 경우에 대하여 강성전면벽체로 인한 전체 시스템의 안정성 증대효과를 평가해 보았다. 본 연구에서는 전면벽체의 강성변화에 따른 전체 시스템의 안정성 증대효과를 평가하기 위하여 실내모형실험을 수행하였으며, 한계평형해석을 시행하여 그린월과 같은 전면벽체의 강성이 전체 활동에 대한 안전율에 미치는 영향을 평가하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권6호
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• pp.340-346
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• 2002

The purpose of the present study was to examine the efficacy and mechanism of the PAB (para-amino benzamidine) affinity column chromatography, Viresolve NFP virus filtration, pasteurization (60$\^{C}$ heat treatment for 10 h), and lyophilization steps employed in the manufacture of urokinase from human urine as regards the removal and/or inactivation of the hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and subjected to scale-down processes mimicking the manufacture of urokinase Samples were collected at each step, immediately titrated using a 50% tissue culture infectious dose (TCID$\_$50/), and the virus reduction factors evaluated. PAB chromatography was found to be an effective step for removing HAV with a log reduction factor of 3.24. HAV infectivity was rarely detected in the urokinase fraction, while most of the HAV infectivity was recovered in the unbound and wash fractions. HAV was completely removed during the Viresolve NFP filtration with a log reduction factor of $\geq$ 4.60. Pasteurization was also found to be an effective step in inactivating HAV where the titers were reduced from an initial titer of 7.18 log$\_$10/ TCID$\_$50/ to undetectable levels within 10 h of treatment. The log reduction factor achieved during pasteurization was $\geq$ 4.76. Lyophilization revealed the lowest efficacy for inactivating HAV with a log reduction factor of 1.48. The cumulative log reduction factor was $\geq$ 14.08. Accordingly, these results indicate that the production process for urokinase exhibited a sufficient HAV reducing capacity to achieve a high margin of virus safety.