• 한국유기농업학회지
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• 제13권2호
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• pp.161-173
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• 2005

This study was carried out to clarify the effects of antifungal bacterial strains isolated from the greenhouse soil grown strawberry plants on the growth inhibition of plant pathogen, gray mold (Botrytis cinerea) infected in strawberry plants in Damyang and Jangheung districts. Antagonistic bacterial strains were isolated and investigated into the antagonistic activity against gray mold. Screened ten bacterial strains which strongly inhibited Botrytis cinerea were isolated from the greenhouse grown strawberry plants, and the best antifungal microorganism designated as SB 143 was finally selected. Antifungal bacterial strain SB 143 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. SB 143 showed 59.4% of antifungal activity against Botrytis cinerea. By the bacterialization of culture broth and heated filtrates of culture broth, Bacillus sp. SB 143 showed 93.1% and 32.1% of antagonistic activity against Botrytis cinerea.

• 식물병연구
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• 제29권3호
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• pp.295-298
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• 2023

During a disease survey conducted in April 2022, we observed severe gray mold symptoms on inflorescences of mango trees (Mangifera indica) grown in a vinyl greenhouse in Jeju, Korea. The symptoms occurred on the flowers and peduncles, on which a lot of gray molds were formed. The incidence of gray mold on the inflorescences in the vinyl greenhouse ranged from 10% to 40%. Three fungal isolates were obtained from the lesions and identified as Botrytis cinerea based on their morphological characteristics and phylogenetic analysis. All isolates of B. cinerea were tested for their pathogenicity to inflorescences of mango trees through artificial inoculation. The pathogenicity of the isolates was confirmed on the flowers and peduncles. The symptoms induced by the isolates were similar to those on the inflorescences of mango trees observed in the disease survey. This is the first report of B. cinerea causing gray mold in mango in Korea.

• 한국균학회지
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• 제51권4호
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• pp.259-263
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• 2023

During crop disease surveys in 2019 and 2021, we observed gray mold symptoms on the leaves of wasabi (Eutrema japonicum) plants grown in vinyl greenhouses in Taebaek and Pyeongchang, Gangwon Province, Korea. The symptoms appeared at the edges of the leaves, where the infected areas turned black and rotted, and many gray molds formed on the lesions. The incidence of gray mold on the leaves in the vinyl greenhouses at the two locations ranged from 1 to 30%. Four fungal isolates were obtained from leaf lesions and identified as Botrytis cinerea based on morphological characteristics and phylogenetic analysis. Two isolates of B. cinerea were used for pathogenicity tests on the leaves of wasabi plants by artificial inoculation. The pathogenicity of the isolates was confirmed in leaves. The symptoms induced by the isolates were similar to those observed in wasabi leaves in the vinyl greenhouses investigated. This is the first report of B. cinerea causing gray mold in wasabi in Korea.

• 식물병과 농업
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• 제5권1호
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• pp.55-57
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• 1999

Botrytis cinerea was isolated from a gray mo이 leaf lesion on persimmon in fields of Kyeonsangnam-do from 1996 to 1998, and etiological study was conducted including physiological characteristics. It formed gray mold lesion with light green color on leaves of persimmon (Diospyros kaki). The temperature range for mycelial growth was between $5^{\circ}C$ and $30^{\circ}C$ with the optimum temperatures of $20^{\circ}C$ to $25^{\circ}C$. Conidia were oviod, cylindric, and colorless and their dimensions in culture were $8.4~11.5\times7.0~8.9\mu\textrm{m}$. The optimum temperature of conidial germination was $25^{\circ}C$. Sclerotia on potato dextrose agar medium were well formed and brownish condiophores were observed with their size of $18.5~64.9\times4.5~8.0\mu\textrm{m}$. Symptoms on artificially inoculated plants were similar to those of gray mold disease on persimmon caused by Botrytis cinerea in fields.

• 한국유기농업학회지
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• 제14권4호
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• pp.399-410
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• 2006

전남 남부지역의 참다래 과수원에서 발생하는 잿빛곰팡이 병원균(gray mold, Botrytis cinerea)에 대한 항균작용이 우수한 세균성 균주를 선발하기 위하여 참다래 과수원 토양으로부터 단일균주를 분리하였으며, 잿빛곰팡이 병원균(Botrytis cinerea)에 대한 생물적 제어 능력을 검정하고 균주 동정을 실시하였다. 참다래 과수원에서 분리한 총 350여종의 단일균 주 중에서 참다래에서 발생하는 잿빛곰팡이 병원균에 대하여 길항작용이 우수한 균주를 1차적으로 4종 선발하였고, 이 중에서 참다래 잿빛곰팡이 병원균에 대하여 길항작용이 86.9% 정도로 우수한 CHO 163을 최종적으로 선발하였다. 참다래 잿빛곰팡이 병원균에 대하여 길항작용이 우수한 CHO 163을 대상으로 균주의 형태적 성질, 배양적 특성 및 생리 생화학적 성질 등을 조사하여 균주의 동정을 검토한 바 Bacillus sp.와 유사한 균주로 동정되었다. 길항균으로 분리한 Bacillus sp. CHO 163은 한천배지에서 참다래 잿빛곰팡이 병원균 접종 후 길항균 처리와 열처리한 균주 배양액을 처리하였을 때 거의 완전한 항균작용을 보였다. 또한, in vitro 상에서 참다래에 잿빛곰팡이병원균을 접종하고 Bacillus sp. CHO 163의 배양액을 처리한 결과 잿빛곰팡이병원균의 방제가 확인되었다.

• 농업생명과학연구
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• 제50권2호
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• pp.25-32
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• 2016

A severe outbreak of gray mold on kenaf (Hibiscus cannabinus L.) was observed on kenaf grown in the research field of Gyeongsangnam-do Agricultural Research and Extension Services, Jinju, Korea in 2014. Gray mold appeared on young plants as gray-brown velvety mold covering stems and leaves. Infections that girdled the stem caused wilting above the infected area and developed a canker. The casual fungus formed grayish brown colonies on potato dextrose agar. The conidia were one celled, mostly ellipsoid or ovoid in shape, colorless or pale brown in color, and 6-18 × 4-10 ㎛ in size. The conidiophores were 15-32 ㎛ in length. These measurements and taxonomic characteristics were most similar to those of Botrytis. DNA sequencing and phylogenetic analysis of the complete internal transcribed spacer rRNA gene region confirmed that the fungal isolates were indeed Borytis cinerea. Koch's postulates were supported by pathogenicity tests conducted on healthy plants. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea. To the best of our knowledge, this is the first report of a gray mold caused by B. cinerea on kenaf in Korea.

• 식물병연구
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• 제10권4호
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• pp.337-340
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• 2004

2003년 4월 경남농업기술원 유리온실과 농가 육묘장에서 Botrytis cinerea에 의한 백일홍 잿빛곰팡이병이 발생하였다. 병징은 잎과 줄기에 발생하여 병든 부분이 수침상으로 물러지고 썩으면서 병반부위에 회색의 곰팡이가 많이 생기고, 심할 경우 감염된 부위는 결국 말라 죽는다. 분생포자는 무색, 단포자이며 난형 또는 타원형으로 크기는 5${\sim}$16${\times}$4${\sim}$8 ${\mu}m$였고, 분생자경 위에 분생포자가 아주 많이 형성되었다. 분생포자경은 갈색으로 격막이 있고, 폭은 12${\sim}$28 ${\mu}m$였다. 균핵의 모양은 납작하거나 부정형이고 색깔은 검은색이었다. 균사생육과 균핵형성 적온은 $20^{\circ}C$였다. 병반에서 분리한 병원균은 Botrytis cinerea Persoon: Fries에 의한 백일홍 잿빛곰팡이병으로 동정되었다.

• 식물병연구
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• 제17권1호
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• pp.75-77
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• 2011

전북 전주시 화원에서 Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병이 발생하였다. 병징은 꽃, 잎과 잎자루가 수침상으로 물러지고 부패되었다. 다습조건에서 병반위에 잿빛색의 분생포자가 많이 형성하였다. 균총은 PDA 배지에서 회갈색이며 균핵을 형성하였다. 분생포자는 단포자, 난형 또는 타원형으로 무색 또는 옅은 갈색이고, 크기는 $7{\sim}14{\times}5{\sim}9\;{\mu}m$이었다. 바이올렛에 발생한 병징과 병원균의 균학적 특징을 조사한 결과 B. cinerea Persoon:Fries에 의한 바이올렛 잿빛곰팡이병으로 명명할 것을 제안한다.

• 한국균학회지
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• 제39권3호
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• pp.213-216
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• 2011

2007년부터 2010년에 걸쳐 국내 8지역에서 블루베리의 병을 조사한 결과, 청양, 당진, 대전, 제주 지역의 온실에서 재배중인 블루베리의 신초, 잎, 꽃, 열매에서 잿빛곰팡이병 증상이 빈번하게 관찰되었으며, 조사된 다른 지역의 포장에서는 잿빛곰팡이병 증상이 관찰되지 않았다. 조사된 온실내 이 병의 발생율은 1~30%였다. 병반에서는 Botrytis속 균이 분리되었으며, 단포자 분리된 27개 균주의 형태적 및 배양적 특성을 조사한 결과, 모두 Botrytis cinerea로 동정되었다. 병원성 검정을 위하여 선발한 4균주를 4품종의 블루베리잎에 분생포자현탁액으로 인공접종한 결과, 4균주 모두 접종한 블루베리 잎에 자연병반과 유사한 병반을 형성하였다. 국내의 온실재배 블루베리에 발생하는 B. cinerea에 의한 잿빛곰팡이병은 본 연구에 의해 처음으로 보고된다.

• 식물병연구
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• 제22권2호
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• pp.107-110
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• 2016

In October 2014, an occurrence of gray mold was observed on young fruits of sponge gourd (Luffa cylindrica) in Sachunmun, Gangneung, South Korea. Symptoms included abundant mycelia growth with gray conidia on young fruits and finally rotting the fruits. The fungus was isolated from symptomatic fruits and its pathogenicity was confirmed. Based on the morphological features and sequence analysis of ITS-5.8S rDNA, G3PDH, HSP60, and RPB2 genes, the pathogen was identified as Botrytis cinerea Pers. This is the first report of gray mold caused by B. cinerea on L. cylindrica in Korea.