• Title/Summary/Keyword: granule type

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Ultrastructural Study on the Development of the Small Granule-Containing Cells in Superior Cervical Ganglion of Human Fetus (인태아 상경신경절내 소형의 과립함유세포에 관한 전자현미경적 연구)

  • Yoon, Jae-Rhyong;Min, Young-Don;Nam, Kwang-Il
    • Applied Microscopy
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    • v.26 no.3
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    • pp.349-367
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    • 1996
  • The development of small granule-containing cell in the superior cervical ganglion was studied by electron microscopic method in human fetuses ranging from 40 mm to 260 mm crown rump length (10 to 30 weeks of gestational age). At 40 mm fetus, the superior cervical ganglion was composed of clusters of undifferentiated cells, primitive neuroblasts, and unmyelinated nerve fibers together with blood vessels. At 90 mm fetus, the superior cervical ganglion consisted of neuroblasts, satellite cell, small granule-containing cells, and unmyelinated nerve fibers. Two morphological types of the small granule-containing cells in the superior cervical ganglion were first indentified at 90 mm fetus, but were rare. Type I granule-containing cell occurred in solitary and had long processes, whereas type II cells tend to appeared in clusters near the blood capillaries. The granule-containing cells were characterized by the presence of dense-cored vesicles ranging from $150{\sim}300nm$ in diameter in both the cell bodies and processes. Other organelles included abundant mitochondria, rough endoplasmic reticulum, neurotubules, and widely distributed ribosomes. The granule-containing cells had long processes similar to those found in principal ganglionic cells. They could be identified by their content in dense-cored vesicles. The small granule-containing cells increased somewhat in size and number with increase of fetal age. Synaptic contacts were first found on the solitary granule-containing cell at 150 mm fetus. Synaptic contacts between the soma and processes of type I granule-containing cells and preganglionic axon terminals were observed. In addition, synaptic junctions between the processes of granule-containing cells and presumed dendrite of postganglionic neuron were also observed from 150 mm onward. On the basis of these features type I granule-containing cells could be considered as interneurons. The clusters of type II granule-containing cells were located in the interstitial or subcapsular portions of the ganglion, and had short processes which ended in close relation to fenestrated capillaries. Therefore it may be infer that clusters of type II granule-containing cells have an endocrine function.

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Ultrastructural Study on Development of the Superior Cervical Ganglion of Human Fetuses (인태아 상경신경절 발육에 관한 전자현미경적 연구)

  • Kim, Dae-Young
    • The Korean Journal of Pain
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    • v.11 no.1
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    • pp.7-22
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    • 1998
  • The development of the superior cervical ganglion was studied by electron microscopic method in human fetuses ranging from 40 mm to 260 mm of crown-rump length(10 to 30 weeks of gestational age). At 40 mm fetus, the superior cervical ganglion was composed of clusters of undifferentiated cell, primitive neuroblast, primitive supporting cell, and unmyelinated fibers. At 70 mm fetus, the neuroblasts and their processes were ensheated by the bodies or processes of satellite cells. The cytoplasm of the neuroblast contained rough endoplasmic reticulum, mitochondria, Golgi complex, Nissl bodies and dense-cored vesicles. As the neuroblasts grew and differentiated dense-cored vesicles moved away from perikaryal cytoplasm into developing processes. Synaptic contacts between the cholinergic axon and dendrites of postganglionic neuron and a few axosomatic synapses were first observed at 70 mm fetus. At 90 mm fetus the superior cervical ganglion consisted of neuroblasts, satellite cells, granule-containing cells, and unmyelinated nerve fibers. The ganglion cells increased somewhat in numbers and size by 150 mm fetus. Further differentiation resulted in the formation of young ganglion cells, whose cytoplasm was densely filled with cell organelles. During next prenatal stage up to 260 mm fetus, the cytoplasm of the ganglion cells contained except for large pigment granules, all intracytoplasmic structures which were also found in mature superior cervical ganglion. A great number of synaptic contact zones between the cholinergic preganglionic axon and the dendrites of the postganglionic neuron were observed and a few axosomatic synapses were also observed. Two morphological types of the granule-containing cells in the superior cervical ganglion were first identified at 90 mm fetus. Type I granule-containing cell occurred in solitary, whereas type II tended to appeared in clusters near the blood capillaries. Synaptic contacts were first found on the solitary granule-containing cell at 150 mm fetus. Synaptic contacts between the soma of type I granule-containing cells and preganglionic axon termials were observed. In addition, synaptic junctions between the processes of the granule-containing cells and dendrites of postganglionic neuron were also observed from 150 mm fetus onward. In conclusion, superior cervical ganglion cells and granule-containing cells arise from a common undifferentiated cell precursor of neural crest. The granule-containg cells exhibit a local modulatory feedback system in the superior cervical ganglion and may serve as interneurons between the preganglionic and postganglionic cells.

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Ultrastructural Study on the Parenchymal Cell of Korean Planaria (Dugesia japonica) (한국산 플라나리아(Dugesia japonica Ichikawa et Kawakatsu) 유조직의 미세구조에 관한 연구)

  • 장남섭
    • The Korean Journal of Zoology
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    • v.30 no.1
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    • pp.53-70
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    • 1987
  • The morphological study on the parenchymal cells in the adult planaria performed to observe their cytochemical and ultrastructural characteristics. The results are as follows. Nine types of cells are found in parenchyma. 1. Free parenchymal cell: These cells contain several chromatoid bodies around the nucleus. Heterochromatins are evenly dispersed in large nucleus. These cells are abundant in free ribosomes. 2. Fixed parenchymal cells: These cells have well-developed granular endoplasmic reticulum, mitochondria and Golgi complex but they contain the cytosols exhibiting electron-lucencies. 3. Rhabdite-forming cells: These cells contain the electron-dense rhabdite granules of up to about 0.3 x 0.9 $\mu$m in size. Rhabdite-forming cells have well-developed cell organelles, granular endolplasmic reticulum, mitochondria and Golgy complex. 4. A-type of basophilic granule cells: These cells contain irregularly-shaped granules exhibiting alcianophilia. These granules surrounded by a limited membrane, approximately 1.4 x 0.7 $\mu$m in size, are accumulated in the cytoplasm. 5. C-type of basophilic granule cells: These cells contain electron-dense granules of less than 0.2 $\mu$m in size, which exhibit PAS- positive reaction. This type of granule is also found in the muscle layer of parenchyma. 6. D-type of basophilic granule cells: This type of granule cell occurs only in the parenchyma around reproductive organ. The granules have cytochemical characteristics that they exhibit strongly positive reaction with PAS and weakly eosinophilic property. These electron-dense granules, which are 0.2 to 0.6 $\mu$m in length, have oval shapes. 7. E-type of basophilic granule cells: These cells are found only in the parenchyma around re productive organ. The granules contained in a small number in the cell, exhibit PAS-positive reaction and have an average size of 0. 2pm. 8. Eosinophilic granule cells: These cells contain a large number of eosinophilic granules which have relatively diverse sizes from 0.3 x 0.2 to 0.8 x 0.4 $\mu$m. Most of granules are round or irregularly-shaped and highly electrondense. These cells have an array of well-developed granular endoplasmic reticulum of which cisternae are distened. 9. Transparent granule cells contain electron-lucent granules which exhibit negative reactions with three kinds of cytochemical methods used in this experiment.

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Effect of Structural Type of Clay Minerals on Physical Properties of Mountainous Grassland Soils

  • Choi, Seyeong;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.6
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    • pp.807-812
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    • 2016
  • Soil amendment, especially addition of clay minerals, has been widely conducted to improve the physical and chemical properties of cultivated soils. However, there are no systematic studies on the effects of the structural type of clay minerals added. This study was conducted to investigate the effects of structural type of clay minerals on physical properties of soils. Two experimental soils, layer-dominant and granule-dominant ones, were mixed with either a layer-type smectite or a granule-type zeolite at a level of 2.0 wt%. It was observed that water permeability of soils was decreased by smectite whereas not significantly changed by zeolite. This effect was much greater in layered clay-dominant soil than in granular clay-dominant soil. Our results clearly indicated that the relationship of structural type between a soil and an amendment plays a decisive role in the soil properties. Therefore, it is highly recommended that the structural types of both soil and amendment be taken into consideration for soil amendment by clay minerals.

The Effect of Phosphate Desorption Solution on LDH (Layered Double Hydroxide) Desorption (탈착 용액의 조성변화가 층상이중수산화물에 흡착된 인 탈착에 미치는 영향)

  • Jung, Yong-Jun
    • Journal of Korean Society on Water Environment
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    • v.24 no.6
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    • pp.670-675
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    • 2008
  • Batch type adsorption and desorption tests were performed with different types (Powder, Granule) of Layered double hydroxides (LDHs) saturated with phosphate. The adsorption isotherm was approximated as a modified Langmuir type equation. The maximum adsorption capacity was 55 mg-P/g-LDH for powder type LDH, and 46 mg-P/g-LDH for granule type LDH. The highest phosphate desorption (79.6%) was obtained with 20% NaOH solution, whereas the desorption degrees were 4.8, 22.2% and 46.7% in the solutions of acidic condition (pH 4), 30% NaCl, and 3% NaOH, respectively. It was suggested that the optimal condition for the phosphate desorption from LDH was 30% NaCl + 3~6% NaOH solutions. The desorption characteristics of LDH was little influenced by adsorbent type.

Effect of Morphology and Granule Size of Crystalline D-Sorbitol on Texture of Sugar-Free Chewing Gum (결정형 솔비톨의 형태 및 입자 크기가 무설탕 츄잉껌의 텍스쳐에 미치는 영향)

  • Kim, Sang-Yong;Oh, Deok-Kun
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.987-991
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    • 1997
  • Effect of morphology and granule size of crystalline D-sorbitol on texture of sugar-free chewing gum was investigated with different morphology of sorbitol such as a compact shaped P-type and a loose shaped S-type, and with different granule size such as 50 mesh, 80 mesh, and 100 mesh. The chewing gum including S-type sorbitol exhibited an increased flexibility and a decreased hardness as compared with that including P-type sorbitol. S-type sorbitol was chosen as a solid phase of sugar-free chewing gum because the consumer preferred a chewing gum with high flexibility and low hardness. The flexibility and hardness of chewing gum including S-type sorbitol were determined to be optimum at 80 mesh of the sorbitol size. The flexibility of the chewing gum including 80 mesh S-type sorbitol increased but the hardness decreased with increasing temperature.

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Preparation and Release Characterization of Osmotic Granule Nifedipine Delivery System (니페디핀 삼투성 과립 시스템의 제조와 약물 방출 특성)

  • Jeong, Sung-Chan;Cho, Young-Ho;Lee, Soo-Young;Lee, Bong;Kim, Moon-Suk;Kang, Gil-Son;Lee, Hai-Bang
    • Journal of Pharmaceutical Investigation
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    • v.36 no.1
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    • pp.11-17
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    • 2006
  • The objective of this study was to confirm the effect of the type of dissolution media and paddle speed on nifedipine (ND) release profile from osmotic granule and the storage stability. Osmotic granule was manufactured by fluidized bed coating method. At each coating step, morphology of osmotic granule was differed. The size of osmotic granule was $750\;{\mu}m$ at 3 wt% membrane thickness. ND release was changed in diverse dissolution media, paddle speed. ND release is governed by not only osmotic pressure but diffusion from osmotic granule. ND release from osmotic granule decreased as storage period increased. These may be caused by liquid excipient which has low molecular weight. Storage stability of osmotic granule could be improved by removing liquid excipient from semipermeable membrane.

Identification of Irradiated Granule-Type Ramen Soup Powder by Pulsed Photostimulated Luminescence and Thermoluminescence during Storage

  • Yi, Sang-Duk;Yang, Jae-Seung;Kim, Dong-Woo;Shin, Doo-Ho;Jo, Gab-Yeon;Chang, Kyu-Seob;Oh, Man-Jin
    • Preventive Nutrition and Food Science
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    • v.8 no.1
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    • pp.79-84
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    • 2003
  • This study was carried out to establish a method for determining if granule-type Ramen soup powder has been irradiated. Thermoluminescence (TL) and pulled photostimulated luminescence (PPSL) were used as the detection methods through observed changes of TL and PPSL intensities after storage under differing conditions. PPSL intensities increased with increases in irradiation doses. The threshold level of PPSL was below 412$\pm$58 photon counts regardless of storage conditions (room and darkroom) after 10 months. TL intensities also increased with increasing irradiation doses. The coefficients ($R^2$) of PPSL (0.74~0.94) and TL intensities (0.92~0.58) were very highly correlated with irradiation dose. The PPSL and TL intensities were decreased after 10 months of storage. These results indicate that discrimination of irradiated from non-irradiated granule-type Ramen soup powder is possible using TL and PPSL methods despite the decrease in intensities of TL and PPSL with increasing storage times.

A Study on the Ultrastructure of the Integumental Epidermis of Korean Planaria (Dugesia japonica Ichikawa et Kawakatsu) (韓國産 플라나리아(Dygesia japonica Ichikawa et Kawakatsu) 表皮上皮細胞의 微細構造에 關한 硏究)

  • 장남섭;김우갑
    • The Korean Journal of Zoology
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    • v.28 no.3
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    • pp.194-208
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    • 1985
  • The ultrastructure of the integumental epidermis of Korean planaria (Dugesia japonica) is studied by light microscope, scanning electron microscope and transmission electron microscope. The planaria has mono-layered integumental epidermis in which most of cells exhibit irregularly columnar shape. The epidermal cells of the integument are classified into six types on the basis of cytochemical and ultrastructural characteristics. 1) Ciliated epithelial cells: These cells have cilia in their free surfaces. The axonemes of cilia exhibits fundamental 9+2 microtubular pattern. 2) Eosinophilic cells: These cells contain a few large eosinophilic granules. The core of eosinophilic granule is consisted of sparsely dispersed fibrillar structures in relatively electron-lucent ground material. 3) Mucous cells: These cells are filled with irregularly shaped, PAS-positive mucous granules which have an average size of $0.8\\times0.3 \\muM$. 4) Rhabdite-forming cells: These cells possess a few strongly-eosinophilic large rhabdite granules. The rhabdite granules are synthesized either in the rhabdite-forming cells which constitute integumental epidermis or in the corresponding cells which are developed in the parenchyma and later transferred to epidermal cells of integumental epidermis through basement membrane. 5) A-type of basophilic granule cells: These granule cells possess round or irregularly-shaped granules which are strongly stained with Alcian blue. These electron-dense granules have an average size of $1.5\\times1.0 \\muM$. This type of cells is derived from parenchymal tissue. 6) B-type of basophilic granule cells: These basophilic granule cells with PAS-positive granules, are found in the epidermis of lateral body wall. The granules, which are about $0.7\\times0.4 \\muM$ in size, occupying most part of this cell type are originated from the parenchyma.

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Development of Variable Rate Granule Applicator for Environment-Friendly Precision Agriculture (I) - Concept Design of Variable Rate Pneumatic Granule Applicator and Manufacture of Prototype - (친환경 정밀농업을 위한 입제 변량살포기 개발 (I) - 송풍식 입제 변량살포기 개념설계와 시작기 제작 -)

  • Ryu K.H.;Kim Y.J.;Cho S.I.;Rhee J.Y.
    • Journal of Biosystems Engineering
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    • v.31 no.4 s.117
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    • pp.305-314
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    • 2006
  • Precision farming has been known as an environment friendly farming technology. This study was conducted to develop a variable-rate granule fertilizer applicator as an attempt for introducing the precision farming technologies to rice cultivation in Korea. In this paper, concept design process and manufacturing of prototype variable rate granule applicator was reported. For concept design, some design guide lines were selected. Based on the design guide line and some engineering knowledge, concept design was conducted. The designed prototype granule applicator was mounted at the rear of riding type cultivator for paddy field and had a 10m wide boom structure with pneumatic conveying and application system as well as 1GPS receiver, 1 granule hopper, 12 blow heads, 2 metering devices and 1 controller. The fertilizer applicator had 942 ka of weight, 740m of ground clearance and 1,117mm of center of gravity from the ground. The applicator was designed to be able to $34{\sim}428kg/ha$ of granule at $0.2{\sim}0.8m/s$ of fertilizer working speed.