The objective of this study was to determine the effects of low temperature on starch accumulation in rice grains. We used four major Japonica-type Korean rice cultivars as materials: Jinbu (JB), Junamjosaeng (JJ), Geumyoung (GY), and Hwawang (HW). Rice plants were moved into two phytotrons the day after heading. Temperatures in the two phytotrons were maintained at $19/29^{\circ}C$ (night/day) as the control, and $13/23^{\circ}C$ as the low temperature condition, both under natural daylight with a relative humidity of 65%. The ripening rates of JB and JJ showed no significant difference between the low temperature and control conditions at 45 days after heading (DAH). In contrast, the ripening rates of GY and HW were 86% and 57% lower than those of JB and JJ under the low temperature condition at 45 DAH, respectively. However, the ripening rates of these four varieties at 61 DAH (when accumulated temperature reached $1,100^{\circ}C$) under the low temperature condition were similar to those at 45 DAH under the control condition (JB, 94%; JJ, 97%; GY, 97%; HW, 88%). The total starch contents showed no significant difference between the control and low temperature conditions. However, the amylose contents in the cultivars were higher under the low temperature than under the control condition. The enzyme activities of starch biosynthesis were about 5-10 days slower in cultivars under the low temperature than under the control. The grain-filling rate showed significant correlations with the enzyme activities of SuSase ($r^2=0.70^{***}$), AGPase ($r^2=0.63^{***}$), UDPase ($r^2=0.36^{***}$), StSase ($r^2=0.51^{***}$), and SBE ($r^2=0.59^{***}$). In conclusion, although StSase activity was increased at $13/23^{\circ}C$ up to 20 DAH, there might not be enough time for SBE to synthesize amylopectin, thus affecting the amylose content of HW, which had the slowest grain filling rate. Notably, the decreased activity of SuSase and SBE and late increase in AGPase activity under the low temperature during the ripening stage are considered to be disadvantageous, as they delay ripening and increase the amylose content.
Persisent circadian rhythms in carbon assimilation, stomatal conductance and soluble carbohydrate concentration were investigated during grain filling period in rice plant transferred from a natural photoperiod to constant conditions. A weak rhythm in photosynthesis, measured as carbon assimilation, and stomatal opening, as conductance to water vapor, with a period of approximately 24-hours, occurred under constant condition. Carbon assimilation and stomatal conductance reached maximum values near noon and minimum values near midnight during the early stage (until 72-hour) after transferring to constant condition, and then the amplitude and phase were changed slowly, the rhythms with little damping, reaching maximum values near midnight and minimum values near noon during 96~120-hours after transferring. However, photosynthesis in plants grown for 14days after anthesis under constant moderate light(day and night) did not oscillated in constant condition unlike plants grown under a cycle of light and darkness. These phenomenon was observed in soluble carbohydrate concentration in flag leaves as well. Evidences from several approaches indicate that endogenous rhythms of $CO_2$ assimilation, stomatal conductance and soluble carbohydrate concentration are closely couped with each other and particularly important to plants, which depend on the natural day-night cycle as a external signal.
Korean Journal of Agricultural and Forest Meteorology
/
v.2
no.4
/
pp.148-155
/
2000
The typhoon and flooding injury in rice were investigated both the region of downpour at the middle of August and the region of typhoon 'Yanni' at the late of September, 1998 in Kyongbuk provincial of Korea. More than 10% of rice stem were buried by soil inundation, the heading was delayed and the high node tiller was brought out. Yield components were deteriorated and yield was reduced. As the clay in the muddy water was attached on grains of rice, the yield was greatly reduced by reason of low grain filling ratio and polished rice ratio. The injury of muddy water was less at yellow ripening stage than at early ripening stage. On the other hand, the muddy water injury was reduced by the fungicide 'Benomyl' and 'Daconil' treatments. The degree of lodging according to varieties and ripening stages was not made a great difference, while the lodging was mainly occurred in the early transplanting time. In a week after lodging, viviparous spikes were occurred in almost all varieties, the degree of viviparity was shown highly in order of early maturing, middle maturing and late maturing varieties in the field as well as in laboratory test. The viviperous spikes were greatly occurred in the lodging toward hill space within row than row space because the more grains were touched on the ground.
The stability of rice cultivation in Korea is largely depended on climatic conditions, especially, low temperature at the period of early growth stage and after heading. The improvement of cold tolerant varieties and appropriate cultural practices in rice are very effective to minimize the cold damage. This paper is summarized the mechanism and counterplans of cold injury of rice plants. The paddy area having commonly cold injury in Korea is approximately 15, 522ha in 1,709 sites on the national scale. The cold damage at seedling stage in nursery bed appeared to poor germination, leaf discoloration, dead seedlings and seedling rot ect.. At the vegetative stage, the decreased tiller number due to poor rooting and the delayed heading caused by slow growth and panicle differentiation are commonly showed. The cold injury at early reproductive stage appeared to the degeneration of spikelets and rachis - branches, while that at meiosis stage showed to increased sterility due to poor development of pollen and shortened panicle length with delaying heading, therefore the grain yield is largely decreased. The cold damage at heading and ripening stages showed to poor pollination and fertilization, low panicle exsertion, poor grain filling and finally grain quality became low. To minimize the cold injury to rice plants by low temperature, following counterplans would be recommonded ; Improvement of the cold toelrant rice varieties for the regions of midmountains and alpines. Raising healthy seedlings at upland nursery beds and by using of growth regulators such as ABA, Fuchiwang and Tachiace. Soil improvement and organic matter application to reduce cold damage by increasing water and fertilizer holding capacities in the paddy field having commonly cold water and in the place where cold damage is regularly occurred. Appropriate fertilization for raising healthy rice plants to tolerate under low temperature condition. Water management to increase water temperature in the paddy such as depth watering, round channels and polyethylene tubes around the field. Establishment of the optimum cultivation time of rice based on minimum, mean and maximum temperatures at different regions with appropriate rice varieties.
To study the effect of cold water irrigation at the reproductive growth stage of rice plants on several growth characters related to source and sink and nutrient uptake, the present experiment was carried out under the different conditions of water temperature and water depth. Deep irrigation with normal temperature water increased culm length, panicle length and panicle exsertion but with cold water resulted adversely. Most sensi-tive response in 5 cm water-depth appeared at reduction division stage and in 20 cm depth at panicle formation stage. Secondary branches and spikelets were increased in number by deep irrigation with normal temperature water, but decreased and degenerated by deep irrigation with cold water at panicle formation stage resulting in high spikelet sterility and low grain filling. Deep irrigation with normal temperature water increased the contents of total nitrogen, phosphate, potassium and silicate in leaf blades, branches and chaff. However, cold water irrigation reduced the uptake of phosphate, potassium and silicate except nitrogen particularly in deep irrigation. Ratios of phosphate, potassium and silicate to total nitrogen content were decreased by cold water irrigation. Branches seemed to have higher requirements for phosphate, potassium and silicate than leaf blades and chaff. Silicate-to-total nitrogen ratio in leaf blades, branches and chaff had significant correlations with yield showing closer relationship between yield and the ratio of silicate to total nitrogen in branches in particu-lar.
Coming with the well-being era, consumer's demand for safe agricultural products is increasing. So, it is urgent to develop an environment-friendly rice production system. Accordingly, this study was conducted to develop an environment-friendly rice production system by using Amo known as EM(effective microorganisms) and also known as being effective in environment-friendly rice production with several other environment-friendly agricultural materials. The highest number of tillers per hill was obtained from level 2 of EM treatment (48.8 tillers per hill), while the lowest was obtained from the control plot (41.0 tillers per hill). Leaf area per hill at heading stage was the highest in level 3 of EM treatment $(3228.5cm^2)$, while control was the lowest leaf area $(2264.7cm^2)$, which is 70.2% compared to the level 3 of EM treatment. The highest effective tillers was obtained from the control (63.7%), while the lowest effective tillers was obtained from the level 3 of EM treatment (55.4%), which were treated with higher amounts of environment-friendly agricultural materials. Level 3 of EM treatment showed the highest number of panicles per hill (20.9), while the control showed the lowest number of panicles per hill (19.3). In the spikelets per panicle, level 2 of EM treatment showed the highest number of spikelets (85.2), while the control showed the lowest number of spikelets (81.9) and there was a statistically significant difference among the three levels and control. The highest grain filling ratio was obtained from the control (85.0%), while level 3 of EM treatment was the lowest grain filling ratio and there were no great difference between treatment levels. Regarding the 1000 grain weight, the control showed the highest 1000 grain weight (21.7g), which is heavier by about 1g compared to treatment levels. Level 2 of EM treatment showed the highest rough rice yield per 10a, while level 3 of EM treatment was the lowest and they also showed a statistically significant difference among treatment levels.
Five soybean varieties of two early maturing; Karikei 73 and SS79168, and three late maturing; Tohoku 76, Baegunkong and Jangbaegkong, were used and evaluated in the study, Of the varieties examined, Karikei 73 was characterized by the delayed leaf senescence, To investigate the periodical trends of sugars in plant organs and their roles to the nitrogen fixing activity of root nodules, the concentrations of reducing and non-reducing sugar in root and nodules during the grain filling period were measured. The concentration of non-reducing sugar in roots was not changed up to the stage of R6 for all of the varieties but it decreased rapidly thereafter for the varieties except Karikei 73. No such rapid decrease in the concentration of non-reducing sugar in the roots was observed for Karikei 73 having the characteristics of delayed leaf senescence. The concentration of reducing sugar in the root nodules was not greatly changed for all of the varieties up to the stage of R6 regardless of the earliness of varieties but increased temporarily at R6.5 when there was a rapid decrease in ARA. The phenomenon explained the fact that nitrogen fixing activities were controlled not only by supplying sugars as the source of energy for nitrogen fixation, but also by the need for fixed nitrogen of the plant. The concentration of non-reducing sugar in root nodules also increased up to the stage of R6-R6.5 but decreased at R7, which could apply the same explanation as in the concentration of reducing sugar of the root nodules.
Park, Sung-Tae;Hwang, Chung-Dong;Hwang, Dong-Yong;Son, Yang;Kim, Soon-Chul;Oh, Yun-Jin
Korean Journal of Weed Science
/
v.16
no.3
/
pp.181-186
/
1996
This experiment was conducted at National Yeongnam Agricultural Experiment Station in 1995 to identify effects of early flooding in dry seeded rice on rice growth and weed occurrence. The early flooding of emergence date and the 2-leaf stage were not differed significantly on seedling stand, seedling growth of rice and plant characteristics associated with lodging compared to flooding of the 4-leaf stage. However, weed occurrence resulted in 39-61% suppression. Most predominant weed regardless of flooding times was Echinochloa crus-galli, followed by Aneilema japonicum. The Aneilema japonicum, in general, was higher occurrence with earlier flooding, while dry land weeds such as Digitaria sanguinalis, Stellaria alsine etc. reduced. Grain yield of early flooding at emergence date and the 2-leaf stage was higher by 2-5% than that of the 4-leaf stage due to better grain filling ratio and heavier 1,000 grain weight.
Tungsten (W) thin film was deposited at $400^{\circ}C$ using pulsed chemical vapor deposition (pulsed CVD); film was then evaluated as a nucleation layer for W-plug deposition at the contact, with an ultrahigh aspect ratio of about 14~15 (top opening diameter: 240~250 nm, bottom diameter: 98~100 nm) for dynamic random access memory. The deposition stage of pulsed CVD has four steps resulting in one deposition cycle: (1) Reaction of $WF_6$ with $SiH_4$. (2) Inert gas purge. (3) $SiH_4$ exposure without $WF_6$ supply. (4) Inert gas purge while conventional CVD consists of the continuous reaction of $WF_6$ and $SiH_4$. The pulsed CVD-W film showed better conformality at contacts compared to that of conventional CVD-W nucleation layer. It was found that resistivities of films deposited by pulsed CVD were closely related with the phases formed and with the microstructure, as characterized by the grain size. A lower contact resistance was obtained by using pulsed CVD-W film as a nucleation layer compared to that of the conventional CVD-W nucleation layer, even though the former has a higher resistivity (${\sim}100{\mu}{\Omega}-cm$) than that of the latter (${\sim}25{\mu}{\Omega}-cm$). The plan-view scanning electron microscopy images after focused ion beam milling showed that the lower contact resistance of the pulsed CVD-W based W-plug fill scheme was mainly due to its better plug filling capability.
Due to the strong influence of nitrogen(N) on plant productivity, a vast amount of N fertilizers is used to maximize crop yield. Over-use of N fertilizers leads to severe pollution of the environment, especially the aquatic ecosystem, as well as reducing farmer's income. Growing of N-efficient cultivars is an important prerequisite for integrated nutrient management strategies in both low- and high-input agriculture. Taking maize as a sample crop, this paper reviews the response of plants to low N stress, the physiological processes which may control N-use efficiency in low-N input conditions, and the genetic and molecular biological aspects of N-use efficiency. Since the harvest index(HI) of modern cultivars is quite high, further improvement of these cultivars to adapt to low N soils should aim to increase their capacity to accumulate N at low N levels. To achieve this goal, establishment and maintenance of a large root system during the growth period may be essential. To reduce the cost of N and carbon for root growth, a strong response of lateral root growth to nitrate-rich patches may be desired. Furthermore, a large proportion of N accumulated in roots at early growth stages should be remobilized for grain growth in the late filling stage to increase N-utilization efficiency. Some QTLs and genes related to maize yield as well as root traits have been identified. However, their significance in improving maize NUE at low N inputs in the field need to be elucidated.
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