• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.21-29
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• 2005

This study was done to obtain their morphological traits to analyse genetic diversity and intraspecific relationship of 47 Polygonatum species collected from Gyeongnam province. Plant height was the highest in P. thunbergii but the shortest in P. involucratum. Growth habit and its colors were classified to 3 groups, respectively. Leaf shapes were sorted to 5 groups including lanceolate with petiole or none, petiole colors were done to 3 groups including a species having dark green leaves of purple colored margin. Flower shapes were divided as 3 groups of urceolate, tubular and gourd shapes, and its colors were white, greenish white and light green, especially light green in a species with gourd shape. Filament shapes were two types of flatness and cylinder. Peduncle color and bract attached below it showed 4 types, respectively. Fruit shapes were sorted to 3 groups. In 100-fruit weights P. ordoratum var. pluriflorum showed the greatest but P. involucratum did the least. Two species were completely resistant to leaf brightness although 7 species showed less than 7 % infection rates. Rhizome yields ranged from 4.4 g to 94.8 g per plant, showing their significant variation. In correlation analysis between 9 major characters, rhizome yield per plant was positively correlated with plant height, stalk diameter, leaf number, leaf length and width, and rhizome diameter but leaf brightness was negatively done with plant height, stalk diameter, leaf number and length, 100-seed weight, rhizome yield per plan and rhizome diameter.

• Research in Plant Disease
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• v.22 no.3
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• pp.158-167
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• 2016

A pathogen that causes a new disease on green pumpkin in the nursery and the field was characterized and identified. Symptoms of the disease on green pumpkin were water soaking lesions and spots with strong yellow halo on leaf, brown lesion on flower, and yellow spot on fruit. The bacterial isolates from the leaf spot were pathogenic on the 8 curcubitaceae crop plants, green pumpkin, figleaf gourd, wax gourd, young pumpkin, zucchini, cucumber, melon, and oriental melon, whereas they did not cause the disease on sweet pumpkin and watermelon. They were Gram-negative, rod shape with polar flagella, fluorescent on King's B agar and LOPAT group 1a by LOPAT test. Their Biolog substrate utilization patterns were similar to Pseudomonas syringae pv. syringae's in Biolog database. Phylogenetic trees with 16S rRNA gene sequences and multilocus sequence typing (MLST) with nucleotide sequences of 4 housekeeping genes, gapA, gltA, gyrB, rpoD and those of P. syringae complex strains in the Plant Associated and Environmental Microbes Database (PAMDB) showed that the green pumpkin isolates formed in the same clade with P. syringae pv. syringae strains. The clade in MLST tree was in the genomospecies 1 group. The phenotypic and genotypic characteristics suggested that the isolates from green pumpkin lesion were P. syringae pv. syringae.

• Journal of Nutrition and Health
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• v.38 no.10
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• pp.801-806
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• 2005

This study was designed to examine the effects of fractions of ethanol extract of Benincasa hispida (wax gourd) on plasma glucose and lipid levels in streptozotocin (STZ) induced diabolic rats. sprague-Dawley rats were induced diabetes mellitus by STZ injection (45 mg/kg) into the tail vein and were divided into 5 groups: normal, STZ-control, three experimental diabetic groups. fractions of ethanol extract of Benincasa hispida were administered orally into the diabetic rats for 14 days. The food intake and body weight were monitored and plasma levels of glucose, cholesterol, HDL-cholesterol, triglyceride (TG) , free fatty acid (FFA) , and aminotransferase activity were analyzed. A significant lowering effects of plasma glucose levels were observed in the chloroform fraction and $H_2O$ fraction group compared to STZ-control group at 14 days. Administrations of each of the three fractions decreased plasma TG and FFA levels in diabetic rats. Activity of alanine aminotransferase (ALT) in $CHCl_3$ fraction and $H_2O$ fraction groups and aspartate aminotransferase (AST) in $H_2O$ fraction group significantly lower than STZ-control group. The results show that $CHCl_3$ fraction of Benincasa hispida could be effective to control the STZ-induced diabetic rats. (Korean J Nutrition 38(10): 801$\sim$806,2005)

• The Plant Pathology Journal
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• v.15 no.1
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• pp.72-75
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• 1999

A scab disease occurred on watermelon seedlings by the infection of Cladosporium cucumberinum. This is the first report demonstrating the scab disease on watermelon in Korea. The casual agent attacked all plant parts of the seedlings on the ground. Infection sites were initiated with sunken and dark green spots, and then suddenly developed to large lesions softened with gummy substances. When hypocotyl and leaf stalk of seedlings were infected and softened, upper parts of seedlings were brokin down, dried and eventually died. Conidiophores of the fungus were characterized by pale olivaceous brown color, and variable length of about 3-5 mm in width. Conidia were formed I long branched chains, and conidium was ellipsoidal, fusiform or subspherical single cell mostly without septum. Morphological characteristics of the fungus were almost identical to Cladosporium cucumerinum Ellis & Authur. The fungus was also pathogenic to cucumber, squash and oriental melon, suggesting that it is a common pathogen to cucurbits. However, the fungus was not pathogenic to bottle gourd.

• The Plant Pathology Journal
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• v.17 no.5
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• pp.243-248
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• 2001

Two Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV) and Zucchini green mottle mosaic virus (ZGMMV), occurred in Korea in 463 ha in 1998, 33.9 ha in 1999, and 44.2 ha in 2000. CGMMV was detected in watermelon, cucumber, oriental melon, and melon, whereas ZGMMV was mainly detected in zucchini squash. Thirty-six CGMMV isolates wee classified into three types by analysis of single strand cDNA conformational polymorphism (SSCP) of the coat protein gene. In a comparison of serological relationships among CGMMV, ZGMMV, and Kyuri green mottle mosaic virus (KGMMV), the three tobamoviruses specifically reacted with each homologous antibody in the double-antibody sandwich enzyme-linked immunosorbent assay and rapid imunofilter paper assay (RIPA), although ZGMMV and KGMMV were slightly biologcially similar. In a survey of the three tobamoviruses in cucurbitgrowing field in Korea by RIPA, CGMMV and ZGMMV were detected but KGMMV was not found in commercially growing cucurbit crops so far. Seed contamination ratio of CGMMV in bottle gourd seeds tested was 84%, while seed trasmission ratio from the virus-contaminated seeds was 2.0%. Soil transmission ratio was 0-3.5% in fields naturally infested with CGMMV or ZGMMV. Control measures of the virus diseases are roguing and sanitation. These suggest that it is important to rogue the first infected crops, which include the seed and soil, especially early in the season. This may be practicable to control the diseases because CGMMV and ZGMMV have a narrow host range restricted to cucurbitaceous crops.

• Journal of Conservation Science
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• v.19
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• pp.31-42
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• 2006

There are many materials used in restoring missing parts of pottery and ceramics and they are used according to the each quality of materials. In our research we tried to find the new repair materials to solve many problems exposed in the existing repair materials. As a result, we made new repair materials that we properly put oven bake clays into repair epoxy putty which is being used as oven bake clays for a long time. Fortunately, through testing yellowing, vickers hardness, coloring and heating temperature, We could get an idea that new repair materials would be the best materials in the efficiency of restoration, yellowing, and coloring.

• Korean Journal of Ichthyology
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• v.24 no.4
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• pp.253-262
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• 2012

A skeletal system of Coreoleuciscus splendidus from Seomjin and Mankyeong Rivers is described and figured in detail. The osteological characteristics are as follow: preorbital area elongated; eight proximal radials on the anal fin; 1st basibranchial bone with a bottle gourd shape; two types of pharynegeal teeth; intercalarium's ascending process absent and rib 4 broad greatly. Meanwhile, the number of actinost and the length of external process and internal process on the pelvic girdle are variable with individuals. These characteristics are shown in the two population. The epural on the caudal skeleton, however, has two types only in C. splendidus of Mankyeong River.

• The Korean Journal of Food And Nutrition
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• v.6 no.1
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• pp.16-24
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• 1993

The "Namul" is a Korean common vegetable food composed of edible young leaves and soft stalks of wild plants or cultivating vegetables and treated with traditional condiments. It has been widely used as not only a regular diet but seasonal foods or special ceremonial foods. In this thesis, the kinds of edible plants for namul were philologically approached by books published in Korea from Koryo to Chosun dynasty. The first historical record about cultivated vegetable as food in Korea was a garlic and a gourd in $\ulcorner$Samkuksaki$\lrcorner$. According to numerous records, edible wild plants might have played an important part as food resources, since they have variable edible portions as famine relief foods. Four kinds of namul were first introduced in Koryo age, but in Chosun dynasty over eighty kinds were recorded. The root of ballonflower and white radish were most commonly used for raw namul and bamboo shoot and squash for boils ones. Most kinds of namul were introduced in $\ulcorner$Zeungtosanrimkwungiae$\lrcorner$ and $\ulcorner$Limwonsiprwukji$\lrcorner$ influenced by pragmatism in later half period of Chosun. The chronic state of famine in later half period of Chosun caused to introduce various edible wild vegetables, and had greatly influenced on the Korean people to acquire vegetarian food habits and to favor salty taste. It seems to need a further study on recent nutritional problems including dietary fiber.ary fiber.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.397-401
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• 1998

Asexual spores of Mycosphaerella nawae were profusely produced on PDA after a prolonged incubation at $25^{\circ}C$ for 90 days. When persimmon trees were artificially inoculated by the conidial suspension, typical symptoms of circular leaf spot of persimmon appeared on the leaves two month later. The imperfect stag of the fungus was identified as Ramularia sp. based on following morphological characteristics examined under a light microscope and a scanning electron microscope. Conidia were mostly ellipsoid, but occasionally cylindrical, elongated oval, taro, peanut or gourd shapes and measured as 12.2~32.6$\times$6.1~10.2 ${\mu}{\textrm}{m}$. erect, hyaline, colorless-light brown. Conidia were formed solitarily or in chains on a medium and infected leaves. Conidiophore was erect, hyaline, colorless-light brown. and the size was 20.4~102.0$\times$3.1~10.2 ${\mu}{\textrm}{m}$, respectively. In this paper, we firstly demonstratrated that asexual spores of M. nawae induced persimmon circular leaf spot in nature as well as sexual spores of the fungus. Therefore, it is hypothesized that the imperfect stage of the fungus plays an important role in nature for epidemics as secondary inoculum.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.62-65
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• 2004

To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.