• 한국양식학회지
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• 제16권4호
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• pp.240-244
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• 2003

양식산 넙치를 대상으로 아치사 만성농도의 PCBs가 자어기의 원시생식소와 신장의 발달에 미치는 영향을 살펴보았다. 원시생식소 발달 과정은 4단계; 원시생식세포(primodial germ cell, PGC) 단계, 생식융기 (genital ridge type, GRT) 단계, 원시생식소 I형(primitive gonad type I, PGT I) 단계 및 원시생식소 II형(primitive gonad type II, PGT II) 단계로 구분할 수 있었다. 시원생식세포는 부화 후 3일에 발견되었고, 원시 생식소는 대조구에서 부화 후 24일에 나타나며 처리구에서는 부화 후 22일에 나타났다. 신장 발달과정은 4단계; 단일관형 중신관(unitubular type of mesonephric duct, UTMD) 단계, 분지형 중신관(the branched mesonephric duct, BMD) 단계, 세뇨관 형성(convoluted tubule formation CTF) 단계 및 사구체 출현(glomerulus appearance, GA) 단계로 구분할 수 있었으며, 신장의 구조적 완성시기는 대조구와 처리구 모두 부화 후 25∼30일로 나타났다. 하지만 원시 생식소와 신장 모두 대조구와 처리구에서 유의한 차를 보이지 않았다 (P>0.05). 그러므로, 넙치 자어기의 원시 생식소와 신장의 발달에 따른 PCBs(3.0 $\mu\textrm{g}$/L)의 영향은 없었다.

• 한국가금학회지
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• 제23권2호
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• pp.61-69
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• 1996

Primordial germ cells (PGCs) were manipulated as part of the system to produce transgenic chickens. PGCs were isolated from the germinal crescent of developmental stage 6 to 8 donor emhryos of the Korean Native Ogol Chickens (KNOC). These PGCs were transfected with plasmid DNA containing the lac Z gene by liposome mediated transfection methods. The lac Z gene was transfected and expressed in the PGCs. These transfected PGCs were injected into the germinal crescent of White Leghorn embryos (stage 6 to 8). The injected transfected PGCs migrated via the circulatory system into the future gonad and expression observed in the gonads of 3 day old chick. Of the 47 embryos and 3 day old chickens, one positive PGCs gonad from sacrificed young chickens was detected by appearance of blue cells. Plasmid DNA with the foreign gene was incorporated into the population of germ cells in the gonad. These results demonstrate that PGCs can he transfected and then transferred for colonization into the gonad, and show the potential to ultimately manipulate the genetic material of the chicken gernline.

• 한국수산과학회지
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• 제39권5호
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• pp.398-403
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• 2006

Gonad development and the gametogenic cycle of pen shells, Atrina pectinata, collected from November 2004 to October 2005 in the coastal waters of Jangheung, Jeollanam-do, Korea were investigated histologically. The clams are dioecious. The gonads consist of a number of gametogenic follicles. The sex ratio (Female:male) was 1:1.2. The gonad index (GI) reached a maximum in May and a minimum in July. The condition index (CI) reached a maximum in April and a minimum in June. The gonadosomatic index (GSI), GI and CI all increased with gonad development. The gametogenic cycle of the clam could be divided into five stages: the degenerative/inactive (August to October), early active (November to March), late active (December to April), ripe (May to June) and spent (June to July) stage.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.299-303
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• 2021

The ascidian Halocynthia aurantium (sea peach), a marine invertebrate, belongs to the same genus of the phylum Chordata along with the ascidian Halocynthia roretzi (sea pineapple), which is one of the model animals in the field of developmental biology. The characteristics of development and reproduction of H. aurantium are not yet known in detail. In order to find out the spawning period of H. aurantium, we investigated development of the gonads during the annual reproductive cycle. Testis and ovary were both in the bisexual gonads (ovotestes) of H. aurantium, which is a hermaphrodite like H. roretzi. In H. aurantium, the right gonad was longer and slightly larger than the left gonad throughout the year. In each gonad, the number of the testis gonoducts was slightly higher than that of the ovary gonoducts. These features were similarly observed in H. roretzi. However, the number of the testis gonoducts and the ovary gonoducts in each gonad of H. aurantium was about half that of H. roretzi. The gonads of H. aurantium contracted during the winter and summer seasons. The gonads decreased to the smallest size around February, and then started to increase again in March. The gonads were most developed in September of the year. Therefore, it is estimated that the spawning of H. aurantium begins around this period.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.16.1-16.8
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• 2016

To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at $28^{\circ}C$. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.

• 한국수산과학회지
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• 제8권4호
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• pp.234-241
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• 1975

여수근해산 한전복 Haliotis discus hannai를 재료로 1973년 1월-12월까지 매월 1회씩 24회에 걸쳐 생식선열도지수, 성열도계수, 비만도등의 월변화와 각장 및 전중량의 생식선중량과의 관계, 수온조건 및 자연산모패의 산란유발 결과를 요약하면 다음과 같다. 1. 여수근해산 한전복의 산란가능기간은 5월 하순-7월하순과 9월상순-11월하순이며, 주요산란기는 5월하순-6월중순과 9월하순-11월하순으로 각각 추정되었다. 2. 생식선성열도계수가 극대치를 보인 것은 5월의 44.10과 9월의 49.05였고, 최소치는 1월의 10.09였다. 3. 비만도는 년중 7월이 0.221로 가장 높았으며, 10월에 0.1058로 가장 낮았다. 4. 각장(SL)에 대한 생식선중량(GW)의 비가 년중 가장 높았던 달은 9월이었고, 가장 낮았던 달은 1월로서 이때의 관계식은 9월 : $GW=0.0000003710SL^{3.7860}$ 1월 : $GW=0.000021198SL^{2.8564}$ 각년평균치 : $GW=0.0000005013SL^{4.2273}$ 으로 각각 표시되었다. 5. 전중량(TW)에 대한 생식선중량(GW)의 비가 년중 가장 높았던 달은 9월이었고, 가장 낮았던 달은 1월로서 이때의 관계식은 9 月 : GW=0.1918TW-4.9231 1 月 : GW=0.0992TW-0.1759 주년평균수 : GW=0.1568TW-3.5236으로 표시되었다.

• 한국방사선학회논문지
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• 제15권2호
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• pp.109-115
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• 2021

생식선 차폐를 하지 않았을 때 전부, 양 측부, 후부, 생식선 부위의 산란선을 측정하고 Xenolite nolead Apron(0.35 mm Pb), Xenolite nolead Apron(front 0.35 mm Pb Mix back 0.25 mm Pb, Skirt overlap), Half Apron(0.5 mm Pb)로 차폐 후 각각의 산란 선량을 측정하였다. 흉부 전산화 단층촬영 검사 시 검사 부위의 산란 선량은 272 μSv가 측정되었고, Apron으로 차폐하지 않았을 시에 평균 전부 43 μSv, 좌측부 81 μSv, 우측부 82μSv, 후부 38.8 μSv, Gonad 부위 16 μSv로 측정되었다. Xenolite nolead Apron으로 위쪽 부분만 차폐하고 측정했을 전부 11.2 μSv, 좌측부 43.1 μSv, 우측부 45.3 μSv, 후부 12 μSv, Gonad 부위 5.2 μSv로 측정되었다. Xenolite nolead Apron(Skirt overlap)으로 Pelvis 부위를 360° 감싼 후 선량을 측정하였을 때 전부 5.6 μSv, 좌측부 22.4 μSv, 우측부 15.7 μSv, 후부 6 μSv, Gonad 부위 3.2 μSv로 측정되었다. Xenolite nolead Apron(Skirt overlap)으로 Pelvis 부위를 360° 감싼 후 선량을 측정하였을 때 전부 5.6 μSv, 좌측부 22.4 μSv, 우측부 15.7μSv, 후부 6 μSv, Gonad 위 3.2 μSv로 측정되었다. Half Apron으로 위쪽만 차폐하고 측정했을 때에는 전부 10.7 μSv, 좌측부 42.6 μSv, 우측부 40.6 μSv, 후부 11.3 μSv, Gonad부위 4.7 μSv로 측정되었다. 골반 부위를 360° 차폐하는 방법이 80% 이상 선량 감소를 보였고, 전부 차폐 시 70% 이상의 선량 감소 효과를 보였으며 모든 전산화 단층 촬영 검사에 있어 피폭선량 경감을 위한 연구와 차폐 가능한 장비를 활용하는 다양한 기법에 관한 지속적인 연구가 필요하리라 사료된다.

• 디지털융복합연구
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• 제11권7호
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• pp.237-242
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• 2013

최근 임상에서 사용하고 있는 128채널 MDCT의 복부 및 골반부 촬영 시 생식선 차폐 선량관리 자료로 활용하기 위하여 생식선의 피폭선량과 생식선 차폐 시 피폭선량을 측정하고 획득영상을 평가하였다. 평가한 결과 복부 MDCT 검사시생식선 차폐기구를 사용하지 않았을 경우에서 $16.5{\pm}0.5$ mGy를 나타냈으며, 대($650m^2$)크기의 차폐체를 사용하였을 경우 $7.5{\pm}0.3$ mGy로 측정되어 54%의 생식선 피폭선량 감소효과를 보였다. 골반부 MDCT 검사시 생식선 차폐기구를 사용하지 않았을 경우에서 $9.5{\pm}0.3$ mGy, 대($650m^2$)크기의 차폐체를 사용하였을 경우 $2.8{\pm}0.2$ mGy로 나타나 70% 의 생식선 피폭선량 감소효과를 보였다. MDCT를 이용한 검사 시 생식선 차폐기구 사용 유, 무에 따라 영상을 획득하여 Likert 5점 척도로 분석한 결과 복부 영상에서는 4.1점으로 동일한 결과를 보였다. 또한 골반부 검사에서는 생식선 차폐기구 사용하였을 때 1.2점, 생식선 차폐기구 사용하지 않았을 때 4.1점으로 나타났다. 이상의 결과에서 복부 128-MDCT 검사 시 생식선 차폐기구를 사용하여 영상의 질 저하 없이 영상을 획득하고 피폭선량을 감소하여야 할 것으로 사료된다.

• Animal cells and systems
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• 제5권1호
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• pp.37-44
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• 2001

Gonadal development, reproductive cycle, gonad index, meat weight rate, and first sexual maturity of the top shell, Omphalius rusticus were Investigated monthly by histological observations. Specimens were collected from the west coast of Korea during the period from January to December in 1999. O. rusticus is dioecious and oviparous. The gonad is widely situated on the surface of the digestive g1and located in the posterior spiral meat part in the shell. The ovary and the testis were composed of a number of oogenic follicles and several spermatogenic follicles, respectively. Ripe oocytes were approximately 120-130 $\mu$m in diameter. The meat weight rate peaked in June (27.7%), and then rapidly decreased in September (19.5%). Monthly changes in the gonad index in both sexes reached the maximum in June, and then sharply decreased in September. Percentages of first sexual maturity of female and male snails ranging from 9.0 to 9.9 mm in shell heights were 58.3% and 54.5%, respectively, and 100% in those over 11.0 mm in both sexes participated in reproduction. Reproductive cycle of this species can be categorized into five successive stages: in females, early active (October to April), late active (December to June), ripe (April to September), spawning (July to September) and recovery (September to January): in males, early active (November to March), late active (December to June), ripe (April to September), spawning (July to September) and recovery (September to December). Gonadal development, gametogenesis, reproductive cycle, and spawning were closely related to the seawater temperature.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.740-744
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• 2002

The relationship of gonad weight and liver weight with body weight of six fish species viz; Catla catla, Labeo rohita, Cirrhina mrigala, Hypophthlamichthys molitrix, Ctenopharyngodon idella and Cyprinus carpio under the influence of artificial feed, broiler manure, buffalo manure, N:P:K (25:25:0) and a control pond were examined after a rearing period of one year. The positive relationship between gonad weight and body weight was significant which showed the dependence of gonadal development on body weight in all the six fish species. The currelation coefficients were higher in female fishes. However, the major carps had a much smaller proportional gonadal weight as compared with Chinese carps and a common carp due to their faster growth rate. The overall comparison of six fish species under different experimental treatments revealed that highest liver weight was observed for C. idella closely followed by C. carpio. The maximum correlation value was observed with H. molitrix under the broiler manure. The maximum Gonadosomatic Index (GSI) remained as 32.63 for C. carpio followed by C. idella. The maximum value for Hepatosomatic Index (HSI). remained 1.99 for C. idella followed by C. mrigala.