• 한국약용작물학회지
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• 제27권1호
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• pp.9-16
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• 2019

Background: Various Schisandra chinensis (SC) varieties grow in diverse regions in Korea. However, there is no valid scientific evidence of these varieties. This study aimed to select the excellent resources in terms of the growth characteristics, antioxidant activities, and analysis of the active compounds of the SC collection. Method and Results: In total, 154 resources of SC were collected from various regions of Korea. The growth characteristics were measured by the number of fruit bunches, fruit number, and weight of 100 fruits. The antioxidant activities were investigated by analyzing the total flavonoid and total polyphenol contents and the radical scavenging activity of DPPH and ABTS. Schizandrin A, schizandrin c, gomisin a, and gomisin N were analyzed by HPLC. Each resource showed different growth characteristics. Among the antioxidative effects, the highest 20 resources showed high antioxidant activities in selected 29 resources. Analysis of the SC lignan index showed that all resources contained more than 1.16% of active compounds. Conclusions: All of the selected 29 SC resources were shown to have excellent growth characteristics, antioxidant activities, and bioactive compound richness. Especially, SC-004, SC-007, and SC-154 showed the best growth characteristics, and SC-22, SC-40, and SC-45 showed the best antioxidant activities and bioactive compound richness.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.101-101
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• 2022

Schisandra chinensis is a fruit which is called Omija in South Korea. The Korean word Omija means "five flavors" (sweet, spicy, sour, bitter, and salty). The aim of his study was to investigate the correlation between growth characteristics and lignans (gomisin A, gomisin N, schisandrin) contents of Schisandra chinensis. The method for determining lignans was validated by measuring the linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy using UPLC-UV. Growth characteristics of S. chinensis such as number of fruits per fruit bunch, length of fruit bunch, width of fruit bunch, fresh weight of fruit bunch, length of fruit, width of fruit, fresh weight of fruit, fresh weight of 30 fruits, and sugar contents of fruit were measured. From the results of correlation analysis, it was found that the contents of lignans showed a significantly negative correlation with fresh weight and sugar contents of fruit. These results will be used to study for quality control of S. chinensis fruit.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.269-270
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• 2009

• Nutrition Research and Practice
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• 제12권4호
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• pp.291-297
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• 2018

BACKGROUND/OBJECTIVES: This study evaluated the effects and molecular mechanisms of the Schisandra chinensis fruit extract (SC) and its major compound gomisin A (GA), on the contractility of rabbit penile corpus cavernosum smooth muscle (PCCSM). MATERIALS/METHODS: PCCSM was exposed to SC or GA after appropriate pretreatment with nitric oxide synthase (NOS) blocker, guanylate cyclase blocker, adenylyl cyclase blocker or protein kinase A blocker. Subsequently, we evaluated the cyclic nucleotide in the perfusate by radioimmunoassay, protein expression level of neuronal NOS (nNOS) and endothelial NOS (eNOS) by western blot, and the interaction of SC or GA with udenafil and rolipram. RESULTS: Both SC and GA induce PCCSM relaxations in a concentration-dependent manner. Pretreatment with NOS blocker, guanylate cyclase blocker, adenylyl cyclase blocker or protein kinase A blocker result in significantly decreased relaxation. SC and GA also induce the levels of cyclic nucleotide in the perfusate in a concentration-dependent manner. Perfusion with GA also showed significantly higher levels of eNOS protein. Furthermore, the udenafil and rolipram induced relaxations of PCCSM were enhanced after exposure to SC and GA. Our results indicate that SC and GA induce the relaxation of PCCSM via the nitric oxide (NO)-cGMP and cAMP signaling pathways. CONCLUSIONS: The SC and GA are potential alternative treatments for men who want to consume natural products to ameliorate erectile function, or who do not respond to the commercially available medicines.

• 한국식품과학회지
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• 제33권3호
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• pp.389-394
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• 2001

오미자 열매로부터 항균활성물질을 분리하기 위하여 methanol 조추출물의 용매분획의 항균력을 측정하였다. 항균활성을 나타낸 methanol 조추출물은 다시 여러 가지 용매로 순차적으로 분배하여 n-hexan층, dichloromethane층, ethyl acetate층, buthanol층과 water층을 얻은 다음, 이들 분배층에 대해 항균활성을 검색한 결과, ethyl acetate층에서 가장 활성이 높게 나타났다. 활성이 높게 나타난 ethyl acetate층은 감압증류한 후, 여러 가지 chromatography를 이용하여 분리, 정제한 다음, MS, $^1H-NMR,\;^{13}C-NMR,\;$, DEPT 및 2D NMR등을 이용하여 항균활성물질의 구조를 동정하였다. 그 결과 오미자의 약리기능을 나타내는 주요성분인 lignan계통의 화합물인 gomisin C로 추정되는 compound를 분리하였고, 다른 하나는 오미자의 정유성분인 trimethylcitrate로 동정하였다. 또한 오미자추출물의 균증식도와 분쇄육의 저장성에 미치는 효과를 검토하기 위하여 식품보장실험을 수행하였다. 그 결과 Salmonella typhimurium에 의해 문제시되는 식품에 천연보존료로서의 이용가능성을 시사하였다.

• Biomolecules & Therapeutics
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• 제17권1호
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• pp.47-56
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• 2009

Schizandra chinensis (S. chinensis) exhibits a harmless, 'adaptogen-type' effect leading to improvements in mental performance and learning efficacy in brain. Activated microglia contributes to neuronal injury by releasing neurotoxic products, which make it important to regulate microglial activation to prevent further cytological as well as functional brain damage. However, the effect of S. chinensis on microglial activation has not been examined yet. We have investigated the effects of four compounds (Gomisin A, Gomisin N, Schizandrin and Schizandrol A) from S. chinensis on lipopolysaccharide (LPS)-induced microglial activation. In this study, BV2 microglial cells were activated with LPS and the microglial activation was assessed by up-regulation of activation markers such as nitric oxide (NO), reactive oxygen species (ROS), and matrix metalloproteinase-9 (MMP-9). The results showed that all four compounds significantly reduced the intracellular level of ROS, the release of NO and MMP-9 as well as LPS-induced phosphorylation of ERK1/2. These results strongly suggested that S. chinensis may be useful to modulate inflammation-mediated brain damage by regulating microglial activation.

• 한국식품영양과학회지
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• 제45권6호
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• pp.851-858
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• 2016

오미자 열매를 과피, 과육 및 씨 등을 부위별로 분리한 후 일반성분을 분석한 결과 모든 부분에서 가용성 무질소물의 함량이 각각 20.72, 6.88 및 24.42%로 가장 많이 함유되어 있으며 열매에서 씨 부분이 차지하는 비율은 40% 이상이었다. 또한, 오미자의 유효성분인 schizandrin을 포함한 리그 난 화합물의 총량이 씨 부분에서 15.55 mg/g으로 가장 많이 함유되어 있고 schizandrin이 5.68 mg/g, gomisin N이 2.90 mg/g, gomisin A 1.55 mg/g, schizandrin C가 1.53 mg/g 함유되어 있었으며 열풍 건조한 오미자 열매 전과와 거의 비슷한 함량을 함유하고 있었다. 오미자의 유기산은 주로 과피 쪽에 있었으며 총유기산 함량은 12.99%를 함유하고 있었고 주된 유기산은 citric acid로 4.84%를 함유하고 있었다. 오미자의 지방 함량은 씨 부위 및 열풍 건조한 오미자 열매에서 각각 19.04%와 15.11%를 함유하고 있었고 지방을 구성하고 있는 주된 지방산은 linoleic acid로 각각 72.66%와 74.10%를 차지하고 있었으며, 그다음은 oleic acid로 각각 14.78%와 17.46%를 차지하고 있었다. 오미자의 주된 무기이온은 씨에서는 P로 725.35 mg/100 g을, 열풍 건조한 열매 전과에서는 K로 2,024.10 mg/100 g을 차지하고 있었다. 결론적으로 오미자를 활용하기 위해서는 리그 난 화합물이 많이 함유되어 있는 씨 부분을 충분히 활용할 수 있는 방법을 고려하여야 할 것으로 생각된다.