• 한국발생생물학회지:발생과생식
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• 제13권3호
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• pp.191-198
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• 2009

In an attempt to simultaneously produce two human proteins, hGH and hG-CSF, in the milk of transgenic mice, we constructed goat $\beta$-casein-directed hGH and hG-CSF expression cassettes individually and generated transgenic mice by co-injecting them into mouse zygotes. Out of 33 transgenic mice, 29 were identified as double transgenic harboring both transgenes on their genome. All analyzed double transgenic females secreted both hGH and hG-CSF in their milks. Concentrations ranged from 2.1 to $12.4\;mg/m{\ell}$ for hGH and from 0.04 to $0.13\;mg/m{\ell}$ for hG-CSF. hG-CSF level was much lower than hGH level but very similar to that of single hG-CSF mice, which were introduced with hG-CSF cassette alone. In order to address the causes of concentration difference between hGH and hG-CSF in milk, we examined mRNA level of hGH and hG-CSF in the mammary glands of double transgenic mice and tissue specificity of hG-CSF mRNA expression in both double and single transgenic mice. Likewise protein levels in milk, hGH mRNA level was much higher than hG-CSF mRNA, and hG-CSF mRNA expression was definitely specific to the mammary glands of both double and single transgenic mice. These results demonstrated that two transgenes have distinct transcriptional potentials without interaction each other in double transgenic mice although two transgenes co-integrated into same genomic sites and their expressions were directed by the same goat $\beta$-casein promoter. Therefore goat $\beta$-casein promoter is very useful for the multiple production of human proteins in the milk of transgenic animals.

• 한국축산식품학회지
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• 제28권2호
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• pp.218-221
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• 2008

본 연구는 최근 웰빙식품으로 소비가 증가하고 있는 산양유의 체세포수 등급제정을 위해 (주)엠젠에 납유하고 있는 12개 목장에서 2006년 12월부터 2007년 11월까지 납유한 산양유의 체세포수를 분석하였다. 프랑스와 노르웨이의 체세포 등급체계 및 우리나라 원유의 체세포 등급체계를 고려할 때, 산양유의 체세포수 등급체계는 1급이 mL당 100만 미만, 2급이 100-150만 미만, 3급이 150-200만 미만, 4급이 200-250만 미만, 5급이 250만 초과로 규정하는 것이 바람직할 것으로 생각된다. 이러한 체계를 가정할 때 우리나라 산양유의 1등급 비율이 26.2%로 다소 낮고, 5등급 비율이 11.8%로 다소 높으나 이를 통해 산양유의 체세포수가 개선될 것으로 기대된다.

• Journal of Dairy Science and Biotechnology
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• 제35권1호
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• pp.33-38
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• 2017

본 연구는 상업유산균인 단일 균주와 복합 균주를 이용한 산양유의 발효형태와 저장 중 풍미변화를 조사한 결과가 있다. 복합 균주는 단일 균주보다 단시간에 있어서 낮은 pH와 높은 산도를 나타내었다. 또한 복합 균주에서는 유산균 수의 증식 속도도 빠르게 나타내었다. 관능검사는 패널의 숙련도에 따라 느끼는 맛이 다르다. 따라서 수많은 시료를 분석할 경우, 재현성이 있는 객관적인 데이터를 얻기 어려운 단점이 있다. 더욱이 우리는 미각센서를 이용하여 저장 중 풍미변화를 측정하였다. 미각센서는 단일 균주와 복합 균주를 이용한 산양발효유에 대하여 사용 균주별 다양한 풍미의 변화가 측정 가능하였다. 따라서 본 연구는 국내에서 처음으로 발효유에 있어서 미각센서를 이용하여 저온 저장기간 동안 맛 성분의 변화를 측정한 자료로 있다. 이러한 결과는 차후 유제품의 소비기한 유지에 대한 자료로서 이용가치가 크게 기대되고 있다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1513-1513
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• 2001

Present Food Legislation compels dairy industry to carry out analyses in order to guarantee the food safety and quality of products. Furthermore, in many cases industry pays milk according to bacteriological or/and nutritional quality. In order to do these analyses, several expensive instruments are needed (Milkoscan, Fossomatic, Bactoscan). NIRS technology Provides a unique instrument to deal with all analytical requirements. It offers as main advantages its speed and, specially, its versatility, since not only allows determine all the parameters required in milk analysis, but also allows analyse other dairy products, like cheese or whey. The objective of this study is to develop NIRS calibration equations to predict several quality parameters in goat milk, cheese and whey. Three sets of 123 milk samples, 190 cheese samples and 109 whey samples, have been analysed in a FOSS NIR Systems 6500 I spectrophotometer equipped with a spinning module. Milk and whey were analysed by folded transmission, using circular cells with gold surface and pathlength of 0.1 m, while intact cheese was analysed by reflectance using standard circular cells. NIRS calibrations were obtained for the prediction of chemical composition in goat milk, for fat (r$^2$=0.92; SECV=0.20%), total solids (r$^2$=0.95: SECV=0.22%), protein (r$^2$=0.94; SECV=0.07%), casein (r$^2$=0.93; SECV=0.07%) and lactose (r$^2$=0.89; SECV=0.05%). Moreover, equations have been performed to determine somatic cells (r$^2$=0.81; SECV=276.89%) and total bacteria (r$^2$=0.58; SECV=499.32%) counts in goat milk. In the case of cheese, calibrations were obtained for the prediction of fat (r$^2$=0.92; SECV=0.57), total solids (r$^2$=0.80; SECV=0.92%) and protein (r$^2$=0.70; SECV=0.63%). In whey, fat (r$^2$=0.66; SECV=0.08%), total solids (r$^2$=0.67; SECV=0.19%) and protein (r$^2$=0.76; SECV=0.07%) NIRS equations were obtained. These results proved the viability of NIRS technology to predict chemical and microbiological parameters and somatic cells count in goat milk, as well as chemical composition of goat cheese and whey.

• Asian-Australasian Journal of Animal Sciences
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• 제29권7호
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• pp.1022-1028
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• 2016

Milk composition is an imperative aspect which influences the quality of dairy products. The objective of study was to compare the chemical composition, nitrogen fractions and amino acids profile of milk from buffalo, cow, sheep, goat, and camel. Sheep milk was found to be highest in fat ($6.82%{\pm}0.04%$), solid-not-fat ($11.24%{\pm}0.02%$), total solids ($18.05%{\pm}0.05%$), protein ($5.15%{\pm}0.06%$) and casein ($3.87%{\pm}0.04%$) contents followed by buffalo milk. Maximum whey proteins were observed in camel milk ($0.80%{\pm}0.03%$), buffalo ($0.68%{\pm}0.02%$) and sheep ($0.66%{\pm}0.02%$) milk. The non-protein-nitrogen contents varied from 0.33% to 0.62% among different milk species. The highest r-values were recorded for correlations between crude protein and casein in buffalo (r = 0.82), cow (r = 0.88), sheep (r = 0.86) and goat milk (r = 0.98). The caseins and whey proteins were also positively correlated with true proteins in all milk species. A favorable balance of branched-chain amino acids; leucine, isoleucine, and valine were found both in casein and whey proteins. Leucine content was highest in cow ($108{\pm}2.3mg/g$), camel ($96{\pm}2.2mg/g$) and buffalo ($90{\pm}2.4mg/g$) milk caseins. Maximum concentrations of isoleucine, phenylalanine, and histidine were noticed in goat milk caseins. Glutamic acid and proline were dominant among non-essential amino acids. Conclusively, current exploration is important for milk processors to design nutritious and consistent quality end products.

• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1503-1507
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• 2006

Goats' milk adulteration with cows' milk is becoming a big problem. In the past, the urea-polyacrylamide gel electrophoresis assay with different motility of ${\alpha}S1$-casein has been applied for the identification of cows' milk adulteration. The detection sensitivity is 1.0%. The aim of this study was to develop a faster and more sensitive method to detect cows' milk which may be present in adulterated goats' milk and goats' milk powder. The published primer was targeted at highly conserved regions in bovine mitochondrial DNA (a 271 bp amplicon). This amplicon was cloned and sequenced to further confirm bovine specific sequence. The chelex-100 was used to separate bovine somatic cells from goats' milk or goats' milk powder samples. Random sampling of different brands of goats' milk powder and tablets from various regions of Taiwan showed the adulterated rate was 20 out of 80 (25%) in goats' milk powders and 12 out of 24 (50%) in goats' milk tablets. With this system, as low as 0.1% cows' milk or cows' milk powder in goat milk or goat milk powder could be identified. This chelex DNA isolation approach provides a fast, highly reproducible and sensitive method for detecting the adulteration of goats' milk products.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.555-559
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• 1993

Studies at freezing point depression (FPD) for goat milk are described in this paper. The 432 samples of raw goat milk collected in a period of two years and analysed by the use of an electronic cryoscope gave a mean value of FPD: $-0.565{\pm}0.01^{\circ}C$. Statistical significant differences were found between the morning and evening milk. The evening milk showed better quality considering the lactose and others dissolved solutes. Among the Brown, Saanen and Anglo-nubian goats, the greatest increase in FPD, occurred in the Anglo-nubian breed. The statistical analysis revealed a significant breed difference (p<0.05), when climate variation was observed. The superior and inferior values found to the FPD of the caprine milk (-0.545 to $-0.585^{\circ}C$), is important in establishing regional rules, to improve the quality control criteria and legal interpretation of standards found in the pertinent legislation. The results are also important to improve herd management so that one can increase the animal production which will affect the technological composition, and the nutritional characteristics of the resultant products.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.45-54
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• 2002

Ninety lactating goats (Bachthao, Barbary, Beetal and Jamnapary breeds) were used in an experiment to investigate the replacement value of the tree fodders Flemingia (Flemingia macrophylla) and Jackfruit (Artocarpus heterophyllus). The foliages were used to replace the concentrate in diets based on chopped whole sugar cane (Sacharatum sp.), Para grass (Brachiaria mutica) and dried cassava root (Manihot esculanta). The concentrate was replaced by foliage of Jackfruit or Flemingia at 0%, 20%, 40%, 60% and 80% based on the crude protein (CP) content in the concentrate and foliages, respectively. Average milk yield was 1,617 g/day for goats fed Jackfruit compared to 1,532 g/day for those fed Flemingia. Increasing amounts of Flemingia foliage resulted in reduced dry matter intake and decreased milk yield but milk composition (CP, casein and fat content) was similar up to 60% replacement. Flemingia showed a poor potential as a supplement for lactating goats and replacement levels should not exceed 20% of the protein in the concentrate or 7.5% of the dry matter in the diet. With respect to the combination of milk production and net return over the control a CP replacement rate of 20% was the most promising. For Jackfruit there was similar feed intake and milk yield at a replacement level of 20% of CP in the concentrate (9.2% of DM intake) compared to the control diet. Milk yield at the level of 40% replacement (15% of the DM) in the diet was slightly reduced. Up to a level of CP replacement rate of 60% (21% DM in the diet) can be suggested for on-farm testing as a higher net return over the control was obtained on station.

• Nutrition Research and Practice
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• 제5권4호
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• pp.308-312
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• 2011

This was a prospective cohort study of 976 infants from birth to 12 months of age. Infants were fed breast milk, goat infant formula, cow infant formula, or a combination of formula and breast milk during the first 4 months of age. Data on type of milk feeding and infant growth (weight and height) were collected at birth and at 4, 8, and 12 months during routine clinical assessment. The number and consistency of bowel motions per day were recorded based on observational data supplied by the mothers. Infants fed breast milk or goat or cow infant formula during the first 4 months displayed similar growth outcomes. More of the infants fed cow infant formula had fewer and more well-formed bowel motions compared with breast-fed infants. The stool characteristics of infants fed goat formula resembled those of infants fed breast milk.

• Journal of Dairy Science and Biotechnology
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• 제33권2호
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• pp.103-110
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• 2015

산양유을 섭취한 성인의 분변 내 bifidobacteria의 증식 효과 및 분변에서 분리된 bifidobacteria와 lactobacilli의 당 발효 능력을 조사하였다. 1. 산양유를 섭취한 처리군이 대조군보다 bifidobacteria 세균수가 상대적으로 높았으며, 8주에서 95% 신뢰한계에서 유의성이 있었다. 2. 분변에서 분리한 균주들을 13 균주의 16S rRNA 염기서 열로 동정한 결과, 산양유를 섭취한 처리구는 B. adolescentis, B. longum, B. pseudocatenulatum, B. dentium, L. sakei 순으로 분리되었다. 대조군의 12 균주는 B. adolescentis, B. longum, L. ruminis, L. sakei, B. pseudocatenulatum 순으로 분리되었다. 3. 산양유 올리고당과 lactulose는 모든 bifidobacteria 및 lactobacilli 균주가 발효하였다. Fructooligosaccaride는 B. adolescentis 7 균주 모두 발효하였다. 조사한 B. pseudocatenulatum 4 균주, L. sakei 3 균주, B. longum 7 균주 중에서 각각 3, 2, 1 균주가 fructooligosaccharide를 발효하였다.