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http://dx.doi.org/10.5713/ajas.15.0452

Chemical Composition, Nitrogen Fractions and Amino Acids Profile of Milk from Different Animal Species  

Rafiq, Saima (National Institute of Food Science and Technology, University of Agriculture)
Huma, Nuzhat (National Institute of Food Science and Technology, University of Agriculture)
Pasha, Imran (National Institute of Food Science and Technology, University of Agriculture)
Sameen, Aysha (National Institute of Food Science and Technology, University of Agriculture)
Mukhtar, Omer (Food and Marine Resources Research Center, Pakistan Council for Scientific and Industrial Research)
Khan, Muhammad Issa (National Institute of Food Science and Technology, University of Agriculture)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.29, no.7, 2016 , pp. 1022-1028 More about this Journal
Abstract
Milk composition is an imperative aspect which influences the quality of dairy products. The objective of study was to compare the chemical composition, nitrogen fractions and amino acids profile of milk from buffalo, cow, sheep, goat, and camel. Sheep milk was found to be highest in fat ($6.82%{\pm}0.04%$), solid-not-fat ($11.24%{\pm}0.02%$), total solids ($18.05%{\pm}0.05%$), protein ($5.15%{\pm}0.06%$) and casein ($3.87%{\pm}0.04%$) contents followed by buffalo milk. Maximum whey proteins were observed in camel milk ($0.80%{\pm}0.03%$), buffalo ($0.68%{\pm}0.02%$) and sheep ($0.66%{\pm}0.02%$) milk. The non-protein-nitrogen contents varied from 0.33% to 0.62% among different milk species. The highest r-values were recorded for correlations between crude protein and casein in buffalo (r = 0.82), cow (r = 0.88), sheep (r = 0.86) and goat milk (r = 0.98). The caseins and whey proteins were also positively correlated with true proteins in all milk species. A favorable balance of branched-chain amino acids; leucine, isoleucine, and valine were found both in casein and whey proteins. Leucine content was highest in cow ($108{\pm}2.3mg/g$), camel ($96{\pm}2.2mg/g$) and buffalo ($90{\pm}2.4mg/g$) milk caseins. Maximum concentrations of isoleucine, phenylalanine, and histidine were noticed in goat milk caseins. Glutamic acid and proline were dominant among non-essential amino acids. Conclusively, current exploration is important for milk processors to design nutritious and consistent quality end products.
Keywords
Milk Species; Milk Composition; Casein; Whey Protein; Amino Acids;
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1 Ahmad, S., I. Gaucher, F. Rousseau, E. Beaucher, M. Piot, J. F. Grongnet, and F. Gaucheron. 2008. Effects of acidification on physico-chemical characteristics of buffalo milk: A comparison with cow's milk. Food Chem. 106:11-17.   DOI
2 Al Haj, O. A. and H. A. Al Kanhal. 2010. Compositional, technological and nutritional aspects of dromedary camel milk. Int. Dairy J. 20:811-821.   DOI
3 Anthony, J. C., T. G. Anthony, S. R. Kimball, and L. S. Jefferson. 2001. Signaling pathways involved in translational control of protein synthesis in skeletal muscle by leucine. J. Nutr. 131:856-860.   DOI
4 AOAC. 2005. Official Methods of Analysis of AOAC International 18th edn., Association of Official Analytical Chemists, Gaithersburg, MD, USA.
5 Bernabucci, U. N., N. Lacetera, B. Ronchi, and A. Nardone. 2002. Effects of the hot season on milk protein fractions in Holstein cows. Anim. Res. 51:25-33.   DOI
6 Borkova, M. and J. Snaselova. 2005. Possibilities of different milk detection in milk and dairy products-A review. Czech J. Food Sci. 23:41-50.
7 Bos, C., C. Gaudichon, and D. Tome. 2000. Nutritional and physiological criteria in the assessment of milk protein quality for humans. J. Am. Coll. Nutr. 19:191-205.   DOI
8 David, P. 1976. The chemical analysis of foods. 7th edn. Churchill Livingston, London, UK.
9 Fouillet, H., F. Mariotti, C. Gaudichon, C. Bos, and D. Tome. 2002. Peripheral and splanchnic metabolism of dietary nitrogen are differently affected by the protein source in humans as assessed by compartmental modeling. J. Nutr. 132:125-133.   DOI
10 Fox, P. F., T. P. Guinee, T. M. Cogan, and P. L. H. McSweeney. 2000. Fundamentals of Cheese Science. Aspen Publishers, Inc. Gaithersburg, MD, USA.
11 GOP (Government of Pakistan). 2014-15. Pakistan Economic Survey. Economic Advisor's Wing, Finance Division, Islamabad, Pakistan.
12 Ha, E. and M. B. Zemel. 2003. Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people. J. Nutr. Biochem. 14:251-258.   DOI
13 Imran, M., H. Khan, S. S. Hassan, and R. Khan. 2008. Physicochemical characteristics of various milk samples available in Pakistan. J. Zhejiang Univ. Sci. 9:546-551.
14 Hall, W. L., D. J. Millward, S. J. Long, and L. M. Morgan. 2003. Casein and whey exert different effects on plasma amino acid profiles, gastrointestinal hormone secretion and appetite. Br. J. Nutr. 89:239-248.   DOI
15 Han, B. Z., Y. Meng, M. Li, Y. X. Yang, F. Z. Ren, Q. K. Zeng, and M. J. Robert Nout. 2007. A survey on the microbiological and chemical composition of buffalo milk in China. Food Control 18:742-746.   DOI
16 IDF (International Dairy Federation). 1993. Determination of milk nitrogen content. 4. Determination of non-protein nitrogen. International Standard FIL-IDF 20B.
17 Khaskheli, M., M. A. Arain, S. Chaudhry, A. H. Soomro, and T. A. Qureshi. 2005. Physico-chemical quality of camel milk. J. Agric. Soc. Sci. 1:164-166.
18 Kittivachra, R. R., R. Sanguandeekul R. Sakulbumrungsil, and P. Phongphanphanee. 2007. Factors affecting lactose quantity in raw milk. Songklanakarin J. Sci. Technol. 29:937-943.
19 Mal, G., S. D. Suchitra, and M. S. Sahani. 2007. Changes in chemical and macro-minerals content of dromedary milk during lactation. J. Camel Pract. Res. 14:195-197.
20 Marshall, R. T. 1993. Standard methods for the examination of dairy products 16th edn. American Publication Health Association (APHA), Washington, DC, USA.
21 Ozrenk, E. and S. S. Inci. 2008. The effect of seasonal variation on the composition of cow milk in Van Province. Pakistan J. Nutr. 7:161-164.   DOI
22 Schaafsma, G. 2000. The protein digestibility-corrected amino acid score. J. Nutr. 130:1865-1867.   DOI
23 Pavic, V., N. Antunac, B. Mioc, A. Ivankovic, and J. L. Havranek. 2002. Influence of stage of lactation on the chemical composition and physical properties of sheep milk. Czech J. Anim. Sci. 47:80-84.
24 Pirsi, A., G. Piredda, M. Corona, M. Pes, S. Pintus, and A. Ledda. 2000. Influence of somatic cell count in ewe's milk composition, cheese yield and cheese quality. In: Proceeding of the 6th great lakes, Dairy Sheep Symposium, Guelph, Canada. pp. 47-59.
25 Salem, S. A., E. I. El-Agamy, F. A. Salama, and N. H. Abu-Soliman. 2009. Isolation, molecular and biochemical characterization of goat milk casein and its fractions. Trop. Subtrop. Agroecosyst. 11:29-35.
26 Schuster, R. 1988. Determination of amino acids in biological, pharmaceutical, plant and food samples by automated precolumn derivatization and high-performance liquid chromatography. J. Chromatogr. 431:271-284.   DOI
27 Shamsia, S. M. 2009. Nutritional and therapeutic properties of camel and human milks. Int. J. Genet. Mol. Biol. 1:52-58.
28 Stancheva, N. N. Naydenova, and G. Staikova. 2011. Physicochemical composition, properties, and technological characteristics of sheep milk from the Bulgarian dairy synthetic population. Macedonian J. Anim. Sci. 1:73-76.
29 Steel, R. G. D., J. H. Torrie, and D. A. Dickey. 1997. Principles and Procedures of Statistics. A Bio-Metrical Approach 3rd Ed. McGraw Hill Book Co. Inc., New York, NY, USA.
30 Strzalkowska, N., A. Jozwik, E. Bagnicka, J. Krzyzewski, K. Horbanczuk, B. Pyzel, and J. O. Horbanczuk. 2009. Chemical composition, physical traits and fatty acid profile of goat milk as related to the stage of lactation. Anim. Sci. Pap. Rep. 27:311-320.
31 Uallah, S., T. Ahmad, M. Q. Bilal, Zia-ur-Rahman, and G. Muhammad. 2005. The effect of severity of mastitis on protein and fat contents of buffalo milk. Pak. Vet. J. 25:1-4.