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Rheological, Physical and Sensory Evaluation of Low-Fat Cupuassu Goat Milk Yogurts Supplemented with Fat Replacer

  • Costa, Marion P.;Rosario, Anisio Iuri L.S.;Silva, Vitor L.M.;Vieira, Carla P.;Conte-Junior, Carlos A.
    • Food Science of Animal Resources
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    • v.42 no.2
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    • pp.210-224
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    • 2022
  • The use of skim milk is a strategy to increase goat milk yogurt acceptability. However, it can negatively affect yogurt rheology because fat plays a vital role in dairy structural integrity. Thus, this study aimed to investigate the effects of fat replacers on the rheological, physical, and sensory parameters of low-fat cupuassu goat milk yogurts during refrigerated storage (28 days). Five goat milk yogurts formulations were carried out: whole yogurt (WY), skim yogurt (SY), skim yogurt with inulin (SIY), skim yogurt with maltodextrin (SMY), and skim yogurt with whey protein (SWY). Treatments were subjected to bacterial counts, chemical composition, pH, water holding capacity, instrumental color and texture, rheological and sensory analyses. All samples showed reducing pH values, water holding capacity, and L* and b* value during storage. Regarding texture, the firmness and consistency decreased during storage. On the other hand, the viscosity index significantly increased during refrigerated storage time. Moreover, all treatments exhibited viscoelastic behaviour. In addition, SIY and SMY showed the highest apparent viscosity. Furthermore, SIY, SMY, and SWY formulations exhibited positive sensory scores for appearance, color, aroma, texture, and viscosity. However, the overall acceptability and purchase intention did not differ statistically between WY and the fat-replacement treatments (SIY, SMY, and SWY). These results indicate that fat substitutes improved the quality of skimmed formulations. Thus, inulin and maltodextrin have the potential as functional fat replaces to produce low-fat goat milk yogurts.

Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

  • Zhu, Wen;Liu, Tianwei;Deng, Jian;Wei, Cong Cong;Zhang, Zi Jun;Wang, Di Ming;Chen, Xing Yong
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1535-1544
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    • 2022
  • Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

Quality Characteristics of Goat Meat Tteokgalbi Prepared with the Addition of Centella asiatica Powder (병풀분말을 첨가한 염소고기 떡갈비의 품질특성)

  • Jeong Ah Lee;Soo-Hyun Cho;Hyoun-Wook Kim;Yunseok Kim;In-Seon Bae
    • Journal of the Korean Society of Food Culture
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    • v.38 no.5
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    • pp.336-344
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    • 2023
  • This study aimed to investigate the effects of Centella asiatica powder on the quality characteristics and storage stability of goat meat Tteokgalbi. The goat meat Tteokgalbi samples were prepared with four different quantities (0, 0.5, 1, and 1.5%) of Centella asiatica powder. The moisture content of the samples containing Centella asiatica powder was significantly lower than that of the control (p<0.05). The protein content of the samples containing Centella asiatica powder was significantly higher than that of the control (p<0.05). As the proportion of Centella asiatica powder increased, the pH, L*, and a* values of the uncooked and cooked samples decreased (p<0.05). The water holding capacity and the cooking yield of the uncooked and cooked samples were higher in the samples containing Centella asiatica powder than in the control (p<0.05). Also, with the increasing amounts of the Centella asiatica powder, the thiobarbituric acid reactive substance (TBARS) levels decreased. The 1-1.5% Centella asiatica powder group showed lower levels of volatile basic nitrogen (VBN) than the other groups as per the assay (p<0.05). The results indicate that Centella asiatica powder could enhance the quality characteristics and storage stability of goat meat Tteokgabi.

Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature

  • Wenzheng Shen;Yu Fu;Haiyu Bai;Zhiyu Zhang;Zhikun Cao;Zibo Liu;Chao Yang;Shixin Sun;Lei Wang;Chunhuan Ren;Yinghui Ling;Zijun Zhang;Hongguo Cao
    • Animal Bioscience
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    • v.37 no.4
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    • pp.640-654
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    • 2024
  • Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.

Physicochemical Properties of Restructured Black Goat Jerky with Various Types of Ultra-Ground Seaweed Powders

  • Ui-Bin Baek;Hack-Youn Kim
    • Food Science of Animal Resources
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    • v.44 no.2
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    • pp.483-497
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    • 2024
  • This study investigated the effects of ultra-ground seaweed powders (USP) on the physicochemical properties (proximate composition, mineral contents, pH, color, shear force, sensory evaluation, electronic nose, and electronic tongue) of restructured black goat jerky. Restructured black goat jerky was prepared using three different treatments, i.e., 3% (w/w) each of ultra-ground sea tangle (ST; Undaria pinnatifida), sea mustard (SM; Saccharina japonica), and sea string (SS; Gracilaria verrucosa) powders. Moisture and ash contents were significantly higher in the USP-treated group than in the control (p<0.05). Potassium, calcium, and zinc contents were significantly higher in the SM than in the other USP-treated groups (p<0.05). In contrast, pH values were significantly higher in the ST and SM than in the control and SS (p<0.05). CIE L*, CIE a*, CIE b*, and shear force were significantly lower in the USP-treated groups than in the control (p<0.05). Sensory evaluation revealed no significant difference in taste, texture, seaweed-like odor, and goaty flavor (p<0.05). Principal component analysis (PCA) and peak graph analysis of the electronic nose showed that the SS differed the most from the control compared with the other USP-treated groups, owing to the seaweed odor of ultra-ground SS powder. The PCA and ranking analysis of the electronic tongue showed that the umami taste of the SM was higher than that of the control and other USP-treated groups. Therefore, the potassium, calcium, zinc contents, and umami taste of reconstituted black goat jerky were significantly higher in the SM than in the control and other USP-treated groups.

Characteristics of β-casein Gene using the PCR Technique in Korean Native Goat (PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성)

  • Kim, Ji-Ae;Ryoo, Seung-Heui;Yu, Sung-Lan;Lee, Jun-Heon;Seo, Gil-Woong;Kim, Sun-Kyun;Sang, Byung-Chan
    • Korean Journal of Agricultural Science
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    • v.29 no.2
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    • pp.43-52
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    • 2002
  • This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of $\beta$-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of $\beta$-casein loci by PCR. The $\beta$-casein AB genotype showed 481, 284 and 197bp, and $\beta$-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of $\beta$-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of $\beta$-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of $\beta$-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of $\beta$-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of $\beta$-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for $\beta$-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.

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IMMUNOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDIES ON TWO GASTRIC ENZYMES IN NEONATE, YOUNG AND ADULT GOATS

  • Amasaki, H.;Gozawa, S.;Akuzawa, R.;Suzuki, K.;Daigo, M.;Andren, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.4
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    • pp.281-285
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    • 1990
  • The present paper demonstrates the expressions and amounts of pepsinogen and prochymosin in neonate, young and adult goat's proper gastric glandular regions by the immunochemical and the immunohistochemical analyses with the anti-bovine pepsinogen serum and anti-bovine chymosin serum. Each bovine serum was demonstrated to have reactivities against corresponding goat's antigen by immunochemical analses and enzymatic activities. The anti-pepsinogen was higher in the new born animals than the maternal milk feeding one, suggesting that the maternal milk might control the pepsinogen production in the proper gastric glands. The patterns of prochymosin expression in the goats was similar to that in cattle.

Anatomical study on the individual bones of the cranium of Korean native goat (한국재래산양 뇌두개의 분리골에 대한 해부학적 연구)

  • Yi, Seong-joon;Lee, Heungshik S
    • Korean Journal of Veterinary Research
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    • v.34 no.3
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    • pp.421-433
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    • 1994
  • This study was carried out to identify anatomical characteristics of the individual bones of the cranium of the Korean native goat. The pars lateralis and pans basilaris of the os occipitale were fused each other, whereas the squama occipitalis articulated with the pars lateralis, forming the sutrue. The sinus sphenoidale was formed in the corpus ossis presphenoidalis. The os prietale was observed as a single bone. In the os frontale, an fossa innominatum was found ventrolateral to the root of processus cornualis, and the orificium orbitale canalis supraorbitalis was also formed on the pars orbitalis. The meatus temporalis was formed in the medial side of the pars squama of the os temporalis. The lamina cribrosa of the os ethmoidale was membrane-like structure with many foramina. The labyrinth ethmoidalis was composed of four endoturbinalia and ten to twelve ectoturbinalia.

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Conservative neural symmetry of the caprine mandible

  • Pares-Casanova, Pere M.
    • Korean Journal of Veterinary Research
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    • v.53 no.4
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    • pp.207-210
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    • 2013
  • Fifteen dry dentate and complete mandible samples from the White Rasquera goat breed were studied for symmetry. Thirty-one landmarks were digitally located on the images of the lateral and medial aspects of each hemimandible. Distances between these landmarks allowed the evaluation of the whole hemimandible and also the neural mandible. In the studied samples, the mandible was rather symmetrical, especially in the medial neural part, and in general, there was no side dominance. Only the diastema differed significantly between the sides, and this was related to the rostral part (incisive arch). The incisive region was the least symmetrical region of the caprine mandible, indicating a modular structure more conservative for the neural part. If unsigned asymmetry is interpreted as a measure of developmental stability, then the studied breed presented a marked ability to develop in good fitness despite the harsh environment. The measurements presented here can also be used as a reference for researchers designing experimental studies, especially on mandibular catch-up growth, and as an aid for zooarchaeologists comparing results from dead animals with those from living goat populations.

Indirect Assement of Sperm Capacitation Using Zona-free Hamster Eggs in the Goat II. Penetration into Zona-free Hamster Eggs by Goat Spermatozoa Preincubated in a Chemically Defined Medium

  • Song, H.B.;Iritani, A.
    • Korean Journal of Animal Reproduction
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    • v.9 no.2
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    • pp.153-157
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    • 1985
  • Ejaculated and epidiymal goat spermatozoa were preserved for 0, 6, 12 adn 18 h, and 0 and 18 h in a semi-aerobic condition at 20-$25^{\circ}C$, and preincubated for 5-6 h in a CO2 incubator in m-KRB solution. Then they were preincubated at different concentrations (3-5, 25-48 and 105-190$\times$107/ml), and ability of penetration into zona-free hamster eggs in vitro was examined. When ejaculated spermatozoa were preincubated in m-KRB solution after presservation for 12 and 18 h, 12 and 29% of zona-free eggs were penetrated, and only 4% of eggs were penetrated by epididymal spermatozoa which were preincubated after preservation for 18 h. When spermatozoa were preincubated at a low concentration, the penetration rates were very low. But when the sperm concentration during preincubation was 25-48 and 105-190$\times$107/ml, the penetration rates increased to about 30%.

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