• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.10-14
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• 2000

In order to investigate the appropriate processing season and the production of high value-added products in ark shell(Scapharca subcrenata) cultured at the south coast of Korea, the foot muscle, mantle, and adductor muscle were analyzed for moisture, protein, fat, ash, and glycogen using specimens collected bimonthly from December 1994 to December 1995. The contents of moisture in foot muscle and mantle increased in spring season, however their proteins decreased in same season. Glycogen and fat in foot muscle, mantle, and adductor muscle were most abundant in June, just before the spawning season, but all the cmponsnts dwindled during spawning season. The other hand, the contents of ash in tissues were almost the same level through the year. Thus, the contents of moisture, protein, fat, and glycogen were almost the same level through the year. Thus, the contents of moisture, protein, aft, and glycogen were fluctuated by season. Especially, they showed a marked seasonal variation at before and after sparning season. So, a major cause of seasonal variation in S. subcrenata would be connected with a period of reproduction and spawning season.

• The Journal of the Korean dental association
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• v.11 no.5
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• pp.331-336
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• 1973

• Fisheries and Aquatic Sciences
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• v.4 no.1
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• pp.18-24
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• 2001

Seasonal content changes of the three key nutrients for organisms, protein, lipid and glycogen, were analyzed for a whole year to delineate the seasonal energy strategy in pen shells, Atrina pectinata. Two metabolically important organs, the visceral mass and the posterior adductor muscle, were examined. Protein in the visceral mass rose in April and stayed at the level until June followed by the constant minimum value between August and November. The protein contents in the posterior adductor muscle increased sharply in April and again in July, followed by a gradual decline thereafter. Total lipid contents in the visceral mass gradually increased between January and May, and then slowly decreased until September since which a new weak increase was noticed. Lipid levels in the adductor muscle rapidly dropped in June and July. Glycogen contents in the visceral mass rapidly increased between February and June, followed by a drastic drop in July. This reduced visceral glycogen level was maintained up to September, and a gradual reduction ensued. Glycogen contents in the adductor muscle steadily but markedly increased from April reaching the maximum in August, and then slowly declined thereafter. These results suggest that an accelerated protein and lipid synthesis occurs in the gonad when the pen shell undergoes the ripe stage of gametogenesis, but the levels of these two nutrients decrease on spawning. With this gonadal process, regular protein synthesis and lipid storage in the posterior adductor muscle are temporarily arrested. The most important nutrient reserves that support gonad developmental cycles in a long term seem to be glycogen of the posterior adductor muscle.

• Korean Journal of Exercise Nutrition
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• v.24 no.2
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• pp.1-5
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• 2020

[Purpose] Lactate has several beneficial roles as an energy resource and in metabolism. However, studies on the effects of oral administration of lactate on fat metabolism and glycogen synthesis are limited. Therefore, the purpose of the present study was to investigate how oral administration of lactate affects fat metabolism and glycogen synthesis factors at specific times (0, 30, 60, 120 min) after intake. [Methods] Male Sprague Dawley (SD) rats (n = 24) were divided into four groups as follows: the control group (0 min) was sacrificed immediately after oral lactate administration; the test groups were administered lactate (2 g/kg) and sacrificed after 30, 60, and 120 min. Skeletal muscle and liver mRNA expression of GLUT4, FAT/CD36, PDH, CS, PC and GYS2 was assessed using reverse transcription-polymerase chain reaction. [Results] GLUT4 and FAT/CD36 expression was significantly increased in skeletal muscle 120 min after lactate administration. PDH expression in skeletal muscle was altered at 30 and 120 min after lactate consumption, but was not significantly different compared to the control. CS, PC and GYS2 expression in liver was increased 60 min after lactate administration. [Conclusion] Our results indicate that exogenous lactate administration increases GLUT4 and FAT/CD36 expression in the muscle as well as glycogen synthase factors (PC, GYS2) in the liver after 60 min. Therefore, lactate supplementation may increase fat utilization as well as induce positive effects on glycogen synthesis in athletes.

• Nutritional Sciences
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• v.8 no.3
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• pp.181-188
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• 2005

We investigated the effects of diet manipulation on pro- and macro-glycogen accumulation and mobilization during exercise in different kinds of muscle fiber and tissue. Thirty-two Sprague-Dawley rats were divided into groups representing one of two dietary conditions: high fat (HF, n=16) or standard chow (CHOW, n=16). Each dietary group was fm1her divided into control (REST, n=8) and exercise (EXE, n=8). After an eight-week dietary intervention period, the animals in EXE swam for 3 hours while the animals in REST remained at rest Skeletal muscle (soleus, red gastrocnemius and white gastrocnemius) and liver samples were then dissected out and used for analyses. 1here was no statistical difference in body weight between the animals in the HF and mow groups (p>.05). Three hours of exercise significantly increased plasma free fatty acid (FFA) concentration in the animals in the CHOW group but not in the animals in the HF group. Both citrate. synthase (CS) and $\beta$-hydroxyacyl dehydrogenase ($\beta$-HAD) activities in skeletal muscles were higher in the HF group than in the mow group. CS and $\beta$-HAD activities were also the highest in red gastrocnemius and the lowest in white gastrocnemius. At both time points (i.e., rest and immediately after exercise) intramuscular triglyceride (IMTG) and liver TG concentrations were significantly higher in the HF compared to the CHOW. IMTG and liver TG changed selectively in the CHOW. Except in white gastrocnemius muscle, there was no significant difference in total glycogen content between HF and mow at rest. Although exercise significantly lowered total glycogen content in all groups and tissues (p<.05), the degree of reduction was markedly greater in the mow than in the HF. Whereas changes in proglycogen concentration showed a trend similar to those of total glycogen, alterations in macroglycogen concentrations clearly differed from those of total glycogen. Specifically, the degree of reduction of macroglycogen following three hours of exercise was substantially greater in the CHOW than in the HF. These results suggest that metabolic alterations induced by a long-term high fat diet may be caused by macro-glycogen rather than pro-glycogen.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.6 no.1
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• pp.15-23
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• 2006

Purpose: Glycogen storage disease type III (GSD-III), is a rare autosomal recessive disorder of glycogen metabolism. The affected enzyme is amylo-1,6-glucosidase, 4-alpha-glucanotransferase (AGL, glycogen debranching enzyme), which is responsible for the debranching of the glycogen molecule during catabolism. The disease has been demonstrated to show clinical and biochemical heterogeneity, reflecting the genotype-phenotype heterogeneity among different patients. In this study, we analyzed mutations of the AGL gene in three unrelated Korean GSD-III patients and discussed their clinical and laboratory implications. Methods: We studied three GSD-III patients and the clinical features were characterized. Sequence analysis of 35exons and part exon-intron boundaries of the AGLgene in patients were carried out by direct DNA sequencing method using genomic DNA isolated from patients' peripheral leukocytes. Results: The clinical features included hepatomegaly (in all patients), seizures (in patient 2), growth failure (in patients 1), hyperlipidemia (in patients 1 and 3), raised transaminases and creatinine kinase concentrations (in all patients) and mild EKG abnormalities (in patients 2). Liver transplantation was performed in patient 2due to progressive hepatic fibrosis. Administration of raw-corn-starch could maintain normoglycemia and improve the condition. DNA sequence analysis revealed mutations in 5 out of 6 alleles. Patient 1 was a compound heterozygote of c.1282 G>A (p.R428K) and c.1306delA (p.S603PfsX6), patient 2 with c.1510_1511insT (p.Y504LfsX10), and patient 3 with c.3416 T>C (p.L1139P) and c.l735+1 G>T (Y538_R578delfsX4) mutations. Except R428K mutation, 4 other mutations identified in3 patients were novel. Conclusion: GSD-III patients have variable phenotypic characteristics resembling GSD-Ia. The molecular defects in the AGL gene of Korean GSD-III patients were genetically heterogeneous.

• The Korean Journal of Zoology
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• v.11 no.2
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• pp.48-54
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• 1968

Male rats of the Albino strain received methylene blue in the dose of 40mg/kg by subcutaneous injection and were subjected to total body X-irradiation, 300 roentgen, at 30 minutes after the injection. The protective effect of methylene blue against the single total body X-irradiation was studied for 24 days after X-irradiation with regard to the levels of liver glycogen, blood glucose, and electrolytes in serum. 1. Total body X-irradiation generally casued an increase in the levels of the liver glycogen and blood glucose in both methylene blue treated and control group during the entire experiment. 2. Methylene blue has been shown to delay slightly the increase of the levels of the liver glycogen and blood glucose when comparing with both groups which were given methylene blue and control saline injection before irradiation in the rats. 3. The delay in the increase in the levels of liver glycogen, in experimental group injected with methylene blue, significantly came in two phases. The first phase appeared at there days after the exposure, the second followed at eighth day. It appeared that the recovery phase was at nineteenth day. 4. During the experimental days the levels of the blood glucose increased generally, methylene blue, however, caused delay in two phases; the first at fifth day, the second at eighteenth day after the exposure to X-rays. 5. In electrolytes, there was not a significant difference. The levels of chloride were, however, slightly decreased in both groups, levels of potassium appeared different in two phases at first day and twelfth day, and the levels of sodium appeared to show irregular changes at the same levels, but there was no significant difference. 6. It may be considered that methylene blue greatly reduces the sensitivity of rats to X-rays, provided that methylene blue is given before the exposure.

• Journal of Yeungnam Medical Science
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• v.15 no.1
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• pp.29-35
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• 1998

Rats were studied during 45 minutes treadmill exercise to determine the effects of hyperglycemia and hyperlipidemia on the utilization of cardiac muscle glycogen, and the utilization of diaphragm muscle glycogen was also studied for comparing to cardiac muscle. The hyperglycemia was produced by ingestion of 25% glucose solution(lml/100gm, BW) and the hyperlipidemia by 10% intralipose ingestion(lml/l00gm, BW) with intraperitoneal injection of heparin(500 IU) 15 minutes before treadmill exercise. The mean blood glucose concentrations(mg/dL) in control and hyperglycemic rats were 110 and 145, respectively, and the mean plasma free fatty acid concentrations(${\mu}Eq/L$) in control, control exercise(control-E) and hyperlipidemia exercise(HL-E) rats were 247, 260 and 444, respectively. In the hyperglycemic trial, the cardiac muscle glycogen concentration was not significantly decreased by the exercise but the concentration in control rats was decreased to 73.9%(p<0.05). The glycogen concentration of diaphragm was significantly decreased in both groups by the exercise, but the hyperglycemia decreased the glycogen utilization by approximately 10% compared to the control. The cardiac muscle glycogen concentration was not decreased by the exercise in control and hyperlipidemic rats but the utilization of glycogen in hyperlipidemic rats is lower than that of the control. These data illustrate the sparing effect of hyperglycemia on cardiac muscle glycogen usage during exercise, but the effect of hyperlipidemia was not conclusive. In the skeletal muscle, the usage of glycogen by exercise was spared by both hyperglycemia and hyperlipidemia.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1700-1707
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• 2011

This study evaluated the effect of multigrain supplementation on exercise-related changes in running time and glycogen storage capacity of male SD rats engaged in treadmill exercise. Thirty-six 6-week-old rats were divided into six groups: normal (Nor) fed normal feed, control (Con) fed with 20% normal feed and 80% milled rice, test group one (GI) fed with multigrain feed I, test group two (GII) fed with multigrain feed II, test group three (GIII) fed with multigrain feed III, and test group four (GIV) fed with multigrain feed IV. Endurance tests by treadmill machine were administered after 24 days of multigrain feed supplementation and adaptive training. Running time was extended and glycogen storage capacity increased in the multigrain-treated group compared to the non-treated group. Also, the fatigue indicators of inorganic phosphorous, CPK, and lactate concentration were all reduced in the multigrain feed group compared to the control group after 25 min and/or exhausted exercise. But there was no difference in GOP, GTP, lactate, or LDH concentrations between the groups. Our results demonstrated that endurance improved with multigrain feed in rats. Specifically, running time, glycogen storage capacity, inorganic phosphorous, CPK, and lactate serum concentration increased. Importantly, the improvements in endurance brought about in the GII group fed with waxy barley was the greatest among the experimental groups.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.7-11
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• 2001

The effect of topical application with 200,400 and 600 $\mu$g/ml 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Naphthoxyacetic Acid (NOA) to the fifth stadium larvae of the silkworm, B. mori on fat body glycogen, protein, total lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen significantly increased in all the groups treated with 2,4-D whereas there is no significantly change in fat body glycogen in all the groups treated with NDA. However, the haemolymph trehalose was significantly increased in all 274-D and NOA treated groups except in the groups treated with 400 and 600 $\mu$g/ml 2,4-D where the increase was not significant.The fat body protein did not show any significant change in all groups treated with 2,4-D and NOA except in the groups treated with 200 $\mu$g/ml 2,4-D where the fat body protein was significantly increased. The total lipids, phospholipids and neutral lipids of the fat body increased significantly in all the groups treated with 2,4-D and NOA when compared with those of the corresponding parameters of the carrier control.