• Applied Biological Chemistry
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• 제20권2호
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• pp.205-209
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• 1977

단일주사방법(單一注射方法)에 의(依)하여 정상산란계(正常産卵鷄)의 포도당대사(代謝)에 관련(關聯)되는 혈액(血液)포도당의 대사(代謝)풀(pool)크기, 대사회전속도(代謝回轉速度), 대사회전시간(代謝回轉時間), 기타(其他) 관계(關係)되는 항목(項目)들을 조사(調査)하였다. 날개의 정맥(靜脈)을 통(通)하여 정상적(正常的)으로 사양(飼養)된 산란계(産卵鷄)에 $11.6{\mu}Ci$의 순수(純粹)포도당-$C^{14}$를 주입(注入)하였으며 주사(注射) 5분후(分後)에 채취(採取)한 혈액(血液)의 원심분리(遠心分離)에 의(衣)하여 얻어진 혈장(血漿)의 포도당 농도(濃度)는 100ml당(當) 214mg이였다. 혈장(血漿)포도당의 표준비방사능(標準比放射能)의 대수(對數)를, 주사후(注射後) 혈액(血液)을 채취(採取)한 시간(時間)(5분(分)부터 120분(分)까지)에 대(對)하여 그런 곡선(曲線)과 그의 회귀방정식(回歸方程式)에 대(依)하여 여러가지 대사상태(代謝狀態)를 측정(測定)하였다. 포도당 대사(代謝)의 풀(pool)크기는 1.07g이었고 대사회전속도(代謝回轉速度)는 1분간(分間) 0.024이었으며 대사회전시간(代謝回轉時間)은 41분(分)이었다. 포도당의 이용속도(利用速度)는 1분간(分間) 26mg이였으며(0.75승(乘)한, 즉(卽) 대사체중(代謝體重) kg당(當)) 1시간당(時間當) 0.83g이었다. 포도당 공간(空間)(세포외(細胞外) 체액(體液)의 용량(容量))은 체중(體重)의 25.3이었다. 주사후(注謝後) 10분(分)부터 50분(分)까지 채취(採取)한 시료(試料)로부터 얻은 결과(結果)도 상기(上記)의 5분(分)부터 120분(分)까지의 시료(試料)에서 얻은 성적(成績)과 비등(比等)하였다. 시일경과(時日經過)함에 따라 75%까지 억제하였다. 6)산 초 산초 1%, 5% 첨가구(添加區)는 저장시일(貯臧時日)과 관계(關係)없이 50% 억제하였고 10% 첨가구(添加區)는 3일이후(日以後)부터 계속 억제하여 75%까지 억제 하였다. 7)겨 자 겨자 1%, 5% 첨가구(添加區) 대조구(對照區)에 비(比)하여 ${\alpha}-amylase$ 활성(活性)을 50% 억제 하였으나 10% 첨가구(添加區)는 시일경과(時日經過)에 따라 62.5%의 억제 현상을 보였다. 8) 고초냉이 고초냉이 1%, 5% 첨가구(添加區)는 ${\alpha}-amylase$ 활성(活性)을 50% 억제 하였으나 10% 첨가구(添加區)는 87.5%까지 억제하였다. 9) Mono sodium glutamate Mono sodium glutamate 1% 첨가구(添加區)는 5일(日)까지는 ${\alpha}-amylase$ 활성(活性)을 억제하지 않았으나 그후(後)부터 50% 억제하였고 5%, 10%, 첨가구(添加區)는 시일(時日)이 경과(經過)함에따라 50%에서 82.5%로 억제되었다. 10)설 탕 설탕 1%, 5% 첨가구(添加區)는 저장(貯臧) 3일(日)까지는 50%로, 그 이후(以後)는 75% 억제되었고 설탕 10% 첨가구(添加區)는 계속 75% 억제 하였다.ato granules and their cooked ones. 8. From the view of food sanitation, dipping treatment of the potato granules in the solution of $K_2S_20_5$ is safe because of the few residual amount of

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1518-1518
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• 2001

In the area of the destruction-free NIR analysis of fruit and vegetables development has not yet progressed as far as in grain and similar products. One reason for that is, that in contrast to grains, in fruit and vegetables water appears as the outstanding main-component making up typically 80% by weight of the fruit. Of the M absorption spectrum of pure water the bands at 1450, 970 and 760 nm are the first, second and third overtones respectively of O-H stretch while those at 1940 and 1190 are combination bands involving O-H stretch and O-H bend. The choice of band for spectrometry is governed by considerations of sensitivity and selectivity. The overtone bands are satisfactory for use in moisture measurements from 0 to 4 % depending on path length. Measurements in fruits and vegetables at wavelength areas that are also important for the determination of carbohydrates (sucrose, glucose, fructose) often lead to total absorption in the presence of significant water even if short path lengths are possible. In this work model systems are used containing different carbohydrates in solvents like heavy water (D$_2$O) or dimethylsulfoxide (DMSO) that do not contain O-H functional groups.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.524-530
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• 2011

In the present study, we demonstrate that SRM LC-MS/MS method developed by Luo et al. (ref. 10) can be successfully applied to the quantitative analysis of intracellular metabolites in E. coli that are collected at the exponential and stationary growth phases. A focus is given on measuring the changes in the concentrations of intracellular metabolites in batch cultures, which were induced during both the dynamically changing exponential and stationary growth phases. The following intracellular metabolites are quantified in the exponential and stationary phases of E. coli growth, using the SRM mode of a triple quadrupole mass spectrometer: glucose-1-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, pyruvate, acetyl-coenzyme A, 6-phosphogluconate, ribulose-5-phosphate, xylulose-5-phosphate, erythrose-4-phosphate. The determined intracellular metabolite concentration profiles are shown to be in a good agreement with the growth profiles of E. coli, which clearly indicates that SRM LC-MS/MS can be successfully used for following the metabolite changes induced at different growth stages.

• Natural Product Sciences
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• 제3권1호
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• pp.38-41
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• 1997

The hypoglycemic effect of the extract of Ficus racemosa leaves was studied on streptozotocin-induced diabetic rats. Petroleum ether $(60-80^{\circ}C)$ extract of the plant obtained by soxhlet extraction from coarsely pulverised leaves was used. In the $LD_{50}$ determination of the extract no abnormalities were observed at the dose range of 3 g/kg (p.o.) of the extract. The extract (200 mg/kg and 400 mg/kg orally) caused a reduction of blood glucose levels in streptozotocin-induced diabetic rats by 28.9% (P<0.00l) and 34.6% (P<0.001) respectively at the end of 9 days. The results. of this study indicate that the petroleum ether $(60-80^{\circ}C)$ extract of the leaves possesses significant hypoglycemic activity in hyperglycemic animals compared with glybenclamide as standard drug.

• 한국미생물·생명공학회지
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• 제32권4호
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• pp.366-370
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• 2004

Four chitinase producing bacteria, Arthrobacter nicotinae CH4, Arthrobacter nicotinae CHI3, Arthrobacter sp. CH5 and Micrococcus sp. CH3, were isolated from small crabs and shrimps. We investigated the optimum medium condition for the production of enzyme and high cell mass. The preferable medium composition was as follows: colchitin 0.1 %(w/v), glycerol 0.25%(w/v) and yeast extract 0.05%(w/v) in minimal midium ($K_{2}HPO_{4}$ 0.7 g/l, $KH_{2}PO_{4}$ 0.3 g/l, $MgSO_{4}{\cdot}5H_{2}O$ 0.5 g/l, $FeSO_{4}}{\cdot}7H_{2}O$ 0.01 g/l, $ZnSO_{4}$ 0.001 g/l, $MnCI_2$ 0.001 g/l, pH 7.0). This cell culture medium could be used directly as sample for measuring chitinase activity. Because it hardly conreducing sugar such as glucose (blank value=0), the detected reducing sugar can be considered as a chitinase reaction product. The results can be used for easy preparation method for determination of enzyme activity and analysis of enzyme-substrate reaction in step of screening of chitinase producing bacteria.

• 펄프종이기술
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• 제40권4호
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• pp.43-50
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• 2008

Adsorption of xylans extracted from birchwood and oat spelt on Hw-BKP were analyzed using HPLC. The effect of xylan adsorption on paper properties such as tensile, tear index and brightness was also investigated. The constituents of xylan was analyzed with HPLC after hydrolysis with dilute sulfuric acid. It was shown that xylose was the major constituent and small amounts of glucose and galactose were contained in the xylan samples. Adsorption of xylan on hardwood fibers was evaluated using acid hydrolysis and HPLC techniques. Results showed that the adsorption of negatively charged xylan on the fiber surface was negligible probably because electrostatic repulsion between these two materials. Pretreatment of the fiber with alum increased xylan adsorption. The amount of adsorption increased up to 30 mg/g. With the increase of xylan adsorption both tensile and tear strength of the handsheet increased suggesting xylan can be a very effective strength agents for papermaking. Brightness of the handsheets decreased, however, with the use of xylan.

• 한국식품조리과학회지
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• 제8권3호
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• pp.247-253
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• 1992

The total dietary fiber (TDF) contents in cultivated dodok, wild dodok and ginseng were determined by AOAC method and Mongeau et al's method. Also, the nonstarch polysaccharide (NSP) contents of them were analyzed by Englyst et al's gas chromatographic (GC) method. The TDF values by AOAC method and Mongeau et al's method were 34.50% and 35.92% for wild dodok, 46.40% and 47.55% for cultivated dodok, and 14.93% and 14.03% for ginseng, respectively. The ratios of soluble dietary fiber (SDF) contents to TDF contents by Mongesu et al's method were 64.17% for wild dodok, 75.77% for cultivated dodok, and 53.74% for ginseng, respectively. The major sugar constituents of NSF in wild dodok, cultivated dodok and ginseng by GC were glucose, galactose and uronic acid. The Enalyst's TDF contents, i.e., NSP plus lignin contents in wild dodok, cultivated dodok, and ginseng were 20.65%, 20.03% , and 9.72%, respectively.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.560-567
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• 2005

Three groups of exopolysaccharides (EPSs) (designated as Fr-I, Fr-II, and Fr-III) were isolated from the culture filtrates of Paecilomyces sinclairii by gel filtration chromatography on Sepharose CL-4B. Their molecular characteristics were examined by multi-angle laser light scattering (MALLS) connected online to a size exclusion chromatography (SEC) and refractive index (RI) detector system. The weight-average molar mass of Fr-I, Fr-II, and Fr-III of EPSs were determined to be $1.540{\times}10^6,\;6.302{\times}10^4$, and $9.389{\times}10^4\;g/mol$, respectively. All three EPSs showed a fairly low polydispersity indice, ranging from 1.008 to 1.059 (nearly mono disperse behavior), and showed different carbohydrates and amino acids compositions; all fractions of EPSs consisted of mainly cystine, valine, and arginine in the protein moiety, and mainly ribose, galactose, and glucose in the carbohydrate moiety. The determination of gyration radii of the EPSs in SEC/MALLS analysis revealed the molecular shape of the Fr-I to be a rod-like structure, whereas the Fr-II and Fr-III had a random-coil structure in an aqueous solution.

• Bulletin of the Korean Chemical Society
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• 제26권10호
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• pp.1533-1538
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• 2005

We have determined the contents of monosaccharides in the hydrolysates of some yogurt and orange juice products by derivatizing monosaccharides with p-aminobenzoic acid ethyl ester (ABEE). The separation of the ABEE-derivatized monosaccharides was efficiently carried out by HPLC using a microcolumn packed with the Alltima $C_{18}$ stationary phase. The concentrations of monosaccharides were determined based on the measured peak area/height counts. ABEE derivatization of fructose and its detection have never been successfully carried out before this work. In this study, two peaks were observed in a fixed ratio for ABEE-fructose, and the ratio was maintained over a wide range of fructose concentration. In order to prove the validity of the above method, we compared the concentrations of glucose, galactose and fructose determined by ABEE derivatization and UVD (ultraviolet detector) chromatography with those determined by RID (refractive index detector) chromatography without derivatization. The determined concentrations of monosaccharides obtained from the two chromatographic methods were found close to each other within acceptable error ranges.

• Food Science and Biotechnology
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• 제14권3호
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• pp.344-349
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• 2005

Aldose reductase catalyzes the conversion of glucose into sorbitol. Inhibiting this enzyme in diabetes mellitus can delay or prevent pathogenic process. Aldose reductase inhibitor was screened from Korean fermented soybean pastes (Doen-jang) and purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. Aldose reductase inhibitor was identified as genistein with molecular weight of 270 Da and molecular formula of $C_{15}H_{10}O_5$ based on UV spectrometry, $^1H$ and $^{13}C\;NMRs$, and mass spectrometry. Genistein inhibited aldose reductase of pig lens with $IC_{50}$ level of $20\;{\mu}M$. Because genistein was effective against aldose reductase of animal source, it may be a potential therapeutic agent for diabetic complications.