Bulletin of the Korean Chemical Society

  • 제32권2호
  • /
  • Pages.524-530
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  • 2011
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  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Determination of the Intracellular Concentrations of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry

  • Park, Chang-Hun (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Park, Chang-Hun (Department of Chemistry, Sogang University) ;
  • Lee, Youn-Jin (Department of Chemistry, Sogang University) ;
  • Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Oh, Han-Bin (Department of Chemistry, Sogang University) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • 투고 : 2010.09.30
  • 심사 : 2010.12.03
  • 발행 : 2011.02.20
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초록

In the present study, we demonstrate that SRM LC-MS/MS method developed by Luo et al. (ref. 10) can be successfully applied to the quantitative analysis of intracellular metabolites in E. coli that are collected at the exponential and stationary growth phases. A focus is given on measuring the changes in the concentrations of intracellular metabolites in batch cultures, which were induced during both the dynamically changing exponential and stationary growth phases. The following intracellular metabolites are quantified in the exponential and stationary phases of E. coli growth, using the SRM mode of a triple quadrupole mass spectrometer: glucose-1-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, pyruvate, acetyl-coenzyme A, 6-phosphogluconate, ribulose-5-phosphate, xylulose-5-phosphate, erythrose-4-phosphate. The determined intracellular metabolite concentration profiles are shown to be in a good agreement with the growth profiles of E. coli, which clearly indicates that SRM LC-MS/MS can be successfully used for following the metabolite changes induced at different growth stages.

키워드

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