• Title/Summary/Keyword: glucan

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Changes in Physicochemical Characteristics of Immature Barley Kernels during Roasting (미숙보리곡립의 볶음중 이화학적 특성변화)

  • Lee, Young-Tack;Seog, Ho-Moon;Kim, Sung-Soo;Kim, Kyung-Tack;Hong, Hee-Do
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.336-342
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    • 1994
  • Changes in physicochemical characteristics were investigated for immature barley kernels roasted at $160{\sim}220^{\circ}C$ for $1{\sim}12$ min. Only small differences in chemical constituents including starch, protein, fat, ash, total dietary fiber, and ${\beta}-glucan were observed between immature and mature barley kernels. The amounts of 75% ethanol-soluble sugars and amino acids present in immature barley kernels were considerably higher than those in mature kernels, and gradually decreased in the process of roasting. Of free sugars, sucrose, raffinose, glucodifructose($GF_{2}$) and maltose were reduced by roasting. Glucose and fructose, simple reducing sugars, decreased at the early stage of roasting, followed by a slight increase at the later stage. Starch and nitrogen contents decreased slowly, while TDF(total dietary fiber) had a tendency to increase slightly. Stacking volume of immature barley kernels increased markedly, especially at the higher temperatures. L value of immature barley decreased throughout roasting, and a, b values increased at the early stage of roasting but b value decreased with continued roasting. The degree of roasting was strongly affected by the roasting temperature. Darkness of immature barley kernel, depending on the degree of roasting, was highly associated with concentrations of brown pigments extracted from roasted immature barley kernels.

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Synergistic Inhibition of IgY, Auricularia auricula, and Lactic Acid Bacteria from Kimchi and Tarak on Helicobacter pylori (Helicobacter pylori 의 생육억제에 대한 유산균, 난황항체 및 목이버섯의 상승효과)

  • Yoo, Hye-Lim;Lee, Young-Duck;Han, Bok-Kyung;Choi, Hyuk-Joon;Park, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.26 no.1
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    • pp.35-43
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    • 2013
  • The substances of lactic acid bacteria (LAB) isolated feom Kimchi and Tarak, L. mesenteriodes LAB kw5, and S. thermophilus LAB KW15 were investigated for growth effect of Helicobacter pylori with IgY and Auricularia auricula. Inhibition of H. pylori was confirmed at LAB KW5 and KW15 supernatants. Interestingly, anti-H. pylori substance in LAB KW5 and KW15 supernatants were sensitive to lipase, but insensitive to protein hydrolase and carbohydrate hydrolase. The inhibition zone toward H. pylori was not shown with the lipase-treated supernatants. Therefore, there seemed to be lipid-like substances in the cultures. By the analyses with gas chromatography, undecanoic acid ($C_{11:0}$), palmitic acid ($C_{16:0}$), stearic acid ($C_{18:0}$), and oleic acid ($C_{18:1}$) were detected at the culture substances from L. mesenteroides LAB KW5 and S. thermophilus LAB KW15, and more eicosadienoic acid ($C_{20:2}$) from L. mesenteroides LAB KW5. Anti-H. pylori substances of LAB with IgY and A. auricula extract were analyzed for inhibition effect of H. pylori. The inhibition increased more by the range from 57% to 86% by the mixture. The substances with IgY and A. auricula extract showed more effective inhibition of H. pylori than single or double trials.

Bioethanol Production from Wasted Corn Stalk from Gangwon Province : from Enzymatic Hydrolysis to Fermentation (강원지역 폐옥수수대로부터 바이오에탄올 생산 : 효소 당화부터 발효까지)

  • Choi, Jae Min;Choi, Suk Soon;Yeom, Sung Ho
    • Applied Chemistry for Engineering
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    • v.23 no.3
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    • pp.326-332
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    • 2012
  • Among the samples prepared by various pre-treatment methods, the one pretreated by dilute sulfuric acid showed the highest glucose yield in the enzymatic hydrolysis. Statistical analysis of enzymatic hydrolysis revealed that the glucose yield was in proportion to the enzyme dosage, the ratio of the pre-treated sample to the buffer solution, and the reaction time and that the effect of enzyme dosage was predominant in the experiment range. In addition, the glucose yield was estimated to be 76.1% at an optimal enzymatic hydrolysis condition. In a separate hydrolysis and fermentation (SHF), Saccharomyces cerevisiae converted over 80% of glucose from the enzymatic hydrolysis of pre-treated wasted corn stalk by dilute sulfuric acid to bioethanol with 37% of ethanol yield and 0.42 $g/L{\cdot}hr$ of productivity. In the simultaneous saccharification and fermentation (SSF), 59.5% of conversion from glucan to ethanol and 0.20 $g/L{\cdot}hr$ of productivity were achieved. In both SHF and SSF, approximately 88 g of bioethanol could be obtained from 1 kg of wasted corn stalk. The possible amount of bioethanol in Gangwon province were estimated to be 1.9 kiloton with the assumption of the 50% of collection ratio.

The Analysis of Effect of Biopolymer Treated Soils in Seed Spray Method in the River Embankment (제방 녹화공법에서 바이오폴리머 처리토의 효능 분석)

  • Seo, Seunghwan;Jin, Seungnam;Chang, Ilhan;Chung, Moonkyung
    • Ecology and Resilient Infrastructure
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    • v.6 no.4
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    • pp.304-313
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    • 2019
  • Biopolymer based on microbial β-glucan and xanthan gum is effective for vegetation and has a function of enhancing soil strength, which can be used as soil reinforcement and stabilization materials in river embankment. The purpose of this study is to verify the vegetation effect of the surface of levee by biopolymer with seed spraying method. Mixed soils with biopolymer were used to cover the surface of embankments. The strength is higher in biopolymer-treated soil and xanthan gum based biopolymer has advantage for quality control in field scale. In addition, the vegetation of F. arundinacea and L. perenne showed various reactions with types of biopolymers. Biopolymer has a positive effect on the vegetation of them. In contrast, root growth tended to decrease in biopolymer-treated soils. The results indicate that root growth is slow down due to increasing ability to retain water in biopolymer-treated soil. In order to apply biopolymer to river embankment, it is necessary to examine the effects of biopolymers on a wide range of plant species in river embankment.

Isolation of an Anticariogenic Compound from Magnoliae Bark (후박피(Magnoliae bark)로부터 항충치활성을 갖는 물질의 분리)

  • Lee, Youn-Soo;Park, Hun-Joo;You, Jae-Sun;Park, Hyung-Hwan;Kwon, Ik-Boo;Lee, Hyeon-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.1
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    • pp.230-236
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    • 1998
  • We have screened total 32 herbal drugs to find the highest activity against human cariogenic enzyme, glucosyltransferase (GTase) from the extracts of Magnoliae bark. The extracts were separated into three phases, i. e. water, n-butanol and ethylacetate according to their solvent polarity. Among them, ethylacetate fraction had approximately more than 70% of total activities, and the active principle was further isolated by prep. HPLC following silicagel column chromatography to yield single compound as white powder. The chemical structure of the compound was finally elucidated to be 4,4'-dihydroxy-3,3'-dimethoxylignan from the spectral data of FAB-MS. $^1H-\;and\;^{13}C-NMR$ spectrometries. The compound was also shown to have relatively strong antibacterial activity against ten types of cariogenic oral bacteria and one kind of Actinomyces sp.

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Biological Activities of Polysaccharide Extracted from the Fruit Body and Cultured Mycelia of Phellinus linteus IY001 (목질진흙버섯 자실체와 배양 균사체 유래 ${\beta}-Glucan$성 다당류의 생리활성)

  • Lee, June-Woo;Baek, Sung-Jin;Bang, Kwang-Woong;Kang, Shin-Wook;Kang, Sang-Mo;Kim, Byung-Yong;Ha, Ick-Su
    • Korean Journal of Food Science and Technology
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    • v.32 no.3
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    • pp.726-735
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    • 2000
  • This study was conducted to investigate the biological activities of polysaccharide extracted from the fruit body and cultured mycelia of Phellinus linteus IY001. All fractions were extracted by hot water, in the next, Fr. I, Fr. II, Fr. III and Fr. IV were polysaccharide obtained by ethanol precipitation or ultrafiltration. The highest antitumor activity against sarcoma 180 in ICR mice was observed in Fr. III and Fr. IV at the level 85%, but the antitumor activity had no connection with their anticomplementary activity in vitro, it might probably be due to extraction of hot water. All fractions promoted the production of nitric oxide and $TNF-{\alpha}$ in macrophage, addition of Fr. I and Fr. II resulted in production of nitric oxide$3(5.9{\sim}37.6\;{\mu}M)$ and of the $TNF-{\alpha}$ production($8,696.2{\sim}9,420pg/ml)$. All fractions inhibited the lipid peroxidation induced by $AsA/Fe^{2+}$, $ADP/NADPH/Fe^{3+}\;and\;CCl_4/NADPH$ in rat liver microsomes, and Fr. III showed the electron donating ability stronger than tocopherol in assay system using DPPH. From these results, it is suggested that all fractions contain immunoregulatory components which may protect cellular materials from the oxidative damages by their radical scavenging activities.

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Comparative energy content and amino acid digestibility of barley obtained from diverse sources fed to growing pigs

  • Wang, Hong Liang;Shi, Meng;Xu, Xiao;Ma, Xiao Kang;Liu, Ling;Piao, Xiang Shu
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.7
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    • pp.999-1005
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    • 2017
  • Objective: Two experiments were conducted to determine the content of digestible energy (DE) and metabolizable energy (ME) as well as the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in barley grains obtained from Australia, France or Canada. Methods: In Exp. 1, 18 growing barrows ($Duroc{\times}Landrace{\times}Yorkshire$; $31.5{\pm}3.2kg$) were individually placed in stainless-steel metabolism crates ($1.4{\times}0.7{\times}0.6m$) and randomly allotted to 1 of 3 test diets. In Exp. 2, eight crossbred pigs ($30.9{\pm}1.8kg$) were allotted to a replicate $3{\times}4$ Youden Square designed experiment with three periods and four diets. Two pigs received each diet during each test period. The diets included one nitrogen-free diet and three test diets. Results: The relative amounts of gross energy (GE), CP, and all AA in the Canadian barley were higher than those in Australian and French barley while higher concentrations of neutral detergent fiber, acid detergent fiber, total dietary fiber, insoluble dietary fiber and ${\beta}-glucan$ as well as lower concentrations of GE and ether extract were observed in the French barley compared with the other two barley sources. The DE and ME as well as the SID of histidine, isoleucine, leucine and phenylalanine in Canadian barley were higher (p<0.05) than those in French barley but did not differ from Australian barley. Conclusion: Differences in the chemical composition, energy content and the SID and AID of AA were observed among barley sources obtained from three countries. The feeding value of barley from Canada and Australia was superior to barley obtained from France which is important information in developing feeding systems for growing pigs where imported grains are used.

Cell migration and Anti-inflammatory Effect of Red Ginseng Extracts Fermented with Laetiporus Sulphureus (붉은덕다리버섯 균사체로 발효한 홍삼 배양액의 cell migration 및 항염 효능에 관한 연구)

  • Oh, Seong-Hwa;Choi, Soo-Yeon;Lee, Nu Rim;Lee, Jung No;Kim, Dong-Seok;Lee, Sang-Hwa;Park, Sung-Min
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.3
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    • pp.297-305
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    • 2014
  • Red ginseng (RG) contains specific ginsenosides (Rg2, Rg3) which show various pharmacological effects and absorption rate in the body better than panax ginseng. Therefore many people have been used it for health for a long time. Furthermore, many researchers have been studying its biological activities for a long times because fermentation generates lots of beneficial small molecules good for health. In this study, we fermented red ginseng with mycelium of Leatiporus sulphures var. miniatus for 7 days. As a result, we found that three ginsenosides Rg1, Re and Rb2 were decreased from 0.24, 0.25, 0.16 mg/g to 0.12, 0.1, 0.03 mg/g respectively HPLC analysis. In addition, we studied biological activities of fermented red ginseng (FRG) about skin ageing such as anti-inflammation, cell migration, anti-oxidation, collagen type 1 synthesis, and MMP-1 inhibition activities. As a result, FRG were shown higher anti-inflammatory and cell migration promoting activities than RG. FRG inhibited production of nitric oxide (NO) and mRNA expression of inducible nitric oxide synthase (iNOS) and decreased interleukin (IL)-6 induced by LPS stimulation in RAW 264.7 cells. In conclusion, this study suggest that FRG could be a potential source as a new natural anti-inflammatory agent.

Quality Characteristics of Muffins using Ethanol Fermentation By-product of Triticale (라이밀의 에탄올 생산 후 발효 부산물을 이용한 머핀의 품질 특성)

  • Seo, Eun-Ok;Ko, Seong-Hye;Lee, Nae-Taek;Kim, Kwang-Oh;Choi, Gi-Wook;Oh, Kyung-Chul
    • Journal of the East Asian Society of Dietary Life
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    • v.20 no.3
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    • pp.382-388
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    • 2010
  • This study was aimed to examine the quality characteristics of the bread with the muffin added with the by-product of fermented ethanol from wheat rye that has high $\beta$-glucan contents. In the muffin added with TEFB (Triticale Ethanol Fermentation By-product), the height of the muffin decreased as the additive contents increased. There was no significant difference among the volumes of the muffins added with TEFB 0%, 5%, 10% and 15%. In the muffin added with TEFB, the height of the muffin decreased as the additive contents increased. In the moisture measurement of TEFB muffin, the moisture contents increased as the TEFB additive contents increased. Using SEM, showed that as TEFB additive contents increased, gluten contents was relatively lowered, which led to the reduced gas retention power and generation of rough tissues. In chromaticity, as the TEFB additive contents increased, the brightness decreased and the redness and yellowness decreased as well. The result of texture measurement showed that as the TEFB additive contents increased, hardness increased. springiness decreased gradually as the TEFB additive contents increased. There was no significant difference in cohesiveness (p<0.005). Although there as signigicant difference between the control group and the additive group in gumminess (p<0.005), there was no significant difference for a certain amount of additive contents (p<0.05). While there was significant difference in chewiness between the control group and the additive group (p<0.05). there was no significant difference for a certain amount of additive contents (p<0.05). The result of taste test of TEFB added muffin showed that the preference for the muffins added with 5% or more TEFB decreased. This study found that more than 5% of TEFB additives to muffin decreases the preference level.

The optimization of extraction process on hemicellulose from rice bran (미강유래 식이섬유 헤미셀룰로오스의 추출 공정 최적화)

  • Jung, Ji-Eun;Choi, Yong-Hee
    • Food Science and Preservation
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    • v.15 no.4
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    • pp.532-541
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    • 2008
  • A central composite design was applied to investigate the effects of the independent variables of NaOH concentration(X1) and extraction time(X2) on dependent variables such as Yield(Y1), Xyl/Ara ratio(Y2), uronic acid(Y3), $\beta$-glucan(Y4) and total sugars(Y5) of hemicelluloses separated from rice bran. The Coefficients of determination(R2) in various models ranged from 0.8626 to 0.9319. Yield increased with increased NaOH concentration and extraction time. The optimum extraction conditions were NaOH concentration at 2.45M and extraction time of 24.2 h. Predicted values at the optimized conditions were acceptable, compared with experimental values. The structural characteristics of an optimum hemicellulose extract were explored. As a result, it showed that the surfaces of hemicellulose had a highly irregular reticulated structure. And also it was both small and large molecular particle in the hemicelluloses. Their average molecular weights were in the ranges $235{\sim}240$ kDa and $8.0{\sim}9.4kDa$, respectively.