• Korean Journal of Microbiology
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• v.42 no.4
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• pp.239-245
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• 2006

Leucine-responsive Regulatory Protein (Lrp) is a global regulator involved in modulating a variety of metabolic functions, including the catabolism and anabolism of amino acids as well as pili synthesis. In addition, there is growing evidences that Lrp may play an important role when cells make transition between rich and lean nutritional conditions. In this review, the biochemical characteristics of Lrp are described to provide a good example that shows how bacteria adapt to nutrient limitation and environmental stress.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.325-334
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• 2007

Recently, quorum sensing has been implicated as an important global regulator controlling the production of numerous virulence factors such as capsular polysaccharides in bacterial pathogens. The nucleotide and deduced amino acid sequences of smcR, a homolog of V. harveyi luxR identified from V. vulnificus ATCC29307, were analyzed. The amino acid sequence of SmcR from V. vulnificus was 72 to 92% similar to those of LuxR homologs from Vibrio spp. Functions of SmcR were assessed by the construction of an isogenic mutant, whose smcR gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of smcR resulted in a significant alteration in biofilm formation, in type of colony morphology, and in motility. When compared with the wild-type, the smcR mutant exhibited reduced survival under adverse conditions, such as acidic pH and hyperosmotic stress. The smcR mutant exhibited decreased cytotoxic activity toward INT 407 cells in vitro. Furthermore, the intraperitoneal $LD_{50}$ of the smcR mutant was approximately $10^2$ times higher than that of parental wild-type. Therefore, it appears that SmcR is a novel global regulator, controlling numerous genes contributing to the pathogenesis as well as survival of V. vulnificus.

• Journal of the Korean Institute of Intelligent Systems
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• v.12 no.6
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• pp.554-558
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• 2002

In this paper, we propose a robust indirect adaptive fuzzy state feedback regulator based on Takagi-Sugeno fuzzy model. The proposed adaptive fuzzy regulator is less sensitive to singularity than the conventional one based on the feedback linearization method. Furthermore, the proposed control method is applicable to not only plants with a perfect model but also plants with an imperfect model, which causes uncertainties. We verify the global stability of the proposed method by using Lyapunov method. In order to support the achievement, the application of the proposed adaptive fuzzy regulator to the control of a nonlinear system under the external disturbance is presented and the performance was verified by some simulation result.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.100.2-101
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• 2003

The global regulator, GacS (global antibiotic and cyanide sensor kinase), was required for the increased resistance to hydrogen peroxide occurring as cultures of the rhizobacterium, P. chlororaphis O6, matured. Specific stationary-phase peroxidase and catalase isozymes were absent in the GacS mutant, whereas a manganese-superoxide dismutase isozyme was expressed earlier and to a great extent than wild type. In the wild type cell, transcript accumulation of rpoS was higher in late logarithmic-phase cells than cells from mid logarithmic- or stationary-phase. Transcripts from rpoS in the GacS mutant were reduced in each of these growth phases compared to the wild type expression. The down stream sequence from rpoS lacked sequences encoding a small RNA, rsmZ, found in other pseudomonads and implicated in control of genes activated by the GacS system. These findings suggest that GacS-mediated regulation of RpoS plays role in control of oxidative stress in P. chlororaphis O6 by as yet an unknown mechanism.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2002.05a
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• pp.211-214
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• 2002

In this paper, we propose a robust indirect adaptive fuzzy state feedback regulator based on Takagi-Sugeno fuzzy model. The proposed adaptive fuzzy regulator is less sensitive to singularity than the conventional one based on the feedback linearization method. Furthermore, the proposed control method is applicable to not only plants with a perfect model but also plants with an imperfect model, which causes uncertainties. We verify the global stability of the proposed method by using Lyapunov method. In order to support the achievement, the application of the proposed adaptive fuzzy regulator to the control of a nonlinear system under the external disturbance is presented and the performance was verified by some simulation result.

• Proceedings of the IEEK Conference
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• 2002.07c
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• pp.1471-1474
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• 2002

In this paper, we propose a robust indirect adaptive fuzzy state feedback regulator based on Takagi-Sugeno fuzzy model. The proposed adaptive fuzzy regulator is less sensitive to singularity than the conventional one based on the feedback linearization method. Furthermore, the proposed control method is applicable to not only plants with a perfect model but also plants with an imperfect model, which causes uncertainties. We verify the global stability of the proposed method by using Lyapunov method. In order to support the achievement, the application of the proposed adaptive fuzzy regulator to the control of a nonlinear system under the external disturbance is presented and the performance was verified by some simulation result.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.66-71
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• 2016

PikD is a widely known pathway-specific regulator for controlling pikromycin production in Streptomyces venezuelae ATCC 15439, which is a representative of the large ATP-binding regulator of the LuxR family (LAL) in Streptomyces sp. RapH and FkbN also belong to the LAL family of transcriptional regulators, which show greatest homology with the ATP-binding motif and helix-turn-helix DNA-binding motif of PikD. Overexpression of pikD and heterologous expression of rapH and fkbN led to enhanced production of pikromycin by approximately 1.8-, 1.6-, and 1.6-fold in S. venezuelae, respectively. Cross-complementation of rapH and fkbN in the pikD deletion mutant (ΔpikD) restored pikromycin and derived macrolactone production. Overall, these results show that heterologous expression of rapH and fkbN leads to the overproduction of pikromycin and its congeners from the pikromycin biosynthetic pathway in S. venezuelae, and they have the same functionality as the pathwayspecific transcriptional activator for the pikromycin biosynthetic pathway in the ΔpikD strain. These results also show extensive "cross-communication" between pathway-specific regulators of streptomycetes and suggest revision of the current paradigm for pathwayspecific versus global regulation of secondary metabolism in Streptomyces species.

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.138-139
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• 2007

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.160-160
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• 2008

• The Korea Genome Conference
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• 2003.09a
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• pp.40.2-40.2
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• 2003