• 한국식물병리학회지
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• 제14권1호
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• pp.83-91
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• 1998

Gladioli (Gladiolus hybridus) showing flower colour breaking leaf mosaics, notched leaf, and dwarfing or lack of visible symptoms were collected from gladioli growing areas in Taegu and Kyungpook province, Korea. The three viruses isolated from the naturally infected gladioli were identified as broad bean wilt virus (BBWV), cucumber mosaic virus (CMV), and tobacco rattle virus (TRV) by their host range, immunosorbent electron microscopy (ISEM), enzyme-linked immunosorbent assay (ELISA), direct tissue blotting immunoassay (DTBIA), and intracellural symptoms. By DTBIA and ISEM, TRV was detected in gladiolus showing notched leaf, while CMV was frequently detected in gladioli with dwarfing, color breaking and malformation of flowers. BBWV was also often detected in many symptomless gladiolus plants, but TRV was detected in notched-leaf of gladiolus. Electron microcopic examination of negatively stained preparations showed that BBWV and CMV are spherical particles of 28 nm and 30 nm in diameter, and TRV is rigid rod-shaped particles of 40∼200 nm in length. The rigid rodshaped virus particles reacted positively with TRV antiserum in ISEM and DTBIA, respectively.

• 한국식물병리학회지
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• 제14권1호
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• pp.74-82
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• 1998

Gladioli (Gladiolus hybridus) showing flower colour breaking, leaf mosaics, necrotic fleck, and dwarfing or lack of visible symptoms were collected from gladioli growing areas in Taegu and Kyungpook province, Korea. The two viruses isolated from the naturally infected gladioli were identified as ban yellow mosaic virus (BYMV) and clover yellow vein virus (CIYVV) by their host range, immunosorbent electron microscopy (ISEM), enzyme-linked immunosorbent assay (ELISA), direct tissue blotting immunoassay (DTBIA) and intracellural symptoms. In ultrathin sections of BYMV and CIYVV infected tissues, laminated aggregate-type inclusions, cytopalsmic bodies and nuclear inclusions as well as filamentous virus particles were observed in the cytoplasm of parenchyma cells. By DTBIA and ISEM, BYMV was detected in all tested gladiolus plants showing severe or mild mosaic symptoms, whereas CIYVV were mainly detected from those of mild mosaic symptoms. BYMV is the most prevalent in commercial gladioli and present major production problems. Detection sensitivity of BYMV and CIYVV in crude sap of infected gladiolus leaves by ISEM was about twice compared with ELISA. In a comparison of ELISA, ISEM, DTBIA, BYMV was detected in same degree by DTBIA in samples where sap extracts were positive in both ELISA and ISEM. DTBIA provides a specific, rapid, and simple tool for large-scale diagnosis of BYMV.

• 식물병연구
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• 제8권4호
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• pp.207-214
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• 2002

대구시와 경상북도 지역에서 Tobacco rattle virus(TRV) 감염에 의한 증상으로 보이는 글라디올러스(Gladiolus hybridus), 크로커스(Crocus spp.), 수선화(Narcissus spp.)를 채집하였다. 톱니무늬(notch), 줄무늬(stripe), 변형 (malformation)등의 증상의 잎 조직을 시료로 하여 direct negative staining method(DN) 및 immunosorbent electron microscopy(ISEM)법에 의해 투과전자현미경으로 바이러스 입자를 관찰한 결과, 각각의 시료에서 긴 입자는 170~200 X 22nm, 짧은 입자는 40~114$\times$22nm 크기의 막대모양 입자가 다수 확인되었다. 글라디올러스에서 분리한 tobacco rattle virus(TRV-K)를 담배(Nicotiana tabacum)에 즙액 접종, 증식시킨 후, 정제하여 항혈청을 제작하였다. TRV-K 의 coat protein(CP)유전자를 특이적인 올리고뉴클레오타 이드 프라이머를 이용하여 역전사-중합효소연쇄반응(RT-PCR)으로 증폭시킨 후에 염기서열분석법으로 확인하였다. 그 결과 TRV-ORY의 CP와 99.5%의 상동성을 나타냈다.

• 한국응용곤충학회지
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• 제22권3호
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• pp.198-202
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• 1983

그라디오러스에 발생하는 바이러스병을 포장에서 조사한 결과 이 병율이 $43.3\%$이었다. BYMV는 명아주에 엽맥괴사병징, 강남콩에 심한 모자익 병징 그리고 잠두에 모자익 병길을 일으켰다. CMV는 명아주에 국부병반, 담배와 오이에 모자익 병징을 일으켰다. 분류 동정된 두 바이러스는 모두 복숭아 혹진딧물에 의하여 전염되었다. 바이러스를 순화하여 흡광도를 측정한 결과 두 바이러스 모두 260nm에서 최고의 흡광도를 나타내었다. 한천내확산법을 이용한 항혈청과의 반응 결과 SDS를 처리한 BYMY와 CMV는 각각 그 바이러스의 항혈청과 뚜렷한 반응대를 형성하였다. 바이러스 입자의 진자현미경검경 과파 BYMV는 길이가 750nm였으며, CMV는 직경이 30nm이었다.