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http://dx.doi.org/10.5423/RPD.2002.8.4.207

Characterization of Tobacco rattle virus(TRV-K) isolated in Korea  

Shin, Hye-Houng (영남대학교 이과대학 생물학과)
Koo, Bong-Jin (영남대학교 이과대학 생물학과)
Kang, Sang-Gu (영남대학교 생명공학연구소)
Chang, Moo-Ung (영남대학교 이과대학 생물학과)
Ryu, Ki-Hyung (서울여자대학교 환경생명과학부 원예학과)
Publication Information
Research in Plant Disease / v.8, no.4, 2002 , pp. 207-214 More about this Journal
Abstract
Tobacco rattle virus(TRV) was detected from Gladiolus hybridus, Crocus spp. and Narcissus spp. leaves show-ing notched or stripe on the leaf and malformation symptoms collected from Daegu and Kyungbuk province by electron microscopy (EM), immunosorbent electron microscopy (ISEM) and host range study. Direct negative staining method by EM showed rigid rod long particles 170~200$\times$22 nm and rigid rod short particles 40~114$\times$22 m. TRV-K isolated from G. hybridus propagated with Nicotiana tabacum. TRV coat protein(CP) gene was amplified using specific oligonucleotide primer by RT-PCR. Sequence analysis of amplified CP gene showed 99.5％ nucleotide similarity to TRV-ORY.
Keywords
CP-gene sequence; RT-PCR; Tobravirus; Tobacco rattle virus;
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