• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.325-332
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• 1998

In vitro phosphorylation experiments with a cell extract of Streptomyces coelicolor A3(2) M130 in the presence of ${\gamma}-[^32P]$]ATP revealed the presence of multiple phosphorylated proteins, including the AfsR/AfsK kinases which control the biosynthesis of A-factor, actinorhodin, and undecylprodigiosin. Phosphorylation of AfsR by a cell extract as an AfsK source was significantly inhibited by Ser/Thr protein kinase inhibitors, staurosporine and K-252a, at concentrations giving 50% inhibition ($IC_50$) of $1{\mu}M\;and\;0.1{\mu}M$, respectively. Further in vitro experiments with the cell extracts showed that phosphorylation of multiple proteins was inhibited by various protein kinase inhibitors with different inhibitory profiles. Manganese and calcium ions in the reaction mixture also modulate phosphorylation of multiple proteins. Manganese at 10 mM greatly enhanced the phosphorylation and partially circumvented the inhibition caused by staurosporine and K-252a. A calcium-activated protein kinase(s) was little affected by these inhibitors. Herbimycin and radicicol, which are known as tyrosine kinase inhibitors, did not show any significant inhibition of AfsR phosphorylation. Consistent with the in vitro effect of the kinase inhibitors, they inhibited aerial mycelium formation and pigmented antibiotic production on solid media. On the contrary, when assayed in liquid culture, the amount of actinorhodin produced was increased by staurosporine and K-252a and greatly decreased by manganese. All of these data clearly show that the genus Streptomyces possesses several protein kinases of eukaryotic types which are involved in the regulatory network for morphogenesis and secondary metabolism.

• Journal of the Korean Association of Geographic Information Studies
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• 제9권2호
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• pp.11-19
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• 2006

Sewerage is the system that improves the quality of human life and prevents many disasters such as floods. However the investigators in Korea only have been concerned about the sewer system, so the sewage treatment plant stays in the basic level like mapping. For example, only one attribute can be recognized in the linear object. Because of this limitation, it makes difficult to manage the linear attribute regarding to the sewage pipe plan. And it is impossible to control a partial (point type, line type) attribute changes of the linear object. We will therefore present the applicable method for the attribute changes of the linear object like the sewage pipe plans. For this reason, this paper is designed on the basis of Dynamic Segmentation(DS). DS has the advantage of giving the attribute value to the exact place in the linear object. As a result of using DS, the variety environment changes around the sewage pipes are applied to the building sewerage data. This also makes it possible to get a precise estimation for the maximum dirty water amount.

• Culinary science and hospitality research
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• 제2권
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• pp.59-87
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• 1996

This study has been done by giving the guide when development of menu or doing marketing strategy in Hotel Restaurant. All products and service has a regular course from induction in market to disappear, so it shall be come out Products Life Cycle theory. In eating business, under the tendency and existing cycle to change menu by taste of customers, liking variation, change of periodic status (environment), MLC(Menu Life Cycle) shall be advised by application PLC(Products Life Cycle) in the process of induction of some menu first and spreading the same business field and then deletion (disappearance) from menu because of no popular. In Emergence Stage, it shall be tried to inform new menu through Cooking Contest, presentation of new menu, free sampling party and others. In Growth Stage, it shall be changed package menu or set menu. In Maturity Stage, it shall be provided complimentary ticket, gift coupon or discount for the customers with some degree of selling record in order to increase using frequency and selling amount after insure new customers. In Decline Stage, it shall be groped for entry underdeveloped country or less advance nation, but there is no possibility to devote in enterprise, it shall be planned substitute menu development in order to withdraw. By inducting MLC concept, it shall be provided the available informations such as“how could some menu be circulate in some restaurant”“Accordingly do strengthen promotion activities or go to low developed area or overseas, or delete it from menuitems and so on”. In the time of decline some menu, it is judged to the time of plan(preparation) of new menu development. In the broad view, when consideration of the concept of menu life cycle, it shall be possible to know which menu is decayed and which menu is developed newly or grown-up, so it shall be provided the important information to estimate the tendency of changing menu and set-up a menu development plan.

• Proceedings of the IEEK Conference
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• 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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• pp.179-182
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• 2001

The chloride volatilization method for the recovery of zirconium and removal of uranium from zirconium containing metallic wastes formed in spent fuel reprocessing was studied using the simulated alloy waste, i.e. the mixture of Zr foil and UO$_2$/U$_3$O$_{8}$ powder. When the simulated waste was heated to react with chlorine gas at 350- l00$0^{\circ}C$, the zirconium metal changed to volatile ZrCl$_4$showing high volatility ratio (Vzr) of 99%. The amount of volatilized uranium increases at higher temperatures causing lowering of decontamination factor (DF) of uranium. This is thought to be caused by the chlorination of UO$_2$ with ZrCl$_4$vapor. The highest DF value of 12.5 was obtained when the reaction temperature was 35$0^{\circ}C$. Addition of 10 vol.% oxygen gas into chlorine gas was effective for suppressing the volatilization of uranium, while the volatilization ratio of zirconium was decreased to 68% with the addition of 20 vol.% oxygen. In the case of the mixture of Zr foil and U$_3$O$_{8}$, the V value of uranium showed minimum (44%) at 40$0^{\circ}C$ with chlorine gas giving the highest DF value 24.3. When the 10 vol.% oxygen was added to chlorine gas, the V value of zirconium decreased to 82% at $600^{\circ}C$, but almost all the uranium volatilized (Vu=99%), which may be caused by the formation of volatile uranium chlorides under oxidative atmosphere.ere.

• The Korean Journal of Food And Nutrition
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• 제23권1호
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• pp.23-29
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• 2010

Pigment concentrates with violet-red color and sweet taste were obtained from purple-fleshed sweet potato(PFSP) using ethyl alcohol and water. Extract from general potato(GP) were used as a control. The relative stability of PFSP pigment concentrate(PFSPPC) in a storage test over 15 days was confirmed in the order of dark > fluorescence > sun-light irradiation. The relative stability of GP pigment concentrate(GPPC) in a storage test over 15 days was confirmed in the order of sun-light > fluorescence > dark storage. The RRP of PFSPPC was higher than that of GPPC, but the color strength of GPPC was 1/2 that of PFSPPC. Treatment of PFSPPC with aluminum potassium sulfate(0.2~0.3%, w/w) best improved its stability. The improved RRPs of PFSPPC were 45.16~47.31% in sun light irradiation, 55.91~60.22% in fluorescence irradiation, and 76.34~75.97% in dark storage conditions. In substituting aluminum potassium sulfate for chitosan, an amount of 0.2~0.3%(w/w) was suitable, giving similar results in improving pigment stability for all concentrates tested. Also, freeze-dried PFSPPC powder was manufactured as a substitute for dextrin, and also as a substitute for chitosan to the extent of 0.25%(w/w). The results of storage stabilite for freeze-dried PFSPPC and GPPC powder over 15 days, irradiation were, PRRs of 74.47~89.36% and 61.54~76.92%, respectively. The stability improving effect of freeze dried PFSPPC powder was confirmed by the results of storage experiments at various conditions. The use of freeze-dried PFSPPC powder was therefore confirmed to be an effective treatment for general foods.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.15-20
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• 1990

The nutritional status of trace minerals in cattle of Java in Indonesia was investigated by evaluating Cu, Fe, Mo, Zn and Mn concentrations in diets and livers, and Cu and Zn concentrations in blood plasma. Investigations were conducted on Jonggol (West Java), Malang (East Java) and Mojokerto (East Java) in both the rainy and the dry seasons in 1988. In Jonggol, low Cu concentrations in diets showing 7.1 mg/kg in the rainy season and 10.9 mg/kg in the dry season were observed and all plasma samples showed Cu concentrations below the critical level ($0.65{\mu}g/ml$). Thirty percent of the liver samples in Malang and 54% of those in Mojokerto showed lower Cu concentrations than the critical level (75 mg/kg on a dry matter basis). Fe concentrations in diets from the three regions showed a wide variation of values ranging from 249 to 30,000 mg/kg. A large amount of Fe was accumulated in livers from Malang and Mojokerto, giving average concentrations of 498 mg/kg. Zn concentration in diet and plasma samples were close to the borderline and some Zn concentration in these samples showed deficient levels. Mo and Mn concentrations in diets and livers showed normal levels.

• Design & Manufacturing
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• 제13권4호
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• pp.23-27
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• 2019

People's interest in the environmental problems of the Earth is growing as they come to the modern world, and research is being actively conducted on how to protect the environment. As a result, the automobile industry, one of the causes of environmental pollution, is also affected. Therefore, research is being conducted to improve the fuel economy and light weight of cars, development of pollution-free cars such as electric cars, and aluminium materials that are lighter than ordinary steel sheets and easier to recycle are gaining attention. In this experiment, the material was formed to form a form of aluminium and the material reduction rate of the side wall of the foam was tested according to the amount of side wall. The material used in the experiment was A3003-O, which is less plastic than normal steel plates, but has excellent corrosion resistance, plasticity and weldability compared to aluminium materials, but has poor tensile strength. For tensile testing, a certain array of Forming Shapes was molded and the height of the Forming was set to 5mm, and the height of the Forming was 4.7mm, indicating that the difference between the first 5mm Forming and the height was not large. In addition, the material reduction rate was tested by giving 15, 0, and -0.15 teas, respectively, and was found to be valuable as a product only for -0.15.

• Korean journal of food and cookery science
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• 제31권3호
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• pp.296-303
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• 2015

We investigate black raspberry (Rubus occidentalis) wine made using traditional yeast (Saccharomyces cerevisiae A8, B6, GBY2, GBY3) and S. cerevisiae Fermivin (FM), which is widely used in wine manufacturing, and analyze the biogenic amine content and the volatile flavor compounds. Black raspberries were separately inoculated with yeast up to $1{\times}10^9CFU/kg$, followed by incubation at $25^{\circ}C$ for 7 days. FM produced the highest alcohol content, however the final fermentation characteristics of the wine made using four different yeasts were similar. S. cerevisiae A8 had a large biogenic amine (BA) content, specifically tryptamine, thus we excluded this yeast from fermentation. S. cerevisiae GBY3 was selected for black raspberry wine fermentation as a result of sensory evaluation. The volatile flavor compounds of two wines (S. cerevisiae GBY3 and FM) were analyzed by gas chromatography and mass spectrometry. 37 compounds in the samples were separated, and several ester compounds were identified in greater amounts in the wine made with S. cerevisiae GBY3 than in the wine made with FM. A greater amount of the major compound, ethyl benzoate, giving the sweet and fruity flavor, was identified in wine made with S. cerevisiae GBY3 than in the wine made with FM. In conclusion, S. cerevisiae GBY3 was confirmed to produce no major BAs and a better flavored wine. These results give new leads in the production of high quality wine.

• Progress in Superconductivity and Cryogenics
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• 제17권1호
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• pp.10-13
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• 2015

High-temperature superconducting coated conductors (HTS-CCs) are good candidates for resistive superconducting fault current limiter (RSFCL) applications. However, the high current density they can carry and their low thermal diffusivity expose them to the risk of thermal instability. In order to find the best compromise between stability and cost, it is important to study the heat transfer between HTS-CCs and the liquid nitrogen ($LN_2$) bath. This paper presents an experimental method to monitor in real-time the temperature of a quenched HTS-CC during a current pulse. The current and the associated voltage are measured, giving a precise knowledge of the amount of energy dissipated in the tape. These values are compared with an adiabatic numerical thermal model which takes into account heat capacity temperature dependence of the stabilizer and substrate. The result is a precise estimation of the heat transfer to the liquid nitrogen bath at each time step. Measurements were taken on a bare tape and have been repeated using increasing $Kapton^{(R)}$ insulation layers. The different heat exchange regimes can be clearly identified. This experimental method enables us to characterize the recooling process after a quench. Finally, suggestions are done to reduce the temperature increase of the tape, at a rated current and given limitation time, using different thermal insulation thicknesses.

• Journal of the Korean Society of Propulsion Engineers
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• 제17권5호
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• pp.10-17
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• 2013

This paper describes a study on after-burning suppressant in a solid propellant to reduce the plume formed outside of rocket nozzles, which could expose the launch site and the flight track. The minimum smoke propellant to enhance the stealth ability was formulated in terms of the kinds and the effects of after-burning suppressant on the ballistic performance and the amount of primary smoke. A after-burning suppressant, $K_2SO_4$ of about 1.1% weight content was found to show profound reduction of the rocket plume, giving negligibly slight increase in pressure exponent of burning rate. Also minimum smoke propellant with less than 1.1% of $K_2SO_4$ corresponds to A-class satisfaction in primary smoke by AGARD standard.