• Journal of Ginseng Research
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• 제47권1호
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• pp.159-165
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• 2023

Background: Red ginseng marc, the residue of red ginseng left after water extraction, is rich in dietary fiber. Dietary fiber derived from fruits or vegetables can promote the proliferation of probiotics, and it is a key technology in the food industry to increase the productivity of probiotics by adding growth-enhancing substances such as dietary fiber. In this study, the effect of red ginseng dietary fiber (RGDF) on the growth of probiotic bacterial strains was investigated at the phenotypic and genetic levels. Methods: We performed transcriptome profiling of Lactiplantibacillus plantarum IDCC3501 in two phases of culture (logarithmic (L)-phase and stationary (S)-phase) in two culture conditions (with or without RGDF) using RNA-seq. Differentially expressed genes (DEGs) were identified and classified according to Gene Ontology terms. Results: The growth of L.plantarum IDCC3501 was enhanced in medium supplemented with RGDF up to 2%. As a result of DEG analysis, 29 genes were upregulated and 30 were downregulated in the RGDF-treated group in the L-phase. In the S-phase, 57 genes were upregulated and 126 were downregulated in the RGDF-treated group. Among the upregulated genes, 5 were upregulated only in the L-phase, 10 were upregulated only in the S-phase, and 3 were upregulated in both the L- and S-phases. Conclusions: Transcriptome analysis could be a valuable tool for elucidating the molecular mechanisms by which RGDF promotes the proliferation of L.plantarum IDCC3501. This growth-promoting effect of RGDF is important, since RGDF could be used as a prebiotic source without additional chemical or enzymatic processing.

• Journal of Ginseng Research
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• 제8권2호
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• pp.91-104
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• 1984

This study was conducted to investigate the characteristics of nonstarchy polysaccharides in Korean ginseng, (Panax ginseng C.A. Meyer). The results obtained are as follows. 1. The total sugar content of ginseng roots were decreased with increasing the cultural period. On the other hand, the crude fiber content was increased with that of the ginseng leaves or stems. But the crude fiber in root was much less than that of leaves and stems. 2. The dietary fiber content of ginseng root on 5 years old was 14.20% as neutral detergent fiber, 9.08% as acid detergent fiber, hemicellulose 5.12%, cellulose 7.98% and lignin of 1.10%, respectively. 3. Much more pectin was found in ginseng roots which was cultivated for shooter Period. And it was contained much more in the root than in the leaves and stems. 4. ginseng hemicellulose content in root was 5% to 10%. It was decreased with increasing: cultivated period. Hemicellulose was constituted of xylose, arabinose, glucose, rhamnose and xylose of these sugars was the predominant. 5. X-ray diffraction Pattern of ginseng cellulose showed maximum intensity at tile interplanar angle of 4.1$^{\circ}$.

• Journal of Ginseng Research
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• 제22권4호
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• pp.289-293
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• 1998

Six-year-old ginseng roots were divided into rhizome, main root (epidermis, cortex and xylem) and lateral root (big tail root, mid tail root and fine tail root) and the concentration levels of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) in each part of the ginseng were investigated. The amount ratios of SDF to IDF (SDF/IDF) in various parts of the ginseng root were also compared. The concentration levels of SDF and IDF in the ginseng root were 6.56% and 15.41 %, respectively, where the level of SDF in main root was a little higher than that of lateral root. However the amount of IDF in main root was lower than that of lateral root. The SDF/IDF was highest in main root, 0.513, which was higher than that of lateral root or rhizome. The SDF/IDF was 0.704 in xylem, 0.478 in cortex, and 0.099 in epidermis of the main root and the SDF/IDF was 0.576 in big tail root, 0.463 in mid tail root, and 0.255 in fine tail root of the lateral root. It has been reported that SDF might have preventive effects on diabetes, obesity, high blood pressure, colon and rectum cancers, while IDF might have preventive effects on constipation. Therefore, main root of six-year- old ginseng root is thought to have a little different physiological activity from lateral or fine tail roots.

• 한국식품조리과학회지
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• 제8권3호
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• pp.247-253
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• 1992

The total dietary fiber (TDF) contents in cultivated dodok, wild dodok and ginseng were determined by AOAC method and Mongeau et al's method. Also, the nonstarch polysaccharide (NSP) contents of them were analyzed by Englyst et al's gas chromatographic (GC) method. The TDF values by AOAC method and Mongeau et al's method were 34.50% and 35.92% for wild dodok, 46.40% and 47.55% for cultivated dodok, and 14.93% and 14.03% for ginseng, respectively. The ratios of soluble dietary fiber (SDF) contents to TDF contents by Mongesu et al's method were 64.17% for wild dodok, 75.77% for cultivated dodok, and 53.74% for ginseng, respectively. The major sugar constituents of NSF in wild dodok, cultivated dodok and ginseng by GC were glucose, galactose and uronic acid. The Enalyst's TDF contents, i.e., NSP plus lignin contents in wild dodok, cultivated dodok, and ginseng were 20.65%, 20.03% , and 9.72%, respectively.

• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1309-1316
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• 2023

To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains (Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng, using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1997년도 춘계총회 및 학술대회
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• pp.15-15
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• 1997

• Journal of Microbiology and Biotechnology
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• 제34권6호
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• pp.1287-1298
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• 2024

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) that is currently difficult to treat effectively. Both Bacillus natto (BN) and ginseng-soluble dietary fiber (GSDF) are anti-inflammatory and helps sustain the intestinal barrier. In this study, the protective effects and mechanism of the combination of B. natto JLCC513 and ginseng-soluble dietary fiber (BG) in DSS-induced UC mice were investigated. Intervention with BG worked better than taking BN or GSDF separately, as evidenced by improved disease activity index, colon length, and colon injury and significantly reduced the levels of oxidative and inflammatory factors (LPS, ILs, and TNF-α) in UC mice. Further mechanistic study revealed that BG protected the intestinal barrier integrity by maintaining the tight junction proteins (Occludin and Claudin1) and inhibited the LPS/TLR4/NF-κB pathway in UC mice. In addition, BG increased the abundance of beneficial bacteria such as Bacteroides and Turicibacter and reduced the abundance of harmful bacteria such as Allobaculum in the gut microbiota of UC mice. BG also significantly upregulated genes related to linoleic acid metabolism in the gut microbiota. These BG-induced changes in the gut microbiota of mice with UC were significantly correlated with changes in pathological indices. In conclusion, this study demonstrated that BG exerts protective effect against UC by regulating the LPS/TLR4/NF-κB pathway and the structure and metabolic function of gut microbiota. Thus, BG can be potentially used in intestinal health foods to treat UC.

• Applied Biological Chemistry
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• 제36권2호
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• pp.80-85
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• 1993

가공적성을 떨어뜨리지 않으면서 우수한 기능특성을 보이는 식이섬유원은 제조할 목적으로 대량 수급이 용이한 사과쥬스박과 두유박에 작종 이화학적 처리하여 식이성유의 함량과 기능성을 향상시킨 식이섬유원을 이용하여 이들을 직접 식품에 응용하여 그 이용 가능성을 모색하였다. 식이섬유질원을 밀가루와 혼합한 복합분의 호화양상을 Amylograph로 측정한 결과 식이섬유질원의 첨가량이 증가될수록 점도가 감소하였다. 식이섬유질원을 첨가하여 면을 제조하여본 결과 식이섬유질원의 첨가량이 증가할수록 조리면의 중량과 부피는 증가하였으나 인장강도는 감소하였는데 처리 사과육이 처리 두유박보다 인장강도의 감소가 심하였다. 관능검사에서는 처리 두유박 5% 첨가시 밀가루면보다 우수한 관능성을 보였으며 처리 두유박 10%와 처리 사과육 5% 첨가시 향기, 색도, 조직감 및 전체적인 맛에서 밀가루면과 유의차를 보이지 않았다.

• Journal of Ginseng Research
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• 제38권3호
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• pp.203-207
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• 2014

Background: There is limited understanding of the effect of dietary components on the absorption of ginsenosides and their metabolites into the blood. Methods: This study investigated the pharmacokinetics of the ginseng extract and its main constituent ginsenoside Rb1 in rats with or without pretreatment with a prebiotic fiber, NUTRIOSE, by liquid chromatography tandem mass spectrometry. When ginsenoside Rb1 was incubated with rat feces, its main metabolite was ginsenoside Rd. Results: When the intestinal microbiota of rat feces were cultured in vitro, their ginsenoside Rd-forming activities were significantly induced by NUTRIOSE. When ginsenoside Rb1 was orally administered to rats, the maximum plasma concentration (Cmax) and area under the plasma drug concentratione-time curve (AUC) for the main metabolite, ginsenoside Rd, were $72.4{\pm}31.6ng/mL$ and $663.9{\pm}285.3{\mu}g{\cdot}h/mL$, respectively. When the ginseng extract (2,000 mg/kg) was orally administered, Cmax and AUC for ginsenoside Rd were $906.5{\pm}330.2ng/mL$ and $11,377.3{\pm}4,470.2{\mu}g{\cdot}h/mL$, respectively. When ginseng extract was orally administered to rats fed NUTRIOSE containing diets (2.5%, 5%, or 10%), Cmax and AUC were increased in the NUTRIOSE receiving groups in a dose-dependent manner. Conclusion: These findings reveal that intestinal microflora promote metabolic conversion of ginsenoside Rb1 and ginseng extract to ginsenoside Rd and promote its absorption into the blood in rats. Its conversion may be induced by prebiotic diets such as NUTRIOSE.

• 한국식품영양학회지
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• 제23권3호
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• pp.405-410
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• 2010

인삼의 저장 가공 방법을 개발하기 위해 마이크로파 단독처리로 수삼을 처리한 후 일반 성분, 색도, 밀도, 사포닌의 조성 및 미세구조 등 품질 특성을 조사하였다. 마이크로웨이브 출력을 100 Watt와 200 Watt에서 각각 1시간과 3시간 동안 수삼을 처리하고 $60^{\circ}C$ 열풍건조기에서 96시간 건조한 후 성분 함량을 측정하였다. 수삼의 초기 수분 함량인 76.26%에서 마이크로파 가열 처리 조건에 따라 13.12~10.77%까지 건조되었다. 마이크로파로 처리한 수삼의 회분 함량은 처리 과정에 따른 차이는 보이지 않았다. 지방의 경우 마이크로파 출력과는 상관없이 처리 시간에 따라 조금씩 감소하였다. 단백질 함량은 마이크로파 가열 후 건조하기 전 수삼의 단백질 함량과 비교하여 큰 차이를 나타내지 않았다. 마이크로파로 처리한 수삼의 총 식이섬유 함량은 대조군보다 증가함을 보이고 있으나, 처리 시간이나 출력과의 유의성은 보이지 않는다. 처리군의 색도는 대조군에 비해 더 어두운 색을 나타내었다. 마이크로파 가열 처리 후 인삼의 색도의 변화는 출력보다 가열 처리 시간에 따라 변하는 양상을 보였다. 마이크로파 가열 처리 후 수삼의 밀도는 감소하는 경향을 보였고, 가열 시간이나 출력에 따른 뚜렷한 차이는 찾을 수 없었다. 사포닌 ginsenoside-$Rb_1$과 $Rb_2+Rb_3$, Rc, Rd, Re, Rf, $Rg_1$, $Rg_2+Rh_1$, $Rg_3$의 함량은 대조군에 비해 감소하는 경향을 보였고, 100 Watt에서 가열한 처리구들보다 200 Watt에서 가열한 처리구들의 함량이 높은 값을 나타냈다. 예외적으로 200 Watt에서 가열했을 때, ginsenoside-$Rb_2+Rb_3$와 ginsenoside-Rc의 함량은 대조군에 비해 증가하였다. 미세구조는 마이크로파 건조 후 더 치밀한 조직이 관찰되었다.