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Diversity of Endophytic Fungi Isolated from Korean Ginseng Leaves

  • Eo, Ju-Kyeong;Choi, Min-Seok;Eom, Ahn-Heum
    • Mycobiology
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    • v.42 no.2
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    • pp.147-151
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    • 2014
  • We investigated the diversity of the foliar endophytes of Korean ginseng. Endophytic fungi were isolated from healthy leaves of mountain-cultivated ginseng (MCG) and field-cultivated ginseng (FCG) at 4 sites in Chungbuk Province. A total of 24 species of fungal endophytes were identified using molecular approaches. Additionally, the diversity of these endophytic fungi was compared between MCG and FCG. The major isolated endophytes were Edenia gomezpompae and Gibberella moniliformis in the MCG and FCG samples, respectively. The results suggest that ginseng endophytes have different community structures in different environments, and this understanding may prove useful in ginseng cultivation.

Bibliographic consideration on the efficacy and the origin of Korean ginseng (고려인삼의 유래 및 효능의 서지학적 고찰)

  • Kwak, Yi-Seong
    • Journal of Ginseng Culture
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    • v.1
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    • pp.43-56
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    • 2019
  • Korean ginseng (Panax ginseng) has been known as one of the representative special and healthful products originating from Korea for 4500 to 5000 years. The word of ginseng was first mentioned in JiJuZhang(急就章), written by ShiYou during the reign of King Yuah Di of the Chien Han Dynasty, China (33-48 BC). It has been known that wild Korean ginseng grows in Korean peninsula including Manchuria and the ginseng is found only between the $33^{rd}$ and $48^{th}$ parellels of north latitude. Since the times of three kingdom in Korea at 4-7 century, which is Kokuryo, Baekje and Shila, Korea has been the chief ginseng producing country. A large quantity of ginseng was exported from Korea to China for medicinal use at that times. That was written in SamGukSaGi(三國史記) by BuSik Kim of Koryeo Dynasty in Korea in 1145. The cultivation of Korean ginseng was also recorded in Bencaogangmu(本草綱目) written by LiShi Zen during the regin of the Ming Dynasty in 1596, China. The ginseng seedling, which was known as an original method invented by imitating the method of rice transplantation, appeared in the SeungJeongWon Ilgi(the diaries of the royal secretariat, 承政院日記), 1687 in the regin of King SukJong in Korea. It was suggesting that ginseng cultivation was firstly established in the early 1600s in Korea. On the other hand, red ginseng(written as 熟參) was reported firstly in GoRyeoDoGyeong(高麗圖經)(a record of personal experience in Korea, written in 1123) by SeoGung in Song Dynasty, China. The names of Pansam(written as 板蔘) and Pasam(written as 把蔘), which were the another types of red ginseng products, were came on in the JoSeon Dynasty Annals in 1552 and 1602, respectively. Although the term of red ginseng(Hongsam in Korean) was firstly appeared in the JoSeon Dynasty Annals in 1797, it is believed to have been developed a little earlier periods from the King Jungjong(1506~1545) to the King SeonJo(1567~1608) in Korea. Then, the Korean red ginseng has begun production on a large scale in SamJeong Department of NaeJangWon(內藏院 蔘政課) in the Korean Empire(大韓帝國) in 1899. More detailed records about red ginseng production method were written in the SohoDanag Miscellany(韶濩堂集) by Taekyoung Kim at 1916 year in Korea. On the while, the efficacy of ginseng was first recorded in Shennongbencaojing(神農本草經) written in China(BC 83-96) and the efficacy has been continuously inherited.

Analysis of Good Agricultural Practices (GAP) in Panax ginseng C.A. Mayer (인삼의 GAP (우수농산물인증) 관련요소 분석)

  • Yu, Yong-Man;Oh, She-Chan;Sung, Bong-Jae;Kim, Hyun-Ho;Youn, Young-Nam
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.3
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    • pp.220-226
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    • 2007
  • For the analysis of hazard factors and the development of GAP (Good Agricultural Practices) Ginseng, 10 ginseng cultural farms wished certified GAP were selected at Geumsan-gun area, a representative site of ginseng cultivation in Korea. In order to verify the safety of GAP ginseng, possible contamination of pesticide and heavy metal residues, and microbial hazard were analyzed. Soil and water around ginseng cultivation field, and ginseng were investigated. Eighty-one pesticides including carbendazim were used as typical pesticide against plant pathogens and insect pests of ginseng plant and general crops. There was no excess the maximum residue limit (MRL) in residue figure of the soil. Including the residue figure of the arsenic (0.81 ml/kg) and 7 other heavy metals was also suitable to cultivate the ginseng plant. The irrigation water and dilution water for pesticide application were also safety level for GAP. Fresh ginsengs from the farms were sampled and investigated pesticide residues and contaminations of bacteria. Among 23 pesticides tested, we didn't detect any kinds of pesticide residues, but tolclofos-methyl was frequently found in the other ginseng field. On the investigation of microorganism hazards, 2 gram negative bacteria and 1 gram positive bacterium were found in the fresh ginseng. Number of total bacteria was $1.5{\times}10^3$ cfu/ml, which was less than the other agriculture products. At these results, 10 selected ginseng farms were good cultural places for GAP ginseng production and the ginseng cultured from Geumsan-gun area were a good safe far human.

Effect of Green Manure Crop Cultivation on Soil Chemical Properties and Root Rot Disease in Continuous Cropping Field of Ginseng (녹비작물 재배가 토양화학성 및 인삼뿌리썩음병 발생에 미치는 영향)

  • Lee, Sung Woo;Park, Kyung Hoon;Lee, Seung Ho;Jang, In Bok;Jin, Mei Lan
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.1
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    • pp.1-9
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    • 2017
  • Background: Some plants have harmful effects on fungi and bacteria as well as other plants. Incorporating such plant into soil as green manure is effective in reducing population densities of soil pathogens. Methods and Results: Twenty-three species of green manure crops were cultivated after the harvest of 6-year-old ginseng and then incorporated into the soil at the flowering stage. The following year, the root rot ratio of 2-year-old ginseng and soil chemical properties were investigated. In the absence of green manure addition, the $NO_3$ content, electric conductivity (EC), and K content decreased by 95%, 79% and 65%, respectively. In the presence of green manure addition, $P_2O_5$ and $NO_3$ contents reduced by 41% and 25%, respectively. The "survived root ratio" of 2-year-old ginseng significantly increased by 56.2%, 47.5%, and 47.3%, in the Sorghum sudanense, Ricinus communis and Helianthus tuberosus treatment, respectively. In addition, there was a significant increase in the "survived root ratio" in the Secale cereale, Chrysanthemum morifolium, Atractylodes macrocephala, and Smallanthus sonchifolius treatments. The "survived root ratio" of ginseng showed a significant positive correlation with the soil pH and a negative correlation with the $NO_3$ contents, and EC. Conclusions: Cultivation of plant form the Chrysanthemum family as green manure, using mainly the rhizomes was effective for the control of root rot disease of ginseng.

The changes and meanings in the volume of Korea red ginseng trade in late chosun dynasty (조선후기 고려홍삼 무역량의 변동과 의미)

  • Lee, Chulsung
    • Journal of Ginseng Culture
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    • v.1
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    • pp.67-77
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    • 2019
  • This study tried to evaluate the official Korea Red Ginseng(Hongsam) trade in 19th century. The Hongsam trade activities of the 19th century also show that the collected amount of Posam taxes (Hongsam taxes) alone outweigh the amount of cost spent during the same time period to launch tributary actions. It is quite obvious that the Chosun dynasty, supported by the developed techniques of ginseng cultivation and preservation methods, managed to stop the silver leaks while also stimulating the domestic commerce, handicraft business and mining operations, by exporting Hongsam to China and importing raw material and other finished products in return. The Chosun government also managed to secure considerable amount of marginal profit which at times mounted to almost 2 hundred thousand Nyangs of silver during the latter half of the 19th century thanks to this Hongsam trade activities, and accumulated the hoof-shaped pieces of silver ingot at the office of Ministry of Taxation. Even under the mostly undesirable political environment featuring unjustified deeds of the powerful houses, the commercial activities were being strongly maintained, and the transactions conducted by merchants of the Gaeseong, Euiju areas and the capital city were prospering.

Diversity of Yeasts Associated with Panax ginseng

  • Hong, Soon-Gyu;Lee, Kang-Hyun;Kwak, Jang-Yul;Bae, Kyung-Sook
    • Journal of Microbiology
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    • v.44 no.6
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    • pp.674-679
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    • 2006
  • Biodiversity of yeasts was investigated in the ginseng cultivation field. Among 34 isolates tested in this study, 26 isolates belonged to the hymenomycetous yeast group. These 26 strains were classified into 12 species including four new-species candidates that did not have clear affiliation to any established species. Seven isolates among the remaining strains were classified into three ascomycetous yeast species, and one isolate was identified as a urediniomycetous yeast species.

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

  • Park, Young-Hwan;Mishra, Ratnesh Chandra;Yoon, Sunkyung;Kim, Hoki;Park, Changho;Seo, Sang-Tae;Bae, Hanhong
    • Journal of Ginseng Research
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    • v.43 no.3
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    • pp.408-420
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    • 2019
  • Background: Ginseng (Panax ginseng Meyer) is an invaluable medicinal plant containing various bioactive metabolites (e.g., ginsenosides). Owing to its long cultivation period, ginseng is vulnerable to various biotic constraints. Biological control using endophytes is an important alternative to chemical control. Methods: In this study, endophytic Trichoderma citrinoviride PG87, isolated from mountain-cultivated ginseng, was evaluated for biocontrol activity against six major ginseng pathogens. T. citrinoviride exhibited antagonistic activity with mycoparasitism against all ginseng pathogens, with high endo-1,4-${\beta}$-D-glucanase activity. Results: T. citrinoviride inoculation significantly reduced the disease symptoms caused by Botrytis cinerea and Cylindrocarpon destructans and induced ginsenoside biosynthesis in ginseng plants. T. citrinoviride was formulated as dustable powder and granules. The formulated agents also exhibited significant biocontrol activity and induced ginsenosides production in the controlled environment and mountain area. Conclusion: Our results revealed that T. citrinoviride has great potential as a biological control agent and elicitor of ginsenoside production.

A Study of Ginseng Culture within 'Joseonwangjosilok' through Textual Frequency Analysis

  • Mi-Hye Kim
    • CELLMED
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    • v.14 no.2
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    • pp.2.1-2.10
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    • 2024
  • Through big data analysis of the 'Joseonwangjosilok', this study examines the perception of ginseng among the ruling class and its utilization during the Joseon era. It aims to provide foundational data for the development of ginseng into a high-value cultural commodity. The focus of this research, the Joseonwangjosilok, comprises 1,968 volumes in 948 books, spanning a record of 518 years. Data was collected through web crawling on the website of the National Institute of Korean History, followed by frequency analysis of significant words. To assess the interest in ginseng across the reigns of 27 kings during the Joseon era, ginseng frequency records were adjusted based on years in power and the number of articles, creating an interest index for comparative rankings across reigns. Analysis revealed higher interest in ginseng during the reigns of King Jeongjo and King Yeongjo in the 18th century, King Sunjo in the 19th century, King Sejong in the 15th century, King Sukjong in the 17th century, and King Gojong in the 19th century. Examining the temporal emergence and changes in ginseng during the Joseon era, general ginseng types like insam and sansam had the highest frequency in the 15th century. It appears that Korea adeptly utilized ceremonial goods in diplomatic relations with China and Japan, meeting the demand for ginseng from their royal and aristocratic societies. Processed ginseng varieties such as hongsam and posam, along with traded and taxed ginseng, showed peak frequency in the 18th century. This coincided with increased cultivation, allowing a higher supply and fostering the development of ginseng processing technologies like hongsam.