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Variation in Physicochemical Characteristics and Antioxidant Activities of Small Redbean Cultivars (팥 품종의 이화학적 특성 및 항산화 활성 변이)

  • Sung, Jung Sook;Song, Seok Bo;Kim, Ji Young;An, Yeon Ju;Park, Jae Eun;Choe, Myeong Eun;Chu, Ji Ho;Ha, Tae Joung;Han, Sang Ik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.3
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    • pp.231-240
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    • 2020
  • This research was conducted to evaluate the physicochemical properties, antioxidant components, and their activities for more taking advantage of small redbean cultivars. Seed size, 100 seeds weight, and hardness on the 8 cultivars were measured. The free sugar and crude protein contents were evaluated using HPLC and protein analyzer, respectively. Amylose content, antioxidant components, and activities were analyzed by spectrophotometer. The range of 100 seeds weight and hardness were 12.55-18.81 g and 9,527.38-14,341.25 gf, respectively. Total free sugar, amylose, and crude protein were showed 22.49-31.07 mg/g, 13.53-15.67%, and 21.27-23.30%, respectively. The cultivar 'Hongeon' was higher antioxidant component and activity more than others. In clustering the cultivars based on the results, the tree showed four major clades. The 'Huinnarae' group was high in total free sugar and amylose content. The 'Hongeon' group were high in 100 seeds weight, antioxidant component. and activity, while amylose content was lower than that in the other groups. The results of the cultivars can be utilized for research of functional materials. The findings of this study will provide valuable information for expansion of functional food industry related on small redbean.

Evaluation to Purification Capacity of Pollutants by Column Test with the tidal flat sediment (통수실험에 의한 갯벌의 오염물질 정화능력 평가)

  • Kim, Jong-Gu;You, Sun-Jae
    • Journal of Environmental Science International
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    • v.9 no.3
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    • pp.223-228
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    • 2000
  • The purpose of this study is to evaluate the purification capacity of pollutants by column test with the tidal flat sediment. Sediment materials were taken from Chunjangdae tidal flat which located in Chungnam Seochungun. The column tests were conducted on four conditions(R1 : raw sewage filtered by G2 filter, R2 : sterilized sewage after filtered by GF/C filter, R3 : R2 /filtered(membrane) seawater (1:1), R4 : R2 /filtered(membrane) seawater(1:2)). The results of this study may be summarized as followed ; The removed COD by column tests were increased according to increasing the quantity of sewage. During the column tests of 580min, the total removed ammonia nitrogen were 90.1mg for R1, 81.0mg for R2, 27.6mg for R3 and 4.1mg for R4. The result was similar to COD experiment. During the 580min, the total removed total-phosphates were 3.4mg for R1, 4.2mg for R2, 5.6mg for R3 and 2.0mg for R4. The removal efficiency of Pb and Cd for R3 and R4 reactor were higher than R1 and R2 reactor. The remove of heavy metal by the column test was high in sample with seawater. But in the initial 20min, the adsorbed Pb and Cd showed about 3% of the total adsorbed Pb and Cd during 580min.

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Experimental Study for the Development of the Mixing Ratio as a Compaction Pile (다짐말뚝 재료로서 쇄석과 저회의 적정 혼합비 도출을 위한 실험적 연구)

  • Leem, Hansoo;Kim, Sunkon;Lee, Jooho;Chun, Byungsik
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.9
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    • pp.5-16
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    • 2012
  • In the case of using the soil materials created by cutting in-situ ground directly without adjusting particle size, it is recommendable to seek the compaction property or material constant required for filling design or density control through indoor test, and many studies on this subject have been carried out during that time. The researches conducted during that time, however, were focused on the mixed materials with different diameters that exist in a natural condition. There has been no study conducted using coal fly ash that is by-product of the thermal power plant that is actively considered as the building materials. Therefore, this study was aimed at implementing compaction test and examining the basic engineering property in order to explore the influence of crushing the particles through compacting the admixture of crushed stone and coal fly ash produced from thermal power plant on its engineering property, and then the impact of the admixture volume of each material on compaction property and material property by conducting the One-Dimensional Compression Test. As result of compaction test, the optimum moisture ratio of coal fly ash was shown to be approx. 23%. As result of compaction test in accordance with the mixed ratio of coal fly ash and crushed stone under the same compaction energy and moisture ratio, dry unit weight tended to drop when the mixed ratio of coal fly ash exceeded 30%, while it reached approx. $1.81gf/cm^3$ when the mixed ratio was 30%. As result of One-Dimensional Compression Test in accordance with the mixed ratio of crushed stone and coal fly ash, the change in void ratio by particle crushing was at the highest level in the case of coal fly ash 100%, while the lowest level in the case of crushed stone 100%. In the case of mixed materials of crushed stone and coal fly ash, compression index was at the lowest level in case of coal fly ash 30%, and therefore this ratio of mixed material was judged to be the most stable from an engineering aspect.

Physical Properties of the Hydrogel Using Alginate (Alginate가 포함된 하이드로겔의 물리적 특성)

  • Woo, Chul-min;Heo, Seongin;Lee, Hyun Mee
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.4
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    • pp.463-469
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    • 2015
  • Purpose: A hydrogel including alginate and $CaCl_2$ extracted from seaweed was manufactured, and their physical properties were investigated. Also, its applicability as contact lenses was examined. Methods: A film-type sample used in this experiment was manufactured using 2-hydroxyethyl methacrylate (HEMA), which is the raw material of hydrogel contact lenses; azobisiobutyonitile (AIBN), which is an initiator and ethylenglycoldimethacrylate (EGDMA), which is a cross-linking agent. It was hydrated in a PBS solution for 24 hours, and an interpenetrating polymer network (IPN) was formed in 1% and 2% alginate and 1%, 3%, and 5% $CaCl_2$ solutions for 24 hours, respectively. Results: The measurement of the physical properties of the film after the IPN showed that the moisture content was 30.89~36.89%, the refractive index was 1.431~1.441, the contact angle was $62.98{\sim}80.45^{\circ}$, and the tensile strength was 2.378~4.215 ($gf/mm^2$). Also, the physical properties hardly changed as the content of alginate increased, and the moisture content decreased as the content of $CaCl_2$ increased. As a result of the IPN, the moisture content and contact angle decreased compared to those of basic HEMA, but the tensile strength increased. The tensile strength of the second IPN was higher than that of the first IPN. In the case of $CaCl_2$, for the sample polymerized for 24 hours and the second IPN sample with 2% alginate, the contact angle decreased as the content of $CaCl_2$ increased. Conclusions: In this study, the tensile strength increased as the content of $CaCl_2$ increased, and the wettability increased as a result of IPN of alginate and $CaCl_2$. The hydrogel containing the alginate and $CaCl_2$ was confirmed possible utilization as contact lens material.

Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide (인간치수세포에 Mineral Trioxide Aggregate와 수산화칼슘 제재 적용 시 유전자 발현 양상 비교)

  • Kim, Yong-Beom;Shon, Won-Jun;Lee, Woo-Cheol;Kum, Kee-Yeon;Baek, Seung-Ho;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.36 no.5
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    • pp.397-408
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    • 2011
  • Objectives: This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs). Materials and Methods: wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction. Results: Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal. Conclusions: Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.

Effect of Solar Salt on Kimchi Fermentation during Long-term Storage (김치제조시 사용되는 천일염이 김치의 장기저장에 미치는 영향)

  • Chang, Ji Yoon;Kim, In Cheol;Chang, Hae Choon
    • Korean Journal of Food Science and Technology
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    • v.46 no.4
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    • pp.456-464
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    • 2014
  • Kimchi was prepared with three types of salt (4-year-aged solar salt, FS; 1-year-aged solar salt, OS; and purified salt, PS), using Leuconostoc citreum GJ7 as the starter culture. The prepared kimchi was fermented (up to 0.5-0.6% of acidity) and stored for 5 months at $-1^{\circ}C$. During the storage period, the acidity of FS kimchi increased gradually, whereas that of PS kimchi increased sharply. The yellowness (b) color value of PS kimchi (63.4) was higher than that of other kimchis with solar salts (55.6-60.3). Hardness of FS kimchi (1,912.6 gf) was greater than that of the other kimchis (1,554.4-1,650.2 gf) during the storage period. Moreover, sensory evaluation showed higher scores for FS kimchi than for other kimchis. These results suggest that FS is more suitable salt than PS for long-term storage of kimchi.

Establishments of Lead Standards through Monitoring Heavy Metals in Calcium, Chitosan, and Propolis Health Foods (칼슘, 키토산, 프로폴리스 건강보조식품중 중금속 모니터링을 통한 납기준 제정)

  • Kim, Mee-Hye;Chung, So-Young;Sho, You-Sub;Kim, Myung-Chul;Kim, Chang-Min
    • Korean Journal of Food Science and Technology
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    • v.33 no.5
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    • pp.525-528
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    • 2001
  • This study was conducted to estimate the contents of heavy metals in some health foods available on Korean markets. The samples were digested with microwave system, then analyzed using GF-AAS for the contents of lead (Pb), cadmium (Cd) and arsenic (As). The contents of mercury (Hg) were determined using a mercury analyzer. The average values of Hg, Pb, Cd and As in calcium (Ca) health foods were 0.007, 1.08, 0.02 and 0.48 mg/kg respectively. Those values in chitosan health foods were 0.001, 0.36, 0.01 and 0.03 mg/kg respectively. Those values in propolis health foods were 0.013, 4.96, 0.01 and 0.13 mg/kg, respectively. The health foods that contained cow bone powders had the highest lead contents. Based on the variation in lead contents of those products, it could be possible that they might be contaminated through raw materials and/or manufacuring process. Some propolis products were also very high in lead contents. There could be risks for some population, especially the aged who overtake those health foods, to have heavy intake of lead. Therefore, we established the lead standards of 3.0, 2.0 and 5.0mg/kg less than for Ca, chitosan and propolis health foods respectively, based on the Codex method.

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Analytical Method for Sodium Polyacrylate in Processed Food Products by Using Size-exclusion Chromatography (Size-exclusion Chromatography를 활용한 가공식품 중 폴리아크릴산나트륨 분석법 확립)

  • Jeong, Eun-Jeong;Choi, Yoo-Jeong;Lee, Gunyoung;Yun, Sang Soon;Lim, Ho Soo;Kim, MeeKyung;Kim, Yong-Suk
    • Journal of Food Hygiene and Safety
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    • v.33 no.6
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    • pp.466-473
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    • 2018
  • An analytical method of sodium polyacrylate in processed food products was developed and monitored by using size-exclusion chromatography. GF-7M HQ column and UV/VIS detector were selected based on peak shape and linearity. Flow rate, column oven temperature, and mobile phase were selected as 0.6 mL/min, $45^{\circ}C$, and 50 mM sodium phosphate buffer of pH 9.0, respectively. Samples for analysis of sodium polyacrylate were extracted with 50 mM sodium phosphate buffer of pH 7.0 for 3 hr at $20^{\circ}C$ and 150 rpm. Analytical method validation revealed proper selectivity and calibration curve was selected in the range of 50-500 mg/L, and correlation coefficient of calibration curve was more than 0.9985. Limit of detection of sodium polyacrylate was 10.95 mg/kg and limit of quantification was 33.19 mg/kg. Accuracy and coefficient of variation for sodium polyacrylate analysis was 99.6-127.6%, 3.0-8.3% for intra-day and 94.3-121.9%, 1.3-2.6% for inter-day, respectively. Sodium polyacrylate was detected in 40 samples among monitored 125 processed food products. Detected contents were less than 0.2%, limited by the Food Additives Code. Results suggest the established size-exclusion chromatography method could be used to analyze sodium polyacrylate in processed food products.

Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study

  • Dagna, Alberto;El Abed, Rashid;Hussain, Sameeha;Abu-Tahun, Ibrahim H;Visai, Livia;Bertoglio, Federico;Bosco, Floriana;Beltrami, Riccardo;Poggio, Claudio;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.42 no.4
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    • pp.316-323
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    • 2017
  • Objectives: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. Materials and Methods: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU). Results: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria. Conclusions: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.

Determination of the Nutritive Value of Tropical Biomass Products as Dietary Ingredients for Monogastrics Using Rats: 1. Comparison of Eight Forage Species at Two Levels of Inclusion in Relation to a Casein Diet

  • Phuc, Bui Huy Nhu;Lindberg, Jan Erik;Ogle, Brian;Thomke, Sigvard
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.986-993
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    • 2001
  • In balance experiments with rats either 25 or 50% of the casein protein in the control diet was replaced with one of the following eight sun-dried tropical biomass products: water spinach plants (WS) (Ipomoea aquatica Forsk), leucaena leaves (LL) (Leuceana leucocephala), duckweed plants (DW) (Lemma minor L.), groundnut foliage (OF) (Arachis hypogaea L.), trichantera leaves (Tric) (Trichantera gigantea), indicago leaves (Ind) (Indigofera hirsuta), mungbean foliage (Mb) (Phaseolus aureus), and cassava leaves (CL) (Manihot esculenta Crantz). The experiment included 102 rats with six individuals per treatment group. In three of the 16 biomass treatment groups, feed intake and weight gain of the rats were unacceptably low, and therefore they were excluded from the statistical evaluation, The crude protein (CP) content of the biomass products varied between 20.9% (Tric) and 33.2% (DW), whereas the content of NDF varied between 18.5% (Ind) and 32.2% (DW) of dry matter (DM). The total content of essential amino acids (g/16 g N) was comparable with that of alfalfa meal, except for GF and Tric, which were inferior. Between plant species, differences in dietary digestibility of organic matter (dOM) and CP (dCP) were observed (p<0.001). Also, the replacement level negatively influenced dOM and dCP (p<0.001). The lowest values for dOM (p<0.001) were observed for diets including biomass products with the highest content of NDF (OF, Tric, Mb, LL). Digestibility of CP was negatively affected by level of protein replacement. Significant (p<0.001) differences were found in N-retention and biological value among diets with different biomass products. The most favourable overall results were obtained for DW, WS and CL. The main factors affecting the nutritive value of the diets tested were their NDF content, dCP and AA profile of the biomass. Also antinutritive component(s) may have influenced the process of digestion and metabolism of some of the biomass products.