• Title/Summary/Keyword: germination ratio

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Allelopathic Inhibition by Extracts and Volatiles from Leaf and Seed of Sicklepod (Cassia tora L.) (결명자(決明子)(Cassia tora L.) 엽(葉)과 종자(種子)의 추출물(抽出物)과 휘발성분(揮發成分)의 타감성(他感性) 생육저해작용(生育沮害作用))

  • Lim, Sun-Uk;Kim, Geum-Sook;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.2
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    • pp.160-167
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    • 1992
  • The growth of weeds and some other plants has been considered to be inhibited by sicklepod(Cassia tora L.) sharing the habitat. The study was conducted, for the first time, to propose that this phenomenon is not only due to competition for physical and nutritional conditions but also due to allelopathy. In addition, autotoxicity of sicklepod was examined. The results are summarized as follows. 1. Rice sheath length reduced progressively from 18 % to 36 % with increasing the concentration of treating aqueous extracts of sickle pod seeds, but rice germination was not affected. In contrast, radish hypocotyl length was not reduced by the aqueous extract treatment but radish germination was significantly reduced by 66 % at 1 : 10 and 1 : 5 treatment. 2. Total chlorophyll contents in rice seedling decreased from 50 % to 65 % by treatment of seed aqueous extracts diluted from 1 : 50 to 1 : 5 ratio. 3. Aqueous extracts of sicklepod leaves significantly reduced hypocotyl length and fresh weight in radish and germination in rice, but mung bean was slightly affected by aqueous extracts only in fresh weight. 4. Volatiles from fresh, immature seeds with husk reduced the radish germination and seedling growth and radish root growth appeared to be more sensitive to the exposure to volatiles from fresh immature seeds than both germination and hypocotyl growth. 5. Volatiles from sickepod leaves inhibited germination and growth of radish, rice and mung bean, and seedling growth was more sensitive to volatiles from leaves than germination. 6. Volatiles from sicklepod leaves reduced germination and radicle length of sicklepod itself. 7. Collectively, it is concluded that there are water-soluble and volatile substances responsible for allelopathy in sicklepod.

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Effects of Seed Size and Several Factors on Ultra-drying and Germination of Ultra-dried Seeds in Soybean

  • Lee, Yeong-Ho;Chang, Ching-huan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.305-309
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    • 2000
  • Ultra-drying [<5.0% seed moisture content (SMC)] storage technique is a cost-effective storage method for oily seeds. To decide proper ultra-drying condition for soybean seeds, drying rate was compared three silica gel to seed ratios, two seed sizes with varietal difference, two kinds of container, and three seed amounts per container under :t 23$\pm$1$^{\circ}C$. When the relative humidity (RH) was reduced at the rate of less than 0.1 % a day, silica gel was replaced with dry one by 47 days. Higher silica gel to seed ratios (3:1 and 2:1) dried faster than lower ratio (1:1) until 28 days, but not after 43 days of drying. Also, large seeded variety was dried faster than small seeded variety. Kinds of container and seed amounts per container didn't show differences in drying of soybean seeds. After completion of ultra-drying, percentage germination by standard germination test (SGT) was not different among silica gel to seed ratios, kinds of container, and seed amounts per container, except among seed sizes (varieties). Before SGT, soybean seeds were premoistened using saturated ${CaCl}_2$ for 48 hours and ${NH}_4$Cl for 24 hours in desiccators. To compare germinability between ordinary-dried seeds and ultra-dried seeds, the seeds of seven soybean varieties, which were varying in size from 8.1 to 34.9 g per 100 seeds, were dried using same amount of silica gel under 23$\pm$1$^{\circ}C$. After completion of 76 days of drying, SMCs were reduced to 3.13-3.45% from 7.86-8.82%. SMC after completion of drying was not correlated with 100-seed weight (r=0.556). Before germination tests, soybean seeds were premoistened using saturated salt solutions. Percentage germination was higher with ultra-dried seeds than ordinary-dried seeds in SGT and higher with ordinary-dried seeds than ultra-dried seeds in AAT at the beginning of storage and after 6 months storage, but general trend of percentage germination was not observed among varieties classified by 100-seed weight. From these results, we concluded that further studies are needed to improve ultra-drying storage method for soybean seeds.

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Allelopathic Effects of Extracts of Trifolium repens on the Seed Germination and Seedling Growth of Zoysia japonica (토끼풀(Trifolium repens) 추출액이 잔디(Zoysia japonica)의 발아와 생장에 미치는 알레로파시 효과)

  • Lee, Ji-Hun;Kim, In-Taek;Lee, Ho-Jun;Kim, Yong-Ok
    • The Korean Journal of Ecology
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    • v.24 no.3
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    • pp.125-130
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    • 2001
  • The allelopathic effects of aqueous extracts from Trifolium repens were studied. Aqueous extracts of leaves, stems, roots and flowers of T. repens strongly inhibited the seed germination and seedling growth of Zoysia japonica. In general, the higher concentration of the extracts showed the more great inhibition effect. The phenolic compounds extracted from T repens were analysed and identified using gas chromatography. 14 phenolic compounds were isolated from the leaves, stems, roots and flowers: caffeic acid, p-hydroxy benzoic acid, ferulic acid, gallic acid, p-coumaric acid, vanillic acid, trans-cinnamic acid, 2,5 dihydroxy benzoic acid, syringic acid, 2-hydroxy cinnamic acid, benzoic acid, salicylic acid, phloroglucinol and phanylacetic acid. The seed germination and relative growth ratio of Z. japonica by 14 phenolic compounds were inhibited in whole treatment. Stronger growth inhibitor were benzoic acid, salicylic acid and trans-cinnamic acid. From these results it is suggested that the phenolic compounds from T. repens seemed to be responsible for the allelopathic potential.

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Germination and Seedling Induction of Viscum album var. coloratum (Kom.) Ohwi after Artificial Inoculation on the Branch of Host Plants (겨우살이의 종자의 기주목 접종 및 유묘 활착기술)

  • Kim, Chul-Woo;Yi, Jae-Seon
    • Journal of Forest and Environmental Science
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    • v.29 no.2
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    • pp.173-180
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    • 2013
  • Berries of Korean mistletoe (Viscum album var. coloratum [Kom.] Ohwi) contained one seed, which have, in general, one or two embryos but very rarely three embryos. Mucilaginous substances in berries may help them adhere to the branches of host trees. It was observed that seeds need more than one and half years to develop into normal and healthy seedlings from the time of inoculation. Many factors such as adhesion of berry, thickness of host branch, orientation of haustorial root, etc. influenced the successful development of mistletoe plants. Through the application of six-year observation results on the germination of seeds and growth of seedlings, about 80% of germination rate for mistletoe seeds and 61% of survival ratio for germinated seeds, which is more than 23 times higher in natural conditions, were obtained after inoculation of seeds on the one-year-old branches of Malus pumila var. dulcissima and Quercus mongolica trees. The technological aspects of the success can be applied to other host plants and provide a critical clue to an artificial propagation system, for this medicinally valuable genus. This is the first successful report on artificial inoculation and plant development of Korean mistletoe.

Several Factors Affecting Mass Production of Microlepia strigosa (Thunb.) C. Presl Sporophytes (돌토끼고사리 포자체의 대량생산을 위한 몇 가지 요인)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Horticultural Science & Technology
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    • v.35 no.1
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    • pp.46-58
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    • 2017
  • This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation and seedling growth in Microlepia strigosa (Thunb.) C. Presl. Spore germination and prothallus development were promoted by low concentrations of Knop medium nutrient solution. The optimal medium for prothallus propagation and antheridium formation was 2X MS medium with 3% sucrose. The activated charcoal content of the medium did not affect prothallus proliferation. Among the various combinations of culture soil (bedding soil, peat moss, perlite and decomposed granite), a mixture of bedding soil, peat moss and decomposed granite at a ratio of 1 : 1 : 1 (v : v : v) had a positive effect on sporophyte formation. The most efficient conditions for promoting the growth of whole plants (sporophyte seedlings) were 50 - cell plug trays filled with a mixture of bedding soil and decomposed granite at a 2 : 1 (v : v) ratio.

Population Dynamics of Arisaema robustum (넓은잎천남성 (Arisaema robustum) 개체군의 동태)

  • 민병미;유진숙
    • The Korean Journal of Ecology
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    • v.21 no.1
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    • pp.27-33
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    • 1998
  • Arisaema robustum, which has the ability to change sex, was studied in a temperate broadleaf forest of Sanseong-ri, Joongbu-myeon, Gwangju-gun, Kyonggi Province, Korea. \ulcornerThe study, carried out from 1993 to 1997, focused on population dynamics energy budget among organs, size distribution, mortality, the relationships between sex and size, seed production and germination rate. In terms of energy budget among the organs, the ratio of aboveground to belowground biomass was 36.6 : 63.4 in non-female plants, and 81.4 : 18.6 in female plants. Also, in female plants, the ration of leaf to sexual organ biomass was 39.5 : 41.9. Therefore, the belowground ratio of female plants was lower than that of non-female plants. Plants were classified into 8 levels relative to the amount of leaf area by $100cm^2$. The rates of the smallest and the largest classes were 49% and 1%, respectively, and population distribution by size was relatively stable. The mortality averaged 13.1% per year and decreased in inverse proportion to leaf size (6.6% in the smallest and 0.0% in the largest size classes). Leaf areas were $64.1{\pm}48.5cm^2$ in non-flowering plants, $232.1{\pm}123.9cm^2$ in males and $444.8{\pm}153.9cm^2$ in females. The increase rates of leaf area per year varied from 1.9% in plants changing from female tomale, to 152.4% in plants changing from non-flowering to female. But plants which remained female for 2 years showed a decrease of 34.7%. >From this result, it is thought that the female plants invest more energy to reproduction than to vegetative organs. The correlation coefficient (CC) value between plant size and the number of seeds produced (0.55) was larger than the CC value between plant size and total seed weight (0.73). That is, the larger the plant size, the heavier the seed produced. The germination rate increased along with seed weight, and it was 95% in plants which were over 60mg fresh weight/seed.

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Planting Properties of Herbaceous Plant and Cool-season Grass in Environmentally Friendly Planting Block Using CSG Materials (CSG 재료를 이용한 친환경 식생 블록 내 초본식물 및 한지형 잔디의 식생 특성)

  • Kim, Young-Ik;Yeon, Kyu-Seok;Kim, Yong-Seong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.52 no.1
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    • pp.33-40
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    • 2010
  • The cemented sand and gravel (CSG) method is a construction technique that adds cement and water to rock-like materials, such as rivered gravel or excavation muck which that can be obtained easily at areas adjacent to dam sites. This study was performed to evaluate the planting properties of herbaceous plant and cool-season grass in CSG blocks that were manufactured by using CSG materials to develop environmentally friendly CSG method. The two types of CSG-0 without cement and CSG-100 with $100\;kg/m^3$ of cement were designed to evaluate compaction, unconfined compressive strength and growth of plants with cement content by using modified E compaction. To analyze growth properties of plants, germination ratio, visual cover, plant height and root length were measured in 4 weeks and 8 weeks after sowing. As the results, the germination regardless kinds of plants started within 5~7days and the germination ratio were in the range of 50~60 %. The visual cover of kinds of plants by visual rating system were in the range of 7~8 and the visual cover of tall fescue and perennial ryegrass was higher than that of lespedeza cuneata. The plant height and root length for tall fescue and perennial ryegrass in 8 weeks after sowing were in the range of 22~26 cm, 12~15 cm and 4~6 cm, 3~5 cm, respectively.

Evaluation of Compost Maturity by Physico-chemical Properties and Germination Index of Livestock Manure Compost (축분 퇴비의 이화학적 특성과 발아지수를 이용한 부숙도 평가)

  • Chang, Ki-Woon;Hong, Joo-Hwa;Lee, Jong-Jin;Han, Ki-Pil;Kim, Nam-Cheon
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.2
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    • pp.137-142
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    • 2008
  • This study was conducted to evaluate the humification grade of compost, based on physico-chemical property and phyto-toxicity during the composting with three kinds of livestock manures and saw dust. The ratios of the compost, which was mixed with pig manure(P) and sawdust(S) were 4 : 6(PS-1), 5 : 5(PS-2) and 6 : 4(PS-3); poultry manure(PO) and sawdust(S) were 4 : 6(POS-1), 5 : 5(POS-2) and 6 : 4(POS-3); cow manure(C) and sawdust(S) were 4 : 6(CS-1), 5 : 5(CS-2) and 6 : 4(CS-3) by volume to volume, and they were decomposed for 60 days. In the result, the temperature in all treatments during composting rapidly increased above $65.4^{\circ}C$, and then gradually decreased to around $40^{\circ}C$. At 60 day, after the treatment, pH 5.9 ~ 8.0 at the incipient stage increased to 7.6 ~ 8.5, and the C/N ratio was 13.8 ~ 21.1 at the final composting stage. Germination Index(G.I.) showed in the range of 75.1 ~ 94.6 in all treatments at day 60. Therefore, it is likely recommended to take the best humification grade when the temperature maintains above $65^{\circ}C$ longer than a day at minimum, in the range of pH 6.5 ~ 8.5 for the final compost, under 20 of C/N ratio, and G.I. above 80. The level of G.I. above 80 should be the mature compost which could be used in the field without gas demage to crops.

Physicochemical Characteristics of Lipoxygenase - Deficient Soybeans (Lipoxygenase 결핍 콩의 이화학적 특성)

  • Kim, Soo-Hee;Hwang, In-Kyeong
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.751-758
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    • 1998
  • Lipoxygenase-deficient soybeans, Jinpumkong (lipoxygenase-2, 3 lacking) and Jinpumkong 2 (lipoxygenase-1, 2, 3 lacking), were breeded for the improvement of beany flavor problem. The objectives of this study were to characterize and to examine the storage stability of two lipoxygenase-deficient soybeans by comparing with Hwangkeumkong having high lipoxygenase activity. The crude protein and crude lipid content of Jinpumkong 2 were lower than those of Hwkangkeumkong and Jimpumkong. All soybean samples were middle-sized and yellow-coated seeds. The rate of water uptake and trypsin inhibitor activity of Jinpumkong were greater than those of others. The cooking rate of Hwangkeumkong was the highest among all. The lipoxygenase activity of Hwangkeumkong was decreased when the soybeans were stored at $40^{\circ}C$ for 96hrs at 90% RH which is the condition of accelerated aging. After accelerated aging, the germination ratio of Hwangkeumkong was not changed but the ratio and speed of germination dropped rapidly in Jinpumkong and Jinpumkong 2.

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Effect of Solid Matrix Priming on Germinability of Seeds of Peppers(Capsicum annuum L.) I. Duration and Temperature (Solid Matrix Priming 처리가 고추종자의 발아에 미치는 영향 I. 처리기간 및 온도의 영향)

  • Kim, Do-Hahn;Jeong, Sung-Woo
    • Journal of agriculture & life science
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    • v.43 no.5
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    • pp.1-8
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    • 2009
  • Treatment duration of 12 pepper species by 3, 5, 7 days examined result, optimum processor of 12 pepper cultivars and carrier : water proportion was difference in species. However, radicle protrusion during priming(RPDP) does not happened and heightens germination rate, and Wang and Manitta species were 1:3(ratio), 7 day by treated that can shorten $T_{50}$, and all of the 10 cultivars were regard to processing of 1:7(ratio), 3 days was make satisfactory progress. Also cultivars of Jongggip was investigated by cultivars which germination rate and $T_{50}$ according to treatment duration and water proportion was irregular tendency than other cultivars. It was suggested that $20^{\circ}C$ was stable to radical protrusion and improved germination, while from $15^{\circ}C$ to $35^{\circ}C$ examined.