• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.47-51
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• 2008

This study was carried out to get the basic data for safflower cultivation according to sowing date by growth analysis for 2 years form 2003 to 2004 at Institute of natural product in Uiseong, G.B.A.R.E.S.. Safflower(Cheongsoo cultivar) was seeded at March 1st, April 1st, May 1st, and July 1st in field. The necessary date for germination was 24 days, when safflower was seeded at March 1st, but it was 8 days which sowed at April 1st and when the seed was sowed at high temperature period, the necessary date for germination was getting short. The necessary date for flowering was the same trend as germination; it took 104 days at March 1st, 79 days at April 1st, 65 days at May 1st, and 58 days at July 1st sowing treatment. The safflower growth was the most vigor when it was sowed at March 1st. T/R(Top/Root) ratio was gently increased during growing season, but when the seed was sowed at July 1 st, it was low compare to sowing at March 1st and April 1st. RGR(Relative growth rate) was the highest during shoot growth stage, but it was rapidly decreased after flowering. CGR(Crop growth rate) was increased at branching stage, and the increasing velocity was slightly decreased just before flowering, and it was increased again at flowering and at this stage, CGR was the highest, and then it did not increased. NAR(Net assimilation rate) was the highest at shoot growth stage during growing season, and was the highest which was sowed at March 1st.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.382-388
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• 1994

For the comparision of the differences in dry matter weight and the chemical composition of soybean seeds in relation to utilization styles and storage durations, the seed coats and cotyledons including embryo axis separated from the seeds of 3 recommended cultivars stored for 3 years and of 5 recommended cultivars and 2 local varieties with black seed coat and green cotyledons were observed in dry weight, germination percentage, protein, oil, soluble sugar, oilgosaccharide, and starch. In the seeds stored for 3 years, dry weight and germination rate were decreased distinctively. This decrease of seed dry matter resulted from the decrease of the cotyledon weight, and the ratio of seed coat to whole seed was increased. For the change of chemical compositions by the decrement of seed dry weight during the storage, protein, oil, and carbohydrate contents of the seed coat were decreased, and protein content of the cotyledon was markedly decreased in the seeds stored for 3 years. The ratio of seed coat to whole seed was higher in small seed than large seed cultivars. In the local varieties, protein and oil contents were 43.59% and 18.25% in the cotyledons, respectively, which were lower than those of recommended cultivars. But starch and sugar contents were 5.70% and 11.58%, respectively, which were higher than those of recommended cultivars. Sucrose, raffinose and stachyose contents were also higher in the cotyledon of the local varieties.

• Journal of Bio-Environment Control
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• v.13 no.3
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• pp.135-142
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• 2004

The objective of this study was carried out to determine which method is effective for improving seed germination in carrot and onion among osmotic or solid matrix priming. Seeds were osmotic primed with -0.5 MPa polyethylene glycol 8000 (PEG) solution and solid matrix primed with Micro Cel E (seed, Micro Cel E, and water mixed with the ratio of 4.0：2.0：8.0 by weight for carrot, and the ratio of 4.0：1.2：5.0 for onion at $20^{\circ}C$ for 4 days). Seeds were rapidly absorbed water after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in carrot and onion. Osmotic priming and solid matrix priming did not influenced on increasing of percent germination, but $T_{50}$ of treated seeds was shorter than those of untreated seeds at four temperature regimes. The effects of osmotic or solid matrix priming in reducing the $T_{50}$ was greater when the seeds were germinated at $15^{\circ}C$ than at $25^{\circ}C$ and $30^{\circ}C$ temperature. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in both carrot and onion. After priming, surface-dried seeds germinated faster than dried-back seeds in carrot and onion. Emergence speed of carrot and onion seeds was faster than untreated seeds by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of carrot and onion seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.535-541
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• 1996

A seed pelleting technique was developed for easy handling of small tobacco seeds (variety, NC82) and for direct seeding in temperary planting bed or in field. The mixture of pelleting material, binder and seeds were moulded in cylindrical holes sized 2 mm diameter and 2 mm height in a plastic plate. Bentonite and cellulose powder were good materials to make pellets with CMC as binder, and bentonite formed the hardest pelleted seeds among the materials. The number of the pelleted seeds made with the same weight of the materials was different with materials used and the number of seeds contained in a pelleted seed could be controlled by mixture ratio of materials and seeds. The seedless pellets ranged 6.9 to 16.0% at the ratio of pelleting material and seed for 2~3 seeds in a pellet. The moisture absorption rate at 100% RH and $25^{\circ}C$ was greater in the order of clay < bentonite < cellulose. Germination rates of pelleted seeds with bentonite and cellulose were similar to that of usual seed, but it was significantly lower with clay pelleted seeds.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.134-142
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• 2008

This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.44-51
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• 1995

This investigation was conducted to determine the effect of the optimum planting date and place for good seed production on growth variation, grain yields, different of variety, infection of seed by fungi, and seed germination and vigor after room storage. Early maturity Korean soybean variety, Keunolkong, and introduced vegetable soybean variety, Fukura-edamame, were planted at field of YAES. and high cool land of Sajapyong in Milyang（altitude, 850m） on May 15 （early）, June 15 （mid.）, and July 15 （late） in 1991. The emergence and vegetative period gradually increased in the early planting date. The grain yield, seed weight, pod number, healthy grain yield also increased when Keunolkong was planted on early date. The healthy seed rate, Keunolkong increased in the early planting date, whereas that of Fukura also increased in the late planting date. Infection ratio of grain to phomopsis seed decay （Phomopsis spp. ' Diaporthe phaseolorum） in Fukura steadily increased in the early planting date. Infection ratio of grain to purple seed stain （Cercospora kikuchii） generally increased in the mid and late planting date. The seed germination and seedling vigor after room storage from five to six months gradually increased in the late planting date. And seed germination of Fukura rapidly decreased in the early planting date. Therefore, the optimum planting date for good seed production in early maturity vegetable soybean was June 15 in terms of harvesting time avoid a high temprature and humidity.

• Applied Biological Chemistry
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• v.39 no.1
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• pp.25-31
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• 1996

Transgenic tobacco plants expressing calmodulin derivative($lys{\rightarrow}ile$ 115 calmodulin) and hygromycin resistance genes or hygromycin resistance gene alone(control) were generated by Agrobacterium-mediated DNA transfer. Seeds obtained from the transgenic plants($F_o$) were tested for resistance to hygromycin and the expected 3 : 1 ratio was observed. The expression of calmodulin derivative in the tobacco plants was identified by a combined method of Western blot and Chemiluminescence. The effects of surface sterilizers on the germiation of seeds from transgenic tobacco plants were tested in Murashige and Skoog agar medium. Seeds obtained from transgenic tobacoo plants expressing the calmodulin derivative showed no fungi contamination with normal germination by treating with sterilized water alone or sodium hypochlorite(2% effective chlorine). In contrast, seeds from the control transgenic tobacco plants showed severe contamination with fungi by treating with sterilized water alone and showed no contamination with normal germination by treating with sodium hypochlorite(2% chlorine). The effects of calcium concentration on the germination of seeds from transgenic tobacco plants were tested in Murashige and Skoog agar medium. Seeds obtained from transgenic tobacco plants expressing the calmodulin derivative showed better germination frequency than that of the control transgenic tobacco seeds in the medium containing 30 mM $CaCl_2$. The data raise the possibility that the expression of calmodulin derivative gene in tobacco plants could increase adaptability of the seeds to environmental stresses.

• Journal of Ginseng Research
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• v.20 no.1
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• pp.83-87
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• 1996

The effects of gibberilin ($GA_3$) on levels of endogenous indole-3-acetic acid (IAA) and zeatin in both fresh and stratified American ginseng (Panax quinquefolium) seeds were investigated. In our first experiment, the fresh seeds were stratified after soaked in 80 ppd $GA_3$ solution for 24 hours. We found that the IAA concentration in embryo increased by 50.7% and 82.1% respectively at the 120th day and the 188th day of stratification, and the zeatin concentration also increased by 3.8% and 51.6% respectively. In our second experiment, we treated the seeds after 134 days stratification with 80 ppm GA3 for 24 hours and then continued to stratify them. We found that the IAA concentration in embryo increased by 32.9% and 17.7% respectively at the 164th day and the 208th day of stratification while zeatin concentration increased by 22.7% and 30.6% respectively In our another experiment, we studied the effects of $GA_3$, abscislc acid (ABA) and GA, plus ABA on germination rate of seeds treated with these plant hormones during stratification. We found that when the stratified seeds whose ratio of embryo had reached 75% were treated with 80 ppm GA3 for 24 hours and then were allowed to be stratified for another 88 days, the weight and length of embryo (p < 0.05), and germination rate (p < 0.01) increased. In contrast, the 25 ppm ABA treated with for 24 hours was found to Inhibit the growth of embryo (p < 0.05) and reduce the germination rate (p < 0.05) . The experiment of combination treatment of $GA_3$ and ABA showed that $GA_3$ could relieve the inhibitory effects of the ABA on the development of the seeds.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.118-123
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• 2008

For effective production of interspecific hybrids of Lilium and valuable new cultivars, we used Lilium Asiatic hybrid 'Tiny Ghost' as the female parent, and Oriental group, and Longiflorum group and Martagon as the male parents. Percentage pollen germination in Oriental hybrid 'Sorbonne' was the highest with 64%. As results of using normal style pollination and cut style pollination methods, all seed sets were formed in normal style pollinated treatment, except in 'Aktiva'. Pollen germination percent of Longiflorum hybrid 'Norina' was higher than that 'Gelria'. However, all crossings using 'Norina' failed to set seeds. Corresponding fertilization ratio was different from genetic compatibility, even in the same group. From the crossing using 'Aktiva' as the male parent one bulb was obtained derived from one embryo sac. And using 'Sorbonne' as the male parent, one plantlet was obtained after four ovule cultures. Crossing using 'Gelria' as the male parent five embryos, 15 embryo sacs, and seven ovules per one ovary were obtained. Among them, 18 plantlets germinated, with the germination percent of 66.7%. Crossings using L. hansonii, Martagon group, as the male parent resulted in 40% germination from five ovules.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.128-133
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• 2008

This study was conducted to select new variants with different characteristics in plant height, leaf color, and petal color of plantain lily (Hosta plantaginea) and Japanese farfugium (Farfugium japonicum). After 10-30Gy ${\gamma}-ray$ irradiation to seeds of both species, seed germination, variant induction, growth of variants, and survival ratios were estimated. Days required for seed germination were lengthened and % seed germination decreased as the dosage of ${\gamma}-ray$ increased over 20Gy. Lethal dose ($LD_{50}$) for plantain lily and Japanese farfugium were 30Gy and 25Gy, respectively. Plantain lily and Japanese farfugium treated by more than 20Gy ${\gamma}-ray$ showed dwarf characteristics in growth, and variations in leaf and petal colors. Total 166 variants in plantain lily were induced by ${\gamma}-ray$ irradiation in a $M_1$ generation, among which 12 promising variants were selected in a $M_2$ generation. Total 57 variants of Japanese farfugium were induced by ${\gamma}-ray$ irradiation in a $M_1$ generation, among which nine promising variants were selected in a $M_2$ generation.