• Title/Summary/Keyword: germination index

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The Use of Multiple Tests in Predicting the Vigor of Soybean Seeds (대두 종자 발아세 검정방법 비교)

  • Kim, Seok-Hyeon;Copeland, Lawrence O.;Baalbaki, Riad
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.3
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    • pp.268-276
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    • 1987
  • Five soybean varieties were used to measure seed vigor according to the artificially different deterioration. A vigor index derived from the product of percent germination x hypocotyllength was used as the parameter in comparison of other tests for seed vigor. There were the varietal differences in initial vigor. The warm germination test was the best measurement for seed vigor at the advanced stages of seed deterioration. All other vigor measurements, except ATP and GADA measurements, showed highly significant correlations with the vigor index. Hypocotyl length, conductivity index and cold germination measurements for seed vigor were appropriate for predicting seed quality.

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Effect of light, ultrasonication and liquid smoke on germination of proso millet (Panicum miliaceum L.) seeds

  • Kim, Min Geun;Kim, Young Ae;Jung, Ki-Yeul;Kim, Du Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.213-213
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    • 2017
  • High quality seed of proso millet that has high germination percentage, germination speed, and uniformity demanded to increases rates of mechanization in cereal crop cultivation. In order to improve germination characteristics, proso millet seeds were treated with red light, ultrasonication and liquid smoke (LS) solution that generated from hickory wood. All treatments were performed in seed priming solution with 100mM $NH_4NO_3$ at $15^{\circ}C$ for 24hrs under aeration condition. Seeds were exposed under light intensity of 2000 lux for 15m, 30m, 60m, and 120m in priming solution. Ultrasonic treatment was performed at 60%, 80%, and 100% intensity of 21.6 KHz for 5m, 10m, and 20m in priming solution. For LS treatment seed were soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted solution with $dH_2O$ or 100mM $NH_4NO_3$ solution. The effect of seed treatment was evaluated with germination percentage (GP), mean germination time (MGT), germination index (GI), germination rate (GR), Germination uniformity (GU) and heath seed percentage (HS). Our results demonstrate that red light (15min) or ultrasonication (21.6kHz, 5min) treatment improved MGT, GI, GR, and GU comparing to untreated control. Importantly, we show that LS treatments have significant effect on the health seedling and germination characteristics. Proso millet seeds that treated with 5% LS solution for 24hrs improves HS up to 97% that similar results obtained in 100mM $NH_4NO_3$ priming for 24hrs. The combined treatment with LS solution and 100mM $NH_4NO_3$ priming were not effective in all treatments. Our results demonstrate that treating seeds with LS or 100mM $NH_4NO_3$ priming or ultrasonication improves germination characteristics. The methods described here will help advance research using this species by increasing the germination performance at which successive seed pellet process.

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Germination and Growth Performance of A Native Threatened Tree Species Quercus gomeziana A. Camus in Nursery Stage: Case of Bangladesh

  • Nandi, Rajasree;Dey, Soma;Hossain, Mohammed Kamal
    • Journal of Forest and Environmental Science
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    • v.36 no.1
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    • pp.1-6
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    • 2020
  • This study was conducted to investigate the effect of different pre-sowing treatments of seeds on germination and growth performance of native threatened tree species Quercus gomeziana A. Camus at the nursery of Chittagong University, Bangladesh. Furthermore, seedling growth attributes under different doses of fertilizer (urea) was also experimented to find the best dose of fertilizer on this tree species at the nursery stage for better field level growth. Seeds were placed to six pre-sowing treatments e.g. control (PT0), treated with sand paper rubbing (PT1), nicking (PT2), seeds immersed in cold water for 48 hours (PT3), seeds immersed in cold water for 7 days (PT4) and seeds sown at propagator house with increased temperature (PT5). It was found from the study that germination was started earlier (at 31 days) in treatments sand paper rubbing (PT1) and nicking (PT2). The highest germination percentage (93%) was in PT1 followed by 86% in seeds immersed in cold water for 7 days (PT4) and 80% in PT0 (control). Germination percentage was observed least (63%) in PT2 even though germination started earlier. For fertilizer dose experiment to seedlings at the nursery level, treatment FT1: 100 kg/ha (0.33679 g urea/pot/seedling) comparing with other treatments FT0: 0 kg/ha (Control), FT2: 200 kg/ha (0.67358 g urea/pot/seedling), FT3: 300 kg/ha (1.01037 g urea/pot/seedling) showed better performance in case of collar diameter (6.74 mm), number of leaves, shoot dry weight (19.74), total dry weight (28.16 g), total fresh weight (67.96 g), volume index (3904.82), sturdiness (127.69). Finally, it can be concluded that Quercus gomeziana seedlings revealed better performances under the treatment FT1 in growth and biomass production. Findings of this study will be helpful to take decision on organic fertilizer dose application to seedlings of Q. gomeziana for large scale plantation and conservation of this species.

Bioassessment of Nanoparticle Toxicity based on Seed Germination and Germination Index of Various Seeds (다양한 씨앗의 발아 및 발아지수에 근거한 나노입자 생물학적 독성평가)

  • Gu, Bon Woo;Lee, Min Kyeung;Shi, Yu Tao;Kong, In Chul
    • Clean Technology
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    • v.21 no.1
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    • pp.39-44
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    • 2015
  • This study investigated the effects of six metal oxide nanoparticles (NPs: CuO, NiO, TiO2, Fe2O3, Co3O4, ZnO) on seed germination and germination index (G.I) for five types of seeds: Brassica napus L., Malva verticillata L., Brassica olercea L., Brassica campestris L., Daucus carota L. NPs of CuO, ZnO, NiO show significant toxicity impacts on seed activities [CuO (6-27 mg/L), ZnO (16-86 mg/L), NiO (48-112 mg/L)], while no significant effects were observed at > 1000 mg/L of TiO2, Fe2O3, Co3O4. Tested five types of seed showed different sensitivities on seed germination and root activity, especially on NPs of CuO, ZnO, NiO. Malva verticillata L. seed was highly sensitive to toxic metal oxide NPs and showed following EC50s : CuO 5.5 mg/L, ZnO 16.4 mg/L, NiO 53.4 mg/L. Mostly following order of toxicity was observed, CuO > ZnO > NiO > Fe2O3 ≈ Co3O4 ≈ TiO2, where slightly different toxicity order was observed for carrot, showing CuO > NiO ≈ ZnO > Fe2O3 ≈ Co3O4 ≈ TiO2.

Influence of low temperature and sowing depth on emergence in dry direct seeded rice (벼 건답직파재배(乾畓直播栽培)시 저온발아성(低溫發芽性)과 파종심(播種深)에 따른 출아특성(出芽特性))

  • Kim, Hyun Ho;Lee, Joo Yeol
    • Korean Journal of Agricultural Science
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    • v.24 no.2
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    • pp.126-131
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    • 1997
  • This experiment was conducted to obtain the basic information on ability of germination at low temperature with 26 cultivars of Korea including USA cultivars and to identify the optimal sowing depth in dry-direct seeded rice. Varietal difference in germination rate and days to emergence at low temperature($13^{\circ}C$) was observed. Average germination was 81.2% and range of germination rate showed 64.3 to 94.8%. In days to germination there was varietal difference. Average days to germination was 17.4 days and those range showed 14 to 24days. Negative high correlation was observed between the ability of germination and days to germination. Considering germination and days to germination. Jakwangdo, Nagdongbyeo, Cheongmeongbyeo, Calom, Anjungbyeo, Gyehwabyeo, Daegwanbyeo, Dongjinbyeo and Seohaebyeo were selected as a adaptable cultivar. High correlation was observed among the lodging index and sowing depth, stem diameter, height of central gravity, breaking strength. Seedling emergence was increased and days to emergence was shortened at the seeding depth of 1 and 3cm. Lodging index was decreased as seeding depth was deep and so the reasonable seeding depth would be around 3cm in direct seeded rice.

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Effect of Priming Treatments on Seed Germination and Seedling Growth of Sorbus alnifolia (Priming 처리가 팥배나무의 종자 발아 및 유묘 생장에 미치는 영향)

  • Seo, Byeong-Soo;Choi, Chung-Ho;Park, Woo-Jin
    • Korean Journal of Plant Resources
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    • v.22 no.1
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    • pp.5-12
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    • 2009
  • Seed priming is a useful technique for rapid and uniform seed germination as well as early seedling establishment. This experiment was conducted to find out the optimum condition for Sorbus alnifolia seed priming with four concentrations of four reagents in germination property and seedling growth performance. The results are summarized as follows: Percent germination (PG) varied 2.67% to 24.67%, and S. alnifolia seeds had the highest PG in the treatment that were primed in 100mM $KNO_3$ solution for 2 days. Mean germination time was the shortest in 200 mM $KNO_3$ solution for 2 days. Seed priming with $KNO_3$ solutions increased germination speed (GS) and germination performance index (GPI) compared with non-primed seeds. Especially seed primed with 100 mM $KNO_3$ solution for 2 days showed the highest GS and GPI. The highest relative growth rate (RGR) and seedling vigor index (SVI) was significantly (p<0.05) different from the control and other treatments, respectively. RGR of height (0.0071) and root collar diameter (0.0141) of seedling from primed seeds were the highest in 400 mM NaCl solution for 2 days. The highest SVI (5.43) was observed in the seedlings from seeds primed in 100 mM $KNO_3$ solution for 2 days. Consequently, the optimum reagent and concentration were $KNO_3$ and 100 mM for the effective germination and seedling growth in S. alnifolia

Effect of Seawater Concentration on Seed Germination and Seedling Growth of Artemisia fukudo (해수농도가 큰비쑥의 종자발아 및 유식물 생장에 미치는 영향)

  • Ko, Myoung-Suk;Bae, Kee-Hwa;Lee, Mi-Hyun;Kim, Nam-Young;Lee, Yeon-Kyung;Han, Myoung-Soo;Song, Jae-Mo;Song, Gwanpill
    • Journal of Forest and Environmental Science
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    • v.30 no.1
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    • pp.120-125
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    • 2014
  • Halophytes are plants of saline habitats that grow under conditions that may vary in extremes of temperatures (freezing to very hot), water availability (drought to water logging) and salinity (mild to almost saturation). Halophytes may also face sudden micro-environmental variations within their habitats. In this study, we examine some of the factors that determine the ability of seeds of Artemisia fukudo to germinate when conditions are optimal for seedling growth and survival. Germination percentage was the highest at 77.5% when treated in 0% seawater and seedling growth was best in 0% seawater plug cell tray. Physiological quality (chlorophyll contents) and mean germination time were best in the condition in which seeds were treated with 0% seawater (control). Germination performance index in the control group showed the highest value to 3.8. Seedling growth, seedling vigor index in the length of seedling growth andthe relative growth rate were the highest values of 0.76 cm, 1.43, 0.0099, respectively.

The effect of hydropriming and explants on shoot initiation and tetraploids in small watermelons

  • Phat, Phanna;Noh, Jae Jong;Ju, Ho-Jong;Lee, Gi-An;Ma, Kyung-Ho
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.176-176
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    • 2017
  • Hydropriming had positive effects on the time for germination to reach 50%, the germination index, the time to final germination percentage, and the number of uniform seedlings with enlarged cotyledons in in vitro germination of small watermelon. In addition, the highest shoot initiation was obtained from hydroprimed cotyledonary nodes ($95{\pm}6%$), followed by non-primed cotyledonary nodes ($78{\pm}6%$), hydroprimed cotyledons ($72{\pm}4%$), and non-primed cotyledons ($48{\pm}4%$). Meanwhile, no shoots were initiated from hypocotyls. The total number of shoots that initiated from cotyledonary nodes and cotyledon explants was insignificant, indicating that both cotyledons and cotyledonary node were good sources for the in vitro culture. Choosing explant sources that favor tetraploidy should be the key for producing higher polyploidy plants; a total of 10.5% of tetraploid regenerants were entirely identified from cotyledon explants. Cotyledons with highly differentiated cells might show higher variations than cotyledonary nodes with more preexisting meristematic cells. Cells of cotyledon tissue might undergo changes in ploidy level during differentiation of the culture, or it might be that some of the variations were already present in the tissues of the donor plant. Morphological changes in fruit length of tetraploid regenerants are genotype-dependent.

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Seed Germination of Surface Soil for Restoration of Disturbance Place - Bare Land and abandoned Field, Yesan-gun, Korea - (교란지 복구를 위한 표토의 매토종자 발아특성 - 예산군의 나지와 묵밭 토양-)

  • Kang, Hee-Kyoung;Cho, Nam-Kyoung;Song, Hong-Seon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.20 no.6
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    • pp.79-92
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    • 2017
  • To offer the basic data of vegetation restoration by buried seed of soil, this research was conducted at bare land and abandoned field in Yesan-gun. Germination plants of buried seed were consisted of 40 taxa (37 species, 3 varieties) in bare land, and 41 taxa (37 species, 4 varieties) in abandoned field. Classification of germination plants by family was the most in Gramineae, and emergent frequency of plots was the highest of Digitaria ciliaris. The soil depth of the most plants appearance was 2~5cm in bare land and 5~10cm in abandoned field, and the soil depth of the most population appearance was 0~2cm both in bare land and in abandoned field. Population number of buried seed germination was decreased according to soil depth. Crepidiastrum sonchifolium was a plant that population number of buried seed germination is the most. Similarity index was 0.33 in aerial part plants and buried seed plants, and 0.55 in bare land and abandoned field.

Optimum Storage Temperature for Spring Sowing of Panax ginseng Seeds (봄파종을 위한 인삼 종자 저장 적정 온도 연구)

  • Suh, Su Jeoung;Jang, In Bae;Jang, In Bok;Moon, Ji Won;Yu, Jin
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.6
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    • pp.390-396
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    • 2019
  • Background: Usually ginseng seeds are sown during autumn and spring. Sowing in spring often results in poorer seedling establishment than in autumn. One of the reasons for poor germination could be cold-treatment condition for breakage of physiological dormancy during winter. Here we tested the effects of storage temperature used during cold treatment on germination. Methods and Results: Germination properties were observed after dehiscent seeds were stored as wet and dry at 2℃, -2℃, -3.5℃ and alternating temperature (AT). Seed dryness and storage temperature affected germination properties (p < 0.01). Wet and AT condition germinated highest, and wet and -3.5℃ condition germinated lowest, which was 91.2% and 1.4% respectively. Mean germination time (MGT) of the wet and AT condition was faster than other treatments at 2.4 days, and the dry and -2℃ condition was the longest. Germination performance index (GPI) was highest for wet and AT condition (37.7%) and the lowest for wet and -3.5℃ condition (0.5%). The growth of above-ground and below-ground were the best for wet and 2℃ condition, and wet seeds showed better growth than dry seeds (p < 0.01). Conclusions: For cold treatment, ginseng seeds may not be stored below -2℃ for successful germination during spring sowing.