• 한국잡초학회지
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• 제10권3호
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• pp.221-226
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• 1990

상치의 생물검정(生物檢定)을 통(通)해 독활로부터 생장억제물질(生長抑制物質)을 분해(分解) 동정(同定)한 결과(結果) Tlc상에서 $R_f$ 0.51로 나타났으며 이 물질(物質)은 상치생체중(生體重)을 70% 억제(抑制)하였으며 3% $FeCl_3$(0.5N HCL) 시약(試藥)에 검푸른 반응(反應)을 보여 phenol성(性) 물질(物質)로 확인(確認)되었고 324nm에서 가장 높은 UV 흡수대가 나타났고 IR spectrum에서 $3,600cm^{-1}$에서 OH기, $1,700cm^{-1}$에서 C=0, $1,600cm^{-1}$에서 C=C, $1,200cm^{-1}$에서 C-0결합(結合)이 확인(確認)되었다. HPLC에 의한 동정(同定) 결과(結果) retention time 25분(分)대의 ferulic acid($C_{10}H_{10}O_4$)와 일치(一致)하여 독활의 주(主) 억제물질(抑制物質)이 ferulic acid임을 확인(確認)하였다.

• 한국작물학회지
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• 제39권5호
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• pp.420-425
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• 1994

올콩의 수확시기에 따른 품질변화를 밝히고자, 대립종인 Yuhsuzumi와 소립종인 담양재내를 공시하여 수확시기별 종실의 수분함량, 입색, 발아율, 지방산 조성 등을 조사하여 다음과 같은 결과를 얻었다. 1. 협과 종실의 수분함량은 R7단계 전후까지는 협의 수분함량이 뚜렷이 높았으나, R8단계 이후에는 종실의 수분함량이 협보다 다소 높은 경향을 보였으며, 종실의 수분함량이 14%(종실 수분함량으로 본 수확적기) 내외가 되는 시점은 Yuhsuzumi에서는 생리적 성숙기(R7단계)로부터 24일, 담양재내는 8일이 소요되었다. 2. R8단계(고유 성숙협색기)에서 종실의 수분함량은 대립종인 Yuhsuzumi는 25.3%, 소립종인 담양재내는 14.5%를 나타내어 R8단계에 수확시 Yuhsuzumi는 철저한 건조과정이 필요하였다. 3. 수확기 지연에 의하여 Yuhsuzumi는 청색의 종실이 황변하고 입질이 저하되어 발아력이 낮아졌으나, 담양재내는 입질과 발아력의 차이가 없었다. 4. 수확기 지연에 의하여 Yuhsuzumi는 지방산 함량이 낮아졌고, 지방산 조성에 있어서는 Lino-lenic 산은 낮아지고 Oleic산은 증가되었다.

• 한국잡초학회지
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• 제15권4호
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• pp.247-253
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• 1995

The herbicide butachlor [N-(butoxymethyl)-2-chloro-N-(2,6-di-methylphenyl) acetamide] is widely used by farmers as a tool for weed management of transplanted rice(Oryza sativa L.) in Taiwan. The herbicide did not stop germination of rice and weed seeds, but strongly inhibited the subsequent growth of young shoots and roots. The inhibition was also strong on established seedlings. However, they could recover to normal growth after the herbicide effect disappeared. Butachlor greatly decreased the endogenous indole-3-acetic acid (IAA) but increased the endogenous abscisic acid (ABA) contents of rice seedlings. Addition of lAA into growth medium (Hoagland's solution) partly relieved growth inhibition. Pretreatment of both gibberellic acid ($GA_3$) and IAA 24 hours before butachlor treatment almost completely alleviated the butachlor-interfere with GA and/or IAA metabolism or their action resulting in the growth inhibition of rice. Butachlor was readily absorbed by rice roots. During 24 hours of uptake experiment, 32% of the applied herbicide was absorbed. Pretreatment of the herbicide for 2 days did ncx affect the absorption. Of the absorbed herbicide, 80% remained in roots, only 20% transported into shoots, and more than 50% was metabolized to water soluble substances. Thin-layer chromatographic (TLC) analysis indicated that the Rf value of the most abundant metabolite was butachlor-glutathione conjugate. Rice, barnyardgrass (Echinochloa crus-galli (L.) Beauv.), and monochoria (Monochoria vaginalis Presl) seedlings contained relatively high level of non-protein thiols, while the glutathione S-transferase (GST) activity was found highest in rice, barnyardgrass the next, monochoria the lowest. The difference in GST activity among these species might be related to their sensitivity to butachlor.

• Applied Biological Chemistry
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• 제24권1호
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• pp.67-73
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• 1981

보리와 발아보리의 생체내 영양효율을 알기위해 동물실험을 행하였다. 젖뗀 albino rat를 사용하여 단백질원으로 보리 및 발아보리만을 사용한 군, 단백질(5%의 casein)을 첨가해준, 그리고 쌀(쌀:보리=8:2) 및 단백질(5% casein)을 혼합 첨가시킨군으로 나누어 4주간 사육하여 다음과 같은 결과를 얻었다. 1. 식이 및 열량섭취량, 식이의 효율 및 체중은 보리 및 발아보리에 단백질을 첨가시킨군들에서 높았으며 쌀과 단백질을 첨가시킨군들에서는 현저히 높아 통계적으로 유의성을 보였다. 그러나 보리군들과 발아 보리군들간의 차이는 볼 수 없었다. 2. 단백질의 효율은 단일 곡류군들과 단백질 첨가군들에 비해 쌀과 단백질 혼합첨가군들이 높음을 보여 흔합된 곡류단백질이 체내효율을 높이는 것으로 나타났다. 3. 식이 및 단백질의 소화흡수율을 보면, 단일곡류군들에서는 표준군에 비해 보리군 및 발아보리군들이 낮음을 보였다. 그러나 여기에 단백질 또는 쌀과 단백질을 혼합 첨가하였을 경우 보리군들에 비해 발아보리군들의 단백질 소화흡수율이 현저히 증가하였다. 따라서 발아보리의 영양효과는 발아보리와 함께 동물성단백질을 적당히 섭취할 경우 단백질의 소화흡수율을 크게 증가 시키는데 있다고 할 수 있다.

• 식품보건융합연구
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• 제6권1호
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• pp.9-15
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• 2020

Salinity with sodic condition disturbs germination, retards emergence, and slow down seedling development of Lemon Grass (Cymbopogon citratus).Lemongrass is a perennial grass plant widely distributed worldwide and most especially in tropical and subtropical countries. This research experiment was designed to evaluate the influences of (4 dSm^-1+ 13.5 (mmol L^-1)^-1/2, 5 dSm^-1+ 25 (mmol L^-1)^1/2, 5 dSm^-1+ 30 (mmol L^-1)^1/2, 10 dSm^-1+ 25 (mmol L^-1)^1/2 and 10 dSm^-1+ 30 (mmol L^-1)^1/2) on biomass produce of lemon grass against salt tolerance. The uppermost biomass yield (45.53 gpot^-1) was produced by 4 dSm^-1+ 13.5 (mmol L^-1)^1/2 treatment. The increase in the intensity of salts reduced the growth of lemon grass. Lower biomass yield (79.33 gpot^-1) was gained at 10 dSm^-1+ 30 (mmol L^-1)^1/2. 5 dSm^-1+ 25 (mmol L^-1)^1/2 treatment performed enhanced outcome i.e. the least reduction % over control (5.87). Salinity- sodicity showed serious effect on the growth reduction from 5.87% to33.60%. This reduction gap was affected by the negative effect of salinity and sodicity on Linseed growth. Salinity- sodicity showed severe impact on the growth reduction from 5.87% to33.60%. Based on the findings, lemon Grass (Cymbopogon citratus).was capable to grow up the maximum at 4 dSm^-1+ 13.5 (mmol L^-1)^1/2 treatment.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.85-89
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• 1999

Somatic embryogendesis is one of good examples of the basic research for plant embryo development as well as an important technique for plant biotechnology. This paper describes the direct somatic embryogenesis from zygotic embryos of Panax ginseng is reversely related to normal axis growth of zygotic embryos by the experiment of various chemical treatments. Under the normal growth condition, the apical tips of embryo axis produced an agar-diffusible substance, which suppressed somatic embryo development from cotyledons. Although the cells of zygotic embryos were released from the restraint of embryo axis, various factors were still involved for somatic embryo development. Electron microscopic observation revealed that the ultrastructure of cells of cotyledon epidermis markedly changed before initiation of embryonic cell division, probably indicating reprogramming events into the cells embryogenically determined state. Polar accumulation of endogenous auxin or cell-cell isolation by plasmolysis pre-treatment is the strong inducer for the somatic embryo development. The cells for the process of somatic embryogenesis might be determined by the physiological conditions fo explants and medium compositions. Direct somatic embryos from cotyledons fo ginseng were originated eithrer from single or multiple cells. The different cellular origin of somatic embryos was originated either from single or multiple cell. The different cellular origin of somatic embryos was depended on various developmental stages of cotyledons. Immature meristematic cotyledons produced multiple cell-derived somatic embryos, which developed into multiple embryos. While fully mature cotyledons produced single cell-derived single embryos with independent state. Plasmolysis pretreatment of cotyledons strongly enhanced single cell-derived somatic embryogenesis. Single embryos were converted into normal plantlets with shoot and roots, while multiple embryos were converted into only multiple shoots. GA3 or a chilling treatment was prerequisite for germination and plant conversion. Low concentration of ammonium ion in medium was necessary for balanced growth of root and shoot of plantlets. Therefore, using above procedures, successful plant regeneration of ginseng was accomplished through direct single embryogenesis, which makes it possible to produce genetically transformed ginseng efficently.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제1권1호
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• pp.22-30
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• 2020

Many areas of vegetation in the British uplands have reduced species diversity as a result of sheep overgrazing. It has been suggested that abandonment or re-wilding strategies might be used to reverse this. A likely first step would be the removal or reduction of grazing livestock from upland areas, with a presumption that this would lead to a recovery in species richness. However, we do not know if this would work, or the timescales involved. One of the important areas where more knowledge is needed is information on the size and composition of soil seedbanks as regeneration from zseed is a likely pathway of recovery. Here, we compared seedbanks in both grazed and ungrazed plots in five experiments at Moor House NNR in the northern Pennines; these sheep grazing exclusion experiments were started 52 and 63/64 years ago. Soil samples (n=10) were collected from both grazed and ungrazed plots in each experiment, and seed emergence counted in glasshouse trials. We detected only seeds of common species and very few dicotyledonous species. This suggests that the soil seedbank is unlikely to be a reliable source of the less common species for ecological restoration in these upland communities, suggesting an extinction debt. Therefore, seed addition and the creation of suitable safe-sites for germination may be needed in conjunction with grazing controls to allow the establishment of plants that will increase the species richness of the vegetation. However, this interventionist restoration approach remains to be tested.

• 한국잡초학회지
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• 제17권4호
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• pp.421-430
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• 1997

채취시기(採取時期)를 달리한 초록과 노란은행잎으로부터의 수용추출물(水溶抽出物)에 대한 벼, 피, 바랭이 및 상추의 제초활성검정(除草活性檢定)과 지방산(脂肪酸) 성분(成分)과 페놀성 물질(物質)을 분리(分離) 동정(同定)하여 얻어진 결과(結果)는 다음과 같다. 1. 은행잎 수용(水溶) 추출물(抽出物)은 벼, 피, 바랭이, 상추의 발아(發芽)와 생육(生育)을 크게 억제(抑制)시켰으며, 특히 20% 수용추출액(水溶抽出液)인 경우는 억제효과(抑制效果)가 뛰어났다. 초록과 노란은행잎 수요 추출물간의 생리활성정도는 별 차이가 없었다. 2. 은행잎의 지방산(脂肪酸)을 검정(檢定)한 결과(結果), 지방산(脂肪酸)이 검정(檢定)되었으며, 그 중에서도 Linolenic acid, Palmitic acid가 많이 포함(包含)되어 있었다. 또한 계절에 따라 은행잎에 함유(含有)된 지방산(脂肪酸)의 함량(含量)이 다소 변화(變化)함을 알 수 있었다. 3. GLC로 phenolic acids를 동정(同定)한 결과(結果), 모든 fraction에서 Salicylic acid의 높은 비율(比率)을 관찰(觀察)할 수 있었으며, 또한 p-coumaric acid, phloroglucinol 등도 검정(檢定)되었다.

• 한국자원식물학회지
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• 제33권6호
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• pp.682-688
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• 2020

Hemp (Cannabis sativa L.) is a dioecious plant, although monoecious plants are bred in some cultivars for fiber or seed production. Recently, hemp has received attention for medicinal use with some cannabinoids, including cannabidiol. Self-fertilization for breeding inbred lines is difficult because of dioeciousness and anemophily in hemp. This experiment was conducted to develop a self-fertilization method by forming female flowers and seeds from male plants of dioecious hemp. To induce the formation of female flowers on male plants, 500 mg L^-1 of ethephon was sprayed on plants at soon, seven and fourteen days after primordia formation. The plant ratio of female flowers formation and the number of harvested seeds were increased by ethephon treatment. Female flowers of male plants have 5 stigmas in contrast to the dual stigma of female 1plants. Male plant seeds were lighter and smaller than those from female plants. Although the germination rate was lower than that of normal seeds from female plants, the seeds from male plants germinated to grow seedlings. Thus, we suggest that hemp plants should be treated with ethephon at soon after primordia formation to induce the formation of more female flowers on the male plants.

• 한국작물학회지
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• 제51권1호
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• pp.59-65
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• 2006

The impermeable seed coat is valuable trait in soybean because impermeable seed retain viability for longer period than permeable seed under adverse conditions such as delayed harvest or prolonged storage. Soybean seeds of various size showing different seed hardness were examined for their water absorption and seed viability under adverse storage conditions. Of one hundred thirty nine genotypes, eight types of seeds having different seed hardness and seed size were used as material. Soybean genotypes showing high hard seed rate, GSI13125 (89%), GSI10715 (54%), and GSI10284 (42%), were slow in water absorption and low in the electroconductivity of seed leachate in distilled water. Germination of GSI10284 and GSI13125 that have higher hard seed rate was less affected by CSVT and artificial aging treatment indicating higher seed storability. The higher storing ability of both collections was confirmed by electroconductivity test for leachate. GSI10122 showed low seedling emergence when the seeds were artificially aged. This genotype was considered as to having a poor storing ability based on difference of electroconductivity before and after artificial aging. Among tests conducted in the experiment, CSVT could be used for determining storage life in legumes. In conclusion, water absorption property of seed was strongly related to the hardness that is directly related to the seed viability and storing ability in soybean seed.