• Title/Summary/Keyword: germination experiment

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Effects of Seed Size and Several Factors on Ultra-drying and Germination of Ultra-dried Seeds in Soybean

  • Lee, Yeong-Ho;Chang, Ching-huan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.305-309
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    • 2000
  • Ultra-drying [<5.0% seed moisture content (SMC)] storage technique is a cost-effective storage method for oily seeds. To decide proper ultra-drying condition for soybean seeds, drying rate was compared three silica gel to seed ratios, two seed sizes with varietal difference, two kinds of container, and three seed amounts per container under :t 23$\pm$1$^{\circ}C$. When the relative humidity (RH) was reduced at the rate of less than 0.1 % a day, silica gel was replaced with dry one by 47 days. Higher silica gel to seed ratios (3:1 and 2:1) dried faster than lower ratio (1:1) until 28 days, but not after 43 days of drying. Also, large seeded variety was dried faster than small seeded variety. Kinds of container and seed amounts per container didn't show differences in drying of soybean seeds. After completion of ultra-drying, percentage germination by standard germination test (SGT) was not different among silica gel to seed ratios, kinds of container, and seed amounts per container, except among seed sizes (varieties). Before SGT, soybean seeds were premoistened using saturated ${CaCl}_2$ for 48 hours and ${NH}_4$Cl for 24 hours in desiccators. To compare germinability between ordinary-dried seeds and ultra-dried seeds, the seeds of seven soybean varieties, which were varying in size from 8.1 to 34.9 g per 100 seeds, were dried using same amount of silica gel under 23$\pm$1$^{\circ}C$. After completion of 76 days of drying, SMCs were reduced to 3.13-3.45% from 7.86-8.82%. SMC after completion of drying was not correlated with 100-seed weight (r=0.556). Before germination tests, soybean seeds were premoistened using saturated salt solutions. Percentage germination was higher with ultra-dried seeds than ordinary-dried seeds in SGT and higher with ordinary-dried seeds than ultra-dried seeds in AAT at the beginning of storage and after 6 months storage, but general trend of percentage germination was not observed among varieties classified by 100-seed weight. From these results, we concluded that further studies are needed to improve ultra-drying storage method for soybean seeds.

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The Allelopathic Effect of Alfalfa residues on Crops and Weeds (잡초와 작물에 대한 알팔파 잔유물의 Allelopathy 효과)

  • Yu, C.Y.;Jeon, I.S.;Chung, I.M.;Hur, J.H.;Kim, E.H.
    • Korean Journal of Weed Science
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    • v.15 no.2
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    • pp.131-140
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    • 1995
  • This experiment was conducted to test the effect on germination and seedling growth of major industrial crops and weed control potential using alfalfa plant. When dried alfalfa residues were mixed into vermiculite, germination, length of shoot and root of crops, such as Perilla frutescens, Sorghum nevosum, Platycodon grandiflorum and weeds, Digitaria saguinalis, Setaria viridis, Siegesbechia pubescens, Ammaranthus lividus, and Solanum nigrum, were significantly inhibited as the dried residue concentration increased. More than 10% concentration of the dired residue caused 80% germination and growth inhibition. The fresh alfalfa exudation also inhibited the germination and seedling growth of crop, barley, rye, alfalfa, and sesame, and weeds, Echinochloa crus-galli, Siegesbechia viridis, and Portulaca oleracea. The degree of inhibition showed the different response according to the fresh exudation concentration, types of crops and weeds. Generally, as the exudation concentration increased, the germination and seedling growth of crops and weeds inhibited. The exudation of dried residue also exhibited the strong inhibition effect on germination and seedling growth of crops, alfalfa, Platycodon grandiflorum, barley, sesame, rye and weeds, D. sanguinalis, S. pubescens, S. viridis, P. oleracea, E. crus-galli, At the 10% concentrations, S. pubescens, and P. oleracea were not germinated and showed only 15% germination in the S. viridis. From this study, would conclude that alfalfa plant contained water soluble phytotoxic substances which were inhibitory to weeds and crops. This results suggest that alfalfa had some possibility to control some weed species using toxic compounds like natural herbicide.

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Effects of Plant Growth Regulator Treatments During the Grain Filling Period on Seed and Viviparous Germination in Rice (벼 등숙기의 생장조절제처리가 종실 및 수발아에 미치는 영향)

  • Sa, Jong-Goo;Kim, Kee-Sik;Han, Se-Ki;Huh, Beom-Lyang;Kim, Kwang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.262-269
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    • 1988
  • Experiments were conducted to find out the method of reducing viviparous germination of rice seeds using growth regulators during grain filling period. Among the recommended varieties, early Japonica type showed higher rate of viviparous germination, while late Japonica type and Tongil type varieties did not show viviparous germination in field condition. Grain showed high germination ability from 30 days of grain filling period in Daeseongbyeo of Japonica type and 35 days in Taebackbyeo of Tongil type. Growth regulators, NAA, CMH and Reglone were effective for reducing viviparous germination to the two rice varieties and three treatment stages in this experiment. NAA and CMH were most effective at 100 ppm and Reglone was at 0.4-0.6% concentration. Germination rates of rice seed were not different from non-treatment when NAA CMH were treated, while Reglone lowered germination rate. NAA and CMH had no effects on grain filling rate for both varieties, which showed high grain filling rate as the non-treatment, but Reglone results low grain filling rate. Grain color was not changes when NAA was applied, while CMH and Reglone made a lot of grain to dark brown as the concentration became higher. When grain were hulled, NAA had no difference with the non-treatment whereas CMH and Reglone had lower rate of perfect brown rice when they were applied at 20-30 days after heading.

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Germination and Seedling Growth Characteristics of Seeds with Different Bolting Years in Angelica gjgas NAKAI. (참당귀(當歸) 꽃대발생(發生) 년차(年次)에 따른 종자(種子)의 발아(發芽) 및 묘생육(苗生育) 특성(特性))

  • Yu, Hong-Seob;Lee, Seung-Tack;Chang, Yeong-Hee;Kim, Kwan-Su;Kim, Young-Guk
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.3
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    • pp.193-198
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    • 1996
  • To establish appropriate seed production system in Angelica gigas. Germination of seeds with different bolting years and the growth characters of plants originated from these seeds were in­vestigated. Seed weight from 1st year bolting plant was highest. In germinability according to seeds with different bolting years, seeds from 1st year bolting plant were higher than seeds from 2nd, 3rd years bolt­ing one. In growth characters of plants originated from seeds with different bolting years, as the bolting years became late, root growth increased and top growth decreased, therefore T/R ratio decreased.

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Effects of Temperature, Seed Water Content and Osmoconditioning on Germination and Seedling Elongation of Soybeans (온도.종자수분함량 및 삼투처리가 콩의 발아 및 묘신장에 미치는 영향)

  • Rak Chun, Seong;Many C., Minor;Eun Hi, Hong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.4
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    • pp.447-453
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    • 1986
  • Germination and seedling elongation of soybeans [cult. ‘Williams’] were measured at two temperatures (15 and 3$0^{\circ}C$), three seed water contents (30, 50, and 70%), and with and without polyethylene glycol-8000 (PEG) treatments. A split-plot design was used with four replications. Observations were made from two hours to eight days for each treatment combination. Seeds were soaked with 30% PEG solution with 0.2% thiram at 15 C for six days, rinsed with deionized water and dried at 25.5$^{\circ}C$ for three days. Ten treated or untreated seeds were placed on Whatman No. I (9 cm) filter paper in plastic petridishes to which sufficient deionized water was added to adjust seed moisture content to the desired level. The dishes were then sealed with parafilm and placed in a continuous programmed temperature chamber under dark condition. Seedling growth did not occur at either temperature when moisture content was 50 percent or less. Osmo-conditioning with PEG showed positive effects on seedling moisture uptake and seedling growth at 15$^{\circ}C$ but little effect at 3$0^{\circ}C$. Seedling moisture content increased rapidly early in the germination period and reached 60% at 15 C for 56 hours and at 3$0^{\circ}C$ for 28 hours. Seedling growth started when seed moisture reached a critical point of 60% at 15$^{\circ}C$, however, growth started after 20 hours of germination at 3$0^{\circ}C$. Seedlings of soybeans elongated more than four times faster at 3$0^{\circ}C$ than at 15$^{\circ}C$. Water uptake during germination was characterized by two phases in this experiment.

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Physio-ecological Characteristics and Control of Alopecurus aequalis Sobol. var. amurensis (Kom.) Ohwi. - 1. Gernmination and Emergence Under Several Environmental Conditions (뚝새풀(Alopecurus aequalis Sobol. var. amurensis (Kom.) Ohwi.)의 생리생태적(生理生態的) 특성(特性) 및 방제(防除) - 1. 발아(發芽) 및 출아(出芽) 특성(特性))

  • Im, I.B.;Lee, S.Y.;Huh, S.M.
    • Korean Journal of Weed Science
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    • v.14 no.4
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    • pp.239-244
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    • 1994
  • As a fundamental study to control Alopeuclus aequalis Sobol. var. amurensis(Kom.) Ohwi., on dominant weed in barley and dry seeded rice cultivation on paddy fields, several factors which would have closer relations to its germination and emergence, were examined. The results are as follows. The dormancy of seeds were broken at dry-heat treatment of $30^{\circ}C$. The germination rate of the seeds was high in order of 15>10>20> $5^{\circ}C$ and was very low at more than $25^{\circ}C$. The emergence of A. aequalis was influenced little for the light, but had a tendency to be good on the dark condition. The seeds dried on room temperature germinated few but them treated on $30^{\circ}C$ for 24 hours were germinated over 80%. The A. aequalis occured few in the saline soil of salt concentration of more than 0.25% and the germination rate of seeds was very low on the over 0.5% solution of NaCl. On the solution of pH 6.0~12.0, the germination was not effected for pH but was very few on pH 2.0. At the paddy-lowland which have cultivated the soybean for three years A. aequalis emerged a few.

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Effects of Seed Disinfectant and Soaking Time on Germination and Disease Occurrence of Adlay, Coix lacryma-jobi L. var. ma-yuen Stapf (율무 종자소독과 침종시간이 발아율 및 병발생에 미치는 영향)

  • Chang, Seog-Won;Jeon, Dae-Hoon;Kim, Hee-Dong;Yi, Eun-Sup;Park, Ki-Jun
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.3
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    • pp.259-265
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    • 2000
  • This study was carried out to investigate the effect of soaking time of seed disinfectant, Fludioxonil on the germination and disease occurrence of adlay (Coix lacryma-jobi L. var. ma-yuen Stapf). The germination rate was higher in the seed disinfectant treatment for 3 days followed by seed soaking for 3 days than that of other treatments under controlled condition of low temperature. The value of two experiments were 90.8%, 96.7%, repectively. And, the time of seed soaking was longer, the more seed rot was proportionly decreased. In the pot and field experiment, germination rates were somewhat low compared to that of petri dish experiment as 88.3%, 84.3%, and occurence of seedling blight showed similar results that of petri dish experiments. There was not significantly different in the yield components among treatments in the field. However, as compared with untreated plot (194kg/10a), unhulled grain yield was increased about 26% on that treatment. Based on these results, it has been thought that improvement of the germonation rate and decrease of disease occurrence of adlay were accompanied with the seed disinfectant during 3 days followed by seed soaking during 3 days before sowing.

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Varietal Differences and Drying Storage Effects for Some Treatment Conditions of EMS and MMS on Rice Seeds (수도종자에 EMS와 MMS의 처리조건에 따른 건조저장효과와 품종간차이)

  • Young-Sang Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.55-62
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    • 1972
  • In order to study the biolgiocal effects of dry back following EMS (Ethyl methane sulfonate) treatment on seeds of 'Dunghan Shali' that belongs to indica-type rice with various presoaking(24, 36, and 40 hrs respectivily) and treatment time(l, 2 and 3 hrs respectivily), this experiment was done. Seedling heights of wet seeds presoaked for 36 hrs was only greatly decreased with EMS treatment compared with other periods. In germination rate of wet seeds, all of these damage was not changed. For the drying back seeds, otherwise, seedling height and germination rate were greatly reduced depend upon the time of treatment and presoaking as well as storage effects after drying back. Reduction patterns, both seedling height and germination rate, between 1 week and 8 weeks after dry back were quite similar. The other experiment was carried out in order to study for the influences on the seedling growth as biological damage with EMS and MMS(Methyl methane sulfonate) treatments among different varieties. The indica-type rice varieties; Taichung Native No.1, Dunghan Shali and IR-8, were used as the experimental materials. From this trial some results were obtained as follows; 1. Both seedling growth and germination rate on all varieties, used, MMS, treatment showed more toxic effects than those of EMS treatment. 2. Seeding growth injury of rice seeds dried back was increased gradually, and then was approached the maximum at 6-8 days after storing. In IR-s variety, otherwise, growth damage was appeared a little. Germination rate of these were quite smilar, even though chemical used is different and storage period was prolonged. 3. It was showed clearly that varietal differences of chemical mutagene treatments were recognized.

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