• 생명과학회지
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• 제10권6호
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• pp.598-604
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• 2000

This study was conducted to investigate the effect of simulated acid rain (SAR) on germination, growth, acid buffering capacity and nutrient leaching in Impatiens balsamina L. and Tagetes patula L.. In both species, germination was not inhibited at pH 4.0, but the germination rate decreased at the lower pH of 3.0, showing higher rate in Inpatiens balsamina L. than Tagetes patula L.. As the pH decreases, the growth of radicle was markedly decreased than that of hypocotyl in both species. The plant height, root length, leaf area, total dry weight, relative growth rate and net assimilation rate were inhibited by SAR. The acid buffering capacity in the leaves were increased at pH 4.0, on the other hand, it was shown a tendency to decrease at pH 2.0 in both species. As the pH levels decreased from 5.6 to 2.0, the nutrient leaching from leaves was significantly increased in both species. Based on the results, there are a great difference in the responses to SAR between the two species. In general, Tagetes patula L. represented a higher tolerance to SAR than Impatiens balsamina L.. These results suggested that interspecific variation in the acid buffering capacity and nutrient leaching from leaves may be responsible for the interspecific susceptibility to SAR.

• Forest Science and Technology
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• 제14권4호
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• pp.160-168
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• 2018

The seeds of Elaeocarpus serratus, a tropical underutilized fruit tree are characterized by hard seed coat and consequent poor water uptake and low germination. To improve the regeneration through seeds, various parameters such as viability of seeds, water uptake, and effect of seed mass on germination and pretreatments were performed using a completely randomized design (CRD). Tetrazolium (TZ) test was conducted using fresh, mature seeds revealed $50{\pm}2.56%$ mean viability. Seeds of different weight classes showed similar pattern of water uptake and the saturation level was achieved at 60 hrs of soaking. Seeds belong to weight class 2.6-3.5g were germinated ($12.5{\pm}1.26%$) with $175{\pm}1.75days$ (d) of mean time taken for germination (MTG). Germination capacity of seeds varied significantly among different populations and Varkala population gave $12.5{\pm}1.1%$ germination with $174.6{\pm}2.5d$ MTG. Among various seed treatments, mechanical scarification was superior in germination and significant reduction in MTG ($p{\leq}0.05$). The mechanical scarification by complete removal of seed coat resulted in $49.2{\pm}1.52%$ germination within a short period of time ($9.52{\pm}0.89d$ MTG). However, the complete removal of seed coat without damaging to embryo is a difficult task. An alternate treatment (Mechanical scarification II) by making cracks on nut faces vertically followed by soaking in distilled water for 24 hrs gave $48.4{\pm}1.73%$ germination with significantly reduced MTG ($12.14{\pm}0.56d$) over unsoaked, untreated control ($6.5{\pm}1.84%$ germination and $197.18{\pm}1.79d$ MTG; $p{\leq}0.05$). This treatment (Mechanical scarification II) is therefore recommended for E. serratus seeds as it can adopt easily and can achieve 7 fold increases in germination over control. The recorded germination through mechanical scarification is in tune with realized viability percentage of the seeds.

• 한국자원식물학회지
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• 제21권3호
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• pp.171-177
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• 2008

Variations in antioxidant capacity of leaves and characteristics of seed germination among five Hibiscus hamabo populations in Korea were evaluated. While the leaves of H. hamabo from Shincheon contained highest Na concentration, those from Hado and Ohzo does lowest. MDA content and SOD activity didn't show significant difference among populations, but total protein and anthocyanin content were significantly different among five populations. The filled seed rates of five populations were ranged from 92.0 to 98.7%, and the moisture contents of seeds from Shinyang and Soando were lower than that of R. syriacus (5.18%). The fresh weights of H. hamabo from Hado and Ohzo were the highest and those of seeds from Shinyang and Soando were the lowest. Significant variations among H. hamabo populations were detected for all seed germination characters: percentage of germination, average germination time and germination rate and uniformity. SOD activities of seeds from Onpyoung and Soando were the highest of five populations, and total protein concentrations of seeds in Ohzo and Shinyang populations were higher than those of other populations. H. hamabo is considered exposed to salt stress and all characteristics of seed germinations were inferior to other species.

• 한국작물학회지
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• 제43권3호
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• pp.194-198
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• 1998

An experiment was carried out to find out the priming effects of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) on. the seedling establishment and early emergence under excess soil moisture conditions. Seeds were primed by soaking in -0.6 MPa polyethylene glycol (PEG) solution at $25^{\circ}C$ for 4 days. The primed seeds were sown in soils with various soil moistures (60, 80, 100, 120, and 140% field capacity) at 17 and $25^{\circ}C$, respectively. Germination and emergence rates, plumule height, and radicle length of primed seeds were higher than those of untreated seeds at any soil moisture and temperature examined. The time from planting to 50% germination ($T_{50}$) of primed seeds was less than that of untreated seeds by 0.9～3.7 days. Germination rate, emergence rate, plumule height, and radicle length were highest at the soil moisture of 80% field capacity among the soil moistures. Priming effects of rice seeds on germination and emergence rates were more prominent under the unfavorable soil moistures (60, 100, 120, and 140% field capacity) than those under the optimum soil moisture condition (80% field capacity). However, priming effects on seedling growth were greater at near optimum soil moisture compared with too lower or higher soil moistures. Therefore, these findings suggest that priming of rice seeds may be a useful way for better seedling establishment under the adverse soil conditions.

• 한국작물학회지
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• 제46권4호
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• pp.325-327
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• 2001

Tobacco (Nicotiana tabacum L. cv KF109) seeds were artificially aged in a controlled environment of 45$^{\circ}C$ and 80% relative humidity condition for different duration up to 14 days before priming in polyethylene glycol 6000 solution of equivalent osmotic potential of -0.8 MPa for 8 days at $25^{\circ}C$. The seeds aged only and primed after aging were germinated at 15$^{\circ}C$ and $25^{\circ}C$ to observe the priming effects on the germination of aged seeds at different temperature. The germination percentage of the aged seeds was rapidly dropped starting from 8 days of aging and mean germination time (T$_{50}$) was greatly increased, particularly in germination at 15$^{\circ}C$. The germination capacity was greatly restored in the primed seeds after aging, particularly in the seeds of longer aging and germinating at 15$^{\circ}C$.>.

• Journal of Forest and Environmental Science
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• 제38권1호
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• pp.38-47
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• 2022

Hymenodictyon orixensis (Roxb.) Mabberley (locally known as Bhutum in Bangladesh) is both an ecologically and economically valuable multipurpose tree species for afforestation and reforestation programs in Bangladesh. Seed germination and seedling development study of H. orixense were conducted to find out the response to different growing medium, e.g., polybag (15×10 cm (T₀) and 20×15 cm (T₁)), sand medium in propagator house (T₂), conventional nursery bed (T₃), and root trainer (T₄) in the Nursery. Consequently, germination behavior and seedling morphological parameters of H. orixense were assessed. The results revealed that the sand medium of the propagator house (T₂) provided the highest germination % (58.57±22.30) and the highest germination energy (11.43±2.43) followed by seedlings growing in 20×15 cm polybags (T1) containing forest topsoil and cow-dung at a ratio of 3:1. Except for germination energy, germination values, and germination capacity, other seed biology parameters, particularly imbibition, germination period, germination rate, and plant survival percent in T₁, T₂, T₃, and T₄ were significantly (p<0.05) different from T₀. Each phenotypic parameter of seedlings and dry matter of shoot and root significantly differed from control except root length (p<0.992). Based on this study, Polybags of 20×15 cm size are regarded as the best medium for quality seedling development of H. orixense. The nursery bed (T₃) had the lowest germination performance and developed more inferior quality seedlings. Thereby, 20×15 cm size of polybags with conventional soil and cow-dung media is recommended for maximum germination and to grow the quality seedlings of H. orixense in the Nursery.

• Journal of Plant Biology
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• 제13권2호
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• pp.15-20
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• 1970

The treatment of X-ray was manipulated with the large focus of X-ray which was produced the capacity of 90 kilovoltage and 30 milliamphere in the distance of 50 centimeter. The germination of each gruop irradiated with X-ray for 5, 6, and 7 seconds was more delayed in the growth of carrot seed than those of the control group. However the germination of each group irradiated with X-ray for 2, 3, and 4 seconds was rather accelerated than those of the control group. The irradiation of ultraviolet light was carried out in the distance of 20 centimeter by Toshiba germidicial lamp which appeared the wavelength of 2537A$^{\circ}$. The germination of each group irradiated with ultraviolet light for 9, 15, 20, and 40 minutes was more delayed than those of the control group, but the germination of carrot seed was accelerated by adequate dosage of ultraviolet light. Consuquently it was suggested for the author that the inhibited substance contained in carrot seed would be certain fatty substance, becasue the germination of carrot seed was more accelerated by the treatment of acetone than those of X-ray and ultraviolet light.

• 한국약용작물학회지
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• 제24권3호
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• pp.177-182
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• 2016

Background: Many Taraxacum spp. plants are widely used in medicine, but some of them have propagation problems, such as strong dormancy and poor germination rates. This study investigated the effects of temperature, gibberellic acid ($GA_3$), and potassium nitrate ($KNO_3$) on seed germination in Taraxacum ohwianum. Methods and Results: The seeds (NIBRGR0000135524) were exalbuminous, and their length and width were $4.54{\pm}0.032mm$ and $0.97{\pm}0.029mm$, respectively. Various germination temperatures were tested (15, 20, 25, and $30^{\circ}C$), the optimum temperature for germination was found to be $20^{\circ}C$ (31.3%). High temperature ($30^{\circ}C$) resulted in non-typical seedlings (thickened and crumpled cotyledons, with a restricted root system). To increase the germination capacity, $GA_3$ (200, 500, and $1,000mg/{\ell}$) or $KNO_3$ (20, 50, 100, 200, and $500mg/{\ell}$) solutions were used as pre-soaking solutions instead of water. The $GA_3$ treatments increased the germination percentage and rate, but germination percentage was higher with the $KNO_3$ treatments. Under the $50-200mg/{\ell}\;KNO_3$ treatments, the germination percentage exceeded 80% after 12 days, and $50mg/{\ell}\;KNO_3$ was notably effective (91.2% after 15 days). Conclusions: T. ohwianum seeds showed improved germination at low temperatures. The best method for germination was presoaking in $50mg/{\ell}\;KNO_3$ for 24 h, in the dark at $4^{\circ}C$, and then incubating the germinated seed at $20^{\circ}C$ for 15 days.

• 한국환경과학회지
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• 제30권12호
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• pp.1041-1051
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• 2021

The purpose of this study was to identify for extenders, adhesives, colorants, and fungicides suitable for the production of high-quality film-coated seeds of Chinese cabbage. In addition, the effect of various additives used for film coating on the germination of coated seeds and seedling vigor was investigated to establish appropriate treatment conditions for film coating efficiency. The germination rate and germination speed in Chinese cabbage film-coated seeds did not differ significantly depending on the type and concentration of the extender, while the use of Talc resulted in superior granulation capacity and adhesion of the seed coat in comparison to Zeolite. There was no significant difference in the germination rate depending on the type of adhesive used for film coating of Chinese cabbage seeds, but the germination rate was slightly slower in the film-coated seeds using carboxymethyl cellulose as an adhesive. On the other hand, the seeds coated with a film using 1% PVA as an adhesive not only maintained hardness enough to withstand external pressure, but also showed a high germination rate. In addition, the suitable disinfectant for film coating was venoram, and the treatment concentration was good at 250 mg·L^-1. The type of colorant used in the film-coated seeds of Chinese cabbage did not significantly affect the germination rate, germination speed, and seedling vigor. The seeds film-coated using the liquid colorant were superior in appearance to the seeds film-coated with the solid colorant, which implies the possibility of reduction in the manufacturing costs by using the water-soluble paints as colorants for film-coated seeds.

• 한국산림과학회지
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• 제31권1호
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• pp.30-36
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• 1976

싸리류(類)의 종자저장기간별(種子貯藏期間別) 발아촉진처리별(發芽促進處理別) 발아율(發芽率)과 발아경과일수(發芽經過日數)를 조사(調査)하였든바 종자저장기간(種子貯藏期間) 40개월(個月)은 발아율(發芽率)이 30% 내외(內外)로 저조(低調)하였고 저장기간(貯藏期間) 28개월(個月)까지는 발아력(發芽力)에 큰 지장(支障)이 없었으며 발아촉진처리방법(發芽促進處理方法)으로는 80% 농류산(濃硫酸)에 3분처리(分處理) 및 과실중(果實中) 과피제거방법(果皮除去方法)이 가장 좋았다. 이들 처리(處理)가 타처리(他處理)에 비(比)하여 일주정도(一週程度) 빨리 발아율(發芽率)이 절정(絶頂)에 도달(到達)하였으며 종자저장기간(種子貯藏期間)이 단기(短期)인 것이 장기(長期)인 것에 비(比)하여 단시일내(短時日內)에 발아율(發芽率)이 절정(絶頂)에 도달(到達)하였다.